At present there is no simple, yet scientifically robust method for calculating insurance loss estimates due to a fire. Therefore building owners and insurers can not make suitably informed decisions when selecting fire protection measures or setting premiums as they have no way of defining the true risk they face. As a consequence this research aims to investigate a number of techniques in an effort to define one as appropriate for further research. Three different methods were explored and consist of risk based analysis, deterministic hand calculations and Computational Fluid Dynamics (CFD). Extensive literature reviews were conducted in each area and the final models were based on the outcomes of this research. Rack storage warehouses were chosen for analysis as they are currently topical within the fire engineering community and are a particular concern for insurers. The risk based analysis employed statistical techniques including event tree analysis and monte carlo simulation to calculate loss distributions and sensitivity analyses. The hand calculation method was based on equations presented in the literature and incorporated the use of a zone model (BRANZFire) to calculate deterministic loss estimates. The CFD model used was Fire Dynamics Simulator and full scale warehouse fires were modelled using this programme. It was concluded that Fire Dynamics Simulator is an inappropriate tool as the capability for providing loss estimates in a timely manner is currently beyond the model's capabilities. Of the two remaining methods the statistical risk based model was selected as the most appropriate for further investigation. The primary reasons for this decision were the ability to calculate loss distributions and conduct sensitivity analyses, as well as its versatility and user friendliness. Improved statistical data was defined as imperative for future development of the model.

The thesis is focused on mapping issues when creating data networks. Overview solved problems briefly introduces the history and classification networks. Continues characteristics of active and passive parts of the network where it connects certification, ending a general guide for network architects, which can be used to understand the theory of progress in the design and implementation of data networks with regard to its functionality and reliability. This section explaining how the proposal looks like a LAN, which is one of the most widespread types of networks, and most standards it is just related. The practical part is focused on the demonstration of a possible case design, including its economic parameters. The various alternatives are worked out with regard to the choice of materials offered different levels of quality and possible types of treatment for the future. The alternatives are as follows economically analyzed and evaluated.

Programa de Pós Graduação em Engenharia Civil. Departamento de Engenharia Civil, Escola de Minas, Universidade Federal de Ouro Preto. / Submitted by giuliana silveira (giulianagphoto@gmail.com) on 2016-02-15T16:41:05Z No. of bitstreams: 1 DISSERTAÇÃO_EstudoTeóricoExperimentalSistemas.pdf: 2483971 bytes, checksum: f88c97824d253148d1fe18d72ddd039b (MD5) / Approved for entry into archive by Oliveira Flávia (flavia@sisbin.ufop.br) on 2016-02-15T18:47:44Z (GMT) No. of bitstreams: 1 DISSERTAÇÃO_EstudoTeóricoExperimentalSistemas.pdf: 2483971 bytes, checksum: f88c97824d253148d1fe18d72ddd039b (MD5) / Made available in DSpace on 2016-04-12T17:53:06Z (GMT). No. of bitstreams: 1 DISSERTAÇÃO_EstudoTeóricoExperimentalSistemas.pdf: 2483971 bytes, checksum: f88c97824d253148d1fe18d72ddd039b (MD5) Previous issue date: 2003 / Neste trabalho apresenta-se um estudo do comportamento de sistemas de armazenagem industrial do tipo Drive-in. Apesar de ser uma estrutura composta de perfis formados a frio, o seu projeto não pode ser baseado nas normas tradicionais de dimensionamento. Isto porque os sistemas de armazenagem industrial possuem como característica própria perfurações ao longo dos perfis que compõem as colunas e que são utilizados para facilitar o encaixe das vigas. Para o presente estudo foram utilizadas as prescrições de norma do RMI - Rack Manufacturers Institute com algumas modificações para abranger o sistema Drive-in Por ser uma estrutura esbelta e composta por perfis formados a frio, a estabilidade do sistema Drive-in é importante, sendo interessante conhecer a rigidez real das ligações. Neste trabalho foi avaliada experimentalmente a rigidez da ligação colunaplaca de base, enquanto os valores de rigidez das demais ligações foram obtidos em estudos anteriores. As análises foram feitas através de um software em elementos finitos, em que a estrutura foi modelada com elementos de viga e elementos de mola. Estudou-se o tipo de análise que melhor representa o comportamento da estrutura: linear ou não linear, bidimensional ou tridimensional; avaliou-se a perda de influência do plano de contraventamento dos fundos nas colunas frontais; validou-se a obtenção da área efetiva da coluna através da tensão nominal segundo a NBR-14672; estudou-se a importância da consideração dos dados experimentais no comportamento global da estrutura, aplicaram-se forças horizontais como forma de representar a estrutura fora do prumo e foram feitas algumas considerações sobre a determinação da carga crítica da coluna. Essas análises têm como objetivo conhecer o comportamento e propor recomendações para projeto de sistemas do tipo Drive-in. ___________________________________________________________________________________ / ABSTRACT: This work studies the behavior of the Drive-in, which is a steel storage rack system. Although these systems consist of thin cold-formed steel sections, their projects cannot be based on the traditional codes. It happens because there are holes en the columns of the racks. These holes are used to make the rabbet of the beams easier. In this study was used a specific code to steel storage racks systems of the RMI - Rack Manufacturers Institute with some modifications because this code doesn’t enclose the Drive-in system. Being a slender structure, the stability of the Drive-in system is important, and then in this case the knowledge of the true stiffness of the joining is important. At the present work the stiffness of de base plates was determined by test. The values of the others stiffness used in this study are the previous studies. A study about a better analysis – linear or non-linear and 2D or 3D – to represent the structure behavior was made by software in finite elements, where the structure was shaped with beam and spring elements. The influence of the bracing was evaluated in the front columns. The calculation of the column’s area through the NBR-14672’s stress was validated. The experimental data in the global behavior of the structure was considered important. Horizontal forces were applied as form to the effect of the out-ofplumbness in the structure and some considerations were made about the critical load of the column. These analyses have as objective to know the behavior and to consider recommendations for project of Drive-in system.

The content of this thesis is the design of street furniture using concrete. The work includes a brief summary of marketing, analyzing current trends in design of street furniture and analysis used technical solutions. The main part is devoted to the design process, resulting in the shape of street furniture while respecting the structural and ergonomic requirements in relation to people who use the furniture.

The content of this thesis is the design of street furniture using concrete. The work includes a brief summary of marketing, analyzing current trends in design of street furniture and analysis used technical solutions. The main part is devoted to the design process, resulting in the shape of street furniture while respecting the structural and ergonomic requirements in relation to people who use the furniture.

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Orientador: Jorg Kobarg / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-04T15:24:55Z (GMT). No. of bitstreams: 1 Nery_FlaviaCristina_D.pdf: 4972440 bytes, checksum: 86b749df91267d19e655449a4dfc3d0c (MD5) Previous issue date: 2005 / Resumo: Ki-1/57 é um antígeno humano de 57kDa reconhecido pelo anticorpo Ki-1, o qual também reconhece CD30. Ki-1/57 se encontra no núcleo e no citoplasma sendo fosforilado nos resíduos de serina e de treonina após a ativação das células. Quando Ki-1/57 foi isolado da linhagem L540 de células de linfoma de Hodgkin, ela co-imunoprecipitou com atividade quinase em resíduos de Ser/Thr. Além disso, foi relatado que Ki-1/57 interage com o ácido hialurônico e conseqüentemente foi denominada de ¿proteína intracelular que se liga a hialuronato 4¿ (IHABP4). Nós usamos o sistema de duplo híbrido em levedura e encontramos que Ki-1/57 interage especificamente com a proteína com domínios cromo-helicase e de ligação ao DNA 3 (CHD3), uma proteína nuclear envolvida em remodelagem da cromatina e regulação da transcrição, e com RACK1 (¿proteína adaptadora para quinase C ativada¿). A interação entre Ki-1/57 e seus ligantes foi confirmada por outros experimentos in vitro e in vivo. Interessantemente, a interação entre Ki-1/57 e RACK1 foi abolida após fosforilação da Ki-1/57 e observamos que o estímulo de células L540 e HeLa com 4 a -forbol 12-miristato 13-acetato (PMA) resulta na saída de Ki-1/57 do núcleo. Ki-1/57 também demonstrou ser um substrato para proteína quinase C (PKC) quando ativada com PMA, e sua fosforilação foi confirmada in vitro e in vivo. Esses dados sugerem que Ki-1/57 está associada com a via de sinalização celular de RACK1/PKC e isto pode ser importante para a regulação de suas funções nucleares. Sua interação com CHD3 e com outras proteínas envolvidas na regulação transcricional, tais como: Topors, Daxx e Tip60, entre outras, sugere que Ki-1/57 pode ter uma função neste contexto funcional. RACK1 interage com p73, um parálogo de p53, inibindo sua ativação transcricional. Nós ainda encontramos que Ki-1/57 também interage com p53 não fosforilada e pode inibir sua ativação transcricional. A estrutura tridimensional da proteína Ki-1/57 é desconhecida, mas nossos estudos espectroscópicos mostraram que a proteína Ki-1/57 é predominantemente constituída por folhas b-pregueadas. Além disso, Ki-1/57(122-413) apagou o sinal do espectro de CD de RACK1 entre 229-300 nm, que é característico de proteínas ricas em triptofanos, e também diminuiu a intensidade de emissão de fluorescência de RACK1. Isso sugere que Ki-1/57 interage com os triptofanos na superfície de RACK1 / Abstract: Ki-1/57, the 57-kDa human protein antigen recognized by the CD30 antibody Ki-1, is a cytoplasmic and nuclear protein, which is phosphorylated on serine and threonine residues upon cell activation. When isolated from the Hodgkin¿s lymphoma analogous cell line L540 Ki-1/57 was co-immunoprecipitated with a Thr/Ser protein kinase activity. It has been also found to interact with hyaluronic acid and has therefore been termed intracellular hyaluronan binding protein 4 (IHABP4). We used the yeast-two-hybrid system to identify proteins interacting with Ki-1/57 and found that Ki-1/57 engages in specific interactions with the Chromatin-Helicase-DNA-binding domain protein 3 (CHD3), a nuclear protein involved in chromatin remodeling and transcription regulation, and with the adaptor protein Receptor of Activated Kinase-1 (RACK1). Next, we confirmed these interactions by in vitro and in vivo experiments. Interestingly, the interaction of Ki-1/57 with RACK1 is abolished upon activation of L540 cells with 4a-phorbol 12-myristate 13-acetate (PMA), which results in the phosphorylation of Ki-1/57 and its exit from the nucleus. We demonstrated that Ki-1/57 also co-precipitates with protein kinase C (PKC) when isolated from PMA activated L540 tumor cells and is a substrate for PKC phosphorylation in vitro and in vivo. These events associate Ki-1/57 with the RACK1/PKC pathway and may be important for the regulation of its nuclear functions. Its interaction with chromatin remodeling factors such as CHD3 and other proteins involved in transcriptional regulation including Topors, Daxx and Tip 60 may suggest that Ki-1/57 has also a function in this context and that this function is subject to regulatory events involving the PKC/RACK1 signaling pathway. RACK1 also interacts with the p53 paralogue p73. In that case, the physical binding of RACK1 to p73 can inhibit its transcription activation function. We found out that Ki-1/57 interacts with unphosphorylated p53 and that this binding inhibits p53 transcription activation function. The three-dimensional structure of Ki-1/57 is still unknown but our spectroscopic studies demonstrated that Ki-1/57 consists predominantly of b-sheets. Binding of Ki-1/57(122-413) to RACK1 abolishes its positive ellipticity at 229-300 nm, which is characteristic for tryptophan-rich proteins, and decreases its emission fluorescence. This suggests that surface tryptophans of RACK1 are involved in the interaction with Ki-1/57 / Doutorado / Genetica Humana e Medica / Doutor em Genetica e Biologia Molecular

Improving the efficiency of conventional air-cooled solutions for Data Centers (DCs) is still a major thermal management challenge. Improvements can be made in two ways, through better (1) architectural design and (2) operation. There are three conventional DC cooling architectures: (a) room-based, (b) row-based, and (c) rack-based. Architectures (b) and (c) allows a modular DC design, where the ITE is within an enclosure containing a cooling unit. Due to scalability and ease of implementation, operational cost, and complexity, these modular systems have gained in popularity for many computing applications. However, the yet poor insight into their thermal management leads to limited strategies to scale the size of a DC facility for applications gaining in importance, e.g., edge and hyperscale. We improve the body of knowledge by comparing three cooling architecture’s power consumption. Energy efficiency during DC operation can be improved in two ways: (1) utilizing energy efficient control systems, (2) optimizing the arrangement of ITE. For both cases, a temperature prediction tool is required which can provide real-time information about the temperature distribution as a function of system parameters and the ITE arrangement. To construct such a prediction tool, we must develop a deeper understanding of the airflow, pressure and temperature distributions around the ITE and how these parameters change dynamically with IT load. As yet primitive tools have been developed, but only for architecture (a) listed above. These tools are not transferrable to other architectures due to significant differences in thermal-fluid transport. We examine the airflow and thermal transport within confined racks with separated cold and hot chambers that employ rack- or row-based cooling units, and then propose a parameter-free transient zonal model to obtain the real-time temperature distributions. / Thesis / Doctor of Philosophy (PhD)