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The purpose of this study is to design a real-time control and simulation system for a chemical mix facility. A simulation circuit board and software simulation in an IBM personal computer emulated the real-time chemical mix facility. A second personal computer controlled the plant. The parallel port in the IBM PC computer serves as a communication path between the controlled and controlling system. Results show that the simulation can assist the design of the actual system.

published_or_final_version / abstract / toc / Computer Science and Information Systems / Master / Master of Philosophy

published_or_final_version / Computer Science / Master / Master of Philosophy

Molecular diagnostics offer quick access to information for healthcare decision-making towards personalized therapeutics, but complicated procedures requiring extensive labor and infrastructure restrict their use. Droplet-based technologies can expand the accessibility of molecular diagnostics by miniaturizing devices, shortening sample-to-answer times, decreasing costs and increasing throughput. Methods for droplet manipulation are central to the automation of molecular diagnostics protocols. The innovative method, wire-guided droplet manipulation (WDM), is the actuation of liquid droplets in a hydrophobic milieu with a wire, or needle, guide. In this work, WDM is demonstrated for the automation of the polymerase chain reaction (PCR) on reprogrammable platforms for the diagnosis of cardiovascular infections. WDM is used to minimize thermal resistance by convective heat transfer for PCR amplification at a maximum speed of 8.67 s/cycle. The oil-water interfacial boundary is shown to passively partition molecular contaminants from sample matrices, including blood and heart valve tissue. Molecular self-assembly at the oil-water interface is used to increase PCR efficiency with blood in situ and is used as an innovative sensing modality for real-time monitoring of PCR amplification. Temperature feedback controlled droplet actuation is achieved by using a thermocouple loop as a functionalized wire-guide. Our novel methodology for real-time PCR, droplet-on-thermocouple silhouette real-time PCR (DOTS qPCR), utilizes interfacial effects to achieve droplet actuation, relief from PCR inhibitors and amplification sensing, for a sample-to-answer time as short as 3 min 30 s. DOTS qPCR addresses three major issues for rapid PCR—sample preparation, rapid thermocycling and sensitive real-time detection—on an inexpensive, disposable device with smartphone-based detection. In contrast, commercially available real-time PCR systems rely on fluorescence detection, have substantially higher threshold cycles, and require expensive optical components and extensive sample preparation. Due to the advantages of low threshold cycle detection we anticipate extending this technology towards trending biological research applications such as single cell, single nucleus, and single DNA molecule analyses, especially in droplet microfluidic platforms.

Rapid diagnosis of infectious disease and timely initiation of proper clinical antibiotic treatment is the determinant in obtaining the optimal clinical outcomes and reducing emergences of multidrug-resistant organisms. In particular, acute infections require the detection to be accomplished in limited time with high sensitivity due to the low concentration of organisms causing the infections. Real-time Polymerase Chain Reaction can provide quantitative identification of specific genetic materials and has revolutionized clinical microbiology laboratory diagnosis. It is becoming a standard for infectious disease detection. However, most real-time PCR instruments on the market are bulky, fragile and costly due to their delicate optical components, which restricted their use to point-of-care application. Modern microfluidic and sensing technology provide a transition from benchtop real-time PCR to miniaturizable, robust, and portable real-time PCR devices to achieve rapid, low-cost, and efficient point-of-care diagnosis. In this work, an innovative electrokinetic PCR (EK-PCR) platform that combines AC electrothermal flow (ACEF) and Joule heating induced temperature gradient to implement thermal cycling for DNA amplification is discussed. In addition, in situ electrochemical sensing is incorporated in the EK-PCR chamber for real-time monitoring of the DNA concentration toward quantification of the initial copies of the DNA template. EK-PCR can improve the energy efficiency with minimized total thermal mass and remain high amplification efficiency. More importantly, it represents a highly integrated strategy for portable point-of-care devices.

No description available.

No description available.

Το πρότυπο IEC61499 ορίζει το Function Block ως νέο τρόπο ανάπτυξης συστημάτων ελέγχου και αυτοματισμού. Τα συστήματα αυτά αποτελούνται από κατανεμημένες, ενσωματωμένες συσκευές οι οποίες διασυνδέονται μέσω βιομηχανικών δικτύων πραγματικού χρόνου. Λόγω του κατανεμημένου χαρακτήρα των συστημάτων αυτών, η εύρεση και επιδιόρθωση σφαλμάτων και ο έλεγχος της ορθής λειτουργίας τους θα πρέπει να γίνεται στο περιβάλλον των ενσωματωμένων αυτών συστημάτων. Στα πλαίσια αυτής της διπλωματικής δίνεται μία υλοποίηση για την κάλυψη της παραπάνω ανάγκης. Ο χρήστης μέσω μίας γραφικής διεπαφής, έχει την δυνατότητα να εκτελεί βασικές λειτουργίες απασφαλμάτωσης στο περιβάλλον των ενσωματωμένων συστημάτων, σε πραγματικό χρόνο, με την χρήση του RTAI και του RTnet. / The IEC 61499 standard defines Function Block as a new way of developing control and automation systems. These systems consist of distributed embedded devices which interconnect via real time industrial networks. Due to the distributive character of these systems, debugging and operation integrity check, should be done on target environment. This dissertation provides a tool for covering this need. Via a graphical user interface the user has the ability to perform basic real time debugging operations in the target enviroment, using RTAI and RTnet.

No description available.