Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:34:44Z No. of bitstreams: 5 ric.pdf: 5207435 bytes, checksum: f03a11b8bcdc89eef456751ccb1f74f2 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T10:28:40Z (GMT) No. of bitstreams: 5 ric.pdf: 5207435 bytes, checksum: f03a11b8bcdc89eef456751ccb1f74f2 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-20T10:28:40Z (GMT). No. of bitstreams: 5 ric.pdf: 5207435 bytes, checksum: f03a11b8bcdc89eef456751ccb1f74f2 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-09-20 / A pesquisa b?sica e aplicada voltada para o conhecimento e produ??o de celulases e xilanases microbianas constituem-se em valorosa a??o para o desenvolvimento de produtos e processos biotecnol?gicos. Estas enzimas t?m sido utilizadas na ind?stria de alimentos, cervejarias, vin?colas, produ??o de ra??o, ind?stria t?xtil e fabrica??o de papel. A demanda por tais catalisadores tem crescido rapidamente, encorajada principalmente pela ind?stria de biocombust?veis, em especial para a produ??o de etanol de 2? Gera??o. Neste contexto, o objetivo deste trabalho foi produzir enzimas celulol?ticas e xilanol?ticas com o uso de fungos filamentosos de ocorr?ncia natural, utilizando res?duos s?lidos da cadeia produtiva do biodiesel como fontes de carbono. Tr?s linhagens de fungos filamentosos isoladas de amostras ambientais foram avaliadas para produ??o de celulases e xilanases. Como par?metro de compara??o, utilizou-se o Trichoderma reesei CCT2768, linhagem conhecida como produtora de celulases. Dentre as linhagens avaliadas, um isolado denominado Aspergillus sp AN1257 foi o que se destacou para a produ??o de enzimas celulol?ticas e xilanol?ticas, superando inclusive a produ??o de celulases por T. reesei nas mesmas condi??es fermentativas. A fonte de carbono que melhor induziu a produ??o das enzimas investigadas pelo Aspergillus sp AN1257 foi a torta de caro?o de algod?o. Em busca da condi??o ?tima para a produ??o das enzimas estudadas foi realizado um planejamento fatorial e posteriormente aplicado um delineamento composto central rotacional (DCCR). Ap?s a aplica??o das condi??es ?timas para a produ??o enzim?tica desejada, o extrato obtido apresentou valores de 3,12 U/mL para atividade endoglucan?sica e 76,4 U/mL para a atividade xilan?sica. O extrato enzim?tico produzido apresentou atividade promissora quando utilizado na hidr?lise de lignocelulose em alguns res?duos agroindustriais. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2012. / ABSTRACT The basic and applied research focused on the knowledge and production of microbial cellulases and xylanases constitute a valuable action for the development of biotechnology products and processes. These enzymes have been used in the food industry, breweries, wineries, feed production, textile and paper manufacturing. The demand for such catalysts has grown rapidly, encouraged mainly by the biofuels industry, in particular for the production of 2nd Generation ethanol. In this context, the aim of this work was to produce cellulolytic and xylanolytic enzymes using naturally occurring filamentous fungi and solid waste of the biodiesel productive chain as carbon source. Three strains of filamentous fungi isolated from ambient samples have been evaluated for production of cellulases and xylanases. As reference microbial strain, it was used Trichoderma reesei CCT2768, known a good producer of cellulases. Among the strains tested, an isolated named Aspergillus sp AN1257 stood out for the production of cellulolytic and xylanolytic activity, surpassing even the production of cellulases by T. reesei under the same fermentation conditions. The carbon source which better induced the production of the investigated enzymes by Aspergillus sp AN1257 was cottonseed cake. In search of the optimum condition for the production of the investigated enzymes it was performed a factorial design and then it was applied a rotational central composite design (RCCD). After application of optimal condiction for enzyme production, the extract obtained showed values ??of 3.12 U/mL for endoglucanasic activity and 76.4 U/mL for xylanolytic activity. The enzyme extract produced showed promising application when used in the hydrolysis of lignocellulose in some agro-industrial residues.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-04-10T15:37:36Z No. of bitstreams: 1 2010 - Gabriela Fernandes Samico.pdf: 726920 bytes, checksum: 6b47b0e0b5e09678a7fcd0004c798bd6 (MD5) / Made available in DSpace on 2018-04-10T15:37:36Z (GMT). No. of bitstreams: 1 2010 - Gabriela Fernandes Samico.pdf: 726920 bytes, checksum: 6b47b0e0b5e09678a7fcd0004c798bd6 (MD5) Previous issue date: 2010-05-13 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / The passionfruit (Passiflora edulis, F. flavicarpa, Deg) it is fruit originary of America Tropical, that deserves prominence for the sensorial characteristics of the juice that produces, traditionally consumed in natural way or industrialized, being important source of minerals and vitamins, as calcium, phosphorous and vitamin C. Brazil is the main producing and consuming country of passionfruit. The excellent acceptance of this juice has been responsible for the increment of culture of passionfruit, the industries extracting machines only use to advantage the pulp, that represents about 30% of the weight of the fruit, the remain, peel and seeds, that according to some authors, 70% of the all up weight correspond, when used in the feeding of animals are not thrown in the garbage, without no treatment, causing serious problems to the ecosystem, with consequences in the health of the bordering population. This work had objective to develop technology for oil obtention of seeds of passionfruit feeding human being and to characterize its physical and chemical properties, and to evaluate proteinic and energy the mineral content, of the defatted deeds, effluent of this process and uses it in the cookie elaboration. The analyses had disclosed that the seed of passionfruit partially dehydrated contained 1.7% of oil, consisting of 14,64% of oleic acid and 69.73% of linoleic acid. Tests of oxidation of this fraction showed that the OSI of this oil was of 11h. The defatted seeds have 8.44% of humidity on average, 0.71% of reducing carbohydrates, 1.82% of not reducing carbohydrates, 4.22% of starch, 31.42% of protein, 1.18% of lipids, 3.64% of soluble fibers, 64.30% of insoluble fibers and 1.38% of leached ashes. The sensorial tests show the preference for the formularization that contained 8% of Flour of seed taken away the fat of passionfruit. The perspective of exploitation of this residue of the manufacter process, will become the agro-industrial one of passionfruit more income-producing, since they also exist alternative viable of exploitation of the rind, with this, is possible to implant in the agroindustry of passionfruit, so dreamed clean technology. / O maracuj? (Passiflora edulis, f. flavicarpa, Deg) ? um fruto origin?rio da Am?rica Tropical, que merece destaque pelas caracter?sticas sensoriais do suco que produz,, tradicionalmente consumido ao natural ou industrializado, sendo importante fonte de minerais e vitaminas, como c?lcio, f?sforo e vitamina C. O Brasil ? o principal pa?s produtor e consumidor de maracuj?-amarelo. A excelente aceita??o desse suco tem sido respons?vel pelo incremento de cultivo de maracuj?, que para sua obten??o, as ind?strias extratoras aproveitam somente a polpa, que representa cerca de 30% do peso do fruto, o restante, casca e sementes, que segundo alguns autores, correspondem a 70% do peso total, quando n?o utilizadas na alimenta??o de animais s?o jogadas no lixo, sem nenhum tratamento, causando s?rios problemas ao ecossistema, com reflexos na sa?de das popula??es lim?trofes aos descartes. Este trabalho teve como objetivo desenvolver tecnologia para obten??o de ?leo de sementes de maracuj? para alimenta??o humana e caracterizar suas propriedades f?sicas e qu?micas, e avaliar o conte?do mineral, prot?ico e energ?tico da torta desengordurada, efluente desse processo e utiliza-lo na elabora??o de biscoito tipo ?cookie?. As an?lises revelaram que a semente de maracuj? parcialmente desidratada continha 26% de ?leo, constitu?do de 14,64% de ?cido ol?ico e 69,73% de ?cido linol?ico. Testes de oxida??o desta fra??o mostraram que o ?ndice de estabilidade oxidativa deste ?leo foi de 11horas. A torta possui em m?dia 8,44 % de umidade, 0,71% de glic?dios redutores, 1,82% de glic?dios n?o redutores, 4,22% de amido, 31,42% de prote?na, 1,18% de lip?deos, 3,64% de fibras sol?veis, 64,30% de fibras insol?veis e 1,38% de cinzas. Os testes sensoriais apresentaram a prefer?ncia pela formula??o que continha 8% de Farinha de semente desengordurada de maracuj?. A perspectiva de aproveitamento desse res?duo do processo fabril, tornar? a agroindustrial do maracuj? mais rent?vel, j? que existem tamb?m alternativas vi?veis de aproveitamento das casca, com isso, ? poss?vel implantar no agroneg?cio do maracuj?, a t?o sonhada tecnologia limpa.