Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2018-09-10T11:45:29Z No. of bitstreams: 1 DEONILSON_GHIZONI_SCHMOELLER.pdf: 2264316 bytes, checksum: dc3d305d95ec84ddc7eb55024a737758 (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-09-11T13:47:45Z (GMT) No. of bitstreams: 1 DEONILSON_GHIZONI_SCHMOELLER.pdf: 2264316 bytes, checksum: dc3d305d95ec84ddc7eb55024a737758 (MD5) / Made available in DSpace on 2018-09-11T14:50:09Z (GMT). No. of bitstreams: 1 DEONILSON_GHIZONI_SCHMOELLER.pdf: 2264316 bytes, checksum: dc3d305d95ec84ddc7eb55024a737758 (MD5) Previous issue date: 2017-12-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Introduction: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease of unknown origin that can cause inflammatory lesions in various organs. In recent years, platelet-related functions have been assigned in the pathophysiology of autoimmunity, inflammation, atherosclerosis and cancer immunology. The role of platelets as a marker of inflammation in SLE is an area of interest today. Mean platelet volume (MPV) has been the most studied index in autoimmune diseases recently, but the use of the immature platelet fraction (IPF) may bring new insights into the knowledge of platelet activity in SLE and we do not yet have a description of its behavior in SLE. Objective: To quantify platelet indexes (platelet, MPV and IPF) in patients with active and inactive SLE compared to healthy blood bank controls. In parallel, correlate the indices with each other and with laboratory abnormalities of the disease. Methods: Cross-sectional, controlled study. Adults with SLE according to the ACR 1997 criteria and healthy blood bank controls made up the groups. The platelet and MPV were obtained in automated counter XE-5000. The IPF was expressed as a percentage by flow cytometry, and cells with fluorescence for cytoplasmic RNA were positive. Results: Forty-five patients with SLE (30 inactive) and 257 controls were evaluated. The median IPF was significantly higher in active SLE than in healthy controls (p = 0.032). There was a significant correlation between IPF and MVP in the lupus population (p <0.001, rs = 0.519) and controls (p <0.001, rs = 0.845). An inverse correlation between IPF and platelet counts was observed in active lupus (p = 0.025; rs = 0.575). There was no association between IPF and presence of anti-DNA. Conclusion: The results of our data point to elevated IPF in patients with active SLE compared to healthy controls. The inverse correlation of IPF with plateletometry in active SLE is instigating finding and may stimulate further research. The role of IPF as an inflammatory marker in SLE demands future confirmation. / Introdu??o: O l?pus eritematoso sist?mico (LES) ? uma doen?a autoimune cr?nica de origem ainda desconhecida, podendo causar les?es inflamat?rias em diversos ?rg?os. Nos ?ltimos anos, t?m sido atribu?do ?s plaquetas fun??es relacionadas na fisiopatogenia da autoimunidade, inflama??o, aterosclerose e imunologia do c?ncer. O papel das plaquetas como marcador de inflama??o no LES constitui ?rea de interesse na atualidade. O volume plaquet?rio m?dio (VPM) tem sido o ?ndice mais estudado nas doen?as autoimunes recentemente, mas o uso do ?ndice de plaquetas imaturas (IPI) pode trazer novas luzes no conhecimento da atividade plaquet?ria no LES e ainda n?o temos descri??o do seu comportamento no LES. Objetivo: Quantificar os ?ndices plaquet?rios (plaquetometria, VPM e IPI) em pacientes com LES ativo e inativo comparativamente a controles sadios de banco de sangue. Em paralelo, correlacionar os ?ndices entre si e com altera??es laboratoriais da doen?a. M?todos: Estudo transversal, controlado. Adultos com LES de acordo com os crit?rios do ACR 1997 e controles sadios de banco de sangue compuseram os grupos. A plaquetometria e o VPM foram obtidos em contador automatizado XE-5000. O IPI foi expresso de forma percentual por citometria de fluxo, sendo positivas as c?lulas com fluoresc?ncia para RNA citopl?smico. Resultados: Quarenta e cinco pacientes com LES (30 inativos) e 257 controles foram avaliados. A mediana do IPI foi significativamente maior no LES ativo do que em controles sadios (p=0,032). Houve correla??o significativa do IPI com o VPM na popula??o l?pica (p<0,001; rs=0,519) e nos controles (p<0,001, rs=0,845). Uma correla??o inversa entre IPI e plaquetometria foi observada nos l?picos ativos (p=0,025; rs=0,575). N?o houve associa??o entre IPI e presen?a de anti-DNA. Conclus?o: Os resultados do nosso estudo apontam para IPI elevado em pacientes com LES ativo comparativamente a controles sadios. A correla??o inversa do IPI com plaquetometria no LES ativo ? um achado instigante e podeestimular novas pesquisas. O papel do IPI como marcador inflamat?rio no LES demanda confirma??o futura.

Made available in DSpace on 2014-12-17T15:38:42Z (GMT). No. of bitstreams: 1 AdrianaDA.pdf: 830797 bytes, checksum: e4e3ffec1d282c562c0d8311c0e7dfd6 (MD5) Previous issue date: 2004-02-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Systemic lupus erythematous (SLE) is a chronic and auto-immune disease that can affect several systems of one?s body, including the nervous system, causing several clinical evidences, which can put in risk the person?s life. Although the illness could manifest itself at any age or sex, studies indicate higher incidence among women. Its etiology points to the combination of genetic, hormonal and environmental factors. Due to the disease?s complexity, it is evident that it affects all the person?s life as a whole and not only its organic dimension. It is believed that the signification attributed to all the process of sickening influences its treatment, as well as the person?s capacity to cope with the difficulties and implicit profits involved in the process.In this study, eight women who were affected by SLE were interviewed, with the aim of examining carefully the processes of signification as well as the generation of meanings which permeate these women?s sickening processes. The analysis of their speeches evidences distinct forms of giving meaning to the process, regardless of the time of the diagnosis. The fact that the disease is incurable was shocking to all the participants, and it demanded changes in their lives, in order to detain a relative control of their condition. The majority of the participants were able to deal with these modifications, since strategies have been created to face the difficulties and thus to preserve their social life, without damaging their health. However, some of the participants did not obtain strenght to cope with the disease, eventually developing a depressive state. It is observed that not only SLE has innumerable ways of manifestation, but the experience of the illness is very subjective and dynamic. There are also several ways of expressing this experience, according to the implications in the social, cultural and economic context where the participants are inserted. This ratifies the necessity of a interdisciplinary approach to embrace SLE complexity. (310 words, 1.610 characters) / O l?pus eritematoso sist?mico (LES) ? uma doen?a cr?nica e auto-imune, que pode atingir v?rios ?rg?os e sistemas do corpo, incluindo o sistema nervoso, gerando diversos quadros cl?nicos que se tornam uma amea?a ? vida da pessoa. Embora a doen?a se manifeste em qualquer idade e sexo, estudos indicam maior incid?ncia entre as mulheres. Sua etiologia aponta para a combina??o de fatores gen?ticos, hormonais e ambientais. Devido ? sua complexidade, fica expl?cito que afeta toda a vida da pessoa e n?o apenas a dimens?o org?nica. Acredita-se que os sentidos atribu?dos a todo o processo de adoecer, influenciam no tratamento do LES e na forma da lidar com as dificuldades e percal?os impl?citos nesse processo. Foram assim realizadas entrevistas em profundidade com oito mulheres portadoras de LES objetivando aprofundar nos processos de significa??o e gera??o de sentidos permeando o processo de adoecer dessas participantes. A an?lise dos discursos evidencia distintas formas de dar sentido ao adoecer, n?o sendo significativo o tempo de diagn?stico nesse processo. O fato da doen?a n?o ter cura foi um choque para todas as participantes, exigindo mudan?as em suas vidas, a fim de manter um relativo controle da mesma. A maioria conseguiu lidar com essas modifica??es, criando estrat?gias para enfrentar as dificuldades e assim preservar sua vida social, sem trazer preju?zo a sua sa?de. Entretanto, outras, n?o conseguem potencializar for?as para conviver com a doen?a manifestando um constante estado depressivo. Observa-se assim que n?o ? s? o LES que tem in?meras maneiras de se apresentar, mas a experi?ncia da doen?a ? algo muito subjetivo e din?mico, tendo tamb?m diversas formas de express?o conforme as conseq??ncias advindas desse processo e suas implica??es a partir do contexto social, cultural e econ?mico em que as participantes est?o inseridas, ratificando assim, a necessidade de uma abordagem interdisciplinar que abarque essa complexidade

Made available in DSpace on 2014-12-17T14:16:23Z (GMT). No. of bitstreams: 1 TatianaXC.pdf: 546242 bytes, checksum: 32d2bd23fe599a131f51f3299a41cc73 (MD5) Previous issue date: 2009-03-27 / OBJECTIVE: The aim of this work was to analyse some oxidative stress parameters in patients of Systemic L?pus Erythematosus. PATIENTS AND METHODS: Determinations of reduced glutathione content in whole blood were carried out. The activity of superoxide dismutase, gluthatione peroxidase and catalase in erythrocytes and the concentration of reactive substances of acid thiobarbituric in plasma of patients female (n =19) with SLE no activity of disease (Mex-SLEDAI < 2), with average ages of 32 ? 11 years, through the spectrophotometrical methods and from healthy individuals (n =30). Statistical data were analyzed by student t-test, p<0,05. RESULTS: Our data indicated a significant decrease on the activity of catalase and significant increase on the concentration of reactive substances of acid thiobarbituric in patients with SLE comparing with healthy individuals. There was no significant difference in other parameters. CONCLUSION: The results showed that oxidative stress has a role in the pathogenesis of the disease in SLE, even in patients without active disease. / OBJETIVO: Analisar os par?metros de estresse oxidativo no L?pus Eritematoso Sist?mico. PACIENTES E M?TODOS: Foram realizadas as determina??es do conte?do de glutationa reduzida no sangue total, da atividade da super?xido dismutase, da glutationa peroxidase e da catalase nos eritr?citos e da concentra??o de Subst?ncias Reativas ao ?cido Tiobarbit?rico no plasma de 19 pacientes do sexo feminino com LES sem atividade de doen?a (Mex-SLEDAI < 2), com m?dia de idade de 32 ? 11 anos, atrav?s de m?todos espectrofotom?tricos e comparada a um grupo de 30 indiv?duos sadios nas mesmas condi??es. As pacientes com LES apresentaram tempo de doen?a em torno de 5 ? 3 anos. Os dados estat?sticos foram analisados atrav?s do teste tstudent considerando um n?vel de signific?ncia de P<0,05. RESULTADOS: Foi verificado que a concentra??o de Subst?ncias Reativas ao ?cido Tiobarbit?rico foi significantemente mais elevada e a atividade da catalase foi significantemente diminu?da nos pacientes com LES em compara??o ao controle. N?o houve diferen?a significativa nos outros par?metros. CONCLUS?O: O aumento da peroxida??o lip?dica e a diminui??o da atividade da catalase sugerem que o estresse oxidativo tem um papel na patog?nese da doen?a no LES, mesmo em pacientes sem doen?a ativa

Ce travail de thèse porte sur l’étudephoto-physique de chromophores donneur-π-accepteur utilisés dans les cellules solaires àcolorant. Après photo-excitation, ces moléculessubissent des processus de transfert de chargeintramoléculaire (ICT), de solvatation etd’injection de charges qui se déroulent dans ledomaine temporel de la picoseconde. Or touterelaxation énergétique de l’état excité diminue lacinétique de l’injection de charges.Des mesures de spectroscopie stationnaire et defluorescence résolue en temps (de la femto- à lananoseconde) ont permis de résoudre ladynamique de relaxation de ces colorants ensolution, sur films mésoporeux et en cellulescomplètes.Les dynamiques de relaxation spectrale etd’inhibition de l’intensité de fluorescence del’état excité ont pu être caractériséesindépendamment et mettent en évidence unchangement d’état électronique à l’état excité.Les trois colorants étudiés évoluent vers un étatà transfert de charge (état CT). Un tel étatinteragit fortement avec son environnement.Afin d’étudier l’injection de charge dans lesoxydes mésoporeux semi-conducteurs, unmontage expérimental de fluorescence résolue entemps destiné à l’étude de systèmes opaques etdiffusifs a été réalisé et est décrit dans cette thèse.Ce montage possède une résolution temporelled’environ 300 fs.Les résultats mettent en évidence la présenced’une compétition entre l’injection de charges etla relaxation de l’état excité dans les cellulescomplètes. / This thesis deals with thephotophysical study of donor-π-acceptorchromophores used in dye sensitized solar cells.After photo-excitation, these molecules undergointramolecular charge transfer (ICT), solvationand charge injection processes that take place inthe picosecond time domain. However, anyenergetic relaxation of the excited statedecreases the injection kinetics.Steady-state spectroscopy and time-resolvedfluorescence measurements (from the femto- tothe nanosecond) were used to solve therelaxation dynamics of these dyes in solution, onmesoporous films and in complete cells.The spectral relaxation and fluorescencequenching dynamics of the excited state couldbe characterized independently and highlight anelectronic relaxation at the excited state. Thethree dyes studied evolve towards a chargetransfer state (CT state). Such a state is in stronginteraction with its environment.To study charge injection in semiconductormesoporous oxides, a time-resolvedfluorescence set-up for the study of opaque anddiffusive systems has been implemented and isdescribed in this thesis. It provides a timeresolution of about 300 fs.The results demonstrate the existence of acompetition between the injection of chargesand the relaxation of the excited state in thecomplete cells

Made available in DSpace on 2017-07-24T19:22:33Z (GMT). No. of bitstreams: 1 Johanna Lizbeth Cuadros Sanchez.pdf: 3888267 bytes, checksum: abbdebf22c3a3a8b49c91f6bc734b34d (MD5) Previous issue date: 2014-02-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Abstract: Luting of posts inside the root canal is still a challenge because of the difficult to bonding mainly in the apical third. The aim of this study was evaluate the influence of the application of 3 simplified total-etch adhesive systems (Adper Single Bond 2 Ambar, and XP-Bond+self-cure activator) under manual mode or sonic vibration on the push-out bond strength (POBS), silver nitrate uptake (SNU) and in situ degree of conversion (ISDC) associated to resin cement when used for luted posts. Methods: The roots of 66 human premolars were prepared and divided into six groups (n=11), according to the following combination: adhesive and application mode. The posts were cemented in accordance with the manufacturer’s instructions of each cementation system. After water storage, the roots were sectioned transversely into six slabs and it were tested in POBS (0.5mm/min). Two roots for group were data were immersed in 50% silver nitrate, photo-developed and analyzed by SEM to measure the SNU. Two roots for group were evaluated by micro-Raman spectroscopy to measure ISDC. The data were analyzed by 3-way repeated measures ANOVA and Tukey's tests (5%). Results: When sonic application was performed, usually there are a significantly increasing on the POBS (MPa) (35% and 37% respectively) and ISDC (%) (14% and 38% respectively in the medium and apical thirds) and decreasing of SNU (%) for all groups mainly in the medium (64% decreasing) and apical (84%) thirds when compared with manually application (P < 0.05). / Resumo: A cimentação de pinos dentro do canal radicular ainda é um desafio por causa das dificuldades relativas à adesão, principalmente no terço apical. O objetivo deste estudo foi avaliar a influência da aplicação de três sistemas adesivos convencionais simplificados (Adper Single Bond 2, Ambar e XP-Bond + self cure ativator) manualmente ou através de um dispositivo sônico vibratório na resistência de união por push-out (RU), na infiltração de nitrato de prata (NI) e no grau de conversão in situ (GC) associado ao cimento resinoso de cada fabricante, quando utilizado para cimentação de pinos. Métodos: As raízes de 66 pré-molares humanos foram preparadas e divididas em seis grupos (n = 11), de acordo com a seguinte combinação: adesivo/cimento e modo de aplicação. Os pinos foram cimentados de acordo com as instruções do fabricante de cada sistema de cimentação apenas variando o modo de aplicação. Depois do armazenamento em água, as raízes foram seccionadas transversalmente em seis fatias e foram testados em RU (0,5 mm/min). Duas raízes por grupo foram imersas em 50% de nitrato de prata, e após revelação, foram analisadas por MEV para medir a NI. Duas raízes por grupo foram avaliadas por espectroscopia de micro-Raman para medir GC. Os dados foram analisados por análise de variância de 3 fatores de medidas repetidas (terço radicular) e teste de Tukey (5%). Resultados: Quando a aplicação sônica vibratória foi utilizada, em geral, se observou um aumento significativo na RU (MPa) (35% e 37% respectivamente) e GC (%) (14% e 38% respectivamente nos terços médio e apical) e diminuição da NI (%) para todos os grupos, principalmente nos terços médio (redução de 64%) e apicais (84%) quando comparada com a aplicação manual (p <0,05) e este fenômeno ocorreu para todos os sistemas adesivos/cimentos testados.