A dissertation submitted in compliance with the requirements for the Masters Degree in Technology in Biotechnology, Durban University of Technology, 2011. / Under environmental stress, most microalgae produce intracellular neutral lipids as a storage mechanism. In the biotechnology industry, these lipids are extracted and converted to microalgal biodiesel; however the extent of the stress is not measured for optimum lipid accumulation. In the series of studies undertaken, Pulse Amplitude Modulated (PAM) fluorometry was used to measure three types of physiological stress on Chlorella sp., a freshwater microalgal species. Biomass and lipid yields were also used as indicators of the induced stress conditions. Firstly, nutrient induced physiological stress and the subsequent synthesis of cellular neutral lipids was investigated. / M

Master of Science / Department of Electrical and Computer Engineering / Steven Warren / A reference design for a wearable, wireless biomedical sensor set has been a long-term need for researchers at Kansas State University, driven by the idea that a basic set of sensor components could address the demands of multiple types of human and animal health monitoring scenarios if these components offered even basic reconfigurability. Such a reference design would also be a starting point to assess sensor performance and signal quality in the context of various biomedical research applications. This thesis describes the development of a set of wireless health monitoring sensors that can be used collectively as a data acquisition platform to provide biomedical research data and to serve as a baseline reference design for new sensor and system development. The host computer, an Intel Edison unit, offers plug-and-play usability and supports both Wi-Fi and Bluetooth wireless connectivity. The reference sensor set that accompanies the Intel Edison single-board computer includes an electrocardiograph, a pulse oximeter, and an accelerometer/gyrometer. All sensors are based on the same physical footprint and connector placement so that the sensors can be stacked to create a collection with a minimal volume and footprint. The latest hardware version is 3.1. Version 1.0 supported only a pulse oximeter, whereas version 2.0 included an electrocardiograph, pulse oximeter, and respiration belt. In version 3.0, the respiration belt was removed, and accelerometers and gyroscopes were added to the sensor set. Version 3.1 is a refined version of the latter design, where known hardware bugs were remedied. Future work includes the development of new sensors and casing designs that can hold these sensor stacks.

Wave dissipation was previously investigated intensively in the frequency domain, in which the dissipation of waves is described as attenuation of pressure pulse decay with respect to the frequency or harmonics. In this thesis, wave dissipation, including decay of pressure pulse, peak of wave intensity and wave energy, is investigated in the time domain using wave intensity analysis (WIA). Wave intensity analysis benefits to this research in several aspects including: 1) WIA allows for wave dissipation investigated in the time domain; 2) WIA does not make any assumptions about the tube's wall non-linearity and the analysis takes into account the effects of the vessel's wall viscoelastic properties, convective, frictional effects and fluid viscosity; 3) WIA offers a technique (separation) to study wave dissipation in one direction whilst taking into account the effect of reflections from the opposite direction; 4) The physical meaning of wave intensity provides a convenient method to study the dissipation of energy carried by the waves along flexible tubes. In this research, it is found that the degree of dissipation in flexible tube were not only affected by the mechanical properties of the wall property and viscosity of liquid but also by the other factors including initial pressure and pumping speed of piston as well as direction of wave in relation to direction of flow. Also an new technique to separate waves into forward and backward directions only using diameter and velocity might potentially be used to separate the waves in both directions non-invasively based on the non-invasive measurement of diameter (wall movement) available.

Neutral-point-clamped (NPC) three-level inverter is capable of handling higher dc bus voltage and producing output waveform of better quality than a conventional two-level inverter. The main objective of the present work is to analyze the existing PWM schemes for two-level and three-level inverters in terms of line current ripple, and to design new PWM techniques for the NPC inverter to reduce line current distortion. Various discontinuous PWM or bus-clamping PWM (BCPWM) methods for a two-level voltage source inverter are analyzed in terms of rms line current ripple, which is evaluated by integrating the error voltage (i.e. error between the applied and reference voltages). The BCPWM schemes can be broadly classified into continual-clamp PWM (CCPWM) and split-clamp PWM (SCPWM). It is shown that split-clamp PWM scheme leads to lower harmonic distortion than CCPWM scheme. Further, advanced bus-clamping PWM (ABCPWM) methods for a two-level inverter are also studied. These methods clamp each phase to the positive and negative DC terminals over certain intervals as in BCPWM schemes, and also switch each phase at double the nominal frequency in certain other intervals unlike in BCPWM. Analytical closed-form expressions are derived for the total rms harmonic distortion due to SCPWM, CCPWM and ABCPWM schemes. Existing sinusoidal and bus-clamping PWM schemes for three-level NPC inverters are also analyzed in the space vector domain. These methods are compared in terms of line current ripple analytically as well as experimentally. As earlier, closed-form expressions are derived for the harmonic distortion factors corresponding to centered space vector PWM (CSVPWM) and the various BCPWM methods. A three-level inverter can be viewed as an equivalent two-level inverter in each sixth of the fundamental cycle or hextant. This is widely used to simplify the control of an NPC inverter. Further, this approach makes it simple to extend the BCPWM and ABCPWM methods for two-level inverters to three-level inverters. Furthermore, the method of analysis of line current ripple for the two-level inverter can also be easily extended to the three-level case. The pivot vector, which is half the length of the longest voltage vectors produced by the NPC inverter, acts as an equivalent null vector for the conceptual two-level inverter. Each pivot vector can be produced by two inverter states termed as “pivot states”. Typically, in continuous modulation methods for NPC inverter such as sinusoidal PWM and centered space vector PWM, the switching sequence (i.e. the sequence in which the voltage vectors are applied) begins and ends with the same pivot vector in each subcycle, which is equivalent to a half-carrier cycle. To be more precise, the switching sequence starts with one pivot state and ends with the other in each subcycle. However, in case of BCPWM schemes, only one pivot state is used in a subcycle. The choice of pivot state results in a variety of BCPWM schemes for an NPC inverter. Different BCPWM schemes are evaluated in terms of rms line current ripple. The optimal BCPWM, which minimizes the rms current ripple, is determined for an NPC inverter, controlled as an equivalent two-level inverter. Further, four new switching sequences are proposed here for a three-level inverter, controlled as a conceptual two-level inverter. These sequences apply the pivot vector only once, but employ one of the other two vectors twice within the subcycle. These four switching sequences are termed as “ABCPWM sequences” for three-level inverter. These sequences exploit the flexibility available in the space vector approach to PWM to switch a phase more than once in a subcycle, which results in the application of an active vector twice within the subcycle. Influence of the proposed ABCPWM sequences on the line current ripple over a subcycle is studied. The various sequences are compared in terms of rms line current ripple over a subcycle. An analytical closed-form expression for rms line current ripple over a subcycle is derived in terms of reference magnitude, angle of reference voltage vector, and subcycle duration for each of the sequences. Further, closed-form expressions are also derived for the rms current ripple over a line cycle in terms of modulation index and subcycle duration, corresponding to the various sequences. The four proposed ABCPWM sequences for the NPC inverter can be grouped into two pairs of sequences. Each pair of sequences is shown to perform better than the individual sequences, if the two sequences are employed in appropriate spatial regions. Hence, with these two pairs of sequences, two hybrid PWM schemes are proposed. Finally, a hybrid PWM technique is proposed which employs all five sequences (conventional and proposed four sequences) in spatial regions where each performs the best. This is termed as “five-zone hybrid PWM”. The total harmonic distortion (THD) in the motor current, pertaining to all the proposed schemes, is studied theoretically over the entire range of linear modulation. The theoretical investigations are validated experimentally on a 2.2 kW, 415V, 4.9A, 50 Hz induction motor drive. The no-load current THD is measured over a range of fundamental frequency from 10 Hz to 50 Hz in steps of 2 Hz for the various PWM methods. Theoretical and experimental results bring out the reduction in current THD due to the proposed BCPWM schemes at fundamental frequencies of 45 Hz and above, compared to CSVPWM. The ABCPWM methods improve the performance at higher as well as lower modulation indices. Further improvement is achieved with the proposed five-zone hybrid PWM. At the rated frequency (50 Hz) of the drive, the improvement in line current distortion is around 36% with this hybrid PWM scheme over CSVPWM. The reduction in THD is also experimentally verified at different loads on the motor. The difference between the top and bottom capacitor voltages is measured at various operating conditions, corresponding to CSVPWM and the proposed schemes. No significant difference is observed in the dc neutral voltage shifts with the different proposed schemes and CSVPWM method. Thus, the proposed methods improve the THD at low and high speed ranges without appreciable worsening of the dc voltage unbalance.

In recent years there has been an increased drive towards point of care testing (POCT), in which assays are performed at the site of the patient. This has many benefits, critically; the time for a result to be obtained will be significantly reduced, allowing for greater and more effective decision making. Many currently used bioassay methods are not affordable in resource poor areas where infectious disease is most prevalent, in order to combat this issue many research groups are attempting to miniaturise equipment for portability and make assays more affordable and therefore more accessible. With the aims of generating a new assay platform which is highly portable and affordable, the work in this thesis presents the development of several generic methods utilising nano- and micro-scale beads coated with aptamer which are then monitored interacting with target proteins with Tunable Resistive Pulse Sensing (TRPS). Aptamers are short oligonucleotide sequences which are capable of binding to a wide range of targets with high selectivity and comparable affinity to antibodies while possessing greater stability and have begun to challenge the role of antibodies. When aptamers bind a target, they often undergo a conformational change. In the assays described herein, this conformational change is key to the observed signal changes. TRPS is a pore-based system in which beads moving through a pore cause a measurable increase in resistance which can be used to derive particle size, concentration, and mobility. During the course of this thesis several template TRPS aptasensors have been developed. TRPS was successfully used to confirm the successful coating of nano- and micro-scale beads with DNA aptamers by monitoring an increase in electrophoretic mobility when the negatively charged DNA is added to the surface. Following on from this, TRPS was used to monitor the interaction of aptamer tagged beads with thrombin protein enabling thrombin detection down to 1.4 nM and the comparison of several thrombin-aptamers with results comparable to previously published SPR data. Thrombin was postulated to shield the negative DNA, resulting in a decrease in mobility, and the magnitude of this charge shielding was found to depend upon the binding mechanism of the aptamer used. This effect is not thought to be specific to our system nor to thrombin, the principles outlined here may be applied to other RPS technologies, or by interchanging of the aptamer, different proteins. In later chapters, this method is expanded to include multiplexed detection of growth factors and a significant improvement in signal. vi Following on from this, the controlled aggregation of avidin coated beads in the presence of biotinylated-BSA was explored. Factors impacting upon this assay were discussed including magnetic separation, particle size and particle concentration, and different methods of data interpretation were presented. This aggregation study identified several key parameters in the use of TRPS in aggregation assays. Using the methods outlined by the study of aggregates, a dispersion assay was then designed in which the interaction of thrombin proteins with clusters of particles brought about the release of many small particles by the disruption of double stranded DNA linkages. This dispersion assay incorporated magnetic separation to simplify the read-out and relied on measuring particle concentration rather than mobility, enabling the use of additional pressure to increase speed and ease of use. Using this method, thrombin was able to be detected down to 100 fM, a significant advancement in TRPS aptasensors.

Les systèmes radar nécessitent d’être de plus en plus performants et doivent émettre des impulsions les plus identiques possibles. Un critère permet de quantifier la bonne régularité des impulsions radar au cours du temps : la stabilité pulse à pulse. L’amplificateur de puissance est un élément essentiel du système radar. Dans ce sens, ce travail présente une analyse du critère de stabilité pulse à pulse dans le cas d’un amplificateur HEMT GaN. Les formules mathématiques permettant d’extraire la valeur de la stabilité pulse à pulse des mesures temporelles d’enveloppe sont présentées. La conception et la réalisation d’un amplificateur de puissance RF connectorisé 50 Ω sont décrites. Divers cas de rafales radar ont été étudiés au travers des mesures temporelles d’enveloppe pour en quantifier l’impact sur les valeurs de stabilité pulse à pulse. Un banc de mesure hétérodyne de la stabilité pulse à pulse a été spécialement développé pendant ces travaux de thèse. Finalement, ces résultats de stabilité pulse à pulse ont été utilisés pour optimiser le modèle électrique non linéaire du transistor HEMT GaN afin de prendre en compte lors des simulations temporelles d’enveloppe les effets de la thermique et des pièges. / Radar-oriented applications require stringent performances. Among them, emitting pulse train with uniform envelope characteristics in term of amplitude and phase. The criterion to quantify the self-consistency of radar signals over the pulse train is the pulse to pulse stability. The power amplifier is the most critical element in the RF radar chain because it has a strong impact on the overall pulse to pulse stability performances. In this context, this work is focused on the study of the impact of a HEMT GaN power amplifier on the pulse to pulse stability. Mathematical approach is presented to derive the pulse to pulse stability from time domain envelope measurements. Design and implementation of a 50Ω matched RF power amplifier are presented. Different radar bursts scenario are investigated and their impact on the pulse to pulse stability are quantified through extensive time domain envelope measurements. For that purpose, a dedicated experimental heterodyne time domain envelope test bench has been developed. These pulse to pulse stability measurements are finally used to optimize and fully validate a nonlinear electrical model of a HEMT GaN, allowing to quantify the relative impact of thermal and trapping effects during circuit envelope simulation in radar-oriented applications.

Swath bathymetry sidescan (SBS) sonar and the angle-of-arrival processing that underlies these systems has the capability to produce much higher resolution three dimensional imagery and bathymetry than traditional beamformed approaches. However, the performance of these high resolution systems is limited by signal-to-noise ratio (SNR) and they are also susceptible to multipath interference. This thesis explores two methods for increasing SNR and mitigating multipath interference for SBS systems. The first, binary coded pulse transmission and pulse compression is shown to increase the SNR and in turn provide reduced angle variance in SBS systems. The second, transmit beamforming, and more specifically steering and shading, is shown to increase both acoustic power in the water and directivity of the transmitted acoustic radiation. The transmit beamforming benefits are achieved by making use of the 8-element linear angle-of-arrival array typical in SBS sonars, but previously not utilized for transmit. Both simulations and real world SBS experiments are devised and conducted and it is shown that in practice pulse compression increases the SNR, and that transmit beamforming increases backscatter intensity and reduces the intensity of interfering multipaths. The improvement in achievable SNR and the reduction in multipath interference provided by the contributions in this thesis further strengthens the importance of SBS systems and angle-of-arrival based processing, as an alternative to beamforming, in underwater three dimensional imaging and mapping. / Graduate / 0544 / 0547 / mark.butowski@gmail.com

Please read the abstract in the section 00front of this document / Dissertation (MSc Agric (Horticulture))--University of Pretoria, 2006. / Plant Production and Soil Science / unrestricted

Conventional gas turbine combustors operate with a designed drop in pressure over the length of the device. This is desired in order to encourage mixing within the combustor. Compared to this, pulse pressure gain combustors are an alternative to the conventional combustor that produces an increase in static pressure between the inlet and exhaust of the device. The removal of the combustor pressure loss increases the efficiency of the combustion process by increasing the amount of work produced. Many types of pulsed pressure gain combustors exist. Of these, the valveless pulse combustor is the simplest featuring no moving parts. Whilst some research has been conducted into investigating the performance and workings of a pulse combustor, little has been conducted with the view of cooling the combustor. This has been the focus for the research contained herein. The research has focussed on establishing an understanding of the heat transfer characteristics within a pulse combustor tailpipe. This has involved experimental, analytical and computational research on a pulse combustor as well as on a cold-flow model of a pulse combustor tailpipe. This has enabled a study into the feasibility of cooling a pulse combustor to be conducted. The research has found that for conditions where the unsteady velocity amplitude within the cold-flow model of the pulse combustor tailpipe exceeds the mean velocity, an enhancement to the heat transfer coefficient is measured compared to the value expected in a similar non-oscillating flow. When there is no enhancement to the heat transfer coefficient, the cyclic variation of the unsteady heat flux follows the variation of the unsteady pressure within the device. However, at times of enhancement, the instantaneous heat flux structure shows a large deviation from the structure of the pressure field driving the oscillations. This change is shown to be caused by the reversal in the near-wall velocity and may indicate a mechanism for the enhancement in the mean heat flux. The cooling feasibility study showed that with further investigation, it may be possible to cool a pulse combustor within a gas turbine engine.

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