Satellite cells are known as the definitive muscle stem cells and are responsible for skeletal muscle maintenance and repair. The capacity of these satellite cells to participate in myogenesis decreases with age and as a result, muscle repair and maintenance in an aging organism is characterized by fibrosis, lipid accumulation and atrophy, a process known as sarcopenia. Recent parabiotic studies have shown that satellite cells with reduced myogenic capability in aging muscle can be rejuvenated to undergo effective myogenesis when exposed to a young environment. Further analysis has suggested that the Wnt family of signaling proteins identified in serum is pivotal in regulating cell fate, proliferation and differentiation, during aging. Wnt3a is known to regulate fibrogenensis, Wnt10b adipogenesis and Wnt7 myogenesis. In the current study, we aim to determine the cytosolic and secreted expression profiles of the three Wnt isoforms, Wnt3a, 7 and 10b, during myogenesis of early and late passage C2C12 myoblasts. We then extend our analysis to determine whether conditioned media could improve the myogenic capacity of late passage cells. Late passage C2C12 cells had elevated Wnt3a cytosolic levels along with reduced differentiation capacity and a rapidly declining Wnt7 levels, in comparison to early passage cells. The elevated Wnt3a suggests an elevated fibrogenic predisposition, whereas the declining Wnt7 cytosolic levels, a decrease in myogenic capacity. Furthermore, analysis of the secreted vs. cytosolic ratio in Wnt7 levels revealed a more rapid decline in late vs. early passage cells during differentiation, supporting the observed decreased myogenic ability. Moreover, late passage cells also showed lower Wnt10b levels compared to early passage cells. This low level of Wnt10b is likely associated with an increase in adipogenic predisposition. The results obtained in the cross-over experiments indicated that conditioned media from early passage cells did not improve the differentiation of late passage cells by the low levels of Myogenin and MHC. However, early passage cells treated with conditioned media from late passage cells surprisingly showed a marginal increase in both Myogenin and MHC levels. Interestingly, cytosolic Wnt3a and 7 in late passage cells treated with ‘young media’ were increased compared to control whereas early passage cells treated with ‘old’ media showed significantly decreased levels of Wnt3a and 7. Furthermore, early passage cells acquired a declining expression when treated with ‘young’ media whereas late passage cells had an increasing level when treated with ‘old’ media. This indicates a possible improvement in differentiation in late passage cells. Taken together, our results support a role for Wnt7 and Wnt10b in promoting myogenesis while Wnt3a may decrease myogenesis. With the increase in passage numbers, the reduced myogenic predisposition is regulated by reduced Wnt10b, 7 and elevated Wnt3a levels, respectively. Moreover, we speculate that the lack of myogenic improvement in the cross-over experiment could be the presence of unknown secreted factors in ‘young’ media that impedes myogenesis. Finally, cell lines are known to be biologically different to primary myoblasts through the accumulation of mutations which could render the cells less sensitive to growth factors. Therefore, it is imperative that the current study is repeated with primary culture myoblasts. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Narwhals (Monodon monoceros) are sentinel species in the Arctic environment and are a vital component for Inuit culture and subsistence. The Arctic is undergoing rapid changes in temperature and sea ice cover and relatively little is known about how this has and will change narwhal foraging behaviour. There are three narwhal populations in the world, the Baffin Bay (BB), Northern Hudson Bay (NHB), and East Greenland (EG) populations; however, foraging behaviour, in terms of dive behaviour and primary dietary components, has really only been investigated in the BB population. Using a combination of stable isotopes, fatty acids, genetic techniques, and satellite tracking technologies I evaluated foraging behaviour in all three of the world’s narwhal populations. I also investigated social structure in the BB population to determine how adaptable narwhals are to a changing and dynamic Arctic environment. Stable isotopes (δ13C and δ15N) and fatty acids are chemical signatures in the tissues of an organism that can provide long-term information on their diet over varying temporal scales depending upon the tissue. Stable isotope analysis in the three narwhal populations found they forage on different primary prey, suggesting narwhal are adaptable in their preferred prey and that there is potential for them to adjust foraging behavior in the face of changing climate. Dietary changes were also assessed over three decades to determine how sea ice changes have affected narwhal foraging for the NHB and BB populations. Dietary changes were evident and can be attributed to changes in sea ice patterns and an altered migratory pathway for narwhals. An understanding of narwhal social structure is also needed to determine how behaviourally flexible narwhal are in diet and site fidelity. Genetic relatedness and dietary signatures from fatty acids were assessed for an entrapped group to determine if individuals that are closely related forage together, which would support a matrilineally driven social structure where females teach their young foraging strategies, and/or travel and forage together. I found no evidence that narwhals form a matrilineal social group, but they may display a fission-fusion structure, which may be an adaptation to patchy prey distribution in the Arctic. Finally, narwhal dive behaviour in all three populations was investigated to determine if dive behaviour could be used to predict diet. Dive differences among populations did correspond with differences in diet, suggesting that narwhals employ specialized foraging strategies. This has repercussions for their ability to adapt to ecosystem changes. Overall, narwhals may be more flexible in terms of their foraging behaviour than previously believed. However, an increased resilience to changing food webs will not be the only predictor of how narwhals will fare in the face of a changing climate; how they respond to increased industrial activities in their preferred habitats, increased predation from southern predators, and increased competition from southern cetaceans and humans alike, will play an equally large role in how they cope with the future.

Turbo codes have been the subject of much research in recent years, producing results very close to the theoretical limit set by Shannon. The codes have been successfully implemented in satellite and video conferencing systems and provision has been made in 3rd generation mobile systems. These codes have not been used for short frame systems due to the delay at the decoder. In this thesis, comprehensive comparisons of the two common decoding algorithms are made, with reference to short frames. The effects of increasing memory size of component codes, frame sizes, utilising puncturing and errors in channel estimation are investigated over AWGN and Rayleigh fading channels. The decoder systems are compared for complexity as well as for equal numbers of iterations. Results show that less complex decoder strategies produce good results for voice quality bit error rates. Investigations are also made into the effects of errors in signal-to-noise ratio estimation at the SOVA turbo decoder, showing this decoding algorithm to be more resilient than Log-MAP decoders in published literature. The decoders are also tested over channels displaying inter-symbol interference. Channels include a time-invariant channel and three ETSI standard time-varying channels simulating indoor, pedestrian and vehicular situations, upgraded for more realistic Doppler effect. To combat these types of channels, a derivative of turbo codes, turbo equalisation is often used. To keep receiver delay to a minimum, decision feedback equalisation is used here. Results show that the combination can produce improvements in decoded results with increasing turbo iterations where ISI is low, but that iterative improvements do not occur under harsh circumstances. The combination produces much superior results compared with codes on their own under even the most extreme circumstances.

No description available.

The aim of this thesis has been summarized as the design of a micro-satellite camera system and its focal plane simulations. The average micro-satellite orbit heights ranges in between 600-850 km and obviously a multipayload satellite brings volume and power restrictions for each payload. In this work, an orbit height of 600 km and a volume of 20&times / 20&times / 30 cm is assumed, since minimizing the payload dimensions increases the probability of the launch. The pixel size and the dimensions of an imaging detector such as charge-coupled device (CCD) have been defined by the useful image area with acceptable aberration limits on the focal plane. In order to predict the minimum pixel size to be used at the focal plane modulation transfer function (MTF), point spread function (PSF), image distortion and aberration simulations have been carried out and detector parameters for the designed camera have been presented.

In this thesis nonlinear control method techniques are investigated to control the attitude of Low Earth Orbit satellites. Nonlinear control methods are compared with linear control methods. Simulations are done using Matlab and Simulink software and BILSAT-1 parameters are used in the simulations. Reaction wheels are used as the actuator.

Geminiviruses (family Geminiviridae) are characterized structurally by twinned (geminate) morphology of virions (ca. 18-30 nm) and genetically by a genome comprising one or two small circular single stranded DNA (ssDNA) molecules and they are responsible for major crop losses worldwide. The genus Begomovirus (type member Bean golden yellow mosaic virus) is the largest genus of the family Geminiviridae. The members of this genus have either monopartite or bipartite genomes. They are transmitted by whiteflies and infect only dicotyledonous plants. DNA β molecules are symptom modulating single-stranded sat-DNA molecules which are associated with certain monopartite begomoviruses. These molecules are around half the size (approximately 1350 nt in length) of their helper viruses and rely on the helper begomovirus for movement in plant tissues, replication and plant-to-plant transmission by the whitefly (Bemisia tabaci). They contribute to production of symptoms and enhance helper virus accumulation in certain hosts. DNA β molecules encode a single gene, called βC1, on the complementary strand which is important for pathogenicity and suppression of post transcriptional gene silencing. In this study the regulation of βC1 gene expression, a host factor interacting with βC1 and its role in the pathogenicity of DNA β are described. Transient expression studies using Nicotiana tabacum plants and GUS as a reporter gene, identified the sequences important for transcription of βC1 from DNA β associated with Cotton leaf curl Multan virus (CLCuMV). A 68 nt fragment (between -139 to -207), which contains a G-box motif was sufficient for DNA β promoter activity. Deletion of this region also led to loss of DNA β replication capacity. Mutation of the G-box, located at 143 nucleotides upstream of the βC1 start codon, resulted in a two to three times reduction in the DNA β promoter activity. This motif was shown to bind specifically to the nuclear factors isolated from tobacco leaf tissues. Histochemical staining of transgenic tobacco plants expressing the gus gene driven by full length DNA β promoter showed phloem specific localisation patterns. It was concluded that a G-box motif is required for binding of host nuclear factors and is necessary for efficient expression of this phloem specific βC1 gene. An ubiquitin-conjugating enzyme, called SlUBC, was retrieved from screening of a tomato cDNA library, using βC1 encoded by DNA β associated with CLCuMV as the bait. The SlUBC was shown to complement yeast deficient in the ubiquitin-conjugating enzyme. It is thought that this enzyme is a key factor in the ubiquitin proteasome pathway, which plays a central role in many eukaryotic cellular processes. The authenticity and specificity of this interaction was confirmed both in vivo, using a bimolecular fluorescence complementation assay, and in vitro. Domain mapping of βC1 showed that a myristoylation-like motif is required for the interaction with SlUBC in the yeast system and induction of DNA β specific symptoms in host plants. Western blot analysis showed that expression of βC1 in transgenic tobacco plants decreased the level of poly-ubiquitinated proteins as compared with wild type plants. However, the level of expression of homologous SlUBC remained stable in these transgenic plants. These results indicated that interaction of βC1 with the SlUBC is required for DNA β specific symptom induction possibly through down-regulation of the host ubiquitin proteasome pathway. Using GFP transgenic N. benthamiana plants, the βC1 encoded by DNA β associated with CLCuMV showed suppression of post transcriptional gene silencing. This protein inhibited both local and systemic silencing. However, the low level of GFP fluorescence and also the results of RNA analysis in patch co-infiltration assay indicated that βC1 is a weak suppressor of local RNA silencing as compared with P19 protein from Tomato bushy stunt virus. A three-way grafting assay and separate patch infiltration assays showed that βC1 interferes with the activity of GFP silencing signal. Mutation of Gly103 in βC1 which was shown to be required for interaction with SlUBC and induction of DNA β specific symptoms in host plants, had no effect on the silencing suppression activity of βC1 protein. This work has provided a new insight into the importance of a G-box motif in expression of βC1 gene of DNA β and also for binding to the host nuclear proteins. In addition, interaction with a host factor, SlUBC, has been shown to be required for induction of DNA β specific symptoms in experimental plants using ToLCV as a helper virus. However, this interaction was not required for silencing suppression activity of βC1. The results of this study can be adapted to determine the mode of pathogenesis and regulation of expression of βC1 in cotton leaf curl disease. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1337164 / Thesis (Ph.D.) - University of Adelaide, School of Agriculture, Food and Wine, 2008

The past years has seen the advent of the availability of high resolution commercial satellite imagery. This study shows that whilst high resolution commercial satellite imagery is capable of producing reasonable spatial data both in quality and cost for use in an urban GIS the challenges of supplying this data commercially is not limited to simply the provision of the imagery. Since a significant amount of work has been done by others to examine and quantify the technical suitability and limitations of high resolution commercial satellite imagery, this study examines the practical limitations and opportunities presented with the arrival of this new spatial data source. In order to do this a number of areas are examined; the historical development of the satellite systems themselves, the business evolution of the owning commercial ventures, Geographical Information Systems (GIS) data and service requirements for a diverse range of spatial data applications and finally the evaluation and comparison of the imagery as a spatial data source. The study shows that high resolution commercial satellite imagery is capable of providing spatial data and imagery for a variety of uses at different levels of accuracy as well as opening up a new era in the supply and application of metric imagery. From a technical approach high resolution commercial satellite imagery provides remote access, one metre or better resolution, 11 bit imagery and a multispectral capability not previously available from space. Equally as challenging is the process or achievement in making the technical capability a reality in a commercial world requiring a financial return at all levels; from the image vendors to the spatial science professional providing a service to a paying customer. The imagery must be financially viable for all concerned.

Geminiviruses (family Geminiviridae) are characterized structurally by twinned (geminate) morphology of virions (ca. 18-30 nm) and genetically by a genome comprising one or two small circular single stranded DNA (ssDNA) molecules and they are responsible for major crop losses worldwide. The genus Begomovirus (type member Bean golden yellow mosaic virus) is the largest genus of the family Geminiviridae. The members of this genus have either monopartite or bipartite genomes. They are transmitted by whiteflies and infect only dicotyledonous plants. DNA β molecules are symptom modulating single-stranded sat-DNA molecules which are associated with certain monopartite begomoviruses. These molecules are around half the size (approximately 1350 nt in length) of their helper viruses and rely on the helper begomovirus for movement in plant tissues, replication and plant-to-plant transmission by the whitefly (Bemisia tabaci). They contribute to production of symptoms and enhance helper virus accumulation in certain hosts. DNA β molecules encode a single gene, called βC1, on the complementary strand which is important for pathogenicity and suppression of post transcriptional gene silencing. In this study the regulation of βC1 gene expression, a host factor interacting with βC1 and its role in the pathogenicity of DNA β are described. Transient expression studies using Nicotiana tabacum plants and GUS as a reporter gene, identified the sequences important for transcription of βC1 from DNA β associated with Cotton leaf curl Multan virus (CLCuMV). A 68 nt fragment (between -139 to -207), which contains a G-box motif was sufficient for DNA β promoter activity. Deletion of this region also led to loss of DNA β replication capacity. Mutation of the G-box, located at 143 nucleotides upstream of the βC1 start codon, resulted in a two to three times reduction in the DNA β promoter activity. This motif was shown to bind specifically to the nuclear factors isolated from tobacco leaf tissues. Histochemical staining of transgenic tobacco plants expressing the gus gene driven by full length DNA β promoter showed phloem specific localisation patterns. It was concluded that a G-box motif is required for binding of host nuclear factors and is necessary for efficient expression of this phloem specific βC1 gene. An ubiquitin-conjugating enzyme, called SlUBC, was retrieved from screening of a tomato cDNA library, using βC1 encoded by DNA β associated with CLCuMV as the bait. The SlUBC was shown to complement yeast deficient in the ubiquitin-conjugating enzyme. It is thought that this enzyme is a key factor in the ubiquitin proteasome pathway, which plays a central role in many eukaryotic cellular processes. The authenticity and specificity of this interaction was confirmed both in vivo, using a bimolecular fluorescence complementation assay, and in vitro. Domain mapping of βC1 showed that a myristoylation-like motif is required for the interaction with SlUBC in the yeast system and induction of DNA β specific symptoms in host plants. Western blot analysis showed that expression of βC1 in transgenic tobacco plants decreased the level of poly-ubiquitinated proteins as compared with wild type plants. However, the level of expression of homologous SlUBC remained stable in these transgenic plants. These results indicated that interaction of βC1 with the SlUBC is required for DNA β specific symptom induction possibly through down-regulation of the host ubiquitin proteasome pathway. Using GFP transgenic N. benthamiana plants, the βC1 encoded by DNA β associated with CLCuMV showed suppression of post transcriptional gene silencing. This protein inhibited both local and systemic silencing. However, the low level of GFP fluorescence and also the results of RNA analysis in patch co-infiltration assay indicated that βC1 is a weak suppressor of local RNA silencing as compared with P19 protein from Tomato bushy stunt virus. A three-way grafting assay and separate patch infiltration assays showed that βC1 interferes with the activity of GFP silencing signal. Mutation of Gly103 in βC1 which was shown to be required for interaction with SlUBC and induction of DNA β specific symptoms in host plants, had no effect on the silencing suppression activity of βC1 protein. This work has provided a new insight into the importance of a G-box motif in expression of βC1 gene of DNA β and also for binding to the host nuclear proteins. In addition, interaction with a host factor, SlUBC, has been shown to be required for induction of DNA β specific symptoms in experimental plants using ToLCV as a helper virus. However, this interaction was not required for silencing suppression activity of βC1. The results of this study can be adapted to determine the mode of pathogenesis and regulation of expression of βC1 in cotton leaf curl disease. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1337164 / Thesis (Ph.D.) - University of Adelaide, School of Agriculture, Food and Wine, 2008

The nesting demographics of sea turtles using beaches within the Barrow, Lowendal, Montebello (B-L-M) island complex on the North West Shelf of Western Australia were examined in the context of their spatial and temporal distribution and potential for exposure to industrially based artificial light sources. The distribution of overnight turtle tracks throughout the island complex confirmed high density nesting of Chelonia mydas (green turtles) on deep, sandy and high energy beaches and Natator depressus (flatback turtles) on deep, sandy and low energy beaches, while Eretmochelys imbricata (hawksbill turtle) tracks were most visible on shallow, sandy beaches adjacent to near shore coral reef habitat. The three species exhibited a summer nesting peak. Hawksbill turtles commenced nesting in September and continued through to January, green turtles commenced in November and decreased in March. Flatback turtles displayed the most constrained nesting season reported to date in Australia with 86% of the animals visits recorded in December and January only. Nesting population sizes estimated for the three species suggest that on a national scale the B-L-M complex is a moderately large green turtle and a large flatback rookery site. The hawksbill rookery is large on an international scale. While none of the green turtle nesting beaches fell within a 1.5 km radius of industrially based artificial light sources an estimated 42% of nesting flatback turtles and 12% of nesting hawksbill turtles were potentially exposed to these light sources. Testing of green turtle and hawksbill hatchling response to different wavelengths of light indicate that hatchlings from the B-L-M region respond to low wavelength much like hatchlings tested in North America (Witherington 1992a). Flatback hatchlings displayed a similar preference for low wavelength light however their responses to discrete light wavelengths between 400 nm and 700 nm suggest that this species may not discriminate well between wavelengths that lie between 450 nm and 550 nm. This response may be related to the rapid attenuation of visible light that occurs in the turbid near shore habitats favoured by this species. Field based arena studies carried out to investigate hatchling behaviour on nesting beaches with light types commonly used in industrial settings found green turtle and flatback hatchlings are significantly attracted to these lights compared to controls. Lights that emit strongly in the low wavelength range (i.e. metal halide and fluorescent) caused hatchling misorientation at lower intensities than the test light that emitted relatively poorly in this range (high pressure sodium vapour). Hawksbill hatchlings tested in situ under the influence of actual oil and gas onshore and offshore facility based lighting were disrupted from the most direct line to the ocean by these light emissions. Emergence fan mapping methods that measure hatchling orientation on nesting beaches were refined and are proposed as an alternative monitoring tool for use on beaches that are logistically difficult to access for large scale experimental orientation studies. The hatchling behaviour was clearly complicated by beach topography and moon phase. Satellite tracking of post nesting female green and hawksbill turtles from North West Shelf rookeries has identified the Western Australian location of migratory corridors and foraging grounds for these species while Scott Reef turtles migrate from their south Timor Sea rookery to Northern Territory waters. Green turtle nesting on Barrow Island and Sandy Island (Scott Reef) forage at feeding grounds 200 – 1000 km from their nesting beaches. Hawksbill turtles nesting at Varanus Island and Rosemary Islands forage at locations 50 – 450 km from their nesting beaches. While all of the nesting beaches within the B-L-M island complex are protected under the Barrow-Montebello Marine Conservation Reserves, the only foraging ground similarly protected is the Northern Territory foraging ground used by Scott Reef green turtles. None of the foraging grounds used by North West Shelf green or hawksbill turtles is currently protected by conservation reserves.