Les mécanismes de formation de phases dans des films minces du système ternaire Al-Cu-Fe et des systèmes binaires Al-Cu, Al-Fe et Cu-Fe ont été étudiés. Dans chacun des systèmes, plusieurs échantillons avec des compositions distinctes ont été préparés par pulvérisation cathodique. Des couches d’aluminium, de cuivre et de fer ont été déposées séquentiellement sur des substrats de silicium oxydé et ont été traités thermiquement par différentes méthodes puis caractérisés. Des mesures de diffraction de rayons X et de résistivité in-situ ont été effectuées pour suivre la formation des phases. Des recuits thermiques suivis de trempe ont été réalisés et les échantillons ont été caractérisés par diffraction des rayons X. L’analyse enthalpique différentielle a également été utilisée ainsi que des mesures simultanées in-situ de résistivité et de diffraction des rayons X. L’ensemble des résultats obtenus nous a permis de proposer des mécanismes de formation de phases pour chacun des échantillons étudiés et en utilisant des modèles théoriques de croissance de phases nous avons pu déterminer des données cinétiques sur la formation de phases dans ces films. / The mechanisms of phase formation in thin films have been studied in the Al-Cu, Al-Fe, Fe-Cu and Al-Cu-Fe systems. Several samples with different compositions have been prepared by sputtering. Aluminium, copper and iron layers were deposited onto oxidized silicon substrates, they were heat treated and characterized by using several techniques. In situ X-ray diffraction and resistivity measurements were used to follow the phase formation. Thermal annealings followed by quenching have also been carried out to get additional information.Differential Scanning Calorimetry and coupled in-situ resistivity and X-ray diffractionmeasurements were performed. The whole results allowed us to suggest a mechanism of phase formation for each sample and by using theoretical models of growth we determined kinetic data on the phase formation.

Tumor malignancies are the second leading cause of death worldwide. One of the reasons for the failure of oncological treatment are the uniformly set clinical guidelines, which neglect the effect of high intertumoral heterogeneity. The in vitro chemosensitivity and resistance (CSRA) assays allow for the stratification of patients prior to therapy. Therefore, the CSRA are a long-considered method for personalization of components of chemotherapy regime. Nevertheless, none of them is being routinely used in clinical practice. Certain chemotherapeutics used for their cytotoxic and cytostatic effect are also able to induce so-called immunogenic cell death (ICD) of tumor cells and activate an anti-tumor immune response. Monitoring of changes in the expression of molecules associated with the regulation of the innate immune system on the surface of dying tumor cells would enable to predict the patient's ability to respond to treatment involving modern immunotherapeutics. The feasibility of CSRA using flow cytometry and microscopy is critically evaluated in this thesis on a model of bladder cancer. Simultaneously, the correlation of the immunogenic phenotype of tumor cells and their sensitivity to selected chemotherapeutics is discussed.

The main focus of this thesis is to design the expansion and modernization of a sport complex "U Jezu" for a sport club TJ Roztoky. This thesis is consisted of a study for a territorial agreement. In this study, there are indicated a formation of two playing fields with the artificial surfaces, beach volleyball court proposing two building objects as a base for the entire complex, which consists of garages, storages and a main building. A more detailed design was created for the main building. As the supporting structure of the object, the transverse wall system completed with outdoor columns supporting the cantilevered portion of the ceiling tiles, which are mainly unidirectionally reinforced concrete floor slabs. The supporting structure is based on the strip foundations. The designed building object has two-above ground floors and a ground floor, which will be created by embedding the building object into the hill side. The ground floor is designed for the relaxation and the leisure purposes. Moreover, there is also a technological equipment necessary for the operation of the facility. On the first above ground floor, there are designed locker rooms for athletes, a club room with a kitchen and an accommodation unit for a manager of the complex. The second floor is for the accommodation. There are designed rooms with lounge and office of the president of the sport club. Part of the second floor consists of a terrace. For the roofing, a flat roof with a single-classic sequence of layers is designed. In this thesis, the static is partially solved. Furthermore, the object is evaluated in the terms of lighting, acoustic and thermal requirements as well as requirements for the fire safety of buildings.