The process of shock-induced ignition has been investigated both computa- tionally and experimentally, with particular emphasis on the concept of radical farming. The first component of the investigation contained Computational Fluid Dynamic (CFD) calculations of an ignition delay study, a 2D pre-mixed flow over flat plate at a constant angle to the freestream, and through a generic 2D scramjet model. The focal point of the investigation however examined the complex 3D flow through a generic scramjet model. Five experimental test conditions were ex- amined over flow enthalpies from 3.4 MJ/kg to 6.4 MJ/kg. All test conditions simulated flight at 21000 metres ([symbol=almost equal to] 70000 ft), while the equivalent flight Mach number varied from approximately 8.5 at the lowest enthalpy, to approximately Mach 12 at the highest enthalpy condition. The presence of H2 fuel injected in the intake caused a separated region to form on the lower surface of the model at the entrance to the combustor. A fraction of the total mass of fuel was entrained in this separated region, providing long residence times, hence increased time for the chemical reactions that lead to ignition to occur. In addition, extremely high temperatures were found to exist between each fuel jet. Both fuel and air are present in these regions, therefore the chance of ignition in these regions is high. Streamlines passing through the recirculation zone ignited within this zone, while streamlines passing between the fuel jets ignited soon after entry into the combustor. The first instance of a pressure rise from combustion was observed on the centreline of the model where the reflected bow shock around the fuel jets crossed the centreline of the combus- tor. Upstream of this location the static pressure of the flow was too low for the chemical reactions that release heat to occur. The comparison between the experimental and computational results was lim- ited due to inaccuracies in modelling the thermal state of the gas in the CFD calculations. The gas was modelled as being in a state of thermal equilibrium at all times, which incorrectly models the freestream flow from the nozzle of the shock tunnel, and also the flow downstream of oblique shock wave within the scramjet model. As a result combustion occurs sooner in the CFD calculations than in the experimental result.

The urokinase receptor (uPAR) plays a central role in metastatic process. It???s evident uPAR is overexpressed across a variety of tumour cells and leads to the increased aggressiveness and poor prognosis of cancer. Inhibition of uPAR expression can block metastatic potential in many tumours. In addition, besides uPA, there are several other proteins which have been confirmed to interact with uPAR, such as vitronectin and integrins. These interactions also contribute to signal transduction and the functions of uPAR complex. Therefore, downregulation of uPAR expression by targeting uPAR mRNA or protein, or by regulating the uPAR partners would be potential therapeutic strategies for prevention of cancer metastasis. There are two main aspects contained in this thesis. Firstly, three specific DNAzymes targeting uPAR mRNA were designed to downregulate uPAR expression in vitro and their effects to decrease cancer cell invasion studied in a human osteosarcoma cell line Saos-2. The results showed that two of them (Dz483 and Dz720) cleaved uPAR transcript in vitro with high efficacy and specificity and the Dz720 inhibited uPAR protein levels by 55% in Saos-2 cells. Besides, the Dz720 significantly suppressed Saos-2 cell invasion using an in vitro matrigel assay. Secondly, two potential uPAR partners from yeast two-hybrid screening, a heat shock protein MRJ and an anti-apoptosis protein HAX-1, were characterised and their functions binding with uPAR investigated. The interactions were confirmed by co-immunoprecipitation, GST-pull down assay and confocal microscopy in cancer cells. In addition, there was a 50% increase in cell adhesion after transfection with MRJ. This increase in adhesion is dependent on the uPAR/full length MRJ interaction as cells transfected with the mutant construct containing only N-terminal region or C-terminal region of MRJ had no increase in cell adhesion. The observed increase in adhesion to vitronectin by MRJ was also blocked by an anti-uPAR domain I antibody suggesting that the induced adhesion is at least in part contributed by uPAR on the cell surface. Together, the identification of both MRJ and HAX-1 as uPAR interactors provides further insight into the intricate relationship between uPAR and other proteins which may develop potential approaches for cancer therapy.

This thesis is concerned with migrants’ experience of their acceptance and well-being in Australian society, particularly the unconscious processes reflected in dreams and communication patterns; the provision of services intended to be of help in settlement; and the relationship between the unconscious processes and the provision of services. Collaborating with clients, colleagues who share similar interests and concerns, people with special skills and cultural knowledge, and some Management Committee members of the Migrant Resource Centre of Canberra and Queanbeyan, Inc. the author has investigated the multicultural unconscious, government policies and guidelines related to services to recent arrivals and people of non-English-speaking backgrounds, measures to address gaps in services for appropriate improvement. The research approach is naturalistic with a strong emphasis on the author’s personal reflections and case studies of people and projects. / Master of Science (Hons) Social Ecology

A comprehensive understanding of high-speed liquid jets is required for their introduction into engine and combustion applications. Their transient nature, short lifetime, unique characteristics and the inability to take many experimental readings, has inhibited this need. This study investigates the outflow of a high-speed liquid jet into quiescent atmospheric air. The key characteristics present are, a bow shock wave preceding the jet head, an enhanced mixing layer and the transient deformation of the liquid jet core. The outflow regime is studied in an experimental and numerical manner. In the experimental investigation, a high-speed liquid water jet is generated using the momentum exchange by impact method. The jet velocity is supersonic with respect to the impinged gaseous medium. The resulting jet speed is Mach 1.8. The jet is visualised with the use of shadowgraph apparatus. Visualisation takes place over a variety of time steps in the liquid jet???s life span and illustrates the four major development stages. The stages progress from initial rapid core jet expansion to jet stabilisation and characteristic uniform gradient formation. The visualisation shows that at all stages of the jet???s life it is axi-symmetric. One dimensional nozzle analysis and a clean bow shock wave indicate that the pulsing jet phenomenon can be ignored. In the numerical investigation, a time marching finite volume scheme is employed. The bow shock wave characteristics are studied with the use of a blunt body analogy. The jet at a specific time frame is considered a solid body. The jet shape is found to have an important influence on the shock position and shape. Analysis of the results indicates a shock stand-off similar to that seen in experimental observations and the prediction of shock data. The jet life span is modelled using a species dependent density model. The transient calculations reproduce the key jet shape characteristics shown in experimental visualisation. The mushrooming effect and large mixing layer are shown to develop. These effects are strongest when the shock wave transience has yet to stabilise. Quantitative analysis of the mixing layer at varying time steps is presented.

Currently shock effects on surface ships can be determined by full scale shock trials, Finite Element Analysis or semi empirical methods that reduce the analytical problem to a limited number of degrees of freedom and include hull configurations, construction methods and materials in an empirical way to determine any debilitating effects that an explosion may have on the ship. This research has been undertaken to better understand the effect of hull shape on surface ships' shock response to external underwater explosions (UNDEX). The study is within the semi empirical method category of computations. A set of simple closed-form equations has been developed that accurately predicts the magnitude of dynamic excitation of different 2- D rigid-hull shapes subject to far-field UNDEX events. This research was primarily focused on the affects of 2-D rigid hull shapes and their contribution to global ship motions. A section of the thesis,

Thesis (Ph. D.)--Ohio State University, 2007. / Title from first page of PDF file. Includes bibliographical references (p. 199-227).

This study investigates the stress wave force balance technique for the measurement of forces on a fuelled hypersonic flight vehicle in an impulse-type test facility. A three component force balance for the measurement of lift, thrust and pitching moment on a supersonic combustion ramjet engine was designed, built, calibrated and tested. The force balance was designed using finite element analysis and consisted of four stress bars instrumented for the measurement of strain. Relative errors of less than 2% were obtained for the recovered simulated calibration loads, while errors of less than 3% were obtained for lift and thrust components for simulated fuel-on and fuel-off force loading distributions. Tests in a calibration rig showed that the balance was capable of recovering the magnitude of point loads to within 3% and their lines of action to within 1% of the chord of the model. Additional errors result when testing in a wind tunnel. The uncertainties for the experiments with fuel injection are estimated at 9%, 7% and 9% for the coefficients of lift, thrust and pitching moment. The scramjet vehicle was 0.566m long and weighed approximately 6kg. It consisted of an inlet, combustion chamber and thrust surface. Fuel could be injected through a series of injectors located on the scramjet inlet. The scramjet model was set at zero angle of attack. Experiments were performed in the T4 Free Piston Shock Tunnel at a total enthalpy of 3.3MJ/kg, a nozzle supply pressure of 32MPa and a Mach number of 6.6, with equivalence ratios up to 1.4. Fuel-off force coefficients were measured to within 2% of theoretical values based on predictions using CFD and hypersonic theory. The fuel-off centre-of-pressure was measured to within 4% of the predicted value. The force coefficients varied linearly with equivalence ratio. Good comparison of the measured lift and thrust forces with theoretical values was obtained with increasing flow rates of fuel. The lift-to-drag ratio increased from 3.0 at the fuel-off condition to 17.2 at an equivalence ratio of 1.0. Poor agreement between the measured pitching moment and theoretical values was obtained due to difficulties in predicting the pressure distribution with heat addition on the latter parts of the thrust surface. A shift in the centre-of-pressure of approximately 10% of model chord was measured as the equivalence ratio varied from 0.0 to 1.0. For the design tested, the thrust produced was not enough to overcome drag on the vehicle, even at the highest equivalence ratio tested. Tests at higher stagnation enthalpies (up to 4.9MJ/kg) showed the lift and pitching moment coefficients remained constant with an equivalence ratio of 0.8 but the thrust coefficient decreased exponentially with increasing stagnation enthalpies. Good agreement of experimental values of lift and thrust force with predicted values was obtained for equivalence ratios of 0.0 and 0.8. Choking occurred at stagnation enthalpies of less than 3.0MJ/kg and a nozzle supply pressure of 32MPa with fuel injection at an equivalence ratio of approximately 0.8, resulting in a drag force of approximately 2.5 times the fuel-off drag force. Tests at a nozzle supply enthalpy of 3.3MJ/kg and nozzle supply pressures of 32, 26 and 16MPa were performed at equivalence ratios of 0.0 and 0.8. The fuel-off lift coefficient remained constant but the thrust coefficient increased. This is attributed to a reduction in skin friction associated with longer lengths of laminar boundary layers as the Reynolds number was decreased. The measured fuel-off lift and thrust coefficients agreed with the predicted values to within the known test flow and force prediction uncertainties. Combustion did not occur at a nozzle supply pressure of 16MPa. This work has demonstrated that overall scramjet vehicle performance measurements (such as lift-to-drag ratio and shifts in centre-of-pressure) can be made in a free piston shock tunnel.

This study investigates the stress wave force balance technique for the measurement of forces on a fuelled hypersonic flight vehicle in an impulse-type test facility. A three component force balance for the measurement of lift, thrust and pitching moment on a supersonic combustion ramjet engine was designed, built, calibrated and tested. The force balance was designed using finite element analysis and consisted of four stress bars instrumented for the measurement of strain. Relative errors of less than 2% were obtained for the recovered simulated calibration loads, while errors of less than 3% were obtained for lift and thrust components for simulated fuel-on and fuel-off force loading distributions. Tests in a calibration rig showed that the balance was capable of recovering the magnitude of point loads to within 3% and their lines of action to within 1% of the chord of the model. Additional errors result when testing in a wind tunnel. The uncertainties for the experiments with fuel injection are estimated at 9%, 7% and 9% for the coefficients of lift, thrust and pitching moment. The scramjet vehicle was 0.566m long and weighed approximately 6kg. It consisted of an inlet, combustion chamber and thrust surface. Fuel could be injected through a series of injectors located on the scramjet inlet. The scramjet model was set at zero angle of attack. Experiments were performed in the T4 Free Piston Shock Tunnel at a total enthalpy of 3.3MJ/kg, a nozzle supply pressure of 32MPa and a Mach number of 6.6, with equivalence ratios up to 1.4. Fuel-off force coefficients were measured to within 2% of theoretical values based on predictions using CFD and hypersonic theory. The fuel-off centre-of-pressure was measured to within 4% of the predicted value. The force coefficients varied linearly with equivalence ratio. Good comparison of the measured lift and thrust forces with theoretical values was obtained with increasing flow rates of fuel. The lift-to-drag ratio increased from 3.0 at the fuel-off condition to 17.2 at an equivalence ratio of 1.0. Poor agreement between the measured pitching moment and theoretical values was obtained due to difficulties in predicting the pressure distribution with heat addition on the latter parts of the thrust surface. A shift in the centre-of-pressure of approximately 10% of model chord was measured as the equivalence ratio varied from 0.0 to 1.0. For the design tested, the thrust produced was not enough to overcome drag on the vehicle, even at the highest equivalence ratio tested. Tests at higher stagnation enthalpies (up to 4.9MJ/kg) showed the lift and pitching moment coefficients remained constant with an equivalence ratio of 0.8 but the thrust coefficient decreased exponentially with increasing stagnation enthalpies. Good agreement of experimental values of lift and thrust force with predicted values was obtained for equivalence ratios of 0.0 and 0.8. Choking occurred at stagnation enthalpies of less than 3.0MJ/kg and a nozzle supply pressure of 32MPa with fuel injection at an equivalence ratio of approximately 0.8, resulting in a drag force of approximately 2.5 times the fuel-off drag force. Tests at a nozzle supply enthalpy of 3.3MJ/kg and nozzle supply pressures of 32, 26 and 16MPa were performed at equivalence ratios of 0.0 and 0.8. The fuel-off lift coefficient remained constant but the thrust coefficient increased. This is attributed to a reduction in skin friction associated with longer lengths of laminar boundary layers as the Reynolds number was decreased. The measured fuel-off lift and thrust coefficients agreed with the predicted values to within the known test flow and force prediction uncertainties. Combustion did not occur at a nozzle supply pressure of 16MPa. This work has demonstrated that overall scramjet vehicle performance measurements (such as lift-to-drag ratio and shifts in centre-of-pressure) can be made in a free piston shock tunnel.

This study investigates the stress wave force balance technique for the measurement of forces on a fuelled hypersonic flight vehicle in an impulse-type test facility. A three component force balance for the measurement of lift, thrust and pitching moment on a supersonic combustion ramjet engine was designed, built, calibrated and tested. The force balance was designed using finite element analysis and consisted of four stress bars instrumented for the measurement of strain. Relative errors of less than 2% were obtained for the recovered simulated calibration loads, while errors of less than 3% were obtained for lift and thrust components for simulated fuel-on and fuel-off force loading distributions. Tests in a calibration rig showed that the balance was capable of recovering the magnitude of point loads to within 3% and their lines of action to within 1% of the chord of the model. Additional errors result when testing in a wind tunnel. The uncertainties for the experiments with fuel injection are estimated at 9%, 7% and 9% for the coefficients of lift, thrust and pitching moment. The scramjet vehicle was 0.566m long and weighed approximately 6kg. It consisted of an inlet, combustion chamber and thrust surface. Fuel could be injected through a series of injectors located on the scramjet inlet. The scramjet model was set at zero angle of attack. Experiments were performed in the T4 Free Piston Shock Tunnel at a total enthalpy of 3.3MJ/kg, a nozzle supply pressure of 32MPa and a Mach number of 6.6, with equivalence ratios up to 1.4. Fuel-off force coefficients were measured to within 2% of theoretical values based on predictions using CFD and hypersonic theory. The fuel-off centre-of-pressure was measured to within 4% of the predicted value. The force coefficients varied linearly with equivalence ratio. Good comparison of the measured lift and thrust forces with theoretical values was obtained with increasing flow rates of fuel. The lift-to-drag ratio increased from 3.0 at the fuel-off condition to 17.2 at an equivalence ratio of 1.0. Poor agreement between the measured pitching moment and theoretical values was obtained due to difficulties in predicting the pressure distribution with heat addition on the latter parts of the thrust surface. A shift in the centre-of-pressure of approximately 10% of model chord was measured as the equivalence ratio varied from 0.0 to 1.0. For the design tested, the thrust produced was not enough to overcome drag on the vehicle, even at the highest equivalence ratio tested. Tests at higher stagnation enthalpies (up to 4.9MJ/kg) showed the lift and pitching moment coefficients remained constant with an equivalence ratio of 0.8 but the thrust coefficient decreased exponentially with increasing stagnation enthalpies. Good agreement of experimental values of lift and thrust force with predicted values was obtained for equivalence ratios of 0.0 and 0.8. Choking occurred at stagnation enthalpies of less than 3.0MJ/kg and a nozzle supply pressure of 32MPa with fuel injection at an equivalence ratio of approximately 0.8, resulting in a drag force of approximately 2.5 times the fuel-off drag force. Tests at a nozzle supply enthalpy of 3.3MJ/kg and nozzle supply pressures of 32, 26 and 16MPa were performed at equivalence ratios of 0.0 and 0.8. The fuel-off lift coefficient remained constant but the thrust coefficient increased. This is attributed to a reduction in skin friction associated with longer lengths of laminar boundary layers as the Reynolds number was decreased. The measured fuel-off lift and thrust coefficients agreed with the predicted values to within the known test flow and force prediction uncertainties. Combustion did not occur at a nozzle supply pressure of 16MPa. This work has demonstrated that overall scramjet vehicle performance measurements (such as lift-to-drag ratio and shifts in centre-of-pressure) can be made in a free piston shock tunnel.

<p>This thesis aimed at studying mechanisms involved in control of tissue fluid homeostasis during inflammation.</p><p>The interstitial fluid pressure (P_IF) is of importance for control of tissue fluid balance. A lowering of P_IF <i>in vivo</i> will result in a transport of fluid from the circulation into the tissue, leading to edema. Loose connective tissues that surround blood vessels have an intrinsic ability to take up fluid and swell. The connective tissue cells exert a tension on the fibrous network of the tissues, thereby preventing the tissues from swelling. Under normal homeostasis, the interactions between the cells and the fibrous network are mediated by β1 integrins. Connective tissue cells are in this way actively controlling P_IF.</p><p>Here we show a previously unrecognized function for the integrin αVβ3, namely in the control of P_IF. During inflammation the β1 integrin function is disturbed and the connective tissue cells release their tension on the fibrous network resulting in a lowering of P_IF. Such a lowering can be restored by platelet-derived growth factor (PDGF) -BB. We demonstrated that PDGF-BB restored P_IF through a mechanism that was dependent on integrin αVβ3. This was shown by the inability of PDGF-BB to restore a lowered P_IF in the presence of anti-integrin β3 IgG or a peptide inhibitor of integrin αVβ3. PDGF-BB was in addition unable to normalize a lowered P_IF in β3 null mice. Furthermore, we demonstrated that extracellular proteins from <i>Streptococcus equi</i> modulated αVβ3-mediated collagen gel contraction. Because of the established concordance between collagen gel contraction <i>in vitro</i> and control of P_IF <i>in vivo</i>, a potential role for these proteins in control of tissue fluid homeostasis during inflammation could be assumed. Sepsis and septic shock are severe, and sometimes lethal, conditions. Knowledge of how bacterial components influence P_IF and the mechanisms for tissue fluid control during inflammatory reactions is likely to be of clinical importance in treating sepsis and septic shock.</p>