An automated on-line SPE-LC-MS/MS method was developed for the quantitation of multiple classes of antibiotics in environmental waters. High sensitivity in the low ng/L range was accomplished by using large volume injections with 10-mL of sample. Positive confirmation of analytes was achieved using two selected reaction monitoring (SRM) transitions per antibiotic and quantitation was performed using an internal standard approach. Samples were extracted using online solid phase extraction, then using column switching technique; extracted samples were immediately passed through liquid chromatography and analyzed by tandem mass spectrometry. The total run time per each sample was 20 min. The statistically calculated method detection limits for various environmental samples were between 1.2 and 63 ng/L. Furthermore, the method was validated in terms of precision, accuracy and linearity. The developed analytical methodology was used to measure the occurrence of antibiotics in reclaimed waters (n=56), surface waters (n=53), ground waters (n=8) and drinking waters (n=54) collected from different parts of South Florida. In reclaimed waters, the most frequently detected antibiotics were nalidixic acid, erythromycin, clarithromycin, azithromycin trimethoprim, sulfamethoxazole and ofloxacin (19.3-604.9 ng/L). Detection of antibiotics in reclaimed waters indicates that they can’t be completely removed by conventional wastewater treatment process. Furthermore, the average mass loads of antibiotics released into the local environment through reclaimed water were estimated as 0.248 Kg/day. Among the surface waters samples, Miami River (reaching up to 580 ng/L) and Black Creek canal (up to 124 ng/L) showed highest concentrations of antibiotics. No traces of antibiotics were found in ground waters. On the other hand, erythromycin (monitored as anhydro erythromycin) was detected in 82% of the drinking water samples (n.d-66 ng/L). The developed approach is suitable for both research and monitoring applications. Major metabolites of antibiotics in reclaimed wates were identified and quantified using high resolution benchtop Q-Exactive orbitrap mass spectrometer. A phase I metabolite of erythromycin was tentatively identified in full scan based on accurate mass measurement. Using extracted ion chromatogram (XIC), high resolution data-dependent MS/MS spectra and metabolic profiling software the metabolite was identified as desmethyl anhydro erythromycin with molecular formula C36H63NO12 and m/z 702.4423. The molar concentration of the metabolite to erythromycin was in the order of 13 %. To my knowledge, this is the first known report on this metabolite in reclaimed water. Another compound acetyl-sulfamethoxazole, a phase II metabolite of sulfamethoxazole was also identified in reclaimed water and mole fraction of the metabolite represent 36 %, of the cumulative sulfamethoxazole concentration. The results were illustrating the importance to include metabolites also in the routine analysis to obtain a mass balance for better understanding of the occurrence, fate and distribution of antibiotics in the environment. Finally, all the antibiotics detected in reclaimed and surface waters were investigated to assess the potential risk to the aquatic organisms. The surface water antibiotic concentrations that represented the real time exposure conditions revealed that the macrolide antibiotics, erythromycin, clarithromycin and tylosin along with quinolone antibiotic, ciprofloxacin were suspected to induce high toxicity to aquatic biota. Preliminary results showing that, among the antibiotic groups tested, macrolides posed the highest ecological threat, and therefore, they may need to be further evaluated with, long-term exposure studies considering bioaccumulation factors and more number of species selected. Overall, the occurrence of antibiotics in aquatic environment is posing an ecological health concern.

Increased demands on water supplies caused by population expansion, saltwater intrusion, and drought have led to water shortages which may be addressed by use of recycled water as recycled water products. Study I investigated recycled water production in Florida and California during 2009 to detect gaps in distribution and identify areas for expansion. Gaps were detected along the panhandle and Miami, Florida, as well as the northern and southwestern regions in California. Study II examined gaps in distribution, identified temporal change, and located areas for expansion for Florida in 2009 and 2015. Production increased in the northern and southern regions of Florida but decreased in Southwest Florida. Recycled water is an essential component water management a broader adoption of recycled water will increase water conservation in water-stressed coastal communities by allocating recycled water for purposes that once used potable freshwater.

From the Proceedings of the 1974 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 19-20, 1974, Flagstaff, Arizona / A plan for the reuse of sewage effluent is proposed for the city of Tucson, Arizona. Several kinds of use would be possible, but utilization for irrigation of existing farmland in the Avra-Marana area seems particularly attractive for several reasons: (1) conveyance can be accomplished by gravity flow, (2) no tertiary treatment is required for the presently grown crops, (3) the nutrients in the effluent would be better used, and (4) effluent use would reduce the pumpage of high quality groundwater, conserving it for municipal or other uses. An exchange of wastewater for groundwater for use in the city system is seen as a good alternative to the present practice of the city purchasing farmland in Avra valley in order to acquire the groundwater for conveyance to the Tucson basin. Objectives to maximize the quantity and efficiency of wastewater use may not appear compatible with the profit maximization motive of the individual farmer, and suitable provisions will have to be written into wastewater sales agreements to assure coordination between user and supplier.

This report analyzes the current greenhouse gas emissions inventory for The University of Texas at Austin (UT-Austin), reviews the carbon reduction strategies being implemented at UT-Austin and other peer institutions, and offers recommendations for strategies that could reduce greenhouse gas emissions at UT-Austin in the future. / text

From the Proceedings of the 1975 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 11-12, 1975, Tempe, Arizona / A direct osmosis plant can reclaim twenty to thirty thousand acre feet of Wellton-Mohawk brackish drainage water using no more nitrogen fertilizer than is normally used in the Yuma, Coachella valley, Imperial Valley and the bordering Mexican areas. On a per-acre basis ammonium sulfate-driven direct osmosis can reclaim about one percent of the total irrigation requirement from 3000 ppm brackish water. In addition to the ammonium sulfate-driven direct osmosis efficiency, the by-product energy recovery of the manufacture of the fertilizer and the low technology inherent in direct osmosis processes make direct osmosis an appealing water reclaiming process.

From the Proceedings of the 1978 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona Academy of Science - April 14-15, 1978, Flagstaff, Arizona / With the objective of determining if sufficient water would be salvaged by a typical desert, urban-household from normally wasted sources associated with the lot and household to adequately irrigate a garden and orchard, a 2000 sq ft house on a typical one fifth acre lot in three cities having climates similar to Phoenix, Tucson, or Prescott, Arizona was hypothesized and the amount of water available for yard watering calculated, provided that (1) only rainfall was available, (2) rainfall-runoff from covered areas associated with or adjacent to the lot was salvaged (roof, street, alley etc.), (3) gray-water from the household was utilized, (4) a portion of the lot was waterproofed to concentrate the runoff on the untreated portion, and (5) various combinations of the above were utilized to increase the amount of available water. It is demonstrated that these sources could be used singly or in combination to obtain the required amount of water with the actual amount available depending upon the precipitation, runoff and runon areas, runoff efficiency of the contributing area, and the number of people in the household. A number of horticultural plants are suggested that should best fit such an irregular irrigation scheme.