<p> </p><p><strong>Abstract</strong></p><p>The focus of this essay is the american director Wes Anderson and the use of colour in his films. I also put some focus on colour as a neglected element in film studies, and what has caused this neglect. In my own research, I have analysed three of Anderson's films: <em>Bottle Rocket</em> (1996), <em>The Royal Tenenbaums</em> (2001) and <em>The Darjeeling Limited</em> (2007). To get a broad sense of a films use of colour, I have investigated the colour scheme, the colours of the costumes, as well as colour patterns. I have also interpreted the symbolism related to certain colours and then used the results of the analyses to answer the question; "how does Wes Anderson use colour, and in which film is this most apparent?" A very generalizing answer to this question, is that Anderson use colour as a means of signifying the characters' feelings and ambitions, and also their relations to the themes of the films. He does this by connecting symbolic values to certain colours and makes these colours stand out in certain scenes and in the characters' clothes. The film that best shows this use of colour is <em>The Royal Tenenbaums</em>. In short, this essay will hopefully bring some understanding of how colours are used in the films of Wes Anderson, but also open up the eyes of the readers to the importance of the neglected element itself; colour.</p><p> </p>

<p>A new European labeling directive (2003/89/EC) states that certain foods and products derived thereof must always be declared. Among the tree nuts specified is Macadamia nut (Macadamia spp.). During the last few years, cases of IgE-allergic reactions, even severe anaphylaxes, have been reported. Reliable methods for the detection of this nut are needed.</p><p>Protein from Macadamia nuts was isolated. Polyacrylamide gel electrophoresis in SDS revealed two main protein bands of about 20 and 50kDa. These protein bands were cut and extracted from the gel and rabbits were immunized with each protein.</p><p>Immunoblotting showed dominant reactivity with the respective antigens. The antisera were further tested for specificity in immunodiffusion and in rocket immunoelectrophoresis.</p><p>In addition, a specific DNA-method was developed, based on Real-Time PCR using Macadamia vicilin as target sequence. Two different primer pairs were tested. Specificity was tested against potentially related nuts. Optimisation of primer and probe concentrations was performed. The limit of detection was 2-4 pg DNA, corresponding to a macadamia nut concentration of 50 to 100 μg per g. In a background of soybean DNA, down to 0,01 % macadamia DNA could be detected.</p>

<p>Reliable methods to analyze food for the presence of almond are important – not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.</p><p>The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate. The ELISA method was about 100-fold more sensitive than rocket immunoelectrophoresis and PCR.</p><p>The specificity of the test kit was evaluated against a number of different nuts and seeds. No important cross reactivity was found. The antibodies against almond used in the kit can not differentiate between almond and apricot kernel. For such purposes the PCR method could be used.</p>

Combustion instability (CI) has been persistent in all forms of propulsion since their inception. CI is characterized by pressure oscillations within the propulsion system. If even a small fraction of the dense energy within the system is converted to acoustic oscillations the system vibrations can be devastating. The coupling of combustion and fluid dynamic phenomena in a nonlinear system poses CI as a significant engineering challenge. Drawing from previous analysis, second order acoustic energy models are taken to third order. Second order analysis predicts exponential growth. The addition of the third order terms capture the nonlinear acoustic phenomena (such as wave steepening) observed in experiments. The analytical framework is derived such that the energy sources and sinks are properly accounted for. The resulting third order solution is compared against a newly performed simplified acoustic closed tube experiment. This experiment provides the interesting result that in a forced system, as the 2nd harmonic is driven, no energy is transferred back into the 1st mode. The subsequent steepened waveform is a summation of 2nd mode harmonics (2, 4, 6, 8...) where all odd modes are nonexistent. The current third order acoustic model recreates the physics as seen in the experiment. Numerical experiments show the sensitivity of the pressure wave limit cycle amplitude to the second order growth rate, highlighting the importance of correctly calculating the growth rates. The sensitivity of the solution to the third order parameter is shown as well. Exponential growth is found if the third order parameter is removed, and increased nonlinear behavior is found if it retained and as it is increased. The solutions sensitivity to this term highlights its importance and shows the need for continued analysis via increasing the models generality by including neglected effects. In addition, the affect of a time varying second order growth rate is shown. This effect shows the importance of modeling the system in time because of the time lag between changes in the growth rate to a change in the limit cycle amplitude.

Combustion instability is a problem that has plagued the development of rocket-propelled devices since their conception. It is characterized by the occurrence of high-frequency nonlinear gas oscillations inside the combustion chamber. This phenomenon degrades system performance and can result in damage to both structure and instrumentation. The goal of this dissertation is to clarify the role of unsteady combustion in the combustor instability problem by providing the first quantified estimates of its effect upon the stability of liquid rocket engines. The combination of this research with a new system energy balance method, accounting for all dynamic interactions within a system, allows for the isolation of combustion effects for this study. These effects are quantified through use of classical linear stability analysis to calculate the unsteady combustion heat release growth rate. Since combustion modeling can become very involved, including the mixing process and multiple reactions concerned, for this initial evaluation the model is limited to a one-dimensional flame analysis for a one-step premixed chemical reaction. Using classical analysis of oscillatory burning, the governing combustion equations are expanded into sets of steady and unsteady equations adapted for premixed liquid rockets. From this expansion process, the first real treatment of the effects of unsteady combustion in a rocket system is presented, and the first quantified values of the unsteady heat release in a rocket system are computed. Finally, the corresponding linear heat release growth rate for the system is then calculated for the first quantified effects of unsteady combustion on the overall system stability. The mechanism of unsteady combustion is shown to behave as a driving mechanism, serving as one of the more important stability mechanisms comparable to the magnitude of the nozzle damping mechanism. This analysis confirms that unsteady combustion is an important stability mechanism that warrants further investigation. This study also creates a firm foundation upon which to extend the analysis of this important mechanism to fully understand all of its effects within a rocket system.

The Earth's mesosphere and lower thermosphere in the altitude range 50-130 km is a fascinating part of our atmosphere. Complex interactions between radiative, dynamical, microphysical and chemical processes give rise to several prominent phenomena, many of those centred around the mesopause region (80-100 km). These phenomena include noctilucent clouds, polar mesosphere summer echoes, the ablation and transformation of meteoric material, and the Earth’s airglow. Strong stratification and small scale interactions are common features of both these phenomena and the mesopause region in general. In order to study interactions on the relevant spatial scales, in situ measurements from sounding rockets are essential for mesospheric research. This thesis presents new measurement techniques and analysis methods for sounding rockets, thus helping to improve our understanding of this remote part of the atmosphere. Considering the need to perform measurements at typical rocket speeds of 1 km/s, particular challenges arise both from the design of selective, sensitive, well-calibrated instruments and from perturbations due to aerodynamic influences. This thesis includes a quantitative aerodynamic analysis of impact and sampling techniques for meteoric particles, revealing a distinct size discrimination due to the particle flow. Optical techniques are investigated for mesospheric ice particle populations, resulting in instrument concepts for accessing smaller particles based on Mie scattering at short ultraviolet wavelengths. Rocket-borne resonance fluorescence measurements of atomic oxygen are critically re-assessed, leading to new calibration concepts based on photometry of O2 airglow emissions. The work presented here also provides important pre-studies for the upcoming PHOCUS rocket campaign from Esrange in July 2010. PHOCUS will address the interaction between three major mesospheric players: meteoric smoke, noctilucent clouds and gas-phase chemistry.

The ability of a propulsion system is very crucial for the capability of a missile or a rocket system. Unlike liquid propellant rocket motors, the only control mechanism of the thrust value is the propellant geometry in solid propellant rocket motors. When the operation of solid propellant rocket motor has started, it cannot be stopped anymore. For this main reason the advance of dual pulse motor technology has started. The aim of this study is to investigate the geometrical effects of design parameters on the flow behavior of a dual pulse solid propellant rocket motor by using commercial Computational Fluid Dynamics (CFD) methods. For the CFD analysis, a generic dual pulse rocket motor model is constituted. Within this model, initially four different geometry alternatives of Pulse Separation Device (PSD) are analyzed. To begin PSD analyses, mesh sensitivity analyses are performed on one PSD geometry alternative. By defined grid size, the analyses of PSD geometry alternatives are performed. Computed results were compared in terms of flow behavior (flow streamlines, velocity distribution, turbulent kinetic energy&hellip / etc.) with each other. With the selected PSD geometry alternative the effects of L/D ratio (Length/Diameter ratio) of first pulse chamber, Achamb/APSD ratio (Chamber area/PSD opening area) and APSD/Ath ratio (PSD opening area/Throat area) on the flow behavior is investigated. Flow analyses are performed by simulating the unsteady flow of second pulse operation. With the performed analyses, it is aimed to identify generic geometric definitions for a dual pulse rocket motor.

Reliable methods to analyze food for the presence of almond are important – not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes. The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate. The ELISA method was about 100-fold more sensitive than rocket immunoelectrophoresis and PCR. The specificity of the test kit was evaluated against a number of different nuts and seeds. No important cross reactivity was found. The antibodies against almond used in the kit can not differentiate between almond and apricot kernel. For such purposes the PCR method could be used.

Food allergy is an IgE-mediated immunological disease, which affects almost 4% of the adult population and up to 6% of children. Proteins from milk, egg, peanuts, soybean, wheat, fish and nuts are the main cause of food allergies. A less common allergen is pea protein. The National Food Administration analyses undeclared pea protein and contaminations of pea protein in foods using rocket immunoelectrophoresis and immunodiffusion. For both methods an antiserum against pea protein is needed. The aim of this study has been to characterize a newly developed rabbit-antiserum against pea protein. It is important to know if the antiserum is specific against peas, the detection as well as the quantification limits before it can be taken into use. The results of the study show that the antiserum was not absolutely specific, since it cross-reacted with chickpeas, fenugreek and lenses. However there is an "in-house" established PCR-method that can distinguish between chickpeas, fenugreek and peas and that method can be used as a complement to the rocket immunoelectrophoresis. The PCR-method cannot be used alone because it is not quantitative. Rocket immunoelectro¬phoresis detects 0,003% pea protein with purified IgG-antibodies from the antiserum.

Abstract The focus of this essay is the american director Wes Anderson and the use of colour in his films. I also put some focus on colour as a neglected element in film studies, and what has caused this neglect. In my own research, I have analysed three of Anderson's films: Bottle Rocket (1996), The Royal Tenenbaums (2001) and The Darjeeling Limited (2007). To get a broad sense of a films use of colour, I have investigated the colour scheme, the colours of the costumes, as well as colour patterns. I have also interpreted the symbolism related to certain colours and then used the results of the analyses to answer the question; "how does Wes Anderson use colour, and in which film is this most apparent?" A very generalizing answer to this question, is that Anderson use colour as a means of signifying the characters' feelings and ambitions, and also their relations to the themes of the films. He does this by connecting symbolic values to certain colours and makes these colours stand out in certain scenes and in the characters' clothes. The film that best shows this use of colour is The Royal Tenenbaums. In short, this essay will hopefully bring some understanding of how colours are used in the films of Wes Anderson, but also open up the eyes of the readers to the importance of the neglected element itself; colour.