No description available.

Thesis (M.A.)--Texas State University-San Marcos, 2006. / Vita. Appendices: leaves 83-122. Includes bibliographical references (leaves 123-136).

Thesis (Ph.D.)(Microbiology and Plant Pathology)--University of Pretoria, 2006. / Includes summary. Includes bibliographical references. Available on the Internet via the World Wide Web.

Thesis (M.A.)--Texas State University-San Marcos, 2006. / Vita. Appendices: leaves 83-122. Includes bibliographical references (leaves 123-136).

Thesis (M.Sc.)--Memorial University of Newfoundland, 2002. / Restricted until May 2003. Bibliography: leaves 108-130.

Please read the abstract on Chapter 5. / Thesis (PhD (Microbiology and Plant Pathology))--University of Pretoria, 2007. / Microbiology and Plant Pathology / unrestricted

Sweet potato single-leaf cuttings of cultivars ‘Centennial,’ ‘Jewel,’ and ‘Nemagold’ were planted in plastic pots containing steam-sterilized sand. Fully opened leaves were counted from the terminal apex on stock plant stems, and leaf positions 1 to 3 and 7 to 9 were referred to as mean leaf positions 2 and 8 respectively. Dry weight and its partitioning among plant organs (leaf, stem, petiole, new shoot, fibrous roots, and stronger roots) differed among the three cultivars. ‘Centennial’ had the highest storage root dry weights in both the first (December-March) and second (April-June) experiments. The new shoots and storage root dry rates of ‘Centennial’ and ‘Jewel’ were 3 to 25 times greater at 79 days after planting (DAP) in the second experiment than at 70 DAp in the first experiment. The new shoot was the dominant sink in ‘Nemagold’ between 28 and 70 DAP in the first experiment but not in the second experiment, and the storage root never became a strong sink in either experiment. A shift from leaf dry weight increases to decrease was observed in the first experiment but not in the second experiment. Leaves in mean leaf position 2 showed higher original plant material (leaf, petiole, and stem) dry weight than mean leaf position 8, but there were no significant differences in final storage root dry weight between these two mean leaf positions in both experiments. Mean leaf positions 2 approximant net assimilation rate (NAR) values based on leaf and new shoot dry weight exceeded mean leaf positions 8 approximate NAR values in both experiments. Plants with stem attachment had higher new shoot and storage root dry weights than plants without stem attachment. Dry weight of the original leaf decreased in plants with steam attachment but not in plants without stem attachment. / M.S.

Orientador: Ana Maria Soares Pereira / Coorientador: Bianca Waléria Bertoni / Banca: Glyn Mara Figueira / Banca: Rosa de Belem das Neves Alves / Banca: Lin Chau Ming / Banca: Elzabeth Orika Ono / Resumo: A espécie Cochlospermum regium foi selecionada por apresentar importantes propriedades terapêuticas relacionadas a infecções do sistema reprodutivo feminino, relatadas em levantamentos etnofarmacológicos. Entretanto, alguns estudos realizados com microorganismos in vitro são contraditórios. Este fato pode estar relacionado a fatores ambientais e fenológicos referentes ao cultivo da planta, a forma de preparação dos extratos e/ou também ao microrganismo alvo. No presente trabalho foram avaliadas a atividade antimicrobiana em raízes de plantas de C. regium coletadas em diversas localidades, estádios fenológicos, idade da planta e condições de sazonalidade. Para realização destes testes antimicrobianos foi desenvolvida uma metodologia a qual utiliza diretamente a droga vegetal sem a necessidade prévia de preparação de extratos (STAMP). Além disso, foram realizados estudos fitoquímicos biomonitorados através da bioautografia. Quanto ao STAMP, constatou-se que este é eficiente para avaliar drogas vegetais principalmente com atividade antifúngica. Com relação aos testes que avaliaram o efeito ambiental e fenológico sobre a atividade antimicrobiana de raízes de C. regium, foi verificado que, apesar de a planta apresentar atividade antimicrobiana em todas as condições avaliadas, foi possível observar que as raízes coletadas no outono e inverno apresentaram maior inibição frente a C. albicans, além disso, a idade da planta e substrato de cultivo, também interferem nesta resposta biológica. Desta maneira, para utilização de extratos desta planta contra essa doença infecciosa causada por C. albicans, recomenda-se a coleta de plantas no outono e inverno. Também foi possível observar que a entrecasca e cerne da raiz são os principais responsáveis pela atividade antimicrobiana. Os estudos fitoquímicos, realizados a partir do extrato hidroalcoólico (80%) ... / Abstract: Cochlospermum regium was selected because it has important therapeutic properties related to infections of female reproductive system, which is reported in ethnopharmacological studies. However, some studies with microorganisms in vitro using this plant are contradictory. This fact could be related to environmental or phenological factors relating to the cultivation of the plant, how to prepare the extracts and/or also the target organism. In this study has been evaluated the antimicrobial activity of C. regium roots collected in different localities, growth stages, plant age and seasonal conditions. To conduct these antimicrobials tests, a methodology has developed which uses the powered plant directly without prior preparation of extracts (STAMP). In addition, biomonitored phytochemical analysis were performed by bioautography assays. Regarding STAMP method, it was efficient for evaluating powered plant mainly with antifungal activity. The tests that evaluated the environmental and phenological effects on the antimicrobial activity of C. regium roots showed that despite the plant antimicrobial activity occur in all conditions evaluated, it has observed that the roots collected in autumn and winter showed greater inhibition against C. albicans. Furthermore, the age of the plant and the substrate of culture also affect the biological response. Thus, we recommend collecting plants in autumn and winter to use against infectious disease caused by C. albicans. It has also observed that both inner bark and root cern are the main responsible for the antimicrobial activity. The phytochemical studies, carried out from the hydroalcoholic extract (80%), showed that the active compounds are of medium polarity, since the highest activity has obtained in the ethyl acetate fraction. Gallic acid was found to be the majority compound 4 in this fraction from GC-MS. Two flavonoids have isolated in ... / Doutor

Made available in DSpace on 2014-06-11T19:32:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-09Bitstream added on 2014-06-13T20:23:51Z : No. of bitstreams: 1 000756284.pdf: 2013969 bytes, checksum: d7866691c0ef92e24e225b1ece791701 (MD5) / A espécie Cochlospermum regium foi selecionada por apresentar importantes propriedades terapêuticas relacionadas a infecções do sistema reprodutivo feminino, relatadas em levantamentos etnofarmacológicos. Entretanto, alguns estudos realizados com microorganismos in vitro são contraditórios. Este fato pode estar relacionado a fatores ambientais e fenológicos referentes ao cultivo da planta, a forma de preparação dos extratos e/ou também ao microrganismo alvo. No presente trabalho foram avaliadas a atividade antimicrobiana em raízes de plantas de C. regium coletadas em diversas localidades, estádios fenológicos, idade da planta e condições de sazonalidade. Para realização destes testes antimicrobianos foi desenvolvida uma metodologia a qual utiliza diretamente a droga vegetal sem a necessidade prévia de preparação de extratos (STAMP). Além disso, foram realizados estudos fitoquímicos biomonitorados através da bioautografia. Quanto ao STAMP, constatou-se que este é eficiente para avaliar drogas vegetais principalmente com atividade antifúngica. Com relação aos testes que avaliaram o efeito ambiental e fenológico sobre a atividade antimicrobiana de raízes de C. regium, foi verificado que, apesar de a planta apresentar atividade antimicrobiana em todas as condições avaliadas, foi possível observar que as raízes coletadas no outono e inverno apresentaram maior inibição frente a C. albicans, além disso, a idade da planta e substrato de cultivo, também interferem nesta resposta biológica. Desta maneira, para utilização de extratos desta planta contra essa doença infecciosa causada por C. albicans, recomenda-se a coleta de plantas no outono e inverno. Também foi possível observar que a entrecasca e cerne da raiz são os principais responsáveis pela atividade antimicrobiana. Os estudos fitoquímicos, realizados a partir do extrato hidroalcoólico (80%)... / Cochlospermum regium was selected because it has important therapeutic properties related to infections of female reproductive system, which is reported in ethnopharmacological studies. However, some studies with microorganisms in vitro using this plant are contradictory. This fact could be related to environmental or phenological factors relating to the cultivation of the plant, how to prepare the extracts and/or also the target organism. In this study has been evaluated the antimicrobial activity of C. regium roots collected in different localities, growth stages, plant age and seasonal conditions. To conduct these antimicrobials tests, a methodology has developed which uses the powered plant directly without prior preparation of extracts (STAMP). In addition, biomonitored phytochemical analysis were performed by bioautography assays. Regarding STAMP method, it was efficient for evaluating powered plant mainly with antifungal activity. The tests that evaluated the environmental and phenological effects on the antimicrobial activity of C. regium roots showed that despite the plant antimicrobial activity occur in all conditions evaluated, it has observed that the roots collected in autumn and winter showed greater inhibition against C. albicans. Furthermore, the age of the plant and the substrate of culture also affect the biological response. Thus, we recommend collecting plants in autumn and winter to use against infectious disease caused by C. albicans. It has also observed that both inner bark and root cern are the main responsible for the antimicrobial activity. The phytochemical studies, carried out from the hydroalcoholic extract (80%), showed that the active compounds are of medium polarity, since the highest activity has obtained in the ethyl acetate fraction. Gallic acid was found to be the majority compound 4 in this fraction from GC-MS. Two flavonoids have isolated in ...

The object of this research was follow up the cumulation of nitrates in individual kinds of vegetables. Analysed was 240 samples of root crops, cole crops and potatoes distributed in the trade network in Humpolec in the years 2008 - 2010. The measurement results show that all the vegetables went beyond the nitrate permitted limit, according to a law n. 53/2002. The most frequently it was in potatoe samples (50 % samples). The next was a carrot (27 %), kohlrabi (25 %), broccoli (24 %) and celery (17 %). From aspect cumulation of nitrates is parsley unchallenged kind of vegetable. Permitted limit was transcendent in 10 % about less than 1 %. The origin of the vegetables was detected as well. Czech republic had the worst results (52,5 %) because of the high nitrate concentration in potatoes. It is grown by a domestic growers. Spain had 20 % in broccoli and kohlrabi. It is imported to our market. Although the high nitrate concentration in some vegetable samples the vegetable influence the human health in a positive way and it doesn?t make health hazard.