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Studies on the enzyme DNA-dependent RNA polymeraseO'Hanlon, Karen Ann January 1998 (has links)
No description available.
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Image analysis and signal extraction from cDNA microarrays /Bergemann, Tracy L. January 2004 (has links)
Thesis (Ph. D.)--University of Washington, 2004. / Vita. Includes bibliographical references (leaves 155-161).
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Algorithms for ab initio identification and classification of ncRNAs / Algorithmes ab initio pour l'identification et la classification des ARNs non-codantsPlaton, Ludovic 30 January 2019 (has links)
L'identification des ARN non codants (ARNncs) permet d'améliorer notre compréhension de la biologie.Actuellement, les fonctions biologiques d'une grande partie des ARNncs sont connues.Mais il reste d'autre classes à découvrir.L'identification et la classification des ARNncs n'est pas une tâche triviale.Elle dépend de plusieurs types de données hétérogènes (séquence, structure secondaire, interaction avec d'autres composants biologiques, etc.) et nécessite l'utilisation de méthode appropriées.Durant cette thèse, nous avons développé des méthodes basées sur les cartes auto-organisatrice (SOM).Les SOMs nous permettent analyser et de représenter les ARNncs par une carte où la topologie des données est conservée.Nous avons proposé un nouvel algorithme de SOM qui permet d'intégrer plusieurs sources de données sous forme numérique ou sous forme complexe (représenté par des noyaux).Ce nouvel algorithm que nous appelons MSSOM calcule une SOM pour chaque source de données et les combine à l'aide d'une SOM finale.MSSOM calcule pour chaque cluster la meilleur combinaison de sources.Nous avons par ailleurs développer une variante supervisée de SOM qui s'appelle SLSOM.SLSOM classifie les classes connues à l'aide d'un perceptron multicouche et de la sortie d'une SOM.SLSOM intègre également une option de rejet qui lui permet de rejeter les prédictions incertaines et d’identifier de nouvelles classes.Ces méthodes nous ont permis de développer deux nouveaux outils bioinformatique.Le premier est l'application d'une variante de SLSOM pour la discrimination entre les ARNs codants et non-codants.Cet outil que nous appelons IRSOM a été testé sur plusieurs espèce venant de différents règnes (plantes, animales, bactéries et champignons).A l'aide de caractéristique simples, nous avons montré que IRSOM permet de séparer les ARNs codants des non-codants.De plus, avec la visualisation de SOM et l'option de rejet nous avons pu identifier les ARNs ambiguë chez l'humain.Le second s'appelle CRSOM et permet de classifier les ARNncs en différentes sous-classes.CRSOM est une combinaison de MSSOM et SLSOM et utilise deux sources de données qui sont la fréquence des k-mers de séquence et un noyau Gaussien de structure secondaire utilisant la distance d'édition.Nous avons montrer que CRSOM obtient des performances comparable à l'outil de référence (nRC) sans rejet, et de meilleur résultats avec le rejet. / The non-coding RNA (ncRNA) identification helps to improve our comprehension of biology. We know the biological functions for a majority of ncRNA classes. But, we don't know all the classes of ncRNAs. Besides, the identification of ncRNAs using computational methods is not a trivial task. The relevant features for each class of ncRNAs rely on multiple heterogeneous sources of data (sequences, secondary structure, interaction with other biological components, etc.). During this thesis, we developed methods relying on Self-Organizing Maps (SOM).The SOM is used to analyze and represent the ncRNAs by a map of clusters where the topology of the data is preserved.We proposed a new SOM version called MSSOM which can handle multiple sources of data composed of numerical data or complex data (represented by kernels). MSSOM combines data sources by using a SOM for each source and learns the weights of each source at the cluster level.We also proposed a supervised variant of SOM with rejection called SLSOM. SLSOM is able to identify and classify the known classes using multi layer perceptron and the output of a SOM.The rejection options associated to the output layer allow to reject the unreliable prediction and to identify the potential new classes.These methods lead to the development of bioinformatic tools.We applied a variant of SLSOM to the discrimination of coding and non-coding RNAs. This method called IRSOM has been evaluated on a wide range of species coming from different reigns (plants, animals, bacteria and fungi).By using a simple set of sequence features, we showed that IRSOM is able to separate the coding and non-coding RNAs efficiently.With the SOM visualization and the rejection option, we also highlighted and analyzed some ambiguous RNAs on the human. The second one is called CRSOM.CRSOM classify ncRNAs into sub classes by integrating two data sources which are the sequence k-mer frequencies and a Gaussian kernel using the edit distance. We show that CRSOM give comparable results with the reference tool (nRC) without reject and better results with the rejection option.
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Characterisation and evolution of homoimmune Streptomyces bacteriophagesGregory, Matthew Alan January 2000 (has links)
No description available.
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Sequence-specific local structural variations in solution structures of d(CGXX'CG)2 and d(CAXX'TG)2 self-complementary deoxyribonucleic acids.January 1996 (has links)
by Sik Lok Lam. / The "2" in the title is subscript. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 184-197). / ABSTRACT --- p.iii / ACKNOWLEDGEMENTS --- p.v / Chapter CHAPTER ONE: --- LITERATURE SURVEY OF SEQUENCE-SPECIFIC LOCAL STRUCTURAL VARIATIONS IN DEOXYRIBONUCLEIC ACID MOLECULES --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- General Review of DNA --- p.1 / Chapter 1.2.1 --- "Nomenclature, Symbols and Atomic Numbering Scheme of DNA" --- p.2 / Chapter 1.2.2 --- Conformations of DNAs --- p.6 / Chapter 1.2.3 --- Helix-to-Random-Coil Transition --- p.9 / Chapter 1.3 --- Sequence-Specific Local Structural Studies --- p.11 / Chapter 1.4 --- Purpose of This Work --- p.14 / Chapter CHAPTER TWO: --- DETERMINATION OF STRUCTURES OF SOLUTION DNA MOLECULES --- p.17 / Chapter 2.1 --- Introduction --- p.17 / Chapter 2.2 --- Optimization of Conditions --- p.17 / Chapter 2.3 --- Resonance Assignments --- p.19 / Chapter 2.4 --- Extraction of Structural Constraints --- p.22 / Chapter 2.4.1 --- Interproton Distances --- p.23 / Chapter 2.4.2 --- Endocyclic Sugar Torsion Angles --- p.25 / Chapter 2.4.3 --- Phosphate Backbone Torsion Angles --- p.29 / Chapter 2.4.4 --- Hydrogen Bonds --- p.31 / Chapter 2.5 --- Structural Refinement --- p.31 / Chapter CHAPTER THREE: --- SIGNIFICANCE OF DIFFERENT TYPES OF STRUCTURAL CONSTRAINTS IN STRUCTURAL REFINEMENT PROCESS --- p.35 / Chapter 3.1 --- Introduction --- p.35 / Chapter 3.2 --- Experimental --- p.36 / Chapter 3.2.1 --- DNA Model Building --- p.36 / Chapter 3.2.2 --- Generation of Structural Constraints --- p.37 / Chapter 3.2.3 --- Structural Refinement --- p.40 / Chapter 3.3 --- Results and Discussion --- p.41 / Chapter 3.3.1 --- Endocyclic Sugar Torsion Angle Constraints --- p.45 / Chapter 3.3.2 --- Phosphate Backbone Torsion Angle Constraints --- p.49 / Chapter 3.3.3 --- Hydrogen Bond Constraints --- p.50 / Chapter 3.4 --- Summary --- p.50 / Chapter CHAPTER FOUR: --- EFFECTS OF DIFFERENT VARIABLES IN THE RESTRAINED MOLECULAR DYNAMICS PROCESS --- p.52 / Chapter 4.1 --- Introduction --- p.52 / Chapter 4.2 --- Experimental --- p.53 / Chapter 4.3 --- Results and Discussion --- p.55 / Chapter 4.3.1 --- Variables in the Temperature Profile --- p.58 / Chapter 4.3.2 --- Variables in the Force Constant Profile --- p.62 / Chapter 4.4 --- Summary --- p.65 / Chapter CHAPTER FIVE: --- THE J-COUPLING RESTRAINED MOLECULAR MECHANICS PROTOCOL - AN EFFICIENT AND RELIABLE ALTERNATIVE IN DERIVING ENDOCYCLIC SUGAR TORSION ANGLE CONSTRAINTS --- p.66 / Chapter 5.1 --- Introduction --- p.66 / Chapter 5.2 --- Methodology --- p.71 / Chapter 5.2.1 --- "Establishment of the Correlation of 3J1'2, withvi" --- p.71 / Chapter 5.2.2 --- Sample Preparation --- p.73 / Chapter 5.2.3 --- NMR Analysis --- p.73 / Chapter 5.2.4 --- Theoretical Testing of the Protocol --- p.74 / Chapter 5.2.5 --- Experimental Testing of the Protocol --- p.75 / Chapter 5.3 --- Results and Discussion --- p.76 / Chapter 5.3.1 --- Selection of the Appropriate JrMM-derived Torsion Angles --- p.85 / Chapter 5.3.2 --- Theoretical Testing of the Protocol --- p.88 / Chapter 5.3.3 --- Experimental Testing of the Protocol --- p.93 / Chapter 5.4 --- Summary --- p.98 / Chapter CHAPTER SIX: --- HETERONUCLEAR SINGLE QUANTUM COHERENCE DERIVED BACKBONE TORSION ANGLE CONSTRAINTS --- p.99 / Chapter 6.1 --- Introduction --- p.99 / Chapter 6.2 --- Experimental --- p.102 / Chapter 6.3 --- Results and Discussion --- p.103 / Chapter 6.3.1 --- Determination of the Backbone Torsion Angles β and E --- p.103 / Chapter 6.3.2 --- Error Estimation on 3JC4'p- and 3JH3'p-derived E --- p.109 / Chapter 6.4 --- Summary --- p.110 / Chapter CHAPTER SEVEN: --- SOLUTION STRUCTURES OF d(CGXX,CG)2 AND d(CAXX´ةTG)2 --- p.111 / Chapter 7.1 --- Introduction --- p.111 / Chapter 7.2 --- Experimental --- p.111 / Chapter 7.2.1 --- Sample Preparation --- p.112 / Chapter 7.2.2 --- Resonance Assignment --- p.112 / Chapter 7.2.3 --- Melting Profile Study --- p.112 / Chapter 7.2.4 --- Extraction of Structural Constraints --- p.113 / Chapter 7.2.5 --- Structural Refinement --- p.115 / Chapter 7.2.6 --- Structural Parameter Analysis --- p.116 / Chapter 7.3 --- Results and Discussion --- p.116 / Chapter 7.3.1 --- Melting Profile Study --- p.117 / Chapter 7.3.2 --- Structural Constraints --- p.120 / Chapter 7.3.3 --- Structural Refinement --- p.129 / Chapter 7.3.4 --- Structural Features --- p.135 / Chapter CHAPTER EIGHT: --- SEQUENCE-SPECIFIC LOCAL STRUCTURAL STUDY --- p.156 / Chapter 8.1 --- Introduction --- p.156 / Chapter 8.2 --- Predictions from the Calladine's Rules --- p.156 / Chapter 8.3 --- Predictions from Olson's Base-Pair Morphology Dependent Clash Function --- p.160 / Chapter 8.4 --- Re-formulation of Calladine's Idea and its Relationship to Sequence-Specific Local Structural Function ΣLS --- p.163 / Chapter 8.4.1 --- Sequence-Specific Base-Pair Geometry Analysis --- p.164 / Chapter 8.4.2 --- Sequence-Specific Base-Pair Step Geometry Analysis --- p.166 / Chapter 8.4.3 --- Sequence-Specific Local Structural Function ΣLS --- p.167 / Chapter 8.5 --- Summary --- p.173 / Chapter CHAPTER NINE: --- CONCLUSIONS AND FURTHER WORK --- p.174 / APPENDIX I The Base Proton Regions of the lH NMR Spectra of the Hexamers --- p.177 / APPENDIX II 2D NOESY Spectra (Tm = 200 ms) of the Hexamers --- p.178 / "APPENDIX III The H1'-H27H2"" Regions of the DQF-COSY Spectra of the Hexamers" --- p.180 / APPENDIX IV The C4'-H4' Regions of the HSQC Spectra of the Hexamers --- p.182 / REFERENCES --- p.184
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Sensitive forensic DNA analysis : application of pyrosequencing and real-time PCR quantification /Andréasson, Hanna, January 2005 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2005. / Härtill 6 uppsatser.
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Gene expression of adult human heart as revealed by random sequencing of cDNA library.January 1995 (has links)
by Tsui Kwok-wing. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 188-216). / ACKNOWLEDGEMENTS --- p.ii / ABSTRACT --- p.iii / TABLE OF CONTENTS --- p.v / ABBREVIATIONS --- p.ix / Chapter CHAPTER 1 --- INTRODUCTION / Chapter 1.1 --- General introduction --- p.1 / Chapter 1.2 --- Human genome project --- p.5 / Chapter 1.3 --- Organization of human genome --- p.7 / Chapter 1.4 --- Adult human heart cDNA library --- p.9 / Chapter 1.5 --- Gene expression in adult human heart --- p.10 / Chapter 1.6 --- Polymerase chain reaction --- p.12 / Chapter 1.7 --- Purification of PCR products --- p.15 / Chapter 1.8 --- Automated DNA sequencing --- p.17 / Chapter 1.9 --- Sequence analysis by electronic mail server --- p.21 / Chapter 1.10 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerases --- p.23 / Chapter 1.11 --- Transcription factors and zinc finger proteins --- p.25 / Chapter 1.12 --- LIM domain --- p.28 / Chapter 1.13 --- Cysteine-rich intestinal protein --- p.30 / Chapter CHAPTER 2 --- MATERIALS AND METHODS / Chapter 2.1 --- Plating out the adult human heart cDNA library --- p.32 / Chapter 2.2 --- Amplification by polymerase chain reaction --- p.33 / Chapter 2.3 --- Purification of the PCR products by Millipore filters --- p.35 / Chapter 2.4 --- Elimination of the purification of the PCR products before sequencing --- p.36 / Chapter 2.5 --- Cycle sequencing --- p.37 / Chapter 2.6 --- Unicycle sequencing --- p.38 / Chapter 2.7 --- Sequencing by T7 polymerase --- p.39 / Chapter 2.8 --- Gel electrophoresis in the automated A.L.F. sequencer --- p.41 / Chapter 2.9 --- Sequence analysis by commercially available softwares --- p.42 / Chapter 2.10 --- Sequence analysis through electronic mail server --- p.44 / Chapter 2.11 --- Database for storing the result of each clone --- p.46 / Chapter 2.12 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerase --- p.47 / Chapter 2.13 --- Mini-preparation of plasmid DNA --- p.50 / Chapter 2.14 --- Large scale preparation of plasmid DNA --- p.51 / Chapter 2.15 --- Cloning the human cysteine rich heart protein (hCRHP) into the pAED4 vector --- p.53 / Chapter 2.16 --- Expression of hCRHP in E coli --- p.56 / Chapter 2.17 --- Northern hybridization --- p.58 / Chapter 2.18 --- Partial protein sequencing of hCRHP --- p.59 / Chapter CHAPTER 3 --- RESULTS / Chapter 3.1 --- The sequencing results of adult human heart cDNA clones --- p.60 / Chapter 3.2 --- Accuracy of sequencing results --- p.63 / Chapter 3.3 --- Catalogues of genes expressed in the adult human heart --- p.65 / Chapter 3.4 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerases --- p.94 / Chapter 3.5 --- Elimination of the purification of the PCR products before sequencing --- p.102 / Chapter 3.6 --- Sequence analysis of hCRHP --- p.104 / Chapter 3.7 --- Northern hybridization of hCRHP --- p.109 / Chapter 3.8 --- Expression of hCRHP in E. coli --- p.112 / Chapter CHAPTER 4 --- DISCUSSION / Chapter 4.1 --- Random sequencing of adult human heart cDNA clones --- p.118 / Chapter 4.2 --- Catalogues of genes expressed in the adult human heart --- p.130 / Chapter 4.3 --- Gene expression in the adult human heart --- p.137 / Chapter 4.4 --- Importance of nonhuman matches --- p.170 / Chapter 4.5 --- Effects of agar and agarose on Vent´ёØ and Taq DNA polymerases --- p.177 / Chapter 4.6 --- Elimination of the purification of the PCR products before sequencing --- p.180 / Chapter 4.7 --- The possible role of CRIP and hCRHP --- p.184 / Chapter 4.8 --- Future prospect --- p.186 / REFERENCE --- p.188
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Identification of human cytosolic malate dehydrogenase by large scale human heart cDNA library sequencing.January 1995 (has links)
by Agnes, Lo Shuk Yee. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves [27-33] (2nd gp.)). / Chapter PART 1: --- Human Heart cDNA Library Sequencing / Chapter A) --- Introduction of human heart cDNA library sequencing / Chapter A.1 --- Human genome project / Chapter A.2 --- The aim of human genome project / Chapter A.3 --- Automatic sequencing / Chapter A.4 --- Cycle sequencing reaction / Chapter A.5 --- Human heart cDNA library sequencing project / Chapter B) --- Methods and materials / Chapter (I) --- Preparation of plating bacterial-Y1090 / Chapter (II) --- Plating the bacteriophage with blue-white visual selection / Chapter (III) --- Amplification of bacteriophage cDNA clones by PCR / Chapter (IV) --- Purification and quantitation of PCR products / Chapter (V) --- Cycle DNA sequencing of PCR products / Chapter (VI) --- Casting the sequencing gel / Chapter (VII) --- Sequencing by Pharmacia LKB A.L.F. DNA Sequencer / Chapter (VIII) --- Editing and saving the DNA sequence / Chapter (IX) --- Sending the DNA sequence to Genbank by E-mail / Chapter (X) --- Usage of the Genbank database / Chapter C) --- Results / Chapter D) --- Discussions / Chapter D.1 --- Application of human genomic project / Chapter D.2 --- Interpretation of the sequencing results / Chapter D.3 --- Quality of cDNA libraries and representation of mRNA population / Chapter D.4 --- "Gene expression profile in three different organs-heart, brain and liver" / Chapter D.5 --- Population study of the cDNA library / Chapter D.6 --- Isolation of a large number of novel genes by substraction cDNA library / Chapter D.7 --- Screening method to find out the complete coding sequence of interesting genes / Chapter D.8 --- Technical problems encountered and managed / Chapter PART 2: --- Identification of human cytosolic malate dehydrogenase by large scale human heart cDNA library sequencing / Chapter CHAPTER 1: --- Introduction of malate dehydrogenase / Chapter 1.1 --- Malate dehydrogenase--Kreb's cycle enzyme / Chapter 1.2 --- Two stereospecific forms of dehydrogenase / Chapter 1.3 --- NAD-binding domain / Chapter 1.4 --- The active site / Chapter 1.5 --- Comparison of surface properties between cMDH and mMDH / Chapter 1.6 --- N-terminal region and mitochondrial import / Chapter 1.7 --- Subunit-subunit interactions / Chapter 1.8 --- Physiological importance of malate dehydrogenase / Chapter 1.9 --- Secondary structure-total 11 β-strands and 9 α-helixes / Chapter 1.10 --- Objectives of the thesis / Chapter CHAPTER 2: --- Cloning and sequence analysis of human cytosolic malate dehydrogenase (hcMDH) / Chapter 2.1 --- Cloning of human cytosolic malate dehydrogenase (hcMDH) / Chapter 2.1.1 --- Methods and materials / Chapter 2.1.1.1 --- Cloning full length of hcMDH into expression vector pAED4 / Chapter 2.1.1.2 --- Preparation of competent cell-JM109 for transformation / Chapter 2.1.1.3 --- Minipreparation of plasmid DNA / Chapter 2.1.1.4 --- Midi-preparation of bacteriophage λDNA by QIAGEN´ёØ / Chapter 2.1.1.5 --- Titration of bacteriophage λ of human adult heart cDNA library / Chapter 2.1.1.6 --- Preparation of soft-agarose lysates / Chapter 2.1.1.7 --- Elution of DNA from agarose gel by GENECLEAN´ёØ / Chapter 2.1.2 --- Results / Chapter 2.1.3 --- Discussions / Chapter 2.2 --- Sequence analysis of human cytosolic malate dehydrogenase (hcMDH) / Chapter 2.2.1 --- Methods and materials: Autoread sequencing / Chapter (I) --- Annealing of primer to double-stranded template / Chapter (II) --- Sequencing / Chapter 2.2.2 --- Results and discussions / Chapter 2.3 --- Amino acids and protein structure analysis of cMDH / Chapter CHAPTER 3 : --- "Protein expression, partial purification and folding experiments of human cytosolic malate dehydrogenase (hcMDH)" / Chapter 3.1 --- Protein expression of hcMDH in E. coli / Chapter 3.1.1 --- Methods and materials / Chapter 3.1.1.1 --- Protein expression induced by IPTG / Chapter 3.1.1.2 --- Isoelectric focusing (IEF)-two dimensional gel electrophoresis / Chapter (I) --- First dimensional electrofocusing / Chapter (II) --- The second dimension SDS-PAGE electrophoresis / Chapter (III) --- Sample preparation / Chapter 3.1.2 --- Results / Chapter 3.1.3 --- Discussions / Chapter 3.1.3.1 --- The properties of expressed protein of hcMDH / Chapter 3.1.3.2 --- T7 expression system / Chapter 3.1.3.3 --- Strong φ 10 promoter / Chapter 3.1.3.4 --- E.coli BL21 host cell / Chapter 3.2 --- Partial purification and folding experiments of hcMDH / Chapter 3.2.1 --- Methods and materials / Chapter 3.2.1.1 --- Partial purification of hcMDH expressed protein / Chapter (I) --- Preparation of supernatant from E.coli crude extract / Chapter (II) --- Ion-exchange column chromatography / Chapter (III) --- Affinity chromatography / Chapter (IV) --- Gel filtration on a Sepharose CL-6B column / Chapter 3.2.1.2 --- Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) / Chapter 3.2.1.3 --- Staining the protein gel by the Coomassie Blue R-250 method / Chapter 3.2.1.4 --- Staining the protein gel by the Silver staining Method / Chapter 3.2.1.5 --- Quantitation of protein by the Bradford Method / Chapter 3.2.1.6 --- Native gel electrophoresis / Chapter 3.2.1.7 --- Malate dehydrogenase MDH enzyme staining method / Chapter 3.2.1.8 --- Malate dehydrogenase MDH enzyme assay / Chapter 3.2.1.9 --- Fast protein liquid chromatography (FPLC) / Chapter 3.2.1.10 --- Protein folding experiment / Chapter 3.2.1.11 --- Eukaryotic expression of hcMDH / Chapter 3.2.2 --- Results / Chapter 3.2.2.1 --- Partial purification by chromatography / Chapter 3.2.2.2 --- Native gel / Chapter 3.2.2.3 --- FPLC / Chapter 3.2.2.4 --- To aid folding of protein by adding NADH / Chapter 3.2.2.5 --- Eukaryotic expression / Chapter 3.2.3 --- Discussions / Chapter 3.2.3.1 --- Purification of malate dehydrogenase MDH / Chapter 3.2.3.2 --- "Methods for visualizing dehydrogenase enzymes, e.g. malate dehydrogenase" / Chapter 3.2.3.3 --- The presence of unfold hcMDH protein in bacteria / Chapter 3.2.3.4 --- Folding of protein by heat shock protein GroE / Chapter 3.2.3.5 --- Eukaryotic expression / Chapter CHAPTER 4: --- Master screening of single base change by PCR-SSCP (Single Strand Conformational Polymorphism) / Chapter 4.1 --- Theory of SSCP / Chapter 4.2 --- Methods and materials / Chapter 4.3 --- Results / Chapter 4.4 --- Discussions / Chapter 4.4.1 --- The procedure of SSCP / Chapter 4.4.2 --- An alternative quick detection method for polymorphism of hcMDH at position 565--by automatic sequencing / Chapter 4.4.3 --- Other detection methods-- RNA-PCR and ddF / Chapter 4.4.4 --- Parameters affecting sensitivity of SSCP / Chapter 4.4.5 --- Application of SSCP / Chapter CHAPTER 5: --- Southern hybridization and In situ hybridization / Chapter 5.1 --- Southern blot analysis of human cytosolic malate dehydrogenase (hcMDH) / Chapter 5.1.1 --- Methods and materials / Chapter (I) --- Transfer genomic DNA to Nylon membrane / Chapter (II) --- Synthesis of radiolabelling cDNA probe / Chapter (III) --- Pre-hybridization and hybridization reaction / Chapter 5.1.2 --- Results / Chapter 5.1.3 --- Discussions / Chapter 5.2 --- In situ hybridization / Chapter 5.2.1 --- Methods and materials / Chapter (I) --- Preparation of Dig labelling probe by random primed labelling / Chapter (II) --- Estimating the yield of Dig-labelled nucleic acids / Chapter (III) --- Denaturation and hybridization of the hcMDH probe with animal tissues / Chapter (IV) --- Color development of the tissue / Chapter 5.2.2 --- Results / Chapter 5.2.3 --- Discussions / Chapter 5.2.3.1 --- Cellular distribution of hcMDH / Chapter 5.2.3.2 --- The principle of in situ hybridization / Chapter 5.2.3.3 --- Specimen preparation / Chapter 5.2.3.4 --- Hybridization conditions / Chapter 5.2.3.5 --- "Ontogeny of MDH in rabbit fetal brain, heart and lung" / Appendixes: / "Appendix I: 531 random cDNA clones from clone no. J950 to K951 in human heart cDNA library sequencing project. The name of clones, accession number, the length of the partial sequence and percentage of match are listed" / Appendix II: The new accession no. of Novel clones in Genbank / "Appendix III: The enzymatic reaction, molecular weigth, specific activity and Michaelis constants of different sources of malate dehydrogenase" / Appendix IV: The full sequence of nucleic acids and amino acids of human cytosolic malate dehydrogenase hcMDH. Accession no. of hcMDH is U20352 in Genbank / Appendix V: Nucleotide sequences of the mouse cMDH gene / Appendix VI: Nucleotide sequences of the mouse mMDH gene / Appendix VII: Structural organization of the mouse cytosolic malate dehydrogenase and its comparison with that of the mouse mitochondrial malate dehydrogenase gene
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Molecular cloning and protein characterization of the developmentally regulated human 1433 epsilon isoform. / CUHK electronic theses & dissertations collectionJanuary 1997 (has links)
by Sharon, Chui-Wah Luk. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (p. 128-146). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web.
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Novel RNA and protein sequences involved in dimerization and packaging of HIV-1 genomic RNARussell, Rodney S. January 2004 (has links)
No description available.
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