Spelling suggestions: "subject:"reperfusion"" "subject:"eperfusion""
151 |
Cardiac ischemia reperfusion injury and the role of neutral sphingomyelinase /O'Brien, Nicole Wadsworth. January 2003 (has links)
Thesis (Ph. D.)--University of California, San Diego, 2003. / Vita. Includes bibliographical references (leaves 162-178).
|
152 |
Preconditioning with LPS of porphyromonas gingivalis confers delayed cardiac functional protection against ischemia and reperfusionWong, Ka-li., 黃嘉莉. January 2007 (has links)
published_or_final_version / Medicine / Master / Master of Medical Sciences
|
153 |
Endotoxin from porphyromonas gingivalis improves recovery of the electrically induced Ca2+ transient following ischemia andreperfusionFan, Man-hin, Michael., 范文軒. January 2007 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
|
154 |
The effect of intravenous and intrathecal morphine preconditioning on hepatic ischaemia-reperfusion injury in normal and cirrhotic liversWang, Yuan, 王苑 January 2012 (has links)
Hepatic ischaemia-reperfusion injury occurs when patients undergoing liver
operations such as liver transplantation, tumour resection and shock. Intravenous and
intrathecal administration of morphine can be used to provide analgesia prior or after
liver surgery. It has been reported that systemically administered morphine conferred
protective effect on numerous organs, including heart, brain and kidney. The focus of
my research is to investigate the effect of intravenous and intrathecal morphine
preconditioning on normal and cirrhotic livers. Further, PI3K/Akt, STAT3 and
HO-1/iNOS pathways had been shown to ameliorate hepatic ischemia-reperfusion
injury. Hence, we aim to investigate these possible signaling pathways associated with
morphine mediated hepato-protection.
A partial hepatic ischaemia reperfusion injury model in rats was used. The
experiments were divided into two series: one involved in normal livers and the other
one involved in cirrhotic livers. For the normal livers, morphine at different doses
were administrated intravenously or intrathecally prior the onset of ischaemia, and the
experiments were repeated with previous intravenous administration of naloxone
methiodide (opioid receptor antagonist), or wortmannin (Akt inhibitor), respectively.
For the cirrhotic livers, morphine at optimal doses were injected intravenously or
intrathecally prior the onset of ischaemia. Those rats with only induced hepatic
ischaemia-reperfusion injury only were marked as control groups. The effect of
morphine preconditioning on hepatic architecture, apoptosis and liver function were
evaluated respectively by hematoxylin-eosin (H&E) staining, Terminal
deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) staining, the
expression of cleaved Caspase-3, and serum levels of alanine aminotransferase (ALT)
and aspartate aminotransferase (AST). Meanwhile, the expression of phosphorylated
Akt, phosphorylated JAK2, phosphorylated STAT3, HO-1 and iNOS were detected by
Western Blot to determine the signaling pathways involved by intravenous and
intrathecal morphine preconditioning.
The normal livers series presented intravenous and intrathecal morphine
preconditioning at the 100μg/kg, 10μg/kg, respectively, better persevered hepatic
architecture when compared with control groups. The degree of liver cell apoptosis
and expression of cleaved caspase-3 were also reduced by intravenous and intrathecal
morphine preconditioning. In additional, intravenous and intrathecal morphine
preconditioning ameliorated hepatocellular damage by reducing ALT&AST release.
Moreover, the expressions of phosphorylated Akt and its downstream protein STAT3
were significantly increased by intravenous and intrathecal morphine preconditioning,
compared with their respective control groups. The hepato-protective effect of
intravenous and intrathecal morphine preconditioning was reversed by naloxone
methiodide or wortmannin pretreatment. The similar pattern of protection was
observed in cirrhotic livers. Both intravenous and intrathecal morphine
preconditioning protected hepatic architecture much better than control groups. They
also attenuated hepatic apoptosis degree and hepatocellular enzyme release.
Furthermore, the expression of HO-1 was up-regulated, whereas the expression of
iNOS was down-regulated by intravenous and intrathecal morphine preconditioning.
In summary, this study provided evidence that intravenous and intrathecal
morphine preconditioning could attenuate hepatic ischaemia-reperfusion injury in
normal and cirrhotic livers. The involvement of opioid receptors, Akt/STAT3 pathway
and HO-1 pathway might be the underlying mechanisms of morphine
hepato-protection. Finally, the protective effect of morphine preconditioning might
provide a potential therapeutic approach for clinical usage. / published_or_final_version / Anaesthesiology / Master / Master of Philosophy
|
155 |
A systems pharmacology approach to discovery of drugs to ameliorate oxidant stress in human endothelial cellsBynum, James Andrew, Jr. 08 September 2015 (has links)
Ischemia is characterized by reduced blood flow to an area of the body which can then cause cellular injury through the generation of reactive oxygen species (ROS), activation of inflammation, and induction of apoptosis. Although rapid reestablishment of flow is required to prevent organ death, the reperfusion phase of this injury can cause its own deleterious effects often exacerbating the initial insult. The combined action of the two injuries is termed ischemia/reperfusion (I/R) injury. Oxidative stress that results from ischemia/reperfusion injury is a common pathological condition that accompanies many human diseases including stroke, heart attack and traumatic injury. In addition, neurodegenerative diseases including Parkinson’s, Alzheimer’s, and Huntington’s disease appear to involve oxidative stress.
Although actively investigated by the medical and pharmaceutical industry; limited progress has been made to ameliorate I/R injury and to date there is no drug approved for treatment for I/R injury. Therapeutic approaches to treat I/R injury have included the administration of compounds to scavenge ROS or induce protective pathways or genetic responses. It was previously reported that caffeic acid phenethyl ester (CAPE), a plant-derived polyphenol, displayed cytoprotective effects against menadione (MD)-induced oxidative stress in human umbilical vein endothelial cells (HUVEC), and the induction of heme oxygenase-1 (HMOX1), a phase II enzyme, played an important role for CAPE cytoprotection.
In an effort to improve this cytoprotection, other phase II enzyme inducers were investigated and, 2-cyano-3,12 dioxooleana-1,9 dien-28-imidazolide (CDDO-Im) and 2-cyano-3,12-dioxooleana-1,9-dien-28-oyl methyl ester (CDDO-Me), were found to be potent inducers with a rapid onset of action. CDDO-Im and CDDO-Me, synthetic olenane triterpenoids, developed as anticancer agents were compared to CAPE revealing that CDDO-Im was a more potent inducer of Phase II enzymes including HMOX1 and provided better cytoprotection than CAPE. Gene expression profiling showed that CDDO-Im was more potent inducer of protective genes like HMOX1 than CAPE and additionally induced heat shock proteins. To better understand the mechanism of action of CDDO-IM, a gene expression time-course was undertaken to identify early initiators of the transcriptional response preceding cytoprotection. Application of systems pharmacology identified molecular networks of cell mediating processes.
|
156 |
The in vivo mechanism of actions of mycophenolate mofetil: insights from murine models of allograft rejection,endotoxemia, ischemia reperfusion injury and lupus nephritisLui, Sing-leung., 雷聲亮. January 2003 (has links)
published_or_final_version / abstract / toc / Medicine / Master / Doctor of Medicine
|
157 |
The effect of herbal medicine on renal ischemia/reperfusion injuryLok, Lap-kwan, Marco., 陸立羣. January 2002 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
|
158 |
The role of Ca2+ in protection of preconditioning and ischaemia-induced injury in the rat heartYan, Wing-yi., 殷詠儀. January 2003 (has links)
published_or_final_version / abstract / toc / Physiology / Master / Master of Philosophy
|
159 |
Analysis of nitric oxide generation in various organs of animal modelsduring ischemia-reperfusion張曉暉, Zhang, Xiaohui. January 1999 (has links)
published_or_final_version / Physics / Master / Master of Philosophy
|
160 |
Role of Chinese medicinal compounds in the regulation of stress-activated protein kinase in ischaemic/reperfused rat heart歐楊嘉慧, Au-Yeung, Ka-wai. January 2000 (has links)
published_or_final_version / Pharmacology / Master / Master of Philosophy
|
Page generated in 0.0766 seconds