This Master Thesis is focused on the concept of outsourcing and Shared Service Center (SSC) as a tool for reducing operational costs. Outsourcing will be defined along with the various types with advantages and disadvantages of each. Further, the work is focused on process of establishing SSC. The main objective of this thesis is to identify and assess the most important factors for establishing a Shared Services Center. The primary target is divided into the sub- goals. These goals include identifying key factors for locating SSC, assessing the financial benefit of establishing SSC and revelation in which parts of the company savings are highest. Next sub-objective is to evaluate various stages of implementation and progress. The last objective is to evaluate using KPIs as a tool for measuring effectiveness of SSC. For achieving these goals online research, evaluation interviews with executives of Vodafone and internal materials are used. For evaluating of locality are also used methods of managerial decision-making. Theoretical part is followed by a case study. I chose an example of establishing Vodafone SSC in Budapest. On this example my knowledge

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No. of bitstreams: 2 Dissertação - Mayara Stefany da Silva Mariano Feitosa - 2017.pdf: 5065674 bytes, checksum: 310307efca7b4a8f1475e4e8accf0214 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-09-29 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Sugarcane (Saccharum spp.) is one of the most important crops in tropical and subtropical regions of the world. It is cultivated in more than 100 countries, where it is used as raw material to obtain sugar and bioethanol. Given its importance, many efforts have been carried out to characterize the genome of sugarcane cultivars. The eukaryotic genomes are confined in different cellular compartments that present different inheritance patterns. Plastids and mitochondria have their own genetic system, comprising DNA, RNA and all the demanded components for replication, transcription and protein synthesis, that occur inside these organelles. The primary function of chloroplasts and mitochondria is energy transduction. Chloroplasts are responsible to convert light into chemical energy during photosynthesis, while mitochondria provides energy to the cell in form of ATP molecules during respiration. In this work, the chloroplast and mitochondrial genomes of sugarcane cultivar RB867515 are assembled and characterized, using data from two next generation sequencing technologies – Illumina and PacBio. In chloroplasts, we sought to identify evidences of heteroplasmy, by using long reads from PacBio technology in the assembly process. In mitochondria, we investigated the occurrence of genetic and structural genomic variations. The assemblies were carried out using screened reads for the organellar genomes. These reads were selected by mapping whole genome shotgun reads to reference genome sequences of chloroplast and mitochondria, that are publicly available. The organellar reads were assembled using SPAdes and Organelle_PBA. Gene annotation was obtained using DOGMA, GeSeq and Mitofy tools. Two chloroplast haplotypes (isoforms) were identified in the cultivar RB867515. These isoforms are different from each other because they present a distinct orientation of the SSC (small single copy) region, confirming the hypothesis of chloroplast heteroplasmy in sugarcane. The genome of each chloroplast isoform comprises 141,181 bp, and shows a typic quadripartite structure, that includes a long single copy region (LSC) of 83,047 bp, which is flanked by two inverted repeat regions (IRs) of 22,795 bp and a small-single copy region (SSC), between IRs, of 12,544 bp. The assembled mitochondrial genome comprised two chromosomes of 300,765 bp and 194,383 bp. The estimates of GC (~44%) and AT (~56%) contents were similar to those obtained for other angiosperms. A total of 39 CDSs, 5hypothetical conserved genes, 5 rRNAs, 18 tRNAs and 9 gene fragments transferred from chloroplast were annotated. The RB867515 mitochondrial chromosomes showed differences when compared to those from S. officinarum, including single nucleotide polymorphisms (SNPs), genetic duplications and genomic expansions. / A cana-de-açúcar (Saccharum spp.) é uma das mais importantes culturas das regiões tropicais e subtropicais do mundo. A cana é cultivada em mais de 100 países, fornecendo matéria-prima para a obtenção de produtos como açúcar e bioetanol. Dada sua importância, diversos esforços vêm sendo realizados com o objetivo de se realizar a caracterização genômica de cultivares de cana-de-açúcar. Os genomas eucarióticos são distribuídos em diferentes compartimentos celulares que apresentam padrões distintos de herança. Plastídeose mitocôndrias possuem sistema genético próprio, contendo DNA, RNA e todos os componentes necessários para os processos de replicação, transcrição e síntese de proteínas, que ocorrem nestas organelas. Cloroplastos e mitocôndrias são organelas que têm como função principal a transdução de energia. Os cloroplastos são responsáveis pela conversão de energia luminosa em energia química, durante a fotossíntese. As mitocôndrias fornecem energia em forma de ATP, por meio da respiração celular. O presente trabalho foi desenvolvido com o objetivo de se realizar a montagem e a caracterização dos genomas cloroplastidial e mitocondrial da cultivar de cana-de-açúcar RB867515, utilizando dados de sequenciamento de nova geração Illumina e PacBio. Em cloroplastos, buscou-se identificar, pela utilização de reads longos obtidos pela tecnologia PacBio no processo de montagem, evidências de ocorrência de heteroplasmia cloroplastidial em cultivares modernas de cana-de- açúcar. No genoma mitocondrial investigou-se a ocorrência de variações genéticas e genômicas estruturais. Os assemblies foram obtidos pela utilização de reads organelares, selecionados através do mapeamento a sequências de referência de cloroplastos e mitocôndrias, disponíveis publicamente. Os assemblies obtidos foram realizados com os softwares SPAdes e Organelle_PBA. A anotação gênica foi realizada utilizando as ferramentas DOGMA, GeSeq e Mitofy. Foram identificados dois haplótipos (isoformas) de cloroplastos na cultivar RB867515. Estas isoformas diferem entre si pela ocorrência de orientações distintas da região SSC (small single copy), confirmando a hipótese de heteroplasmia cloroplastidial em cana-de-açúcar. Cada haplótipo é constituído por 141.181 pb e exibe uma estrutura quadripartida típica, que inclui uma região longa de cópia única (LSC) de 83.047 pb flanqueada por duas regiões de repetições invertidas (IRs) de 22.795 pb e uma pequena região de cópia única (SSC) entre as IRs de 12.544 pb. O genoma mitocondrial montado foi constituído por dois cromossomos: o cromossomo 1 de comprimento total de 300.765 pb e o cromossomo 2, de 194.383 pb. As estimativas obtidas para os conteúdos GC (~44%) e AT (~56%) foram concordantes com as de outras angiospermas. Foram anotados 39 CDSs, 5 genes hipotéticos conservados, 5 rRNAs, 18 tRNAs e 9 fragmentos de genes transferidos de cloroplastos. A comparação dos cromossomos mitocondriais da cultivar RB867515 com aqueles de S. officinarum permitiu a identificação de polimorfismos de bases únicas (SNPs), duplicações gênicas e expansões genômicas.

Les capacités d’auto-renouvellement ou de différenciation progressive en gamètes des cellules souches spermatogoniales (SSC) sont indispensables à la production de spermatozoïdes tout au long de la vie des individus de sexe mâle. Les travaux réalisés au cours de la thèse ont eu pour objectif de mieux comprendre les mécanismes cellulaires et moléculaires potentiellement impliqués dans le contrôle du devenir des SSC chez les poissons téléostéens. Grâce à une nouvelle lignée de poisson-zèbre transgénique exprimant la GFP sous le contrôle d’un nouveau fragment promoteur du gène vasa, nous avons caractérisé une sous-population de spermatogonies indifférenciées composés de doublets asymétriques. L’ensemble de nos observations suggère que ces doublets asymétriques constituent un pool de cellules souches recrutées pouvant soit poursuivre leur différenciation spermatique soit participer à la dynamique de renouvèlement du stock de SSC.Une approche de séquençage à haut débit des transcrits exprimés au sein des spermatogonies indifférenciées fluorescentes et des cellules de Sertoli met en évidence la diversité des voies de régulation paracrine potentiellement impliquées dans le contrôle du devenir des SSC (molécules d’adhésion cellulaire, cytokines, facteurs de croissance…). Les analyses phylogénétiques montrent que certaines de ces voies de régulation sont conservées chez les Gnathostomes alors que d’autres ont été perdues chez les tétrapodes ou les mammifères seulement. Une approche comparée initiée chez la truite arc-en-ciel confirme l’intérêt générique des données acquises chez / By their ability to self-renew or differentiate into gametes, the spermatogonial stem cells (SSCs) are essential for sperm production throughout the life of male. The work done during the thesis had for objective the understanding of the cellular and molecular mechanisms potentially involved in the fate of the SSC in the teleost fish. With a new line of transgenic zebrafish expressing GFP under the control of a new gene promoter fragment vasa, we characterized a subpopulation of undifferentiated spermatogonia composed of asymmetric doublet. The observation of the GFP protein indicates that these doublets can fragment to generate isolated spermatogonia. Transplantation of fluorescent spermatogonial cells into the abdominal cavity of a recipient embryo shows that these cells have all functional properties of SSC. All our observations suggest that asymmetric doublet constitute a pool of stem cells with bivalent fate:they can continue their sperm differentiation or participate in the dynamic renewal of SSC pool. A rnaseq based transcritpome analysis of transcripts expressed in undifferentiated spermatogonia fluorescent and Sertoli cells highlights the diversity of paracrine regulatory pathways potentially involved in the control of the SSC (cell adhesion molecules, cytokines, growth factors, primary metabolites). Phylogenetic analyzes show that some of these regulatory pathways are well conserved among all Gnathostomes while others were lost in tetrapods or mammals only. A comparative approach initiated in rainbow trout generic confirms the interest of the data acquired in th

This diploma thesis is focused on the area of outsourcing and Shared Services Centers. It includes an introduction to outsourcing, its history, the life cycle and the advantages and disadvantages associated with it. The paper is than focused on the Shared Services Centers model, their definition, description, history and benefits and risks arising from them. The thesis also analyzes problems of the implementation process of accounting shared services from a theoretical point of view. This theoretical part is followed by a case study about an establishment of SSC by Czech companies.

Diploma thesis deals with the topic of shared service center. Aim of this thesis is to charac-terize ICT SSC and propose a formalized procedure for the establishment of ICT SSC in the Czech Republic. To achieve its objective, the thesis is divided into two pats. The first part presents a theoretical framework of shared service centers. Newly acquired knowledge is used in the second part. The second part presents a formalized procedure establishing ICT shared service centers and case study. Case study is based on real project, which was implemented by an international retail group. The author's contribution to this work lies in comprehensive description of the problem and the integration of theory and practice.

The master’s thesis deals with two applications of gold discoidal nanostructures exhibiting plasmon resonance for biodetection. The first approach considers the detection of changes in the phase on plasmonic antennas using coherence-controlled holography microscope. It was found that the steepness of the phase is increasing with the illumination wavelength when plasmon resonance is excited in larger antennas. The sensitivity of the phase to refractive-index changes of the surrounding media was observed when the largest response was given by antennas in resonance with wavelength of illumination. Next part deals with plasmon resonance detection by means of optical spectroscopy combined with voltametry which characterizes the electrochemical activity. Changes in resonance wavelength induced by the presence of SSC buffer were observed, although this influence seems to diminish in time. Conducted experiments have also shown that oxygen-plasma cleaning is not suitable for sample surface cleaning because of oxidation of metals including gold as well.

No description available.

Yes / This article aims to assess critically different definitions and indicators of smart cities. Drawing on exemplary case studies, the author proposes a typology of four categories of smart cities: type A are the world leaders who pioneer ideas not predicated on smart city projects; type B are aspirational cities punching above their weight; type C are surprise transformers that use the smart city concept to propel real transformation; and type D are cases where smart city projects do not directly address the main urban problems. The discussion highlights the need to prevent ‘smart-wash’ by avoiding superficial technological solutions that chase symptoms but not causes of some of the complex urban challenges that they are intending to address. In conclusion, the author considers the public policy implications of applying these typologies to cities in general with particular reference to the Global South / British Academy: [grant number IPM 15008]

Purpose: to assess the retentive strengths of passive fit esthetic anterior restorations using three commercially available cements. Methods: Three resin dies were fabricated from the intaglio surface of each restoration type. Each die was prepared following the current accepted guidelines on primary anterior tooth crown preparation. The three prepared teeth were replicated to produce 30 dies for each of the three restoration types. The prepared teeth were further separated into nine groups of 10 teeth each. Thirty EZ Pedo Crowns, 30 NuSmile Primary Crowns and 30 Unitek crowns were cemented using hand pressure employing the luting cement assigned to the corresponding group. The units were allowed to cure for 7 days. The force required to dislodge the restoration was tested using the Instron Universal Testing Machine. The data was statistically analyzed using a two-way ANOVA to analyze the force required to dislodge the restorations. A two-way logistic regression was used to analyze the failure types. Results: There were no significant differences in restoration retention rates between restoration types (P = 0.4412) but there were significant differences between types of cements used. (P < .0001). The differences with regard to cement types were consistent across the restoration groups (P = 0.7682). Tukey’s HSD multiple comparison procedure indicated FujiCem was significantly more retentive than either Fuji I or Ketac Cem cements and there were no significant differences in restoration retention rates between the Fuji I and Ketac Cem cements. Conclusion: The type of restoration did not matter between cements but cement type did matter with FujiCem cement being more retentive than the other types of cements tested.

(二) 閉架調閱常見問題： 1. 調閱處理時限：自申請後二個工作日內供讀者取件，二個工作日包含回覆email時間。例如讀者於週一上午10點申請，至遲須於週三上午10點就可至櫃檯領取調閱書籍。由於時間短促，需確保調閱流程順暢無誤。 2. 借閱方式：調閱資料擬以越權方式辦理外借，並請讀者於當日閉館前三十分鐘歸還。校外讀者以臨時閱覽證辦理借閱，校內讀者以借書證辦理，但若校內讀者已借閱他館館藏、借閱冊數達上限時，可能造成讀者無法調閱資料。 3. 現場填單：未於線上申請調閱者，可於現場填寫申請單。 4. 急件判斷標準：以客為尊，讀者說了算，儘量滿足讀者要求。 5. 週六申請調閱問題：改行閉架之後，週六僅開放櫃檯取書，調閱申請案件將順延至週一處理。 6. 入口動線：施工期間讀者仍由一樓前棟大門進出，但門禁系統及入口櫃檯將拆除。需請廠商於施工時勿影響人員出入。