Master’s thesis deals with design of single cylinder engine which is technology demonstrator of four cylinder naturally aspirated spark ignition engine Mazda Skyactiv-G. Outset of this thesis deals with construction of modern naturally aspirated engines and with comparison of their parameters. Computational part consists of forces in crank mechanism. These calculations are later used in FEM analysis of connecting rod.

The thesis deals with the structural design of a steel connecting rod for the aircraft engine Walter Mikron III, which is currently still manufactured and developed by company PARMA-TECHNIK, s.r.o. The proposed connecting rod would replace the current duralumin connecting rod. The work also contains a strength analysis of the designed component, in which it was aimed to verify the stress on the connecting rod, based on which the fatigue safety factor was performed. This is crucial for dynamically stressed parts of internal combustion engines. Furthermore, was investigated the effect of the change of the connecting rod on the torsional vibration of the crank mechanism. For this purpose, was built a discrete torsion model. The output of the whole work is the drawing documentation of the new connecting rod, meeting the requirements for application in an aircraft engine.

Very often defects are present in rolled products. For wire rods, defects are very deleterious since the wire rods are generally used directly in various applications. For this reason, the market nowadays requires wire rods to be completely defect-free. Any wire with defects must be rejected as scrap which is very costly for the production mill. Thus, it is very important to study the formation and evolution of defects during wire rod rolling in order to better understand and minimize the problem, at the same time improving quality of the wire rods and reducing production costs. The present work is focused on the evolution of artificial defects during rolling. Longitudinal surface defects are studied during shape rolling of an AISI M2 high speed steel and a longitudinal central inner defect is studied in an AISI 304L austenitic stainless steel during ultra-high-speed wire rod rolling. Experimental studies are carried out by rolling short rods prepared with arteficial defects. The evolution of the defects is characterised and compared to numerical analyses. The comparison shows that surface defects generally reduce quicker in the experiments than predicted by the simulations whereas a good agreement is generally obtained for the central defect.

Rostliny rodu Echinacea jsou léčivé byliny stimulující imunitní systém. Používají se zvláště v boji proti chřipce a infekcím horních cest dýchacích. V této práci bylo analyzováno 7 komerčních výrobků obsahujících výtažky z rostliny Echinacea vysokoúčinnou kapalinovou chromatografií. Bylo provedeno kvantitativní stanovení derivátů kyseliny kávové, jež jsou látky zodpovědné za léčivé účinky rostliny. Zjištěné obsahy kyseliny chlorogenové, kávové, cichorové a echinakosidu byly porovnány v jednotlivých výrobcích. Dále byly připraveny extrakty z kořene, listů a květů třapatky nachové pomocí extrakce za zvýšeného tlaku extrakčního rozpouštědla při různých podmínkách, aby se z obsahu účinných látek v nich přítomných zjistilo, které extrakční podmínky jsou nejlepší. Z naměřených dat bylo zjištěno, že největší celková koncentrace obsahových látek je přítomna ve výrobku Imunit Echinaceové kapky firmy Simply You. Jako nejlepší extrakční podmínky se ukázaly teplota 80 °C v kombinaci s rozpouštědlem ve složení EtOH/voda = 40/60, a to jak při extrakci kořene, tak i listů a květů.

The aim of this study was focused on the study of conditions of growth of strains Lbc. paracasei subsp. paracasei CCDM 211, Lbc. paracasei CCDM 212, Lbc. paracasei subsp. paracasei CCDM 213 and Lbc. salivarius CCDM 216 in media with different amount of carbon-source (glucose, lactose and whey). Next part of the experiment was dealed with study of conditions of bacteria growth at stress conditions (lower pH). The purity od bacterial culture was verified with help of streaking. Purity DNA isolated from bacteria was tested using agarose gel electrophoresis, DNA concentration was estimated spectrophotometricaly. The presence of bacteria of genus Lactobacillus was proved using polymerase chain reaction (PCR) with genus specific primers.

In this work the functionalised magnetic particles were tested with streptavidin to selective DNA isolation. The method of selective DNA isolation was tested by using DNA probiotic strain Lactobacillus paracasei subsp. paracasei CCDM 211/06. A test was done on the biotinyl oligonucleotic particles, which was immobilised by containing streptavidin and it was used like a DNA probe for isolation complementary DNA chain by means of DNA/DNA hybridization. The primer R 5´ bio and the biotinyl denatured specific PCR product were tested for species Lb. paracasei as a DNA probe. These following experimental conditions were optimized for selective DNA isolation: temperature and time of hybridization, amount of DNA and the release of DNA from microspheres. Isolation of DNA was verified by PCR with specific generic primers. The specific generic PCR product was amplified in extent 250 bp, which was detected by using electrophoresis in agarose gel. This optimized method was successfully used in selective isolation of DNA Lactobacillus from a complementary sample of supplementary food (BIFI pangamin).

Microbial DNA was isolated from lysed cells of Lactobacillus genus in probiotic products. Reversible adsorption DNA on the surface of carboxyl coated nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) magnetic particles and silicagel coated manganase Perovskite nanoparticles. DNA was adsorbed on the surface of the particles in the presence of 16 % poly(ethylenglycol) (PEG 6000) and 2 M sodium chloride (NaCl) concentrations. The adsorbed DNA was released from particles by low ionic strength TE buffer (pH= 8.0). The quality of isolated DNA was checked by spectrofotometric measurement and PCR amplification. DNA samples isolated using magnetic particles and phenol extraction method (control method) were PCR-ready. The DNA isolated from lysed cells of probiotic products was quantificated in real-time qPCR.

The master’s thesis deals with the topic new isolation lines of 110 kV. The aim is to propose new insulator hangers. The first, theoretical part focuses on the development of insulators, cutting and possibilities of application. They are the different materials that are used for the production of insulators HV, advantages and disadvantages long rod and cap and pin insulators. There are also described insulator hangers. The theoretical work is to describe thefittings for overhead transmission lines, which are, used to construct a insulator hanger. The last part of the theoretical work is intended distribution towers for overhead lines HV. The practical part deals with the design of new insulator suspensions for specific lines of 110 kV. The results are drawings newly designed hangers and a list of materials (fittings) for individual towers and the total amount of material. The practical part is calculating the differences in the conductor sag using old and new insulators hangers.

This thesis deals with the design and assessment of footbridge for pedestrians and cyclists in the city of Hranice, Rybare district. The footbridge will serve as a crossing point over the river Bečva and will conect cycling trail with the street Rybarska. The content of this paper is to propose two different variants of structures. One of these options is selected based on their economic evaluation and further processed. Length of the bridge is 50m.

In this diploma thesis is completed the FEM analysis of connecting rod in combustion engine Briggs & Stratton of the garden lawnmower. Within the frame of computing in the FEM system ANSYS Workbench is solved static structural strein and stress analysis, buckling analysis a fatigue also. The boundary conditions for FEM computing come out from the kinematic and dynamic analysis of the crank-slider mechanism in combustion engine. The 3-D models of the crank-slider mechanism are created in parametric SolidWorks modeller. Own construction variants of the connecting rod based on results of FEM analysis are also designed.