With life expectancy on the rise, age-related ailments are a significant strain on the welfare of individuals and the economy. Progress is being made towards combating the leading cause of unavoidable blindness, age-related macular degeneration (AMD). AMD affects ten million Americans and costs the world economy $343 billion annually. Retinal implants promise to restore sight by replacing the eye's damaged photoreceptors with electronic photodiodes. Clinical trials succeed at restoring some vision, but are limited by the stimulating electrodes. We study the electrode-neuron interface with a focus on the geometrical dependence of the electrode. The functionality of neurons is intimately connected to their branching and curving shape, described by fractal geometry. We examine the morphology of neurons using fractal analysis. The results inform our electrode designs, which are fabricated using top-down lithographic and bottom-up self-assembly techniques. A novel technique for fabricating a fractal electrode is presented. Heating and cooling a film of poly(methyl methacrylate) on a SiO2 substrate causes fractal structures to form on the surface. The geometry of the structures is temperature dependent, producing crystalline branches at lower temperatures and diffusion-limited aggregates at higher temperatures. Subsequent deposition of antimony nanoclusters shows preferred diffusion to the fractal surface features. The dependence of a photodiode's performance on its top contact geometry is explored using modified nodal analysis. The results reinforce the need to balance a low mean semiconductor-metal separation distance with an adequate contact width for low resistance, all while maximizing light input. Future designs will benefit from the spatial voltage maps produced by the simulation. The electric field emanating from an electrode is also dependent on the geometry of the electrode. The Faraday cage effect is exploited to achieve similar electric field responses to traditional electrode shapes. A preliminary study of neural adhesion to SU-8 fractal electrodes is promising. The neuron grows along the electrode even at 90° turns. The role the fractal geometry plays in neuron and electrode functionality is shown to be significant. Continued study of, and experimentation with, new electrode designs is sure to produce exciting possibilities in the future. This dissertation includes previously unpublished co-authored material.

Ce travail se situe dans le cadre d'un projet pluridisciplinaire visant à développer une prothèse visuelle. La technique la plus utilisée actuellement dans de nombreux types de neuroprothèses est basée sur l'excitation par voie électrique via des électrdes. Les inconvénients d'une telle technique (très invasive, de faible résolution spatiale et par contact) pourraient être surmontés en utilisant une stimulation par laser infra-rouge. Nous présentons dans un premier temps les caractéristiques des trois diodes lasers fibrés émettant à 1875 nm, 1535 nm et 1470 nm pour des gammes de puissances optiques de quelques centaines de mW qui ont été utilisés et intégrés à deux dispositifs de mesures permettant l'observations de variations d'échanges ioniques transmembranaires (imagerie de fluorescence des ions calciums et mesure électrophysiologique par la technique de patch clamp). Nous montrons ensuite que des réponses biologiques ont été obtenues par les trois lasers, non seulement sur des cellules ganglionnaires de la rétine et du vestibule de culture mais aussi sur des tranches de rétine. L'influence des paramètres clés comme la longueur d'onde, la durée de stimulation, les seuils d'énergie a été étudié, et a permis d'établir que les seuils d'énergie de stimulation dépendent de la valeur du coefficient d'absorption de l'eau qui varie suivant la longueur d'onde utilisée. Enfin, une étude est consacrée pour expliquer les mécanismes physiques et biologiques apparaissant au cours de l'interaction du laser avec le neurone au niveau cellulaire. Des simulations numériques quantifiant l'élévation de température associées à des tests pharmacologiques cherchant à déterminer la nature des canaux ioniques spécifiques mis en jeu suggèrent la prédominance d'un effet thermique. / This work is part of a pluridisciplinary project, aiming at developing a visual prosthesis. The most used technique for this kind of neuroprosthesis is based on the electrical stimulation of nerves by electrodes. Drawbacks of such a technique (very intrusive, low spatial resolution and physical contact) could be overcome by the use of an infra red laser based stimulation. We present first the three fibre pigtailed laser diode characteristics emitting few hundred of mW at 1875 nm, 1535 nm and 1470 nm. These lasers have been integrated on two measurement devices (a fluorescence microscope and a microscope using patch clamp recording), for the observation of ionic membrane exchanges. Our results show that action potentials have been obtained by laser stimulation from the three lasers, both on retinal or vestibular ganglion cells from mass cultures and on retinal slices. The effect of key parameters as the wavelength, the stimulation time, the energy thresholds has been studied and show that the energy thresholds clearly depend on the absorption coefficient of water which varies with the wavelength. Finally, we present the results of a preliminary study aiming at determining the biophysical interaction mechanisms at cell level. Numerical simulations giving the local increase of temperature and tests of specific blocking molecules in order to know the exact nature of the ionic channels involved suggest a predominant thermal mechanism.

For elderly Canadians, the prevalence of vision impairment caused by degenerative retinal pathologies, such as age-related macular degeneration and retinitis pigmentosa, is at an occurrence rate of 14 percent, and on the rise. It has been shown that visual function can be restored by electrically stimulating intact retinal tissue with an array of micro-electrodes with suitable signals. Commercial retinal implants carrying such a micro-electrode array achieve this, but to date must receive power and data over copper wire cable passing through a permanent surgical incision in the eye wall (sclera). This project is defined by a collaboration with iBIONICS, who are developing retinal implants for treatment of such conditions. iBIONICS has developed the Diamond Eye retinal implant, along with several technology sub-systems to form a comprehensive and viable medical solution. Notably, the Diamond Eye system can be powered wirelessly, with no need for a permanent surgical incision. The thesis work is focused on the formulation, simulation and hardware demonstration of a powering system, mounted on glasses frame, for a retinal implant. The system includes a Micro-Electro-Mechanical System (MEMS) mirror that directs a laser beam to the implant through the pupil opening. The work presented here is built on two main components: an iterative predictor-corrector algorithm (Kalman filter) that estimates pupil coordinates from measurements provided by an image-based eye tracking algorithm; and an misalignment compensation algorithm that maps eye pupil coordinates into mirror coordinates, and compensates for misalignment caused by rigid body motions of the glasses lens mirror and the MEMS mirror with respect to the eye. Pupil tracker and misalignment compensation control performance are illustrated through simulated scenarios. The project also involves the development of a hardware prototype that is used to test algorithms and related software.