Terrestrial target tracking using low Earth orbit satellites provides essential daily services and vital scientific data. In this thesis, the Attitude and Orbit Control System of such a terrestrial tracking satellite, Nanosatellite for Earth Monitoring and Observation Aerosol Monitor, is presented in detail. The satellite is a new generation Earth observation mission with the objective of detecting global atmospheric aerosol content through sub-degree pointing. The design is presented from initial hardware selection and budget development to operation definition and mission operation. The efficacy of performing precise autonomous Earth-pointing on a small satellite platform is validated through high fidelity simulations involving satellite and environmental dynamics, test-characterized hardware models and flight software-in-the-loop. The results provide practical target tracking methodologies which in the past have been publicly inaccessible to the author's best knowledge and which can be now be applied to a broad range of precise Earth-pointing satellites.

This study addresses the design challenges of a proposed Micro Pulse Detonation Engine (MPDE), a micropropulsion system devised to meet the needs of smallsats . It focusses in particular on one subsystem of the proposed MPDE, the explosive delivery system, hereafter refered to as an ejector of granular materials. This study has three major goals: (1) evaluate different ways of building ejectors of granular materials in space, (2) propose a design for an ejector of granular materials that could eventually be used to achieve ejection rates of 10 kHz, and (3) evaluate the new designs performance. We thus begin by reviewing devices that have been built or proposed in the past for ejecting granular materials. Finding them lacking for the objectives of the present project, we evaluate several other different ways of achieving granule ejection in the zero gravity environment of space, based on other previous work with granular materials generally. We conclude that the artificial gravity induced within a spinning apparatus provides for many of the design requirements in a way that none of the other options do. We then go on to discuss the novel challenges a rotating ejector of solids faces, and propose a way of overcoming them. We present the design of the simplest concept we could come up with to achieve controlled ejection from the rotating device. Finally, the performance of this system is evaluated experimentally, using glass ballotini as the granular material. We find that we are able to demonstrate an ejection rate of 50 ejections per second with the device rotating at 10 rotations per second, with the possibility of attaining even higher ejection rates at higher rotational velocities. We conclude that this device demonstrates that there is a very great likelihood that future designs based closely on it will be able to achieve the desired 10 kHz ejection rate, which is the highest ejection rate at which the proposed MPDE can operate while remaining in the higher efficiency vacuum mode.

River ice is an important component of the traditional way of life for the communities along the Slave River both culturally and economically. During the winter, a stable ice cover provides local residents with safe access to their traditional hunting, trapping, and fishing grounds along the river. Periodic spring ice breakup flooding is required to maintain the ecological balance along the Slave River Delta. Recently, however, local observations have indicated changes in ice cover characteristics (e.g. air pocket formation, double layer ice, ice cover flooding) during the winter, which increase the risks of travelling on the ice. Also prolongs dry periods during the spring are leading to rapid growth of invasive vegetation that reduces the lake and channel areas of the Delta. Although some attempts have been made to understand the patterns of spring flood frequency in the Delta, very little is known about the Slave River’s ice cover characteristics and behaviour. Remote sensing techniques and field surveys were used in this study to understand the ice cover progression and to examine ice cover characteristics along the river during the winters of 2013-2014 and 2014-2015. RADARSAT-2 satellite imagery captured the changes in the ice cover and identified different types of ice during the winter seasons at two primary study sites – downstream of Fort Smith and the Slave River Delta. The mechanism of ice cover growth, with the formation of air pockets and layers underneath the ice cover was investigated. Steeper channels and several open water sections appear to be contributing to significant amounts of air entrainment into the water in winter. Changes in the hydraulic characteristics due to flow regulation and ice cover progression can also change the quantity and distribution of air pockets along the river ice cover. Additionally, the impact of flow fluctuations on the ice cover (e.g. ice cover flooding) was also observed. Increases in discharge cause the ice cover to crack or dislodge from the river banks, leading to water seeping onto the ice and flooding it, which has implications for the muskrat and beaver populations. A geospatial model was developed to determine the spatial patterns of ice cover breakup along the river from Fort Fitzgerald to the delta. This model successfully identified the areas of breakup initiation and persistence of ice until the end of the breakup. MODIS satellite imagery was used to describe the temporal patterns and evolution of breakup events between the years 2008 and 2011. In addition to geomorphological influences, air temperature and flow conditions also have strong impacts on the spatial and temporal patterns of the ice cover breakup.

Satellite cells are muscle stem cells that are responsible for the growth and repair of skeletal muscle tissue. Satellite cells typically exist in a quiescent state in their niche between the sarcolemma and basal lamina. In response to muscle tissue injury, activated satellite cells, otherwise known as myoblasts, migrate to the site of injury where they proliferate and subsequently differentiate and fuse to repair damaged myofibers. The success of muscle growth and repair is highly dependent on the speed and degree to which these myoblasts migrate, proliferate and differentiate. This overall process, referred to as myogenesis, is largely controlled by the myogenic regulatory factors, a group of basic helixloop- helix transcription factors including MyoD, Myf5, myogenin and Mrf4. It has recently been found that the Wnt family of secreted signalling proteins are highly involved in the regulation of developmental processes such as myogenesis. Wnt proteins are a family of 21 highly-conserved, secreted, cysteine-rich signalling molecules which are found in all multi-cellular organisms. Wnt signalling is highly versatile and is initiated by the binding of extracellular Wnt to cell-surface Frizzled receptors (Fz). It is highly dependent on both the Wnt isoform and Fz type and may initiate one of three known signalling pathways. Wnt3A and Wnt7A are of particular interest as they have previously been linked with myogenesis. C2C12 myoblasts over-expressing Wnt3A have been seen to have reduced levels of motility and terminal differentiation. Wnt7A is suspected to maintain a healthy satellite cell pool by regulating self-renewal; injection of recombinant Wnt7A into mouse leg muscle resulted in increased satellite cell numbers. In vitro Wnt studies have typically involved the treatment of mouse cells with conditioned medium containing Wnt, often at unknown concentrations. In our study we wished to test the effects of known concentrations of recombinant Wnt3A and Wnt7A on mouse C2C12 and donor-derived human skeletal muscle myoblasts (HSkM) in vitro. Wnt3A and Wnt7A were seen to increase the rate of C2C12 migration in a dose dependent manner. HSkM cells treated with 10 ng/ml Wnt3A also displayed increased motility. Neither Wnt3A nor Wnt7A were seen to have any significant effects on the proliferation of C2C12 or HSkM cells. Wnt3A (10ng/ml and 100 ng/ml) but not Wnt7A was seen to decrease C2C12 terminal differentiation as measured by expression of myosin heavy chain (MyHC). Subsequent confocal microscopy revealed that Wnt3A significantly reduced the percentage of MyoD+ C2C12 nuclei during differentiation. A reduction in nuclear MyoD would support the observed impaired commitment to differentiation. However, donor-derived human skeletal muscle myoblasts treated with 10 ng/ml Wnt3A were not seen to have significantly reduced nuclear MyoD levels or terminal differentiation; the reason for this is unclear but may relate to a number of factors including the concentration of Wnt, Fz and co-receptor profiles and the presence of specific extracellular matrix and serum factors. These studies provide new insight into the role of Wnts in myogenesis and lay the foundation for future work on Wnt3A and Wnt7A. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2014.

A major field of research at the current time is that of implementing Quantum Key Distribution over large distances using satellites. If this protocol works with this technology, it will have huge implications on future information security. In order for a satellite to implement this idea, there are many aspects that must be taken into account. One of the big issues that comes up for this type of system is that of propagating light through the turbulent atmosphere and its effects on the acquisition, pointing and tracking system. The projects studied in this thesis study some of the effects of the atmosphere on certain detectors, try to develop pointing schemes for better accuracy as well as develop knowledge in free space propagation of other single photon experiments. In the first experiment, I study the spatial correlations of the daughter photons created in spontaneous parametric down conversion. I look at the effect of altering the pump beam on the positions of the down converted photons and see if the pump can be manipulated in a way to control the directions of the daughter photons. I begin to utilize a deformable mirror and Shack-Hartmann wavefront sensor which are generally used in adaptive optics, but we plan to use them to alter the pump beam in the spontaneous parametric down conversion process to analyze the correlations between the pump and down converted photons. The second experiment investigates the effects of laser scintillation on the performance of a possible tracking device that could be implemented on a satellite. This quad sensor tracks the position of a beam and a system will be developed to move the sensor to keep the beam in the center where there is a hole for the quantum single photons to stream through. In order to create the effects of scintillation, a turbulence simulator box was built and characterized. This box combines wind turbulence with a heat gradient to mimic atmospheric turbulence on a small scale. Finally, my contributions to a large scale, long distance free space quantum optics experiment are explained and the overall goal of the experiment is discussed. This experiment exposed me to actual free space transmission issues as well as many fundamental techniques for performing long distance optics experiments. In this experiment there was no correction for atmospheric turbulence, but in the future, techniques could be implemented which might increase the efficiencies of the free space links.

Submitted in fulfillment for the Degree of Doctor of Technology: Business Administration, Durban University of Technology, 2014 / Universities are seen to be facing a turbulent storm of challenges, demands, environmental changes, and facilitated thrust in the midst of economic meltdown. The environments in which these institutions are operating are becoming increasingly tempestuous, and competitive which makes leadership crucial as they continuously interface with the local and wider environments. At its best leadership is proximate, contextual and distributed especially in the case of complex organisations like universities. Changes and institutional mergers that took place worldwide two decades ago diffused to the south of Africa as well. These mergers resulted in the formation of mega universities and universities with satellite or branch campuses. If the trend continues with branch campuses spreading even into foreign countries, it will lead to growth and even more leadership complexity. This study was conducted in order to establish the challenges that are faced by staff and students at satellite campuses of universities using the Riverside Campus of the Durban University of Technology as a case study. Literature on leadership and management theories, leadership in higher education and organisational structures was reviewed in order to contextualise the study. A sample of the executive leadership of the university used as a case study was interviewed in order to determine their attitudes towards the current organisational structure. Data was also gathered from the academic and administrative staff at the satellite campus used for the study as well as first and third year students at the same campus using questionnaires. This study is significant because it feeds into the year-long study that was initiated by the Leadership Foundation on Higher Education and conducted from 2010 and finished in July 2011 by the Hull University’s Scarborough Campus that was aimed at determining the most effective relationship between the centre (mother campus) and its satellite campuses, identifying tensions between the strategic and operational levels and making students’ experience consistent. The whole project was undertaken to arrive at a better understanding of the challenges of managing two or more campuses with particular emphasis on strategic matters, organisation, leadership and management structures. The study discovered that there were leadership challenges which were not unique to the university and campus used as a case study, but similar to those identified during literature review. Issues such as lack of leadership and strategic direction, diverse cultures, incomplete merger, isolation, inequitable distribution of resources and lack of development were identified as challenges that needed to be addressed. The study advances as original contribution the ‘Radial Structure’, which was greatly inspired by the natural movement, flow and distribution of energy and information in fractals. This structure ensures equity across all campuses irrespective of size and location. In the radial structure as suggested and implied by the name the corporate division is the source of energy in the form of vision, mission, goals, strategy, and resources which it disburses or radiates equitably across all the campuses. The corporate division is not attached to any campus, but it is a pole that provides an anchor for all the campuses. The energy or feedback is also radiated back to the corporate office from the campuses in the form of student fees, research output from staff and students, pass rates, information to aid decision-making and so on. The relationship that was established between the radial structure and the regression model developed from the responses of the students and theory, was that each campus should be fully-equipped with all the services that the users require for the campus to be fit for purpose. The radial structure would also allow for student services to grow and develop as the campus grows. Like any organisational structure, the radial structure could be adjusted to suit the needs of any university with satellite campuses. The organisational structure would also have to be revisited and adjusted as the needs of the university change. In essence the structure is aimed at ensuring that there is no leadership vacuum at any of the satellite campuses of a university.

Age-related muscle atrophy and the importance of satellite cells in muscle maintenance, growth and repair led us to examine the effects of mechanical stretch, nitric oxide (NO), and age on satellite cell (SC) activation and gene expression in normal young and old mice. Baseline variables (body mass, muscle mass, fiber cross-sectional area (CSA), muscle strength, SC population, stretch activation and gene expression) were obtained from normal C57BL/6 mice at 3-, 8-, 12- and 18-months-of-age. Activation was assayed by 3H-thymidine incorporation into extensor digitorum longus (EDL) muscles isolated for culture. In a second experiment, muscle from 8- and 18-month-old mice was treated with one or more of: stretch; NO-donors (L-Arginine (LA), isosorbide dinitrate (ISDN)) and; Nω-nitro-L-Arginine methyl ester (LN). EDL muscles from 6-month-old mice required a greater stretch stimulus (20% vs. 10% length increase) than EDL from younger mice to increase SC activation. Stretch did not increase SC activation in mice older than 6 months-of-age. NO supplementation from an exogenous source (ISDN) increased SC activation by stretch in 8- but not 18-mo-old EDLs. In a third experiment, 8- and 18-month-old mice were subjected to 3 weeks of voluntary wheel running, or not. The EDL, tibialis anterior (TA), gastrocnemius (GAST) and quadriceps (QUAD) muscles were selected for analysis following sacrifice. The QUAD muscle from 8-month-old mice was the only muscle that demonstrated an exercise-induced increase in SC activation, elevated expression of neuronal nitric oxide synthase (NOS-I) and downregulation of myostatin, a gene that inhibits muscle growth. These results suggest mechanical stimulation of satellite cells and regulation of gene expression that controls muscle growth in voluntary contractile tissue is muscle-specific and age-dependent.

Pax3 and Pax7 belong to a family of conserved transcription factors that play important and diverse roles in development. In the embryo, they carry out similar roles in neural and somite development, but Pax7 fails to compensate for critical functions of Pax3 in the development of limb musculature. Conversely, in the adult, Pax7 is necessary for the maintenance and survival of muscle satellite cells, whereas Pax3 cannot effectively fulfill these roles in the absence of Pax7. To identify the unique roles of Pax7 in adult muscle cells, we have analyzed global binding of Pax3 and Pax7 by ChIP-Seq. Here, we show that despite highly homologous DNA-binding domains, the majority of binding sites are uniquely recognized by Pax7 and are enriched for homeobox motifs. Genes proximal to conserved, unique Pax7 binding sites cluster into specific functional groups which may reflect the unique biological roles of Pax7. Combining Pax7 binding sites with gene expression data, we describe the regulatory networks directed by Pax7 and show that Pax7 binding is associated with positive gene regulation. Moreover, we show Myf5 is a direct target of Pax7 and identify a novel binding site in the satellite cell control region upstream of Myf5.

C/EBPβ is a bZIP transcription factor known to be involved in various physiological processes, including adipogenesis, osteogenesis and liver development. Previous studies in this laboratory revealed an inhibition of myogenesis and reduced myogenic protein expression in 5-azacytidine treated mesenchymal stem cells retrovirally transduced to overexpress C/EBPβ. The goal of this thesis was to evaluate the role of C/EBPβ in myogenic differentiation by overexpression in C2C12 myoblasts and primary myoblasts. We demonstrate reduced MyoD protein expression and subsequent downregulation of myogenic proteins during differentiation following C/EBPβ overexpression. We localized C/EBPβ to the quiescent Pax7+ satellite cells associated with the muscle fiber. Upon satellite cell activation, we observed the downregulation of C/EBPβ protein expression prior to MyoD protein expression. Furthermore, the re-expression of C/EBPβ correlated with the loss of MyoD expression later in differentiation. Histological analysis of C/EBPβ-/- mice revealed smaller fibers and a reduced Pax7+ satellite cell population as compared to control animals. In this thesis, we propose that C/EBPβ is a negative regulator of skeletal muscle differentiation by inhibiting the expression of MyoD, thus impairing proper progression through the myogenic program. In addition, we propose a role for C/EBPβ in the maintenance of undifferentiatied satellite cells.

Instructive signals, whether external or internal, play critical roles in regulating the developmental potency or ability to self-renew of stem cells. External signals may range from secreted growth factors to extracellular matrix proteins found in the stem cell niche. Internal signals include activated signalling cascades and the eventual transcriptional mechanisms they initiate. In either fashion, stem cells are regulated in a complex temporal and context specific manner in order to maintain or maximise their unique characteristics. Previous experiments suggest that Wnt3a plays a role in maintaining the pluripotent state of mouse embryonic stem (mES) cells. However, in the absence of leukemia inhibitory factor (LIF), Wnt signalling is unable to maintain ES cells in the undifferentiated state. This implies that maintaining the pluripotent state of mES cells is not the primary function of canonical Wnt signalling. To further characterize the role of Wnt3a in pluripotency and lineage specification undifferentiated and differentiated mES cells were induced with Wnt3a. Wnt3a induced the formation of a metastable primitive endoderm state and upon subsequent differentiation, the induction of large quantities of visceral endoderm. Furthermore, we determined that the ability of Wnt3a to induce a metastable primitive endoderm state was mediated by the T-box transcription factor Tbx3. Our data demonstrates a novel role for Wnt3a in promoting the interconversion of undifferentiated mES cells into a pluripotent primitive endoderm state. Aging of skeletal muscle tissue is accompanied by fibrosis, atrophy and remodeling all of which negatively affect muscle performance. Whether this reduction in skeletal muscle competency is directly attributed to a resident adult stem cell population called satellite cells remains largely unknown. Here, we undertook an investigation into how age affects the transcriptional profile of satellite cells and their repopulating ability following transplantation. We determined that as satellite cells age, both their regenerative capacity and ability to colonize the satellite cell niche is reduced. Additionally, we identified satellite cell specific transcriptional profiles that differed with respect to age. Therefore, we conclude that intrinsic factors are an important determinant of satellite cell regenerative capacity during the aging process.