Diatoms are photosynthetic protists that reside in aquatic environments. A unique feature of diatoms is their cell wall structure made of silica. Silicon is a limiting nutrient for diatoms. The bioavailable form of silicon in aquatic environments is silicic acid. Since the intracellular concentration of silicon is many times greater than the extracellular concentration in aquatic habitats, silicon transporters (SIT) are required that actively transports silicic acid into the cell. In the diatom Chaetoceros affinis two such transporters have been reported, SIT1 (CaSIT1) and SIT2 (CaSIT2). Gene expression of CaSIT1 is increased at low extracellular concentration of silicic acid (<30 µM), while at higher concentrations the transportation is dominated by diffusion. On the contrary, CaSIT2 does not seem to be affected by variations in environmental silicic acid concentrations, and its role in the transportation of silicic acid remains unclear. The aim of this study was to evaluate the relative gene expression of CaSIT1 and CaSIT2 in C. affinis, using realtime-PCR. The diatom was cultivated in two media: one with full nutrients (control) and one without added silicon (-Si). In addition, C. affinis was also cultivated with a second diatom, Cylindrotheca fusiformis, in respective media (mixed cultivation). Due to extensive primer-dimers, CaSIT2 was excluded from the proceeding analysis of the samples. Analysis showed that the relative gene expression of CaSIT1 was increased by silica limitation (ANOVA: p < 0,05), in accordance with previous studies. The effect of competition however resulted in a decrease of gene expression (ANOVA: p < 0,05). The combined effect, the interaction of competition and silica limitation, did not result in a significant change of the gene expression (ANOVA: p = 0,38). However, there was an increased variation among the mixed cultures, which could be a result of RNA degradation considering the poor RNA quality of the samples. The results from this study show that realtime-PCR is an effective method to measure gene expression of silica transporters, provided that the primers have been correctly evaluated. / Diatomer är vattenlevande, fotosyntetiska protister som kännetecknas av att dess cellväggsstruktur är uppbyggd av kiseldioxid. Kisel är ett begränsande näringsämne för diatomer. Den biotillgängliga formen av kisel i akvatisk miljö är kiselsyra. Eftersom den intracellulära koncentration av kisel är mångfaldigt högre än den extracellulära i de flesta akvatiska habitat, krävs kiseltransportörer (SIT) som aktivt pumpar in kiselsyran. Hos diatomen Chaetoceros affinis har två kiseltransportörer rapporterats, SIT1 (CaSIT1) och SIT2 (CaSIT2). Genuttrycket av CaSIT1 ökar vid låg koncentration av extracellulär kiselsyra (<30 µM), medan vid högre koncentrationer domineras transporten av diffusion. I motsats tycks CaSIT2 inte påverkas av variationer i tillgänglig kiselsyra, och dess roll vid transport av kiselsyra är oklar. Syftet med föreliggande arbete var att med realtids-PCR undersöka det relativa genuttrycket av CaSIT1 och CaSIT2 hos C. affinis. Diatomen odlades i två medium: en med komplett näringsinnehåll (kontroll) och en med samma näringsinnehåll förutom kisel (-Si). Därutöver odlades även C. affinis tillsammans med Cylindrotheca fusiformis, i respektive medium (mixad kultur). På grund av omfattande primer-dimers uteslöts CaSIT2 primers från analys av proverna. Det relativa genuttrycket av CaSIT1 ökade vid kiselbegränsning (ANOVA: p < 0,05), i enlighet med tidigare studier. Effekten av konkurrens resulterade däremot i en minskning av genuttryck (ANOVA: p < 0,05). Den kombinerade effekten (konkurrens och kiselbegränsning) resulterade inte i en signifikant förändring av genuttrycket (ANOVA: p = 0,38). Det var en ökad variation bland de mixade kulturerna, vilket eventuellt förklaras av degraderat RNA då RNA-kvalitén över lag var väldigt låg för de flesta prover. Resultaten i denna studie visar att realtids-PCR är en effektiv metod för att undersöka genuttryck av kiseltransportörgener, under förutsättning av primers utvärderats korrekt.

The subject of this thesis is the Sit-Down Strike in Flint, Michigan in 1936-1937. The main purpose is to examine the story of the strike as told by the strikers themselves, to explore the role that Communists played in the strike along with how the workers responded the Communism and other political ideologies of the day. The final chapter then examines the many anti-Communist forces that surrounded the autoworkers before, during, and after the Sit-Down Strike, which may account for the strikers' reluctance to admit their affiliation with the Communists.

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One very common axiom amongst Strength and Conditioning Professionals and Athletic Performance Coaches is that the least flexible athletes usually produce the fastest 40-yard dash times. Flexibility, in this case specifically refers to posterior chain flexibility (PCF). This is usually measured by athletes ability to perform a sit-and-reach test. This train of thought has been widely accepted within the human performance professions, even though it is void of any scientific investigation or measured validity. The purpose of this study is to determine if there is a relationship between posterior chain flexibility using the sit-and-reach test and speed in the 40-yard dash. All test subjects were male between the ages of 18-22, and members of a Division I university football team. All 95 test subjects had participated in at least one semester of a strength and conditioning program designed by Certified Strength and Conditioning Specialists. Similar of the study done by Johnson (2001), each athlete was placed in a group based on position, line of scrimmage groups (LOS), Skill group (receivers, cornerbacks, rover linebackers, whip linebackers, and safeties), Combo group (inside linebackers, tight ends, quarterbacks, tailbacks, fullbacks, and defensive ends), Specialist group (place- kickers, kick-off specialists, holders, and punters). Each test subject was tested in the 40-yard dash, the sit-and-reach flexibility test, as well as other tests for strength and power such as the bench press, front squat, push jerk, power clean, vertical jump, and agility tests. Body weight and height was also measured. This study used a simple linear regression on the data where the Sit-and-Reach test results were the dependent variable and the subjects timed results in a 40 yard dash was the independent variable. There appears to be no significant relationship between a low flexibility score and the sprint speed of an athlete for the entire group population. The results also show that there is no significant relationship between flexibility of the posterior chain and linear speed as measured in the 40-yard dash when looking at football players that fall under the Combo, Skill, or LOS position groups. There was however a significant relationship for the specialist group. The theory that the least flexible athletes usually produce the fastest 40-yard dash times is not true for those specific groups. / Ph. D.

The brown marmorated stink bug (BMSB), Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), is an invasive phytophagous species native to eastern Asia. Due to its wide host plant range, it is considered among the most harmful agricultural pests in the invaded areas, including Europe, North and South America. In addition to its impact on crops, this insect causes a nuisance in urban areas, by invading buildings for overwintering using their aggregation pheromones and emitting an unpleasant smell when disturbed. The current management of this species is mainly based on the use of chemical insecticides, which show efficacy when applied frequently, with consequent negative impact on beneficial insects in the agroecosystems. As a result, there is a desire for more environmentally friendly solutions for the management of BMSB. A valid alternative is the use of exotic and coevolved biocontrol agents, for instance, the Samurai wasp, Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), which is already showing promising medium and long-term results against BMSB in classical biocontrol projects. Within the frame of the least-toxic alternatives, the use of the Sterile Insect Technique (SIT) in an Area-Wide management approach is potentially a valid strategy to be included in integrated control and eradication programs. The main objective of this research, therefore, was to develop a SIT for potential use in the eradication of BMSB in newly introduced areas and/or as an additional tool to reduce its impact in areas where invasive populations have been already established. However, the effective use of SIT requires producing a high number of insects, and mass rearing for such a species is considered too complicated and expensive. To overcome this limitation, we evaluated a harvest, irradiation, and release strategy using wild overwintering adults. New live traps baited with high-load pheromones have been developed for mass trapping, exploiting the natural phenomenon ruled behaviour of aggregation of BMSB in preparation for overwintering. The captures of the new pheromone traps were compared with those of identically baited sticky panels through daily services lasting 2 weeks. Following winter diapause, the collected males were irradiated with high-energy photons at four different dosages: 16, 24, 32 and 40 Gy. The effects of irradiation on the biology i.e., fertility, longevity, and mating behaviour of overwintering BMSB males were evaluated with respect to unirradiated males. To encourage the use of SIT, a linear accelerator from a hospital facility was used for irradiation instead of the classical method involving radioactive materials. A second control 0 Gy was adopted to evaluate the possible negative effects on the biology (longevity, fertility, and fecundity) of insects due to the preparation for the irradiation. Moreover, as part of an integrated pest management (IPM) strategy, the combined use of the egg parasitoid T. japonicus and SIT was considered. The suitability of sterile eggs as oviposition substrate for T. japonicus was evaluated by comparing irradiated BMSB eggs at 40 Gy, eggs obtained by fertile female mated with sterile males irradiated at 50 Gy, and refrigerated sterile eggs currently used for laboratory rearing and field monitoring. The results of the research are encouraging for SIT applications in IPM approach. 1) The new traps caught up to 15-times more adult BMSB than the sticky panels in the two weeks of collections. 2) The developed irradiation protocol that involved a 6 MV medical linear accelerator has allowed the achievement of a high level of sterility of overwintering males (over 95%) already at 32 Gy without compromising males’ longevity and mating behaviour (in no-choice conditions). No adverse effects due to the irradiation preparation protocol were observed. 3) Sterile eggs obtained by mating sterile irradiated males and fertile females (SIT eggs), showed a higher parasitoid emergence rate (%) than both those directly irradiated and refrigerated. The difference in the emergence rate is even more evident when the eggs are 20 days old, with a parasitoid emergence rate of (75.56 %) for SIT eggs compared to those irradiated (44.68 %) and refrigerated (37.57%). In conclusion: 1) the new live traps are effective in mass trapping, 2) wild harvested males of BMSB can be used in SIT, 3) the use of linear accelerators for irradiation of BMSB is a feasible alternative to classical radioactive sources and 4) sterile eggs obtained by mating sterile males and fertile females are a valid substrate for T. japonicus oviposition, supporting the potential use of SIT on BMSB.

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Osteoarthritis (OA) of the knee is common in Western society. OA of the patellofemoral (PF) compartment of the knee is prevalent in adults greater than 55 years of age. Isolated radiographic PFOA is present in 13.6-24% of females and 11-15.4% of males with knee pain. Biomechanical factors such as tibiofemoral alignment and high joint stress are associated with the development and progression of PFOA. PF joint stress is high when the quadriceps contracts with the knee in a position of extreme flexion, such as rising from sitting. The purposes of this study were to determine 1) the triplanar biomechanics of the hips and knees during sit-to-stand (STS) for persons with PFOA versus age- and gender-matched control subjects, 2) the impact of PFOA on physical performance, perceived functional status, and pain, and 3) the relationship between knee kinematics during STS and physical performance. The biomechanics of STS was examined using a video-based motion analysis system and two force plates. Physical performance was measured with the Timed Up and Go (TUG) and Fifty-Foot Walk (FFW) tests. Perceived functional status, pain, and stiffness were measured using the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) questionnaire. Knee pain was measured with a Visual Analog Scale (VAS) following STS, TUG, and FFW. Persons with PFOA required a significantly longer time to perform STS and the TUG as compared to healthy controls. The PFOA group demonstrated greater hip flexion and knee abduction versus the control group. The hip and knee moments were significantly different with the PFOA group demonstrating greater hip extension, hip abduction, hip external rotation, knee extension, and knee adduction moments. Persons with PFOA were found to have significantly less perceived physical function, greater stiffness, and greater pain. Pain following STS, TUG, and FFW were all greater in the PFOA group. No significant association was found between any knee angle and time to perform the TUG or the FFW. These results indicate that dynamic malalignment of the TF joint is present during STS in persons with PFOA. This may contribute to the increased pain and decreased function in persons with PFOA. / Physical Therapy