The random-coil model has been dominant for unfolded proteins since the 1950’s; however, some experiments showed that the unfolded proteins were biased toward specific conformations in conflict with the random-coil model. Recently, residual dipolar couplings (RDCs) and paramagnetic relaxation enhancement (PRE) were applied to obtain a large amount of structural information on unfolded proteins. Typically, these data were interpreted in a framework of random-coil ensembles with a good agreement between experimental data and theoretical predictions. In this thesis, it was tested whether locally organized nonrandom ensembles could describe this agreement equally as well. Using a complete set of RDC and PRE data for denatured ubiquitin, it was revealed that there was no distinguishable difference between random-coil ensembles and ensembles containing 50% native structure. Thus, while it is important to measure as many RDCs or PRE as possible, even the best datasets may be insensitive to local organization in unfolded proteins.

No description available.

The detonation-based engine has the key advantage of increased thermodynamic efficiency over the traditional constant pressure combustor. These detonation-based engines are also known as Pressure Gain Combustion systems (PGC) and Rotating Detonation Combustor (RDC) is a form of PGC, in which the detonation wave propagates azimuthally around an annular combustor. Prior researchers have performed a high fidelity 3-D numerical simulation of a rotating detonation combustor (RDC) to understand the flow physics such as detonation wave velocity, pressure profile, wave structure; however, performing these 3-D simulations is computationally expensive. 2-D simulations are a potential alternative to reduce computational cost. In most RDCs, fuel and oxidizer are injected discretely from separate plenums, and this discrete fuel/air injection results in inhomogeneous mixing within the domain. Due to the discrete fuel injection locations, fuel/oxidizer will stratify to form localized pockets of rich and lean mixtures. The motivation of the present study is to investigate the impact of unmixedness and stratification length scales on the performance of an RDC using a 2-D numerical approach. Unmixedness, which is defined as the standard deviation of equivalence ratio normalized by the mean global equivalence ratio, is a measure of the degree of fuel-oxidizer inhomogeneity. To model the effect of unmixedness in a 2-D domain, a lognormal distribution of the fuel mass fraction is generated with a mean equivalence ratio of 1 and varying standard deviations at the inlet boundary as a numerical source term. Moreover, to model the effects of stratification length scales, fuel mass fraction at the inlet boundary cells is bundled for a given length scale, and the mass fractions for these bundles are updated based on the lognormal distribution after every three-time steps. Using this methodology, 2-D numerical analyses are carried out to investigate the performance of an RDC for an H2-air mixture with varying unmixedness and stratification length scales. Results show that mean detonation velocity decreases and wave speed variation increases with an increase in unmixedness. However, with an increase in stratification length scale mean velocity remain relatively unchanged but variation in local velocity increases. The detonation wave front corrugation also increases with an increase in mixture inhomogeneity. The mean detonation cell size increases with an increase in unmixedness. The cell shape becomes more distorted and irregular with an increase in stratification length scale and unmixedness. The combined effect of unmixedness and stratification length scale leads to a decrease in pressure gain. Overall, this concept is able to elucidate the effects of varying unmixedness and stratification length scales on the performance of an RDC. / Master of Science / Pressure Gain Combustion (PGC) system has gained significant focus in recent years due to its increased thermodynamic efficiency over a constant pressure Brayton Cycle. Rotating Detonation Combustor (RDC) is a type of PGC system, which is thermodynamically more efficient than the conventional gas turbine combustor. One of the main aspects of the detonation process is the rapid burning of the fuel-oxidizer mixture, which occurs so fast that there is not enough time for pressure to equilibrate. Therefore, the process is thermodynamically closer to a constant volume process rather than a constant pressure process. A constant volume cycle is thermodynamically more efficient than a constant pressure Brayton cycle. In an RDC, a mixture of fuel and air is injected axially, and a detonation wave propagates continuously through the circumferential section. Numerical simulation of an RDC provides additional flexibility over experiments in understanding the flow physics, detonation wave structure, and analyzing the physical and chemical processes involved in the detonation cycle. Prior researchers have utilized a full-scale 3-D numerical simulation for understanding the performance of an RDC. However, the major challenge with 3-D analyses is the computational expense. Thus, to overcome this, an inexpensive 2-D simulation is used to model the flow physics of an RDC. In most RDCs, the fuel and oxidizer are injected discretely from separate plenums. Due to the discrete fuel injection, the fuel/air mixture is never perfectly premixed and results in a stratified flow field. The objective of the current work is to develop a novel approach to independently investigate the effects of varying unmixedness and stratification length scales on RDC performance using a 2-D simulation.

Disfunções Temporomandibulares e Dor Orofacial ou DTM, referem-se a um conjunto de alterações funcionais e/ou estruturais que se manifestam nas articulações temporomandibulares (ATMs), nos músculos da mastigação e nas estruturas associadas que compõem o aparelho mastigatório (Okeson, 2013). A DTM tem o potencial de não só interferir nas atividades funcionais do sistema estomatognático, mas também de afetar o comportamento social e psicológico do indivíduo. Este trabalho avaliou a amplitude dos movimentos mandibulares e a dor a palpação muscular no pré e pós-tratamento de participantes com DTM. Os participantes incluídos na pesquisa responderam ao Research Diagnostic for Temporomandibular Dysfunction (RDC/TMD) para viabilizar a formulação do diagnóstico de DTM e qualificar a dor a palpação dos músculos faciais, e submetidos à análise dos movimentos através de uma análise 3D, o JMA que avalia os movimentos mandibulares por vias ultrassônicas, no pré e póstratamento com placa estabilizadora por 30 e 60 dias. Os participantes foram reavaliados, com o objetivo de saber se haverá uma melhora na amplitude dos movimentos mandibulares pela abertura, lateralidades e protrusão e da movimentação condilar dentro da cavidade articular no plano sagital e frontal. Os resultados demonstraram não ter diferenças estatísticas na maior parte dos movimentos excursivos mandibulares (abertura, lateralidade e protrusão) avaliados tanto pelo sistema de ultrassom JMA quanto pelo exame do RDC/TMD. Na análise descritiva dos resultados obtidos através da palpação dos músculos na aplicação do RDC/TMD observamos uma grande melhora no quadro clinico de dor a palpação após 60 dias de uso da Placa Oclusal estabilizadora. Também não observamos diferenças estatisticamente significantes entre os dados coletados de amplitude dos movimentos pelo sistema informatizado JMA e pelo questionário RDC/TMD, ou seja, podemos afirmar que a avaliação pelo JMA é precisa e confiável. / Temporomandibular Disorders and Orofacial Pain or TMD, refer to a set of functional and / or structural changes that are manifested in the temporomandibular joints (TMJ) in the masticatory muscles and associated structures that make up the masticatory system (Okeson, 2013). The TMD has the potential to not only interfere with the functional activities of the stomatognathic system, but also affect the social and psychological behavior of the individual. This study evaluated the range of mandibular movements and muscle palpation pain in the pre and post-treatment of participants with TMD. Participants included in the study responded to the Research Diagnostic for Temporomandibular Dysfunction (RDC / TMD) to allow the formulation of a diagnosis of TMD and qualify pain palpation of the facial muscles, and subjected to analysis of movement through 3D JMA assessing movements mandibular by ultrasonic means, before and after treatment with stabilizing plate for 30 and 60 days. Participants were reassessed in order to know if there will be an improvement in the range of mandibular movements for opening, laterality and protrusion and condyle movement within the joint cavity in the sagittal and frontal plane. The results showed no statistical differences have in most excursive mandibular movements (opening, lateral and protrusive) assessed both by JMA ultrasound system as the examination of the RDC / TMD. In the descriptive analysis of the results obtained by palpation of the muscles in the application of the RDC / TMD noticed a great improvement in the clinical picture of pain palpation after 60 days of use board stabilizing occlusal. We did not observe statistically significant differences between the data collected from the range of motion by the computerized system JMA and the questionnaire RDC / TMD, other words, we can say that the evaluation by the JMA is accurate and reliable.

O presente estudo avaliou a relação dos parâmetros do eixo II dos Critérios de Diagnóstico de Pesquisa das Disfunções Temporomandibulares (RDC/TMD) na resposta terapêutica de pacientes com DTM. Trinta e três pacientes (média de idade de 33,2 anos ± 13,4) com artralgia da articulação temporomandibular (ATM) foram submetidos a três terapêuticas: laser de baixa potência (LBP) + piroxicam (LPi), LBP + placebo de piroxicam (L) e piroxicam + placebo de LBP (Pi). Os pacientes receberam a terapêutica por 10 dias. As avaliações foram feitas na 1a e 4a sessões de tratamento. A presença e intensidade de dor espontânea, dor à palpação e máxima abertura bucal foram mensuradas. A evolução destas foi comparada à classificação dos pacientes de acordo com os parâmetros do eixo II: grau de dor crônica (GDC), depressão e sintomas físicos não específicos (SFNE). Os dados foram analisados usando os testes de Fisher, Wilcoxon, t de Student, Kruskal-Wallis, U de Mann-Whitney e análise de variâncias, adotando-se p<0,05 como nível de significância. Foi possível observar melhora significativa (p<0,05) dos pacientes estudados quanto ao tempo em relação à EVA e dor à palpação tanto muscular quanto articular. Notou-se diferença estatística significante (p<0,05) entre os grupos de GDC e abertura bucal 4ª sessão e dor articular; entre os grupos de SFNE (incluindo e excluindo itens de dor) e palpação muscular 1ª sessão. Entre os grupos de GDC e as variáveis de EVA e palpação muscular foi encontrada diferença marginalmente significativa, apresentando p-valores próximos a 0,05 (p<0,1). Não foi encontrada diferença estatística significante entre os grupos de depressão e as variáveis consideradas. Portanto, foi observada relação entre alta incapacidade e maiores médias dor articular, grau de SFNE incluindo e excluindo itens de dor classificados como severos e palpação muscular inicial. A depressão não apresentou relação com as variáveis estudadas. / The aim of this study was assess the influence of Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) Axis II parameters on treatment outcome of low-level laser therapy (LLLT) associated with piroxicam in patients with TMD. Thirty-two patients (mean age 33.2 years old ± 13.4) with temporomandibular joint (TMJ) arthralgia were enrolled in the study and received three kinds of treatment: LLLT + piroxicam (LPi), LLLT + placebo piroxicam (L) e piroxicam + placebo LLLT (Pi). Patients were managed for ten days. Follow-up evaluations were done at the 1st and 4th consults. The presence and intensity of spontaneous pain through the visual analogue scale (VAS), painful palpation and mandibular maximum vertical opening were measured. The therapeutic outcomes of this population were compared between the classifications of axis II scores: graded chronic pain (GCP), depression and non-specific physical symptoms (NEPS). The data was analyzed using the following statistic models: Fisher\'s test, Student t test, Wilcoxon, Mann-Whitney U test, Kruskal-Wallis test and Analysis of Variance. The statistical significance level set was p<0.05. The variables VAS, painful joint and muscle palpation showed improvement over time. Significant difference was found between GCP groups and maximum mouth opening on the 4th session and painful TMJ palpation; between NEFS (including and excluding pain items) and painful muscle palpation on the 1st consult. Between groups of GCP and VAS\'s variables it was found a marginally significant relation, showing p-values near 0.05 (p<0.1). No statistical difference was found on the comparison between depression and the physical variables considered. Thus, it is possible to conclude that there is an association between high incapacity and higher mean values to painful joint palpation, severe NEPS including and excluding pain items and baseline painful muscle palpation. No relation between depression and the studied variables was observed.

No description available.

Disfunções Temporomandibulares e Dor Orofacial ou DTM, referem-se a um conjunto de alterações funcionais e/ou estruturais que se manifestam nas articulações temporomandibulares (ATMs), nos músculos da mastigação e nas estruturas associadas que compõem o aparelho mastigatório (Okeson, 2013). A DTM tem o potencial de não só interferir nas atividades funcionais do sistema estomatognático, mas também de afetar o comportamento social e psicológico do indivíduo. Este trabalho avaliou a amplitude dos movimentos mandibulares e a dor a palpação muscular no pré e pós-tratamento de participantes com DTM. Os participantes incluídos na pesquisa responderam ao Research Diagnostic for Temporomandibular Dysfunction (RDC/TMD) para viabilizar a formulação do diagnóstico de DTM e qualificar a dor a palpação dos músculos faciais, e submetidos à análise dos movimentos através de uma análise 3D, o JMA que avalia os movimentos mandibulares por vias ultrassônicas, no pré e póstratamento com placa estabilizadora por 30 e 60 dias. Os participantes foram reavaliados, com o objetivo de saber se haverá uma melhora na amplitude dos movimentos mandibulares pela abertura, lateralidades e protrusão e da movimentação condilar dentro da cavidade articular no plano sagital e frontal. Os resultados demonstraram não ter diferenças estatísticas na maior parte dos movimentos excursivos mandibulares (abertura, lateralidade e protrusão) avaliados tanto pelo sistema de ultrassom JMA quanto pelo exame do RDC/TMD. Na análise descritiva dos resultados obtidos através da palpação dos músculos na aplicação do RDC/TMD observamos uma grande melhora no quadro clinico de dor a palpação após 60 dias de uso da Placa Oclusal estabilizadora. Também não observamos diferenças estatisticamente significantes entre os dados coletados de amplitude dos movimentos pelo sistema informatizado JMA e pelo questionário RDC/TMD, ou seja, podemos afirmar que a avaliação pelo JMA é precisa e confiável. / Temporomandibular Disorders and Orofacial Pain or TMD, refer to a set of functional and / or structural changes that are manifested in the temporomandibular joints (TMJ) in the masticatory muscles and associated structures that make up the masticatory system (Okeson, 2013). The TMD has the potential to not only interfere with the functional activities of the stomatognathic system, but also affect the social and psychological behavior of the individual. This study evaluated the range of mandibular movements and muscle palpation pain in the pre and post-treatment of participants with TMD. Participants included in the study responded to the Research Diagnostic for Temporomandibular Dysfunction (RDC / TMD) to allow the formulation of a diagnosis of TMD and qualify pain palpation of the facial muscles, and subjected to analysis of movement through 3D JMA assessing movements mandibular by ultrasonic means, before and after treatment with stabilizing plate for 30 and 60 days. Participants were reassessed in order to know if there will be an improvement in the range of mandibular movements for opening, laterality and protrusion and condyle movement within the joint cavity in the sagittal and frontal plane. The results showed no statistical differences have in most excursive mandibular movements (opening, lateral and protrusive) assessed both by JMA ultrasound system as the examination of the RDC / TMD. In the descriptive analysis of the results obtained by palpation of the muscles in the application of the RDC / TMD noticed a great improvement in the clinical picture of pain palpation after 60 days of use board stabilizing occlusal. We did not observe statistically significant differences between the data collected from the range of motion by the computerized system JMA and the questionnaire RDC / TMD, other words, we can say that the evaluation by the JMA is accurate and reliable.

O presente estudo avaliou a relação dos parâmetros do eixo II dos Critérios de Diagnóstico de Pesquisa das Disfunções Temporomandibulares (RDC/TMD) na resposta terapêutica de pacientes com DTM. Trinta e três pacientes (média de idade de 33,2 anos ± 13,4) com artralgia da articulação temporomandibular (ATM) foram submetidos a três terapêuticas: laser de baixa potência (LBP) + piroxicam (LPi), LBP + placebo de piroxicam (L) e piroxicam + placebo de LBP (Pi). Os pacientes receberam a terapêutica por 10 dias. As avaliações foram feitas na 1a e 4a sessões de tratamento. A presença e intensidade de dor espontânea, dor à palpação e máxima abertura bucal foram mensuradas. A evolução destas foi comparada à classificação dos pacientes de acordo com os parâmetros do eixo II: grau de dor crônica (GDC), depressão e sintomas físicos não específicos (SFNE). Os dados foram analisados usando os testes de Fisher, Wilcoxon, t de Student, Kruskal-Wallis, U de Mann-Whitney e análise de variâncias, adotando-se p<0,05 como nível de significância. Foi possível observar melhora significativa (p<0,05) dos pacientes estudados quanto ao tempo em relação à EVA e dor à palpação tanto muscular quanto articular. Notou-se diferença estatística significante (p<0,05) entre os grupos de GDC e abertura bucal 4ª sessão e dor articular; entre os grupos de SFNE (incluindo e excluindo itens de dor) e palpação muscular 1ª sessão. Entre os grupos de GDC e as variáveis de EVA e palpação muscular foi encontrada diferença marginalmente significativa, apresentando p-valores próximos a 0,05 (p<0,1). Não foi encontrada diferença estatística significante entre os grupos de depressão e as variáveis consideradas. Portanto, foi observada relação entre alta incapacidade e maiores médias dor articular, grau de SFNE incluindo e excluindo itens de dor classificados como severos e palpação muscular inicial. A depressão não apresentou relação com as variáveis estudadas. / The aim of this study was assess the influence of Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) Axis II parameters on treatment outcome of low-level laser therapy (LLLT) associated with piroxicam in patients with TMD. Thirty-two patients (mean age 33.2 years old ± 13.4) with temporomandibular joint (TMJ) arthralgia were enrolled in the study and received three kinds of treatment: LLLT + piroxicam (LPi), LLLT + placebo piroxicam (L) e piroxicam + placebo LLLT (Pi). Patients were managed for ten days. Follow-up evaluations were done at the 1st and 4th consults. The presence and intensity of spontaneous pain through the visual analogue scale (VAS), painful palpation and mandibular maximum vertical opening were measured. The therapeutic outcomes of this population were compared between the classifications of axis II scores: graded chronic pain (GCP), depression and non-specific physical symptoms (NEPS). The data was analyzed using the following statistic models: Fisher\'s test, Student t test, Wilcoxon, Mann-Whitney U test, Kruskal-Wallis test and Analysis of Variance. The statistical significance level set was p<0.05. The variables VAS, painful joint and muscle palpation showed improvement over time. Significant difference was found between GCP groups and maximum mouth opening on the 4th session and painful TMJ palpation; between NEFS (including and excluding pain items) and painful muscle palpation on the 1st consult. Between groups of GCP and VAS\'s variables it was found a marginally significant relation, showing p-values near 0.05 (p<0.1). No statistical difference was found on the comparison between depression and the physical variables considered. Thus, it is possible to conclude that there is an association between high incapacity and higher mean values to painful joint palpation, severe NEPS including and excluding pain items and baseline painful muscle palpation. No relation between depression and the studied variables was observed.

Submitted by isabela.moljf@hotmail.com (isabela.moljf@hotmail.com) on 2017-05-17T12:11:32Z No. of bitstreams: 1 glauciaarleudasilva.pdf: 3423860 bytes, checksum: 769aaed9184977e90b0c5f5446d55722 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-05-17T16:16:18Z (GMT) No. of bitstreams: 1 glauciaarleudasilva.pdf: 3423860 bytes, checksum: 769aaed9184977e90b0c5f5446d55722 (MD5) / Made available in DSpace on 2017-05-17T16:16:18Z (GMT). No. of bitstreams: 1 glauciaarleudasilva.pdf: 3423860 bytes, checksum: 769aaed9184977e90b0c5f5446d55722 (MD5) Previous issue date: 2010-07-30 / O objetivo deste trabalho foi avaliar a acurácia dos Critérios de Diagnóstico para Pesquisa das Desordens Temporomandibulares (RDC/DTM), a partir da comparação com o exame de imagem por ressonância magnética (IRM) no diagnóstico dos deslocamentos de disco da articulação temporomandibular em pacientes que buscaram tratamento para desordem temporomandibular. Vinte pacientes foram avaliados, totalizando 40 articulações examinadas. Para detectar anormalidades na posição do disco clinicamente foi empregado o uso do RDC/DTM, por um único examinador calibrado, “cego” para os exames de IRM. O diagnóstico das IRM obtidas dos pacientes foi realizadas por dois especialistas em radiologia odontológica, “cegos” para o exame clínico. Com base na estatística Kappa, foram observados níveis aceitáveis de confiabilidade intra-examinador na aplicação do RDC/DTM, e intra e interexaminador na avaliação das IRM. Os valores de kappa para a concordância entre o RDC/DTM e IMR foram melhores nos diagnósticos de deslocamento de disco do lado direito (k=0,913). Neste estudo, o RDC/DTM mostrou-se válido para diagnosticar deslocamentos de disco articular da ATM, com acurácia de 95% para a avaliação das ATM do lado direito e 75% do lado esquerdo, evidenciando a necessidade de um exame padronizado realizado por investigadores treinados na avaliação de pacientes com DTM. / The aim of this study was to evaluate the accuracy of the Research Diagnostic Criteria to Temporomandibular Disorders (RDC/TMD), from the comparison with magnetic resonance imaging (MRI) findings in the diagnosis of disc displacements of the temporomandibular joint in patients seeking treatment for temporomandibular disorders. Twenty patients were evaluated in a total of 40 joints examined. To detect abnormalities in disc position was employed clinically using the RDC/TMD, by a single calibrated examiner, “blinded” to the MRI scans. The diagnosis of MRI was obtained from patients performed by two specialists in oral radiology, “blinded” to the clinical examination. Based on the Kappa value, were found acceptable levels of reliability intra examiner in the application of RDC/TM, and intra e inter examiner in the evaluation of MRI. Kappa values for agreement between RDC/TMD and MRI were better in the diagnosis of disc displacement on the right side (k = 0.913). In this study, the RDC/TMD was valid for diagnosing disc displacement of the TMJ, with 95% accuracy for the evaluation of ATM on the right side and 75% of the left side, suggesting the need for a standardized examination conducted by trained investigators in the evaluation of patients with TMD.

lt;p align="justify"gt;The Health Service Residuals (HSR) are the ones generated in a facility related to human or animal health. They represent 1% of the total of solid residuals and the inadequate manipulation is a source of risk for human health and the environment. Small-sized municipal districts also suffer from the problem of residuals and the greatest challenge is serving the legislation. The aim of this work is evaluating the HSR management and proposing low cost measures for its implementation and operation. It was a qualiquantitative approach, through the application of checklist, semi-structure and structured interviews, and a photographic record. Analyzing the classification, segregation, conditioning, identification, storing and transportation steps, not all the institutions attended the legal requirements. The municipal district has assumed that the responsibility of external transportation and final disposition didn’t attend the required technical specifications. The legal responsibility is from the generator source. It was perceived the lack of knowledge from professionals about HSR and the limitations in the use of individual protective equipment. It can be concluded that it is possible to attend the RDC 306/04 legislation highlighting low cost measures, in the focus on integrated management of shared residuals and responsibilities, and that these proposed measures can be applicable to the reality of other small-sized municipal districts.lt;/pgt; / lt;p align="justify"gt;Os Resíduos de Serviços de Saúde – RSS são aqueles gerados em estabelecimento relacionados à saúde humana e animal e mesmo representando apenas 1% do total de resíduos sólidos no município, o seu manejo inadequado representa grande risco para o meio ambiente. Nesse particular, os municípios de pequeno porte, apesar de produzirem pequena quantidade de RSS, enfrentam sérias dificuldades para gerir esses resíduos, considerando-se as limitações técnicas e financeiras para atender às legislações vigentes. O objetivo deste trabalho foi avaliar a percepção da gestão de RSS na cidade de Inhapim, Estado de Minas Gerais, e propor medidas de baixo custo para a operação dos serviços em todas as suas etapas, conforme preconiza a Resolução Deliberativa Colegiada (RDC) 306/04. Para isso, foi realizado o diagnóstico de percepção qualiquantitativo dos RSS gerados nos estabelecimentos de saúde localizados no município. Foram aplicados questionários (entrevistas estruturadas e semi-estruturadas) a gestores e servidores dos estabelecimentos de saúde, além de visitas in loco a todos os estabelecimentos de saúde e registro fotográfico. Foi analisada a gestão dos RSS em todas as suas etapas (classificação, segregação, acondicionamento, identificação, armazenamento e transporte) e chegou-se à conclusão de que os estabelecimentos de saúde de Inhapim não atenderam às exigências preconizadas pela RDC 306/04. Apesarda responsabilidade legal pela gestão dos RSS ser do gerador, o município de Inhapim tem assumido a responsabilidade do transporte externo e disposição final desses resíduos, mesmo dos estabelecimentos privados. De forma geral, conclui-se que há um desconhecimento dos gestores e servidores dos estabelecimentos de saúde sobre RSS e limitações no uso de equipamentos de proteção individual. Acredita-se que é possível atender à legislação RDC 306/04, valorizando medidas de baixo custo, dentro da ótica da gestão integrada de resíduos e responsabilidades compartilhadas, e que podem ser aplicáveis à realidade de outros municípios de pequeno porte.lt;/pgt;