I will analyze the transcript from the 2000 BPAC meeting on the reevaluation of the MSM deferral policy and will elucidate the role that scientific data, specifically the data associated with NAT, plays in the 2000 deliberations surrounding the MSM deferral. This examination reveals that while scientific data did play a significant role in the decision making process of the BPAC there were also a number of other factors that influenced their deliberations as well. Ultimately what I will argue is that there were two different platforms present in the meeting and that each platform performs and enacts the body and blood of the donor differently. One platform is all about the inclusionary principles of blood donation and is most concerned by the potential for discrimination toward the body of the donor. The other platform is all about risk regulation and economics and is most concerned about how the body and blood of the donor will affect the safety and integrity of the blood supply. These platforms are not perspectival or dependent on view; this is not an epistemological argument but rather, an ontological one that concerns the reality and materiality of the situation, not the perspective. Thinking about these two platforms gives a handle to the nature of this controversy and contextualizes the committee’s decision to continue with the MSM deferral.

The aim of this study is to investigate the views of preservice science teachers on science-technology-society, STS, issue. A total of 176 preservice science teachers participated in the study. A 26-item &ldquo / Views on Science-Technology-Society (VOSTS)&rdquo / instrument, translated and adapted into Turkish, were utilized to assess participants&rsquo / views on STS. The VOSTS (Aikenhead, Ryan and Fleming, 1989) is a pool of 114 empirically developed multiple-choice items with nine categories. In order to understand participants&rsquo / views on STS in depth, semi-structured interviews were also conducted by 9 volunteer preservice science teachers. The results gave a colorful picture of the views of preservice science teachers on science-technology-society issue. The analysis revealed that preservice science teachers often confuse the definitions of technology with science. Most of the participants of the study had specific views about the reasons of doing scientific researches in their country, for example, to be independent from other countries, to get financial profit. Results displayed a consensus on the possible positive effects of upbringing and the importance of education given to high school students. According to the data obtained from the present study, respondents possess varied views about the influences of society on science and technology. While preservice science teachers claiming that scientists could break the rules of science, they also claimed scientists as objective in their study. On the other hand, participants supported the view that scientists&rsquo / concern on all the effects of their experiments. Preservice science teachers advocated also that technological developments can be controlled by citizens.

Diss. Universität Bremen, 2008. / Business and Economics (German Language) (Springer-11775) (GWV).

Diss. Universität Bremen, 2008. / Business and Economics (German Language) (Springer-11775) (GWV).

Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (leaves 109-124).

Thesis (Ph. D.)--History, Technology and Society, Georgia Institute of Technology, 2007. / Rosser, Sue V., Committee Member ; Alexander, Eleanor, Committee Member ; Bayor, Ronald, Committee Member ; Hammonds, Evelynn M., Committee Member ; Usselman, Steven W., Committee Co-Chair ; Pearson, Willie Jr., Committee Co-Chair.

Enterohemorrhagic E. coli, EHEC, is a verotoxin producing, zoonotic pathogen, which causes diseases in humans such as bloody or watery diarrhea. Microorganisms compete for limited living space, nutrients and other resources and therefore other microorganisms are EHECs biggest competitors. To avoid outbreaks and infections with EHEC, one possible approach is to use harmless but competitive bacteria as probiotics. Therefore, the aim of this study was to evaluate three probiotic E. coli strains and their ability to outcompete EHEC in bovine feces.     Ten different cattle fecal samples from three different farms were used to mix with the three probiotic and EHEC strains. The mixture was diluted and cultivated at 0 h as a control and then incubated for 48 h at 20°C and 37°C before dilution and cultivation on CT-SMaC. Colonies was counted and ratios between EHEC and probiotic E. coli before and after incubation were calculated. Kruskal-Wallis test with Dunn’s test as post hoc test were used to see if observed reductions of EHEC were significant or not.     In 37°C, strain 10 was the only strain producing a significant reduction of EHEC. In contrast, no significant reduction was observed at 20°C in any of the strains.     Future research studying other factors and performed on live cattle models are necessary to confirm the usefulness of the studied probiotic candidates. However, these results indicate probiotics can be a useful tool to avoid infections and big outbreaks of EHEC in the future.

Type 1 diabetes(T1D)is a disease that only gets more common. The etiology of the disease is not known but there are many existing theories about what the cause is. These different theories have been tested in vivoin rodents or invitro. The resultsfrom experiments done in those waysarenotall realistic because rodents differnotablyfrom humans,and when studies areperformed in vitrowith human isletsof Langerhans different hormones will accumulate. The aim of this studywas to establisha dynamic ex vivosystem in which stimulation of human islets of Langerhans can be performed in a more lifelike environment. To study islets in this system couldin the future lead to increased knowledge in the etiology of T1D.The perifusion system PERI-4.2 from Biorep Technologies together with an incubator with 37°Cand5% CO2were used to arrangethe ex vivosystem. An Insulin ELISA from Mercodia was performedto analyze the insulin secretion from the islets. Fourdifferent set ups for the system were tested and the last one showed the best results.In conclusion this study has shown that it is possible to preserve human islets of Langerhans in a dynamic ex vivosystem with a constant medium exchange if it is done under conditionswhere the islets are protected from shear forces from the supplying medium,together with a medium exchange rate which replaces the whole medium in at least one hour.

Background: Insulin is an important hormone for glucose homeostasis. It is released from β-cells in the endocrine pancreas as a response to increased concentrations of plasma glucose. The major effect of insulin is the facilitation of cellular uptake and storage of glucose as glycogen. Insulinomas are tumours that produce excessive amounts of insulin resulting in hypoglycaemia. The condition has been observed in dogs and cats and is often malignant. One part of establishing the diagnosis is confirmation of elevated concentrations of insulin in a hypoglycaemic sample. Aim: The aim of this study was to evaluate if an equine-optimized insulin ELISA (Mercodia AB, Uppsala, Sweden) is useful for analysis of insulin in canine and feline serum samples when insulinoma is suspected. Material and methods: All samples were analysed with Equine insulin ELISA. Precision, linearity and effects of haemolysis were studied. The stability of insulin was evaluated after storage in 4°C, room temperature and after repeated freezing and thawing. A reference interval was constructed for both canine and feline samples. Results: Total precision expressed as CV was 4.4 – 18.9 %. The method was linear up to at least 100 mU/L for dogs and 15 mU/L for cats. Reference interval for cats was <11.6 mU/L, due to few healthy animals no reference interval for dogs could be established. Stability was acceptable for up to four days. No effects of haemolysis were detected. Conclusion: Mercodia Equine insulin ELISA is suitable for analysis of insulin in serum from dogs and cats when suspecting insulinoma.

1ABSTRACTCarbohydrate-deficient transferrin (CDT) is a biomarker for excessive and long-running intake of alcohol. It is a form of transferrin called disialotransferrin that under normal circumstances is <2 % of total transferrin in human blood. An increase is seen when alcohol consumption exceeds450 g per week. CDT is analyzed in serum usinghigh performance liquid chromatography (HPLC) and UV/VIS detection. The purpose of this study was to investigate if an “in routine” method could be improved by switching columns.With ion exchange chromatography transferrin glycoforms are separated and quantified. The carbohydrate-deficient transferrin glycoforms have an isoelectric point between 5,7-5,9 that depends on the number of sialic acids on the molecule. With the use of a salt gradient and pH above the isoelectric point the glycoforms can beseparated depending on their affinity to the stationary phase. Batches with patient and control serum was first analyzed on the routine column Source 15Q PE and then on the alternative column Reprospher 200 SAX 5μm.Student’s t-test showed that the two methods’results correlated but were significantly different. A Bland-Altman plot illustrated differences between the two columns. High and low control serum values from Reprospher were lower than the accepted interval. In this study Reprospher’s stationary phase seemed to be affected to such an extent that stabile retention time, better resolution, and stabile values could not be achieved and because the information about the column was lacking an attempt to regeneratethe columnwas not conducted.