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Effects of Phytohormones on Scenedesmus quadricaudaAlam, Mohammad Ihtisham 08 1900 (has links)
The literature on the effects of phytohormone on algae is clouded with contradictory reports. Reports have been published which substantiate and deny the effects of phytohormones in enhancing the growth and developmental processes in algae. The overall aim of this study was to investigate the response, if any, of the phytohormones indole-3-acetic acid (IAA), gibberellic acid A3 (GA) and kinetin on the physiology of the green alga, Scenedesmus quadricauda. Results obtained for the uptake of 14^C-IAA an(j l4C-kinetin by Scenedesmus strongly support the presumption that the alga does not absorb the hormones. The retention of the phytohormones by the alga is due to adsorption, and is independent of hormone concentration. Most of the label was adsorbed by the outer pectic layers of the cell wall.
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Metabolism of Methylglyoxal by Scenedesmus QuadricaudaRounsavall, Terry Yale 12 1900 (has links)
This report describes the effects of methylglyoxal on several green and blue-green algae, its correlation with photosynthesis, and a possible explanation of its metabolic role in the algae.
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Akumulácia a toxicita selénu v riasach druhu Scenedesmus quadricauda / Bioaccumulation and toxicity of selenium compounds in the green alga Scenedesmus quadricaudaUMYSOVÁ, Dáša January 2011 (has links)
Three strains of the green alga Scenedesmus quadricauda were selected to be specifically resistant to the presence of high concentrations of inorganic Se. The strain SeIV was found to be resistant to high doses of selenite, the strain SeVI was resistant to selenate, and the strain Se(IV+VI) to the presence of both Se compounds. It has been shown that both the dose and chemical form of Se are the critical factors in the cellular response. In this PhD. thesis are results from experiments with the resistant strains (activity of thioredoxin reductase, content of selenium and selenomethionine, effect of sulphur deficiency).
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Metabolism of Methylglyoxal by Scenedesmus QuadricaudaRounsavall, Terry Yale 06 1900 (has links)
The purpose of this study was to investigate the metabolic pathways of methylglyoxal in S. quadricauda.
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Stanovení biologicky aktivních metabolitů jednobuněčných řas za různých kultivačních podmínekMalyugina, Svetlana January 2017 (has links)
This thesis studies effect of cultivation conditions on the production of secondary me-tabolites in the green microalgae Scenedesmus quadricauda. During the experiment was studied influence of pH, UVC radiation, light intensity and various temperature conditions on the amount of biologically active compounds (polyphenols, flavonoids, antioxidants). Metabolites were determined using biochemical analytical methods. The total content of polyphenols was determined using the Folin-Ciocalteu method, which is based on chemical reaction of phenols with Folin-Ciocalteu reagent. The total con-tent of flavonoids was determined spectrophotometrically using AlCl3.6H2O with the addition of NaNO2 and 1 M NaOH. The total antioxidant capacity (TAC) was deter-mined using phosphomolybdenun antioxidant assay. Antioxidant activity was stimu-lated by UVC radiation. The highest value being recorded when samples were exposed to UVC for 5 minutes. Light intensity and the temperature changes did not have significant effect on antioxidant activity of studied samples. There was an increase in TAC in samples cultured in acid pH medium (4 and 5). The content of polyphenolic and flavonoid compounds with high light intensity (3500, 4000 lx) and high tempera-tures (35 °C, 40 °C) decreased. Conversely, changes in pH and UV-C stimulate biosyn-thesis of both polyphenols and flavonoids. The greatest increase of these compounds was observed in the medium with pH = 4 and the time of exposure to UV-C for 5 minutes a day.
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Methylglyoxal Effects on Cell Division of Scenedesmus quadricauda (Scenedesmaceae)Rhie, Kitae 08 1900 (has links)
Cell division of ggeneflesmus quadricauda (Turp.) Breb. (Scenedesmaceae) is enhanced by methylglyoxal, a general inhibitor of cell division, at threshold concentration in conjunction with treatment timing related to growth stage of batch cultures. At 0.5 mM methylglyoxal concentration, cell division was significantly enhanced in algae treated in the logarithmic phase. Specific growth rates of methylglyoxal-treated cultures were rapidly increased at the beginning of logarithmic phase. Cultures inoculated with high cell numbers were less sensitive, but still showed high specific growth rates in logarithmic phase. Cell division in cultures which had low cell numbers was inhibited by 0.5 mM methylglyoxal treatment.
Both specific activity of Glyoxalase I and the ratio of Glyoxalase I to Glyoxalase II of methylgloxal-treated cultures were higher than those of controls (1.3 and 2.1- fold, respectively). Pyruvate concentration in treated cultures was increased after methylglyoxal treatment.
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Reakcia na poškodenie DNA v zelených riasach Chlamydomonas reinhardtii a Scenedesmus quadricauda / DNA damage response in green algae Chlamydomonas reinhardtii and Scenedesmus quadricaudaHLAVOVÁ, Monika January 2011 (has links)
The effect of FdUrd, zeocin, caffeine and their combination on the cell cycle of green algae Chlamydomonas reinhardtii and Scenedesmus quadricauda and response of these model organisms to DNA damage were studied. Both, FdUrd and zeocin, caused DNA damage that led to cell cycle arrest in these algae. In contrast, caffeine partially abolished G2 phase block imposed by zeocin. Protein levels of three crucial cell cycle regulators - CdkA, CdkB and Wee1 kinases were measured to identify mechanisms controlling reaction to DNA damage.
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Vliv inhibitorů BSO, Lycorine a AIP na biosyntézu sekundárních metabolitů jednobuněčné řasy Scenedesmus quadricaudaRankić, Ivan January 2018 (has links)
The theoretical overview summarize the characteristics of algae and their primary and secondary metabolism. It also deals with inhibitors of ascorbic acid, glutathione and polyphenolic compounds synthesis. The practical part studies the influence of selected inhibitors (BSO, Lyc, AIP) and CdCl2 on the secondary metabolites production and biomass growth in freshwater microalgae Scenedesmus quadricauda. The content of secondary metabolites (eg. caffeic acid, chlorogenic acid, salicylic acid, cinnamic acid, p-coumaric acid) was detected by HPLC-MS. Furthermore, the total antioxidant capacity, the total polyphenol content and the total flavonoid content was determined spectrophotometrically. All the inhibitors used, reduced S. quadricauda growth in comparison with control samples. The most pronounced inhibitory effect was observed in the samples treated with Lyc + Cd after 7 days of experiment. All the treated samples responded to the stress factors by altering metabolic pathways and inhibiting growth. As a result, there were changes in the composition and the amount of selected secondary metabolites versus the control samples. At the same time, in most cases, the biosynthesis of polyphenolic and flavonoid substances has been stimulated. The oxidative reduction equilibrium and the induction of oxidative stress were probably impaired.
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CULTIVO DA MICROALGA Scenedesmus quadricauda EM EFLUENTES DE BIODIGESTÃO DE AVES E SUÍNOSVendruscolo, Jose Benedito Girardello 21 September 2009 (has links)
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Previous issue date: 2009-09-21 / O alimento originado do plâncton que chega ao peixe é o zooplancton e sua preferência
alimentar na rede trófica são as microalgas, cuja autotrofia permite a riqueza de todos os
sistemas aquáticos do mundo. Na produção aquícola os estudos de organismos como as
microalgas, que possam permitir a produção sustentável na atividade, são necessários e
relevantes. A microalga Scenedesmus quadricauda tem características especiais tais como
digestibilidade, preferência de consumo, facilidade de cultivo. Objetivando a maximização da
produção autotrófica e redução de custos no cultivo da microalga Scenedesmus quadricauda,
dejetos biodigeridos de suínos e aves foram testados juntamente com meio de cultura Chu,
tradicionalmente utilizado para essa finalidade. Esses produtos são disponíveis e de baixo
custo na região do Sudoeste de Goiás, graças ao incremento regional da atividade de
suinocultura e avicultura e ao seu potencial de crescimento nos últimos anos. Assim, o
presente trabalho foi desenvolvido no Laboratório de Aquicultura do Departamento de
Zootecnia da Universidade Católica de Goiás, atendendo às condições ambientais necessárias
para cultivo da microalga Scnedesmus quadricauda em meio de cultivo tradicional. Durante
quatorze dias foram avaliadas as quatro repetições dos três tratamentos: meios de cultura Chu,
biodigerido de aves e biodigerido de suínos. Concluiu-se que o meio de cultivo Chu
tradicional pode ser substituído pelos meios de cultivo biodigerido de suínos e aves sem perda
na produção de células da microalga Scenedesmus quadricauda, uma vez que a comparação
entre eles não apresentou diferença significativa ao nível de 5% de probabilidade. Os picos de
crescimento que representam produtividade para os três meios de cultura diferiram entre si
com superioridade para biodigerido de aves e suínos em relação ao meio Chu.
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Fotosyntetická reakce autotrofních organismů v přítomnosti vybraného laktamu / Photosynthetic response of autotrophic organism in presence of selected lactamNOVÁK, Jan January 2014 (has links)
The aim of this work is to evaluate the effect of caprolactam on the process of photosynthesis in model representatives of green algae (Scenedesmus quadricauda) through the dynamics of the release of oxygen into the solution of medium.
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