Three different groups of materials were chosen to investigate the uniaxial compaction of particulate solids. Dentritic and cubic sodium chloride were chosen as plastically deforming, dicalcium phosphcte and sugar as fragmentary and styrocell, homopolymer and copolyrinier as non-compactable materials. The uniaxial compaction of the materials was continuously followed by measurement of the applied force, the force transmitted radially to the die wall and the upper punch displacement. The data obtained was presented in the form of Mohr circles, stress pathways (shear-mean compaction stress planes) and a three dimensional representation in mean compaction stress, shear stress and volume change. The yield loci evaluated from Mohr circles and shear-mean compaction stress relationships of compactable and non-compactable materials were found to be similar in shape. The unloading stress profiles were however more informative. All unloading shear-mean compaction stress curves of the compactable materials cross the mean compaction stress axis to give negative values of shear stress and reach a minimum value of ^t_min' which was material and compaction pressure dependent. The unloading curves of non-compactable materials gave approximately zero shear. The parameters evaluated from the characteristic stress profiles were correlated to the tensile strength and hardness of compacts. Mathematical expressions have been proposed for the shear-mean compaction stress relationships of the materials investigated. The materials were characterised before and after compaction in terms of specific surface area, porosity and mechanical strength of compacts with compaction pressure.

No description available.

Jet in crossflow (JICF) configurations are often used for secondary fuel injection in staged-fuel combustion systems. The high temperature, vitiated crossflow in these systems is inherently unsteady and characterized by random, turbulent fluctuations and coherent, acoustic oscillations. This thesis presents the results of an experimental investigation into the dynamics of non-reacting and reacting jets injected into unsteady, vitiated crossflow. The flow structure and flame stabilization of jets with different momentum flux and density ratios relative to the crossflow are characterized using simultaneous time-resolved stereoscopic particle image velocimetry (SPIV) synchronized with OH planar laser induced fluorescence (PLIF). A modified trajectory scaling law is developed to account for the influence of near-field heat release on the jet trajectory. The second part of this work focuses on the response of a JICF to crossflow forcing. Acoustic drivers are used to excite natural resonances of the facility, which are characterized using the two-microphone method. Spectral analysis of SPIV results shows that, while the jet response to crossflow velocity fluctuations is often negligible, the fluctuating crossflow pressure induces a significant fluctuating jet exit velocity, which leads to periodic jet flapping. The flame response to crossflow forcing is studied using flame edge tracking. An analytical model is developed that predicts the dependence of the jet injector impedance upon important JICF parameters. In the final part of this work, vortex tracking and Mie scattering flow visualization are used to investigate the effect of near-field heat release on the shear layer dynamics. A phenomenological model is developed to explain the effect of combustion on the shear layer stability of density stratified, reacting JICF. The results of this study demonstrate the important effects of near-field heat release and crossflow acoustics on the dynamics of reacting JICF.

The subject of the thesis is concerned with the study of the turbulent spreading and the gravity spreading processes in surface jets and surface plumes influenced by the cross-flow. A total of five different discharge and cross-flow configurations were under investigation. They are, namely, (i) submerged jets in unconfined cross-flow, (ii) surface jets in deep and shallow cross-flows, (iii) surface plumes in deep cross-flow, (iv) full-depth jets in cross-flow and (v) full-depth plumes in cross-flow, reported in Chapters IV, V, VI, VII and VIII respectively. / The effect of the gravity stratification, the effect of the free water surface and the confined effect of the channel bottom on the spreading of the turbulent flows have been determined from control experiments. The experimental data have been correlated in a unified manner, using suitable scales and dimensionless parameters. They are also related to a general integral formulation, established for this type of problems. / The results of an extensive series of previous experimental investigations, of related problems of jets and plumes in cross-flow have been reanalysed and they are integrated into the various parts of the thesis to complement and confirm the present investigation. / On the whole, the thesis has succeeded in providing a general description of the turbulent spreading and the gravity spreading processes in flows of different discharge and cross-flow configurations.

No description available.

Shear and extensional flows of dilute polymer solutions were studied experimentally in an attempt to understand the mechanism of polymer-induced drag reduction. A flowcell capable of simulating the dynamics of a turbulent boundary layer, involving the motion of counter-rotating vortices, was designed and fabricated. The pressure drop across the flowcell was measured for different flow arrangements, first with a Newtonian fluid and then with drag reducing, dilute polymer solutions. The pressure drop in excess of the Newtonian baseline, after accounting for viscous effects, was used as a measure of elastic effects. With the dilute polymer solutions, elastic effects were observed both in shear, extensional, as well as presheared extensional flows. These effects can be attributed to additional normal stresses generated by shearing. For extensional flows, the observed effects were independent of elongation rates, indicating that a conclusion regarding the mechanism of drag reduction cannot be made from the flowfield investigated.

Endothelial cells (ECs) respond to flow but the exact mechanism producing alignment is not completely understood. We characterized EC alignment in microfluidic channels, 4 mm wide by 350 um high, to generate shear of 20 dynes / cm2 across the cell surface. In microchannels, ECs aligned perpendicular under flow. Analytical tools were developed to quantify nuclear alignment at 67% for human umbilical vein endothelial cells (HUVECs); cell elongation under shear flow shifted aspect ratio from 2.41 to 2.86. We next sought to probe the mechanism through which ECs communicate during realignment. The planar cell polarity (PCP) signaling pathway is involved in cell organization and coordination during development. A number of genes are known to affect the formation and organization of cellular structures through PCP signaling in human ECs. Higher expression of Vangl1 and Dvl1 proteins did not alter cell reorganization; knockdown of Vangl1 expression decreased EC alignment.

Experimental research was conducted to investigate the influence of axial load and prestress on the shear strength of web-shear critical reinforced concrete elements. The ability of two design codes, the ACI code and the CSA code, to accurately predict the shear strength of web-shear critical reinforced concrete elements was investigated through two sets of experiments performed for this thesis, the panel tests and the beam tests. The experimental results indicated that the CSA code provided better predictions for the shear strength of web-shear critical reinforced concrete members subjected to combined axial force and shear force than the ACI code. A total of six panels, reinforced almost identically, were tested under different combinations of uni-axial stress and shear stress. In addition to the panel tests, a total of eleven I-shaped beams, with the same web thickness, were tested under different combinations of axial force and shear force. The parameters for these beams were the amount of longitudinal reinforcement, the amount of transverse reinforcement, and the thickness of the flanges. The beams were simply supported, but the loading geometry was specially designed to simulate the loading conditions in continuous beams near points of inflection. The experimental results from the panel tests and the beam tests followed a similar trend of variations. Both the inclined cracking strength and the ultimate shear strength were increased by compression and were reduced by tension. The specimens subjected to very high compression failed explosively without developing many cracks. The inclined cracking strength could be predicted with good accuracy if the influence of the co-existing compression on the cracking strength of the concrete and the non-uniform distribution of the stresses over the depth of the cross-section were considered. The strength predictions using the ACI code for these tests were neither accurate nor consistent. The ACI code was unconservative for members subjected to compression and was excessively conservative for members subjected to tension. In contrast, the strength predictions using the CSA code for these tests were generally conservative and consistent. The CSA code accurately predicted the response of specimens subjected to compression and was somewhat conservative in predicting the shear strength of specimens subjected to tension.

I present physical shear-box experiments investigating the relationship between geometrical properties of shear zones and mechanical properties of deformed rocks. Experimental methodology is also examined critically and new materials for analogue modelling of shear localization are presented. First, I tested experimentally whether meaningful rheological information can be deduced from finite geometrical shear zone data. The results predict characteristic geometrical responses for certain end-member materials. However, it will be difficult to constrain such responses in the field. In the second part physical controls on deformation in the shear box are analysed for Newtonian and power-law fluids and an elastoviscoplastic strain-softening material. Since models always represent simplifications of the natural problem, it is essential to understand fully the physics of a given simulation. I show that displacement boundary conditions, model geometry, and rheology control shear zone geometry. Practical applications of the shear box for modelling natural shear localization and limitations of isothermal physical models with displacement boundary conditions in general are discussed. In the third part, new data on the rheology of highly-filled silicone polymers are introduced. Since dynamic similarity must be satisfied in analogue models to permit scaled, quantitative simulations of deformation processes, the choice of suitable rock analogues is critical for physical experiments. In particular, we address the problem of designing power-law fluids to model rocks deforming by dislocation creep. We found that highly-filled polymers have complex rheologies. Hence, such materials must be used with care in analogue modelling and only for certain experimental stress-strain rate conditions. Finally, I investigated whether fault network geometry and topography of brittle strike-slip faults are influenced by the degree of compaction of the host rock. Analogue shear experiments with loose and dense sand imply that the degree of sediment compaction may be a governing factor in the evolution of fault network structure and topography along strike-slip faults in sedimentary basins. Therefore, models of strike-slip faults should consider potential volume changes of deformed rocks.

During inflammation, leukocytes modulate α4β1(VLA-4) integrin avidity in order to rapidly stabilize nascent adhesive contacts to VCAM-1-expressing endothelial cells and resist detachment forces imparted by the flowing blood. Linkage to the actin cytoskeleton is critical for integrin function, yet the exact role of the actin cytoskeleton in leukocyte adhesion stabilization under conditions of fluid flow remains poorly understood. We modeled leukocyte (U937 cell, mouse lymphocyte and human monocyte) arrest and adhesion stabilization through the use of a parallel plate flow chamber and visualized cells by phase contrast or fluorescent confocal microscopy. Live cell imaging with Lifeact-transfected U937 cells revealed that mechanical forces imparted by fluid flow induced formation of upstream tension-bearing anchors attached to the VCAM-1-coated surface. Scanning electron microscopy confirmed that flow-induced mechanical force culminates in the formation of structures that anchor monocyte adhesion. These structures are critical for adhesion stabilization, since disruption of actin polymerization dramatically inhibited VLA-4-dependent resistance to detachment, but did not affect VLA-4 expression, affinity modulation, and clustering or constitutive linkage to F-actin. Transfection of dominant-negative constructs and inhibition of kinase function or expression revealed key signaling steps required for upstream actin polymerization and adhesion stabilization. Rap1 was shown to be critical for resistance to flow-induced detachment and accumulated in its GTP form at the sites of anchor formation. A key mediator of force-induced Rac activation and actin polymerization is PI3K. Live cell imaging revealed accumulation of PIP3 within tension-bearing anchors and blockade of PI3K or deficiency of PI3Kγ isoform reproduced the adhesion defect produced by inhibition of actin polymerization. Thus, rapid signaling and structural adaptations enable leukocytes to stabilize adhesion and resist detachment forces; these included activation of Rap1, phosphoinositide 3-kinase γ-isoform and Rac, but not Cdc42.