Thesis (M.S.)--University of Michigan, 1961.

Written for the Dept. of Chemistry. Title from title page of PDF (viewed 2009/06/11). Includes bibliographical references.

Thesis (M.S.)--University of Michigan, 1961.

Thesis (Ph. D.)--University of California, San Diego and San Diego State University, 2005. / Vita. Includes bibliographical references (leaves 138-152).

Thesis (Ph. D.)--Illinois State University, 1991. / Title from title page screen, viewed January 5, 2006. Dissertation Committee: Radheshyam K. Jayaswal, Brian J. Wilkinson (co-chairs), Herman E. Brockman, Anthony J. Otsuka, Hou Tak Cheung. Includes bibliographical references (leaves 117-129) and abstract. Also available in print.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1948. / Typescript. Vita. With this is bound: Studies on the hemorrhagic sweet clover disease : XIII. Anticoagulant activity and structure in the 4-hydroxycoumarin group / By Ralph S. Overman ... David G. Doherty, et. al. Reprinted from Journal of biological chemistry, vol. 153, no. 1 (Apr. 1944), p. 5-24. Includes bibliographical references.

Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisors: Christian P. Whitman and Brent L. Iverson. Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Chicago, Dept. of Biochemistry and Molecular Biology, March 2001. / Includes bibliographical references. Also available on the Internet.

The ubiquitin proteasome system targets proteins to the proteasome where they are degraded. Substrate recognition and processing prior to degradation take place at the 19S regulatory particle of the proteasome. A polyubiquitin chain, linked through isopeptide bonds formed between the C-terminal G76 and K48, is the signal responsible for delivery to the proteasome. Because chains linked via any of the seven lysine residues of ubiquitin exist in vivo and encode signals unrelated to protein degradation it is crucial for cells to avoid crosstalk between these different pathways. Several ubiquitin receptors related to proteasomal degradation have been identified but the selectivity between the different ubiquitin chains has not been assessed quantitatively while avoiding artefacts attributed to GST-dimerisation. By employing isothermal titration calorimetry, analytical ultracentrifugation and nuclear magnetic resonance, discrimination between K48- and K63-linked diubiquitin was established for the S. pombe proteasomal receptor Rpn10 and the shuttle protein Rhp23. The same methods allowed us to propose a discriminatory model for Rpn10. The crystal structures of the 19S regulatory particle subunits Rpn101-193 and Rpn121-224 have been determined and possible protein-protein interaction sites were identified by surface conservation and electrostatics analysis. Rpn12 surface residues were identified that had a negative effect on Rpn10-binding. This interaction was studied by surface plasmon resonance, fluorescence anisotropy and nuclear magnetic resonance. These experiments revealed a binding site on Rpn10 that is exclusively occupied by either ubiquitin or Rpn12 and for the first time demonstrated the interaction of a ubiquitin interacting motif with a protein other than ubiquitin.

Resumo: Todas as características de um organismo vivo são controladas pela expressão de genes específicos, e na qualidade da carne não é diferente. O presente estudo teve como objetivo correlacionar a expressão dos genes μ-Calpaína (CALP 1), m-Calpaína (CALP 2), Calpastatina (CAST), Tireoglobulina (TG), Diacilglicerol aciltransferase 1 (DGAT1) e a Leptina (LEP) com a qualidade da carne do Longissimus dorsi em dois grupos genéticos: Nelore (Bos indicus) e Canchim (5/8 de Bos taurus x 3/8 Bos indicus) em dois períodos (carne não maturada e com sete dias de maturação), e utilizá-la como marcador genético. Foram analisados 30 touros jovens, 15 Nelore e 15 Canchim, todos mantidos nas instalações do confinamento experimental da FMVZ-UNESP Botucatu, recebendo a mesma dieta e mesmo manejo. Alcançando peso de 370 kg e espessura de gordura de cobertura de 4 mm, foram abatidos e amostras do músculo Longissimus dorsi foram coletadas para análises de: lipídeos totais (LT), força de cisalhamento (FC), índice de fragmentação miofibrilar (MFI) e análise da expressão gênica por RT-qPCR. Entre as características de qualidade da carne, LT e MFI0 apresentaram diferença entre as raças (P<0,01), sendo MFI0 superior no Canchim e LT no Nelore. Quanto à expressão gênica, as CALPs não mostraram expressão diferencial entre os grupos (P>0,05), assim como DGAT1, TG e LEP. CAST foi mais expresso na raça Nelore (P<0,05). Quanto às correlações, foi observada apenas uma positiva entre DGAT1 e MFI0 (r=0,79, P<0,01) no Canchim. Diante dos resultados, sugere-se que a maior expressão de CAST no Nelore propiciou aos animais uma carne mais dura, quando comparados ao Canchim, e que a expressão gênica não pode ser usada como um marcador genético / Abstract: All the features of a living organism are controlled by the expression of specific genes, and the quality of the meat is no different. The present study was performed to correlate gene expression μ-calpain (CALP 1), m-calpain (CALP 2), calpastatin (CAST), thyroglobulin (TG), Diacylglycerol acyltransferase 1 (DGTA1) and leptin (LEP) with the meat quality of Longissimus dorsi muscle in two genetic groups: Nellore (Bos indicus) and Canchim (5/8 Bos taurus x 3/8 Bos indicus) in two periods, and use it as a genetic marker. We analyzed 30 young bulls, 15 and 15 Canchim Nellore. The animals were kept on the installation of the experimental confinement FMVZ-UNESP-Botucatu, receiving the same diet and management. When reached a weight of approximately 370 kg and a subcutaneous fat thickness of 4 mm, they were slaughtered and samples of the Longissimus dorsi muscle were collected for analysis of the: total lipids (TL), shear force (SF), myofibrillar fragmentation index (MFI) and analysis of gene expression by RT-qPCR. Among the characteristics of meat quality, LT and MFI0 showed differences between breeds (P<0,01), higher MFI0 in Canchim and LT higher in Nellore. Regarding gene expression, the CALPs showed no differential expression between groups (P>0.05), as well as DGAT1, TG, and LEP. CAST was more expressed in Nellore (P<0.05). Regarding the correlation was only observed a positive relationship between DGAT1 and MFI0 (r = 0.79, P <0.01) in Canchim. Considering the results, it is suggested that the increased expression of CAST in Nellore animals led to a tougher meat compared to Canchim, and that gene expression can not be used as a genetic marker / Orientador: Henrique Nunes de Oliveira / Coorientador: Maeli Dal Pai Silva / Banca: Simone Cristina Méo Niciura / Banca: Luiz Arthur de Loyola Chardulo / Mestre