Not available / text

Not available / text

No description available.

This work was devoted to the study of (a) the physico-chemical, functional, and structural properties of bean (Phaseolus sp.) protein isolates in relation to their microstructures, and (b) the effects of protein carbohydrate interactions on physico-chemical, functional, and structural properties. The contents of protein, and both total and individual sugars of alkali extracted (amorphous) and citric acid extracted (bipyramidal and spheroidal) proteins from Phaseolus vulgaris (white kidney and navy) and Phaseolus lutanus (baby lima and large lima) beans were determined. The proteins were subjected to differential scanning calorimetry, and measurements of surface tension (air-water interface), surface hydrophobicity, and foam expansion. Structural analysis of the proteins were performed using Fourier transform infrared (FT-IR) spectroscopy. Enzymatic and chemical deglycosylation was performed on a white kidney bean protein isolate. / Glucose and mannose were the major sugars found in the isolates. Bipyramidal and spheroidal microstructures with higher protein contents generally had greater mannose content and lower glucose content. Differences in enthalpy of denaturation $( Delta$H), surface tension decay curves, surface hydrophobicities, and foam expansions were observed with isolates of different microstructures. Corresponding differences in molecular structure were not, however, detected by FT-IR spectroscopy. Using statistical analysis, a relationship between foam expansion and the $ Delta$H, solubility, surface hydrophobicity and surface tension of the isolates was obtained. Preliminary results suggest that the removal of carbohydrate influenced the physico-chemical properties of the protein.

Centrifugation of coconut milk resulted in cream, skim milk, and insoluble solids. Proteins were isolated from skim milk by the addition of acid, with or without heating. The separation and isolation gave the following coconut protein preparations: coconut milk, coconut skim milk, insoluble solids, acid precipitate, and acid-heat precipitate. / Trypsin inhibitory activity (TIA) of the coconut protein preparations was relatively low while tryptic digestibility of the isolated proteins was considerably lower than those of the coconut milk and skim milk, the digestibility of coconut protein preparations was lower than that of casein. In general, the emulsifying and farming properties of coconut protein preparations were lower than casein. The insoluble solids showed the highest viscosity when compared with the coconut protein preparations. In contrast to the whey protein concentrate (WPC), the apparent strain of gels from the acid precipitate increased as the pH increased. The gelation properties at pH 3 of the insoluble solids were better than WPC. / The estimated molecular weight by size-exclusion chromatography of coconut protein preparations gave 3 fractions with MW ranging from 6850 Da to 229402 Da. In native PAGE, coconut proteins were separated into at least 3 subunits and under SDS-denatured conditions, the major protein subunits showed MW of 54531 Da and 25008 Da, respectively. RP-HPLC separation of coconut milk, acid precipitate, and acid-heat precipitate gave 3 fractions containing several species of MW ranging between 35574 Da to 51209 Da when analyzed by mass spectometry.

No description available.

Bibliography: leaves 139-158. / vii, 158, [44] leaves, [33] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Hypothesises that p160 and p67 proteins repress the functional activities of c-Myb . Their ability to interact with the c-jun basic-leucine zipper (bZIP region) and their existence amongst the murine cell lines is examined. The complete cloning of cDNA encoding both p160 and p67, and the characterisation of these proteins, including the determination of their relationship is described. / Thesis (Ph.D.)--University of Adelaide, Dept. of Genetics, 1998?

The aim of this study was to initiate a structural analysis of the capsid polypeptides from several serotypes of bluetongue virus in order to provide insight into the relatedness and possible origins of the different serotypes. Tryptic peptide mapping of ¹²⁵I-labelled group antigen by ion exchange chromatography was used to assess the structural relatedness of seven BTV serotypes from Southern Africa, North America and Australia. Each serotype had several tyrosine containing tryptic peptides which were unique, but approximately 35% of the peptides analyzed were found to be highly conserved between all 7 serotypes. BTV-20 appeared to be closely related to BTV-B and these two serotypes with BTV-4 and BTV-17 appeared to form a closely knit central cluster. / KMBT_363 / Adobe Acrobat 9.53 Paper Capture Plug-in

Although numerous spider fibroins have been reported, no known silk coating peptides have been discovered. We provide the first biochemical evidence for a spider coating peptide, called SCP-2, found on gumfooted lines, scaffolding joints and egg cases. The presence of this spider coating peptide on the fibers is supported by MS/MS analysis. Using quantitative real-time PCR analysis, we also demonstrate that SCP-2 has a flagelliform-restricted mRNA pattern of expression. Molecular modeling of the SCP-2 amino acid sequence predicts it adopts an alpha-helical structure that is amphipathic in nature. SCP-2, which can be extracted from fibers using water, is hypothesized to influence the mechanical properties of the silk fibers as well as serve a protective function for the threads. Based upon the restricted pattern of expression of SCP-2, our findings reveal novel insight regarding the glandular function of the flagelliform gland in . cob weaving spiders, suggesting it produces aqueous coating materials that are deposited on a wide range of different silk types. In addition, in an attempt to advance our understanding regarding silk gene transcription, our lab has developed the first antibody against the bHLH factor SGSF. SGSF has been implicated as a potential transcriptional regulator of silk gene transcription in spiders. Development of the anti-SGSF antibody was accomplished via the overexpression and purification of a fusion protein in bacteria, which consisted of the C-terminal region of SGSF fused to thioredoxin. Purified SGSF fusion proteins were injected into rabbits and the polyclonal antiserum was collected and tested by western blot analysis to determine the specificity of the immunological reagent. Western blot analyses revealed the anti-SGSF antiserum was capable of recognizing bacterially expressed SGSF in an efficient manner. Collectively, these studies lay the groundwork for future investigations involving the use of the antibody to determine the role of SGSF in silk transcription.

No description available.