La captación de carbono por la vegetación es a escala global el flujo más grande de CO2 e influencia en gran medida el funcionamiento de los ecosistemas. Sin embargo, su variabilidad temporal y espacial sigue siendo poco conocida y difícil de estimar. Las técnicas de teledetección pueden ayudar a calcular mejor la producción primaria bruta (GPP) terrestre, que es la expresión a nivel de ecosistemas del proceso de la fotosíntesis. El objetivo principal de esta tesis fue encontrar una manera de estimar la variabilidad espacial y temporal de la eficiencia en el uso de la radiación (RUE) a escala de ecosistema y por lo tanto mejorar la estimación de la GPP de la vegetación terrestre por medio de datos de teledetección. Se abordaron cuatro objetivos específicos. El primero fue analizar y sintetizar la literatura científica sobre la relación entre el Índice de Reflectancia Fotoquímica (PRI), un índice espectral vinculado a la eficiencia fotosintética, y diversas variables ecofisiológicas a través de un amplio rango de tipos funcionales de plantas y ecosistemas. El segundo objetivo fue analizar y sintetizar los datos de la variabilidad espacial de la GPP y la variabilidad espacial y temporal de la RUE y sus controles climáticos para un amplio rango de tipos de vegetación, desde la tundra a la selva tropical. El tercer objetivo fue comprobar si diferentes índices espectrales, es decir, el PRI, el NDVI (Normalized Difference Vegetation Index) y EVI (Enhanced Vegetation Index), derivados del Moderate Resolution Imaging Spectroradiometer (MODIS) son buenos estimadores de la captación de carbono a diferentes escalas temporales en un bosque mediterráneo. El cuarto objetivo fue evaluar el uso de MODIS PRI como estimador de la RUE en un amplio rango de tipos de vegetación mediante el uso de datos sobre la captación de carbono de la vegetación derivados de las torres de covarianza turbulenta.Las principales conclusiones de esta tesis son que hay una coherencia emergente de la relación RUE-PRI que sugiere un sorprendente grado de convergencia funcional de los componentes bioquímicos, fisiológicos y estructurales que afectan la eficiencia de captación de carbono a escala de hoja, de cobertura y de ecosistemas. Al complementar las estimaciones de la fracción de radiación fotosintéticamente activa interceptada por la vegetación (FPAR), el PRI permite mejorar la evaluación de los flujos de carbono a diferentes escalas, a través de la estimación de la RUE. Una segunda conclusión apoya la idea de que el funcionamiento anual de la vegetación es más limitado por la disponibilidad de agua que por la temperatura. La variabilidad espacial de la RUE anual y máxima puede explicarse en gran medida por la precipitación anual, más que por el tipo de vegetación. Una tercera conclusión es que, si bien EVI puede estimar el incremento diametral anual de los troncos, y el PRI puede estimar la fotosíntesis neta diaria nivel de hoja y la eficiencia en el uso de radiación, el papel del NDVI es más limitado como un estimador de cualquier parte del ciclo del carbono en bosques mediterráneos. Por lo tanto, el EVI y el PRI son excelentes herramientas para el seguimiento del ciclo del carbono en los bosques mediterráneos. Por último, el PRI derivado de información satelital disponible libremente, presenta una relación positiva significativa con la RUE para un amplio rango de diferentes tipos de bosques, incluso en años determinados, en bosques caducifolios. En general, esta tesis proporciona un mejor entendimiento de los controles espacial y temporal de la RUE y abre la posibilidad de estimar RUE en tiempo real y, por tanto, la captación de carbono de los bosques a nivel de ecosistemas a partir del PRI. / Carbon uptake by vegetation is the largest global CO2 flux and greatly influences the ecosystem functions. However, its temporal and spatial variability is still not well known and difficult to estimate. Remote sensing techniques can help to better estimate the terrestrial gross primary production (GPP), that is the ecosystem level expression of the photosynthesis process or the rate at which the ecosystem's producers capture CO2. The main objective of this thesis was to find a way to estimate the spatial and temporal variability of the Radiation Use Efficiency (RUE) at the ecosystem scale and therefore to arrive to more accurate ways to estimate GPP of terrestrial vegetation by means of remotely sensed data. Four specific objectives were addressed in this thesis. The first objective was to examine and synthesize the scientific literature on the relationships between the Photochemical Reflectance Index (PRI), a narrow-band spectral index linked to photosynthetic efficiency, and several ecophysiological variables across a wide range of plant functional types and ecosystems. The second objective was to analyze and synthesize data for the spatial variability of GPP and the spatial and temporal variability of the RUE and its climatic controls for a wide range of vegetation types, from tundra to rain forest. The third objective was to test whether different spectral indices, i.e. PRI, NDVI (Normalized Difference Vegetation Index) and EVI (Enhanced Vegetation Index), derived from the MODerate resolution Imaging Spectroradiometer (MODIS) can be indicators of carbon uptake at different temporal scales by analyzing the relationships between detailed ecophysiological variables at the stand level in a Mediterranean forest. The fourth objective was to assess the use of MODIS PRI as surrogate of RUE in a wide range of vegetation types by using data on carbon uptake of the vegetation derived from eddy covariance towers. The main conclusions of this thesis are that there is an emerging consistency of the RUE-PRI relationship that suggests a surprising degree of functional convergence of biochemical, physiological and structural components affecting leaf, canopy and ecosystem carbon uptake efficiencies. By complementing the estimations of the fraction of photosynthetically active radiation intercepted by the vegetation (fPAR) PRI enables improved assessment of carbon fluxes at different scales, through the estimation of RUE. A second conclusion supports the idea that the annual functioning of vegetation is more constrained by water availability than by temperature. The spatial variability of annual and maximum RUE can be largely explained by annual precipitation, more than by vegetation type. A third conclusion is that while EVI can estimate annual diametric wood increment, and PRI can estimate daily leaf level net photosynthesis and radiation use efficiency, the role NDVI is more limited as a surrogate of any part of the carbon cycle in this type of forest. Therefore, EVI and PRI are excellent tools for vegetation monitoring of carbon cycle in the Mediterranean forests, the first ones we tested in this thesis. Finally, the PRI derived from freely available satellite information was also found to present significant positive relationship with the RUE for a very wide range of different forest types, even in determined years, the deciduous forests. Overall, this thesis provides a better understanding of the spatial and temporal controls of the RUE and opens the possibility to estimate RUE in real time and, therefore, actual carbon uptake of forests at the ecosystem level using the PRI.Keywords carbon cycle, Normalized Difference Vegetation Index, Enhanced Vegetation Index, Photochemical Reflectance Index, primary productivity, photosynthesis, remote sensing, climatic controls, eddy covariance, radiation use efficiency, terrestrial vegetation.

The introduction of dwarfing genes in wheat and rice enabled significant yield improvements and was later termed the “Green-Revolution”. Dwarfing genes in sorghum have not been accompanied by such increases in grain production. On the contrary, some of the commercially employed dwarfing genes in sorghum have been associated with negative effects on grain yield. A positive correlation between plant height and grain yield was also observed in trial data for a diverse range of hybrids tested within the Queensland Department of Primary Industries and Fisheries Sorghum Breeding Program in north-eastern Australia. No attempts have previously been made to examine the physiological basis of the relationship between plant height and grain yield in sorghum. The dwarfing genes that are commercially used in wheat, Rht-B1 and Rht-D1 (formerly known as Rht1 and Rht2, respectively), on the other hand, have been studied extensively. They have been shown to have substantial and positive effects on grain number and harvest index, while not considerably reducing plant biomass, increasing grain yield. Our objective in this study was to examine the effect of height on the physiological and genetic determinants of growth and yield in sorghum to determine whether there was scope to improve yield by increasing the height of sorghum. A positive correlation between plant height and yield was observed in a population that was fixed for the major dwarfing genes, but showed variation in peduncle and panicle length, which are under control of minor dwarfing genes. To study the effects of a single major dwarfing gene (dw3) on biomass production and grain yield, 2- and 3-dwarf isogenic contrasts were developed in three different genetic backgrounds (R931945-2-2, R955343-1, R955637). In some cases, dw3 led to a significant reduction in plant biomass, which was not sufficiently offset by increase in harvest index to avoid yield reduction. This is contrary to the situation in wheat. The observed reductions in plant biomass in sorghum were associated with reduced tiller number and a reduction in radiation use efficiency (RUE) in the short types. Subsequent experiments suggested that an increase in allocation of biomass to the roots, rather than differences in photosynthetic capacity or respiration, was the main cause for the apparent reduction in RUE. However, due to plant-to-plant variability and the difficulty in accurately measuring root-total biomass ratio, studies with greater replication are required to confirm this hypothesis. It was also found that interactions with genetic background (and environment) moderated the effects of dw3, resulting in smaller height, biomass and grain yield reductions in some isogenic pairs. The effects of dwarfing genes on grain yield therefore need to be assessed separately for different genetic backgrounds. As lodging may be controlled by means other than height reduction (e.g. stay-green), we suggest that yield of standard sorghum types used in industrialised countries may benefit from moderate increases in plant height.

This research examined the mechanisms by which temperature, water availability and nitrogen (N) affect the dry matter (DM) yield potential of cocksfoot (Dactylis glomerata L.) dominant pastures. The experiment was a split plot design with main plots of fully irrigated (I) or dryland (D), sub-plots of N fertiliser at 800 kg N/ha in 2003/04; and 1600 kg N/ha in 2004/05 (+N) or 0 kg N/ha (-N). The potential environmental yield of an established 8 year old cocksfoot dominant pasture was 21.9 t DM/ha/y from I+N pastures compared with 9.8 t DM/ha by I-N pastures and 15.1 t DM/ha/y by D+N pastures. The lowest yields were from dryland pastures with no N which produced 7.5 t DM/ha/y in 2003/03 and 5.0 t DM/ha/y in 2004/05. The effect of seasonal temperatures on the DM production, when periods of water stress were excluded, was quantified using thermal time accumulated above a base temperature of 3°C as 7.0 kg DM/°Cd/ha for N fertilised pastures and 3.3 kg DM/°Cd/ha for pastures with no N. The 2.5 t DM/ha difference in yields of D-N pastures in 2003/04 and 2004/05 was the result of the duration, extent and timing of the water stress period. In both years the critical limiting deficit (DL) was calculated as 78 mm from the soil moisture deficit in the 0-0.8 m soil layers. Beyond DL yield decreased at a rate of 1.45%/mm in +N and –N pastures, relative to fully irrigated control pastures. Yields of D+N and D-N pastures were similar during periods of water stress with 0.4±0.1 t/DM/ha produced during the rotation ending 30/12/2003. This was less than from either the I-N (1.2 t DM/ha) or I+N (3.5 t DM/ha) pastures due to the reduction in the amount of photosynthetically active radiation intercepted by the canopies of the dryland pastures. However, in the rotation ending 2/5/2004, after autumn rain alleviated drought conditions, yield of the D+N pasture was 2.1 t DM/ha compared with 1.7 t DM/ha by I+N pastures. The effect of N on yield was described using a nutrition index which showed that as DM yield increased N% in the herbage declined. This is a function of the ratio between metabolic and structural N requirements rather than caused by ontogeny alone. Specific leaf N was determined at two harvests and appeared constant at a given point in time (1.0-1.6 g N/m² leaf). In contrast, specific pseudostem N increased from 0.8-1.0 g N/m² pseudostem at an NNI of 0.4 in –N pastures to 2.6-3.0 g N/m² pseudostem at an NNI of 1.2 in the +N pastures. Differences between the yields of +N and –N pastures were caused by differences in radiation use efficiency (RUE) as determined by the linear relationship (R²=0.76) between RUE and the nitrogen nutrition index (NNI). In this thesis, empirical relationships for the effects of temperature, water availability and N were derived and the physiological mechanisms which underlie these descriptions were identified. These relationships provide clear and simple explanations of the effects of environmental variables on the productivity of cocksfoot based pastures which will enhance understanding of the benefits and limitations of cocksfoot, particularly in dryland farming systems.

Made available in DSpace on 2014-06-11T19:24:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-02-01Bitstream added on 2014-06-13T18:52:30Z : No. of bitstreams: 1 assuncao_ica_me_botfca.pdf: 943075 bytes, checksum: ce539ad58264cbbab2e1e863aae2d636 (MD5) / Universidade Estadual Paulista (UNESP) / Um ensaio, com amendoim (Arachis hypogaea L.), cv. IAC-TATUST, foi conduzido, na área experimental do Setor de Ciências Ambientais (21,85° S; 48,43° W; 786 m), FCA/UNESP, em Botucatu, SP, em parcela única sob tratamento úmido durante o período de 11/02/2001 a 06/06/2001. Durante todas as fases de crescimento das plantas foram monitoradas as radiações fotossinteticamente ativa incidente, refletida e transmitida, bem como a produção semanal da biomassa acumulada e sua energia química, incluindo raízes, hastes, folhas, flores, frutos e sementes, com o objetivo de determinar a eficiência de uso da radiação (EUR) e a eficiência de conversão da radiação interceptada (ECR) pelas plantas cultivadas. Os resultados mostraram que a EUR média foi de 1,33 g MJ-1 e a ECR foi de 2,5%, cujos valores estão coerentes com aqueles encontrados em literatura. Nas condições de Botucatu, a cultura do amendoim interceptou, aproximadamente 600 MJ m-2 de radiação PAR, de modo que no final do seu ciclo a energia da biomassa atingiu a magnitude de 18 MJ m-2. Em termos médios, o coeficiente de extinção da luz (k) foi de 0,68, o índice de colheita, em base de energia, foi de 0,38 e a produtividade obtida, do amendoim em casca, foi equivalente a 4,5 t ha-£. / A trial, with peanut (Arachis hypogaea L.), cv. IAC-TATU-ST, was carried out, in experimental area of Setor de Ciências Ambientais (21,85° S; 48,43° W; 786 m), FCA/UNESP, in Botucatu, SP, in single section with humid treatment during the period from 11/02/2001 to 06/06/2001. For all growth phases of peanut crop, the incident, reflected and transmitted photosynthetically active radiations were monitored, as well as the weekly production of the accumulated biomass, chemical energy, including roots, stems, leaves, flowers, fruits and seeds, with purpose of determining the radiation use efficiency and the radiation conversion efficiency of intercepted PAR for the cultivated plants. The results showed that EUR was about 1,33 g MJ-1 and ECR was about 2,5%. The values are coherent with those found in literature. In Botucatu conditions, the peanut crop intercepted, more or less 600 MJ m-2 of PAR, so that in the end of cycle, the biomass energy reached to 18 MJ m-2. In standard terms, the light extinction coefficient (k) was about 0.68, the harvest index, in energy basis, was about 0.38 and peanut yield, in peel, was equivalent to 4.5 t ha-£.

Orientador: Dinival Martins / Banca: Raimundo Leite Cruz / Banca: Emerson Galvani / Resumo: Um ensaio, com amendoim (Arachis hypogaea L.), cv. IAC-TATUST, foi conduzido, na área experimental do Setor de Ciências Ambientais (21,85° S; 48,43° W; 786 m), FCA/UNESP, em Botucatu, SP, em parcela única sob "tratamento úmido" durante o período de 11/02/2001 a 06/06/2001. Durante todas as fases de crescimento das plantas foram monitoradas as radiações fotossinteticamente ativa incidente, refletida e transmitida, bem como a produção semanal da biomassa acumulada e sua energia química, incluindo raízes, hastes, folhas, flores, frutos e sementes, com o objetivo de determinar a eficiência de uso da radiação (EUR) e a eficiência de conversão da radiação interceptada (ECR) pelas plantas cultivadas. Os resultados mostraram que a EUR média foi de 1,33 g MJ-1 e a ECR foi de 2,5%, cujos valores estão coerentes com aqueles encontrados em literatura. Nas condições de Botucatu, a cultura do amendoim interceptou, aproximadamente 600 MJ m-2 de radiação PAR, de modo que no final do seu ciclo a energia da biomassa atingiu a magnitude de 18 MJ m-2. Em termos médios, o coeficiente de extinção da luz (k) foi de 0,68, o índice de colheita, em base de energia, foi de 0,38 e a produtividade obtida, do amendoim em casca, foi equivalente a 4,5 t ha-£. / Abstract: A trial, with peanut (Arachis hypogaea L.), cv. IAC-TATU-ST, was carried out, in experimental area of Setor de Ciências Ambientais (21,85° S; 48,43° W; 786 m), FCA/UNESP, in Botucatu, SP, in single section with "humid treatment" during the period from 11/02/2001 to 06/06/2001. For all growth phases of peanut crop, the incident, reflected and transmitted photosynthetically active radiations were monitored, as well as the weekly production of the accumulated biomass, chemical energy, including roots, stems, leaves, flowers, fruits and seeds, with purpose of determining the radiation use efficiency and the radiation conversion efficiency of intercepted PAR for the cultivated plants. The results showed that EUR was about 1,33 g MJ-1 and ECR was about 2,5%. The values are coherent with those found in literature. In Botucatu conditions, the peanut crop intercepted, more or less 600 MJ m-2 of PAR, so that in the end of cycle, the biomass energy reached to 18 MJ m-2. In standard terms, the light extinction coefficient (k) was about 0.68, the harvest index, in energy basis, was about 0.38 and peanut yield, in peel, was equivalent to 4.5 t ha-£. / Mestre

The literature suggests that grain number largely determines and as such limits yield in barley. Many of the reported studies were conducted in relatively low yielding environments and it is unclear if grain number is also a limiting factor in high yield potential climates. Nor is it known with certainty what physiological or morphological traits must be targeted in order to increase grain number. There may be a degree of trade-off between yield components whereby grain number is adjusted according to resource availability to the plant, either pre- or post-anthesis, in a way that ensures consistently well-filled grains at harvest. If mechanisms exist for adjusting grain numbers or grain storage capacity after anthesis to match assimilate availability, this may place limits on how far yield can be increased without increasing post-anthesis assimilate production. In order to determine the scope for increasing the yield potential of barley a more thorough understanding of the potential trade-offs between grain number, grain storage capacity and post-anthesis assimilate supply is required. The aim of research reported in this thesis was to establish what determines the yield of spring barley in Ireland and to investigate the timing and possible mechanisms involved in regulating grain number and grain storage capacity in relation to the supply of photoassimilates. Field experiments were carried out on spring barley (Hordeum vulgare L., cv. Quench) at several locations in Ireland from 2011 to 2013. A sub-set of experiments involving destructive sampling and in-field assessments on plots managed as per current best farm practice gathered crop growth, development, and yield component data across sites and seasons in order to establish what determines yield under typical crop production conditions. Separate experiments artificially manipulated the source:sink ratio of plots via shading and seed rate treatments to investigate in more detail the mechanisms determining grain number and grain weight and any potential trade-off between the two components. Grain number accounted for most of the variation in yield across 9 site/seasons of crops managed as per current best practice in Ireland (P < 0.001; R2 = 0.84) while grain weight remained relatively conserved. Ear number accounted for most of the variation in grain number (P = 0.002; R2 = 0.75) and ear number itself was largely determined by shoot survival from an early season peak through to harvest (P <0.001; R = 0.96). Shoot size and weight at the beginning of stem extension had the largest influence on shoot survival. Shading treatments were used to test whether there was a mechanism for adjusting grain numbers after anthesis to match the availability of assimilate for grain filling. Substantial post-anthesis reductions in assimilate supply during grain filling in 2011 and 2012 did not significantly reduce grain number (P > 0.05). A small reduction in grain number (8%) was found in response to shading for a two week period early post-anthesis in 2013, however this was likely a reduction in grain set in shoots or spikelets that reached anthesis after the treatment was imposed rather than a post-anthesis abortion or down-regulation of grain number. Percentage light interception by well managed (unshaded) canopies shortly after anthesis was generally greater than 93% across several sites and seasons, therefore increasing grain numbers to increase sink capacity would likely be associated with an unavoidable decrease in the amount of light intercepted per grain during the early grain development period. However, experiments showed that grain weight at harvest was neither reduced nor increased in response to variations in light interception during this period of endosperm development (P > 0.05), because soluble sugar concentrations in the grain were maintained at the expense of storage reserve deposition in the stems. Results suggest that grain number and grain storage capacity may both be determined pre-anthesis resulting in a trade-off during stem extension whereby grain numbers are adjusted in a way that helps conserve grain weight. A strong negative relationship between ear number and grain number per ear (P < 0.001; R2 = 0.81) across two sites of seed rate experiments in 2013 resulted in a plateau in overall grain number of approximately 18,000 grains m-2 suggesting that there may be a limit to how many grains can be established in a given environment; this was achieved with an ear number of approximately 1000 ears m-2. Yield potential for Irish conditions was estimated at 12.29 t ha-1 at 85% dry matter based on estimates of potential assimilate supply during grain filling; with a grain number of 26,481 m-2 required to utilise this. These estimates are both 44% higher than the mean yield and grain number achieved in crops managed as per current best farm practice. Once high potential ear numbers are secured (> 1000 m-2), breaking the negative relationship between ear number and grain number per ear may hold the key to further increasing grain number and hence yield potential. Increasing assimilate production and partitioning to ears during stem extension, either through increases in the duration of stem extension or solar radiation use efficiency, may enable larger grain numbers to be produced whilst maintaining or increasing individual grain storage capacity and deposition of stem storage reserves. Water and nutrient availability, as well as susceptibility to lodging may present further limitations to yield in the future.

Master of Science / Department of Agronomy / Robert M. Aiken / Increasing crop water use efficiency (WUE), the amount of biomass produced per unit water consumed, can enhance crop productivity and yield potential. The objective of the first study was to evaluate the factors affecting water productivity among eight sorghum (Sorghum bicolor (L.) Moench) genotypes, which differ in canopy architecture. Sorghum genotypes, grown under field conditions, showed significant differences in (a) biomass production, (b) water use, (c) intercepted radiation, (d) water productivity and (e) radiation use efficiency (RUE; the amount of biomass produced per unit of intercepted radiation which is suitable for photosynthesis). WUE and RUE were more strongly correlated to biomass production than to water use or intercepted radiation, respectively. RUE was positively correlated to WUE and tended to increase with internode length, the parameter used to characterize canopy architecture. These results demonstrate that increased utilization of radiation can increase water productivity in plants. Sorghum canopies that increase light transmission to mid−canopy leaves can increase RUE and also have the potential to increase crop productivity and WUE. The objective of the second study was to develop a quantitative model to predict leaf area index (LAI), a common quantification of canopy architecture, for sorghum from emergence to flag leaf stage. LAI was calculated from an algorithm developed to consider area of mature leaves (leaves with a ligule/collar), area of expanding leaves (leaves without a ligule/collar), total leaf area per plant and plant population. Slope of regression of modeled LAI on observed LAI varied for photoperiod sensitive (PPS) and insensitive (non−PPS) genotypes in 2010. A good correlation was found between the modeled and observed LAI with coefficient of determination (R[superscript]2) 0.96 in 2009 and 0.94 (non−PPS) and 0.88 (PPS) in 2010. These studies suggest that canopy architecture has prominent influence on water productivity of crops and quantification of canopy architecture through an LAI simulation model has potential in understanding RUE, WUE and crop productivity.

<p>In the scope of future population support, agricultural productivity, in particular in sub-Saharan Africa, has to increase drastically to meet the UN’s millennium development goals of eradicating extreme poverty and hunger by 2015. Water availability in the root-zone limits crop production in large parts of the developing world. As competition for fresh water increases, water of lower quality, for example saline or polluted water, is often used for irrigation. Low-cost drip systems are suitable for saline water irrigation because they effectuate a minimisation of salt accumulation, leaf burn and peaks in salt concentration. Nonetheless, all types of saline water irrigation contain the risk for causing soil salinisation. Thus, in order to achieve long-term sustainability of these systems, appropriate management strategies are needed. The choice of management practices may be influenced by local conditions such as climate, soil and irrigation water salinity. A litera-ture review showed that there is a potential for saline water irrigation in sub-Saharan Africa in water scarce areas. Low-cost drip irrigation with saline water (6 dS m-1) was successfully used to irrigate two consecutive crops of tomato in semi-arid South Africa. An integrated ecosystems model was developed to simulate long-term yield and salt accumulation in a drip-irrigated agricultural system for a range of salinities, climates and management techniques. Crop, salt and water balance data from two field experiments conducted in Israel and South Africa, respectively, were used to parameterise and test the model. Emphasis was placed on testing the usability of the model as a tool for evaluating the importance of certain plausible management options of low-cost, drip-irrigation systems. Therefore, particular focus was directed towards correctly describing soil salinity stress on plant growth and soil evaporation from a distributed (wetted and dry) surface. In addition, the model was developed to function for different climates without having to change any other parameters or variables except for the actual climatic data. Simulations were subsequently run over a 30-year period to study long-term yield and salt accumulation in the soil profile for two sites in South Africa, demonstrating the applicability of the model. Model simulations showed that high soil salinities reduced crop growth and thus increased both drainage and soil evaporation. Further, covering the soil with a plastic sheet led to a reduction of soil evaporation and a subsequent increase in both transpiration and drainage. Rainfall was crucial for the leaching of salts from the soil, and thus in regions with low levels of rainfall, a higher leaching fraction of supplied saline irrigation water has to compensate for the lack of rain. However, a high leaching fraction also causes large amounts of salt leaching, which could potentially pollute underlying groundwater and downstream ecosystems. This risk can be mitigated using mulching, which minimises non-productive water losses, thereby lowering irrigation water needs. The choice of irrigation water salinity, frequency of irrigation and soil coverage may differ between the farmer and the regional water manager due to different preferences. Furthermore, the study highlighted how environmental variables such as water use efficiency and radiation use efficiency can be used as indicators of system performance. Whereas the latter is first and foremost a general stress indicator, water use efficiency more precisely describes specific factors such as plant size, allocation patterns and evaporative demand, which will affect the exchange of carbon dioxide and water through the stomata.</p>

In the scope of future population support, agricultural productivity, in particular in sub-Saharan Africa, has to increase drastically to meet the UN’s millennium development goals of eradicating extreme poverty and hunger by 2015. Water availability in the root-zone limits crop production in large parts of the developing world. As competition for fresh water increases, water of lower quality, for example saline or polluted water, is often used for irrigation. Low-cost drip systems are suitable for saline water irrigation because they effectuate a minimisation of salt accumulation, leaf burn and peaks in salt concentration. Nonetheless, all types of saline water irrigation contain the risk for causing soil salinisation. Thus, in order to achieve long-term sustainability of these systems, appropriate management strategies are needed. The choice of management practices may be influenced by local conditions such as climate, soil and irrigation water salinity. A litera-ture review showed that there is a potential for saline water irrigation in sub-Saharan Africa in water scarce areas. Low-cost drip irrigation with saline water (6 dS m-1) was successfully used to irrigate two consecutive crops of tomato in semi-arid South Africa. An integrated ecosystems model was developed to simulate long-term yield and salt accumulation in a drip-irrigated agricultural system for a range of salinities, climates and management techniques. Crop, salt and water balance data from two field experiments conducted in Israel and South Africa, respectively, were used to parameterise and test the model. Emphasis was placed on testing the usability of the model as a tool for evaluating the importance of certain plausible management options of low-cost, drip-irrigation systems. Therefore, particular focus was directed towards correctly describing soil salinity stress on plant growth and soil evaporation from a distributed (wetted and dry) surface. In addition, the model was developed to function for different climates without having to change any other parameters or variables except for the actual climatic data. Simulations were subsequently run over a 30-year period to study long-term yield and salt accumulation in the soil profile for two sites in South Africa, demonstrating the applicability of the model. Model simulations showed that high soil salinities reduced crop growth and thus increased both drainage and soil evaporation. Further, covering the soil with a plastic sheet led to a reduction of soil evaporation and a subsequent increase in both transpiration and drainage. Rainfall was crucial for the leaching of salts from the soil, and thus in regions with low levels of rainfall, a higher leaching fraction of supplied saline irrigation water has to compensate for the lack of rain. However, a high leaching fraction also causes large amounts of salt leaching, which could potentially pollute underlying groundwater and downstream ecosystems. This risk can be mitigated using mulching, which minimises non-productive water losses, thereby lowering irrigation water needs. The choice of irrigation water salinity, frequency of irrigation and soil coverage may differ between the farmer and the regional water manager due to different preferences. Furthermore, the study highlighted how environmental variables such as water use efficiency and radiation use efficiency can be used as indicators of system performance. Whereas the latter is first and foremost a general stress indicator, water use efficiency more precisely describes specific factors such as plant size, allocation patterns and evaporative demand, which will affect the exchange of carbon dioxide and water through the stomata. / QC 20101102

<p>Dissecting the soybean grain yield (GY) to approach it as a sum of its associated processes seems a viable approach to explore this trait considering its complex multigenic nature. Monteith (1972, 1977) first defined potential yield as the result of three physiological efficiencies: light interception (Ei), radiation use efficiency (RUE) and harvest index (HI). Though this rationality is not recent, few works assessing these three efficiencies as strategies to improve crops have been carried out. This thesis approaches yield from the perspective of Ei, RUE, and HI to better understand yield as the result of genetic and physiological processes. This study reveals the phenotypic variation, heritability, genetic architecture, and genetic relationships for Ei, RUE, and HI and their relationships with GY and other physiological and phenological variables. Similarly, genomic prediction is presented as a viable strategy to partially overcome the tedious phenotyping of these traits. A large panel of 383 soybean recombinant inbred lines (RIL) with significant yield variation but shrinkage maturity was evaluated in three field environments. Ground measurements of dry matter, photosynthesis (A), transpiration (E), water use efficiency (WUE), stomatal conductance (gs), leaf area index (LAI) and phenology (R1, R5, R8) were measured. Likewise, RGB imagery from an unmanned aircraft system (UAS) were collected with high frequency (~12 days) to estimate the canopy dynamic through the canopy coverage (CC). Light interception was modeled through a logistic curve using CC as a proxy and later compared with the seasonal cumulative solar radiation collected from weather stations to calculate Ei. The total above ground biomass collected during the growing season and its respective cumulative light intercepted were used to derive RUE through linear models fitting, while apparent HI was calculated through the ratio seeds dry matter vs total above-ground dry matter. Additive-genetic correlations, genome wide association (GWA) and whole genome regressions (WGR) were performed to determine the relationship between traits, their association with genomic regions, and the feasibility of predicting these efficiencies through genomic information. Our results revealed moderate to high phenotypic variation for Ei, RUE, and HI. Additive-genetic correlation showed a strong relationship of GY with HI and moderate with RUE and Ei when the whole data set was considered, but negligible contribution of HI on GY when just the top 100 yielding RILs were analyzed. High genetic correlation to grain yield (GY) was also observed for A (0.87) and E (0.67), suggesting increase in GY can be achieved through the improvement of A or E. The GWA analyses showed that Ei is associated with three SNPs; two of them located on chromosome 7 and one on chromosome 11 with no previous quantitative trait loci (QTLs) reported for these regions. RUE is associated with four SNPs on chromosomes 1, 7, 11, and 18. Some of these QTLs are novel, while others are previously documented for plant architecture and chlorophyll content. Two SNPs positioned on chromosome 13 and 15 with previous QTLs reported for plant height and seed set, weight and abortion were associated with HI. WGR showed high predictive ability for Ei, RUE, and HI with maximum correlation ranging between 0.75 to 0.80. Both directed and undirected multivariate explanatory models indicate that HI has a strong relationship with A, average growth rate of canopy coverage for the first 40 days after planting (AGR40), seed-filling (SFL), and reproductive length (RL). According to the path analysis, increase in one standard unit of HI promotes changes in 0.5 standard units of GY, while changes in the same standard unit of RUE, and Ei produce increases on GY of 0.20 and 0.19 standard units. This study presents novel genetic knowledge for Ei, RUE, HI and GY along with a set of tools that may contribute to the development of new cultivars with enhanced light interception, light conversion and optimized dry matter partitioning in soybean. This work not only complements the physiological knowledge already available with the genetic control of traits directly associated with yield, but also represents a pioneer attempt to integrate traditional physiological traits into the breeding process in the context of physiological breeding<br></p>