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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Controversial Issues Related To Reproductive Biotechnology: An Empirical Study

Ocak, Gulsevim Evsel 01 February 2012 (has links) (PDF)
This study examines the problems which are created by assisted reproductive techniques on the individuals and their decisions about the reproduction. In the study, the data of a field study which was conducted in 2010 is used in order to make the examination deeper and to give a qualitative and quantitative dimension to the theoretical framework. Through the sociological analysis of both controversial issues occurred by pre-natal reproductive technologies such as sex selection, abortion, PGD, IVF babies, disability, etc. and personal decisions which are impossible to be given independent from the social environment, providing a contribution to the development of sociology of reproduction is desired. In this study it is claimed that assisted reproductive techniques are power which will possibly get ahead of natural reproduction and reduce and even erase the biodiversity and coincidental characteristics of human reproduction, and increase the inequalities in the society. Thus this power may courage the reproduction of &lsquo / desirables&rsquo / and prevent &lsquo / undesirable&rsquo / ones from living and even insemination anymore. Another argumentation which is under discussion is the illusion of all these activities and problems were taking their sources from the own decisions of prospective parents. Reproductive biotechnology commerce hopes to people through its economy, cuts across all boundaries through the bounties of its technical abilities and by doing so it does not see a drawback in making people &lsquo / victims&rsquo / of their choices that regarded as &lsquo / rational and free&rsquo / which in fact mere &lsquo / irrational&rsquo / preferences. Thus in this thesis, the theoretical foundations and social results of this technology which extending up to the pre-natal processes are discussed to contribute a more democratic policies
2

Avaliação de protocolos de superovulação em cervos-do-pantanal (Blastocerus dichotomus) / Evaluation of superovulation protocols in marsh deer (Blastocerus dichotomus)

Galindo Huamán, David Javier 24 February 2017 (has links)
Submitted by DAVID JAVIER GALINDO HUAMÁN null (dgalindoh89@gmail.com) on 2017-04-06T12:09:49Z No. of bitstreams: 1 galindo_dj_me_jabo.pdf: 3220960 bytes, checksum: b0645c4310022e5f5c72093f60c926a4 (MD5) / Approved for entry into archive by Luiz Galeffi (luizgaleffi@gmail.com) on 2017-04-12T18:50:35Z (GMT) No. of bitstreams: 1 galindohuaman_dj_me_jabo.pdf: 3220960 bytes, checksum: b0645c4310022e5f5c72093f60c926a4 (MD5) / Made available in DSpace on 2017-04-12T18:50:35Z (GMT). No. of bitstreams: 1 galindohuaman_dj_me_jabo.pdf: 3220960 bytes, checksum: b0645c4310022e5f5c72093f60c926a4 (MD5) Previous issue date: 2017-02-24 / Outra / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O cervo-do-pantanal (Blastocerus dichotomus) é uma espécie emblemática dentro dos cervídeos neotropicais. Nas últimas décadas, suas populações têm sofrido declínio notório devido, principalmente, à destruição do seu habitat. Isto causa a perda da diversidade genética, que junto à depressão endogâmica (pelo isolamento de pequenas populações) pode levar à espécie a extinções locais. Nesse contexto, as técnicas de reprodução assistida podem auxiliar no processo de manutenção da diversidade genética nestas populações isoladas. Desta forma, o objetivo deste estudo foi elaborar um protocolo de superovulação para cervo-do-pantanal em cativeiro. Assim, foram testados protocolos sucessivamente (A, B, C) até que fossem obtidos resultados satisfatórios. Para que se pudesse chegar a um protocolo viável, foram testadas três combinações farmacológicas. Tratamento A: CIDR® durante sete dias, seguido de 0,5 mg de BE e 0,11 mg de GnRH no dia 0, 800UI de eCG no dia 5 após inserção do CIDR®, 530 µg de PGF2α no dia 7 e 0,11 mg de GnRH logo após a detecção do estro, para a indução da ovulação. Tratamento B: CIDR® durante oito dias, seguido de 0,5 mg de BE e 0,11 mg de GnRH no dia 0, 1200UI de eCG no dia 5 após inserção do CIDR®, 530 µg de PGF2α no dia 8 e 0,11 mg de GnRH logo após a detecção do estro, para a indução da ovulação. Tratamento C: CIDR® durante oito dias, seguido de 0,5 mg de BE e 0,11 mg de GnRH no dia 0, 1200UI de eCG no dia 5 após inserção do CIDR®, 530 µg de PGF2α no dia 8 e 2,5 mg de LH, entre 12-18 horas após a detecção do estro para a indução da ovulação. A detecção de estro foi realizada com o auxílio de um macho, sendo permitida a cópula. Oito dias após a cópula foi realizada a contagem dos CL e folículos anovulatórios mediante laparotomia mediana ventral, assim como a colheita de embriões. O Tratamento A resultou em 2 CL. O Tratamento B resultou em 1 CL e 8 folículos anovulatórios. O Tratamento C resultou em 10, 3 e 11 CL, e 0, 1 e 5 folículos anovulatórios para as fêmeas 3, 4 e 5, respectivamente. Além disso foi realizada a colheita de 2, 2 e 5 embriões das fêmeas FBD3, FBD4 e FBD5, respectivamente. Obtendo uma taxa de recuperação de 37,5% (9/24), dos quais dois eram embriões viáveis para transferência. Todos os tratamentos foram efetivos na sincronização do estro. O Tratamento C, com a aplicação i.m. de 1200UI de eCG e de LH, como indutor da ovulação, teve a melhor resposta em termos de superovulação, assim como de colheita de embriões. / The marsh deer (Blastocerus dichotomus) is an emblematic species within the neotropical deer. In the last decades, their populations have suffered a notable decline due, mainly, to the destruction of their habitat. This causes loss of genetic diversity, which together with inbreeding depression (by the isolation of many small populations) can lead this species to local extinctions. In this context, assisted reproduction techniques can help in the process of maintaining genetic diversity in these isolated populations. Thus, the aim of this study was to elaborate a superovulation protocol for captive marsh deer. Therefore, protocols were successively tested (A, B, C) until satisfactory results were obtained. To be able to achieve a viable protocol, three pharmacological combination were tested. Treatment A: CIDR® for 7 days, followed by 0.5 mg of EB and 0.11 mg of GnRH on D0, 800IU of eCG on day 5 after CIDR® insertion, 530 µg PGF2α on day 7, and 0.11 mg of GnRH soon after estrus detection, for ovulation induction. Treatment B: CIDR® for 8 days, followed by 0.5 mg of EB and 0.11 mg of GnRH on D0, 1200IU of eCG on day 5 after CIDR® insertion, 530 µg PGF2α on day 8, and 0.11 mg of GnRH soon after estrus detection, for ovulation induction. Treatment C: CIDR® for 8 days, followed by 0.5 mg of EB and 0.11 mg of GnRH on D0, 1200IU of eCG on day 5 after CIDR® insertion, 530 µg PGF2α on day 8, and 0.25 mg of LH, between 12-18 hours post estrus detection, for ovulation induction. Estrus detection was performed with the aid of a male, and copulation was allowed. Eight days after copulation, CL and anovulatory follicles were counted through median ventral laparotomy, as well as the collection of embryos. Treatment A resulted in 2 CL. Treatment B resulted in 1 CL and 8 anovulatory follicles. Treatment C resulted in 10, 3 and 11 CL, and 0, 1 and 5 anovulatory follicles for hinds FBD3, FBD4, and FBD5, respectively. In addition, 2, 2 and 5 embryos were harvested from hinds FBD3, FBD4 and FBD5, respectively. Obtaining a recovery rate of 37.5% (9/24), of which two were viable embryos for transfer. All treatments were effective in synchronizing estrus. Treatment C, with an i.m. application of 1200IU of eCG and LH, as ovulation inducer, had the best response in terms of superovulation, as well as embryo collection.

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