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1	"Detecção de Staphylococcus aureus resistente à meticilina por meio de multiplex PCR em amostras de secreção respiratória de pacientes com fibrose cística" / Detection of methicillin-resistant Staphylococcus aureus by multiplex PCR in respiratory secretion of cystic fibrosis patients Monte, Luciana de Freitas Velloso 22 November 2005 (has links) S.aureus resistente à meticilina(SARM) é um problema em centros de fibrose cística(FC). Foi desenvolvido um multiplex PCR(mPCR) para detecção do SARM em secreção respiratória de 106 pacientes com FC. Foram usados 3 pares de primers para amplificar os genes: mecA, coa, 16S rRNA. O mPCR detectou até 0,25pg de DNA de SARM e identificou 70/106(66,0%) pacientes com S.aureus e 28/106(26,4%) com SARM. O mPCR mostrou especificidade, sensibilidade, valores preditivos positivo e negativo de 87,8%, 84,4%, 50% e 97,5%, considerando a cultura como padrão-ouro. Os resultados discordantes foram testados com outros primers, confirmando os obtidos pelo mPCR em 82/84. O mPCR mostrou-se método rápido e confiável para detecção de SARM / Methicillin-resistant S.aureus(MRSA) is a significant concern in cystic fibrosis(CF) centers. A multiplex PCR(MPCR) was developed to detect MRSA in respiratory secretion of 106 CF patients. Three pairs of primers were used for amplification of genes: mecA, coa, 16S rRNA. MPCR detected 0.25pg of MRSA DNA and identified 70/106(66.0%) of patients with S.aureus and 28/106(26.4%) with MRSA. MPCR showed specificity, sensitivity, positive and negative predicted values at 87.8%, 84.4%, 50% and 97.5%, considering culture as the gold standard. Discrepant results were retested using different primers, and confirmed MPCR results in 82/84. The developed MPCR was found to be a rapid and reliable method for MRSA detection Cystic fibrosis Fibrose cística Infecções respiratórias/diagnóstico Methicillin resistance Polymerase chain reaction Reação em cadeia por polimerase Resistência à meticilina Respiratory tract infections/diagnosis
2	"Detecção de Staphylococcus aureus resistente à meticilina por meio de multiplex PCR em amostras de secreção respiratória de pacientes com fibrose cística" / Detection of methicillin-resistant Staphylococcus aureus by multiplex PCR in respiratory secretion of cystic fibrosis patients Luciana de Freitas Velloso Monte 22 November 2005 (has links) S.aureus resistente à meticilina(SARM) é um problema em centros de fibrose cística(FC). Foi desenvolvido um multiplex PCR(mPCR) para detecção do SARM em secreção respiratória de 106 pacientes com FC. Foram usados 3 pares de primers para amplificar os genes: mecA, coa, 16S rRNA. O mPCR detectou até 0,25pg de DNA de SARM e identificou 70/106(66,0%) pacientes com S.aureus e 28/106(26,4%) com SARM. O mPCR mostrou especificidade, sensibilidade, valores preditivos positivo e negativo de 87,8%, 84,4%, 50% e 97,5%, considerando a cultura como padrão-ouro. Os resultados discordantes foram testados com outros primers, confirmando os obtidos pelo mPCR em 82/84. O mPCR mostrou-se método rápido e confiável para detecção de SARM / Methicillin-resistant S.aureus(MRSA) is a significant concern in cystic fibrosis(CF) centers. A multiplex PCR(MPCR) was developed to detect MRSA in respiratory secretion of 106 CF patients. Three pairs of primers were used for amplification of genes: mecA, coa, 16S rRNA. MPCR detected 0.25pg of MRSA DNA and identified 70/106(66.0%) of patients with S.aureus and 28/106(26.4%) with MRSA. MPCR showed specificity, sensitivity, positive and negative predicted values at 87.8%, 84.4%, 50% and 97.5%, considering culture as the gold standard. Discrepant results were retested using different primers, and confirmed MPCR results in 82/84. The developed MPCR was found to be a rapid and reliable method for MRSA detection Fibrose cística Infecções respiratórias/diagnóstico Reação em cadeia por polimerase Resistência à meticilina Cystic fibrosis Methicillin resistance Polymerase chain reaction Respiratory tract infections/diagnosis
3	Respiratory Infections in Ambulatory Adults. Choosing the Best Treatment Perlman, P E., Ginn, D R. 01 January 1990 (has links) The diagnosis and treatment of respiratory tract infections in ambulatory adults is challenging. The prevalence of these conditions outstrips the medical profession's efficiency and effectiveness in dealing with them. However, selecting diagnostic techniques that identify causative organisms and therapeutic agents targeted to those organisms should lead to a reduction in the morbidity and mortality associated with these illnesses. adult aged ambulatory care anti-bacterial agents (therapeutic use) bronchitis (diagnosis drug therapy etiology) child common cold (diagnosis etiology prevention & control therapy) diagnosis differential epiglottitis (diagnosis) humans influenza human (diagnosis etiology prevention & control therapy) middle aged otitis media (diagnosis drug therapy) pharyngitis (diagnosis drug therapy etiology) pneumonia (diagnosis therapy) respiratory tract infections (diagnosis etiology therapy) sinusitis (diagnosis drug therapy etiology) virus diseases (complications) QCOM

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