The topic of the diploma project is a design of a multipurpose building in a given city block structure which was prepared by KAM Brno in a local study plan "Southern Quarter - Trnitá - KAM Brno 2020" as a base material for future local zoning plan of Brno. The site is situated at corner of the block, in a proximity to a park along the river Svratka. The multipurpose building offers space for above-standard apartments as well as for office or commercial business. The massive cantilever created by the upper floors at the corner part is characteristic for the mass of the building and its opening the public space at the lower commercial floors.

Molecular dynamics simulation was used to study ion permeation through different membrane proteins embedded in a lipid bilayer (DMPC) with different saline solutions. The potential of mean force (PMF) for ion transport was obtained by umbrella sampling simulations. A revised MacKerell force field for tryptophan residues was studied using gramicidin A (gA) channel as a test model. The revised force field contribution to the Na+ PMF was consonant with the prediction from the experimental results, but in stark contrast to the prediction of the CHARMM force field, version 22, for the tryptophan side-chain. A new grid-based correction map algorithm by MacKerell group, called CMAP, was introduced into the CHARMM force field, version 31. The CMAP algorithm focused on optimizing phi, psi dihedral parameters for the peptide backbone. The CMAP corrections reduced the excessive translocation barrier. Decomposition demonstrated the reduction in the translocation barrier was due to effects on the K+ PMFH2O rather than on K+ PMFgA. The presence of negatively charged sulfonate group at the entrance and exit of the gA channel affected the depth and the location of the highly occupied sites. The negatively charged sulfonate group produced a strong attraction for the cations in the bulk towards the channel mouth. In the M2 transmembrane domain channel (M2-TMD), three M2-TMD structures were studied, differing only in whether the selectivity-filter (four His37 side-chains) was uncharged, +2 charged, or +3 charged. M2-TMD structural properties were compared with the structural properties of other models extracted from NMR and X-ray studies. The spontaneous cation and anion entry into the charged selectivity-filter was different from that into a neutral selectivity-filter. Cl- ions had a lower free-energy barrier in the selectivity-filter than either Na+ or NH4+ ions through the M2-TMD channel. NH4+ ions had a lower free-energy barrier in the selectivity-filter than Na+ ions. Based on accessible rotamer conformations, a revised conductance mechanism was proposed. In this conductance mechanism, the His37 side-chain functioned as an acceptor and donor group, whereas the Trp41 side-chain functioned as a carrying group.

This thesis describes the principles of making application which is able to set and control camera. The first part describes basic camera connections and it explains definition and specification of camera’s features. The second part of my thesis describes implementation of application, which can use more cameras to image preview, image acquisition and to simply set of camera’s features. To implement the applications we can use MATLAB and C++BUILDER uses opencv libraries.

Master thesis high-rack storage area spo - selected parts of ctp covers technological studies throughout the building, the construction division, building and engineering structures, preparation of normal technology, mechanical engineering time and financial plan. Also master thesis focuses more on building a major object of the building, which is the storage, dispatch and administrative part of high-rack storage area spo. Building on this subject is further developed such documentation and other documents that complement and prescribe requirements for faster, easier, safer, better and technologically accurate realization of this phase.

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The project solves a multifunctional Duma building in a vacant lot, contemplated the construction site is located in Carlsbad, in the street Vyhlíce. This is a protected site spa. Part of the project's layout and structural design of the house. It is a six-storey house with an attic and a basement floor. It is designed as a free-standing in the gap as the final house. The layout is divided into two complete units with their own input. There are spaces for business and residential units for permanent housing. Part of the living area are also room house equipment. Inputs to both parts are wheelchair accessible. The house is not wheelchair The house is designed as a brick building of brick masonry Porotherm the module dimensions of 250 (125) mm with reinforced concrete ceilings. Roofed by a hipped roof. The house is located on a private plot of 519 m2 built-up area of 221 m2. The land is gently sloping. The main orientation of the building to the cardinal's east and west. The south wall is adjacent to the neighboring house.