Les traitements actuels de l’arthrose ne sont pas étiologiques. Il est alors nécessaire de développer de nouveaux axes thérapeutiques. L’utilisation des cellules stromales mésenchymateuses (CSM) est prometteuse notamment en raison de leurs capacités immunomodulatrices. L’injection intra-articulaire des CSM présente quelques limites majeures qui pourraient être contournées par leur incorporation dans des biomatériaux. Cependant, le maintien des capacités immunomodulatrices des CSM après encapsulation n’a jamais été vérifié. Dans ce contexte, ce travail de thèse a consisté à encapsuler les CSM dans l’alginate et l’hydroxypropyl methylcellulose silanisée (HPMC-Si) puis à étudier leur biofonctionnalité. Dans un premier temps, une méthode de génération de particules d’HPMC-Si a été mise au point. Les particules d’alginate et d’HPMC-Si ont été caractérisées. La taille, les propriétés de diffusion et les propriétés mécaniques des particules fabriquées ont été analysées et comparées. Dans un second temps, les cellules ont été encapsulées dans les 2 matériaux puis caractérisées par une étude de leur viabilité et de leur prolifération pendant 1 mois. Enfin, le maintien des capacités immunomodulatrices des CSM après encapsulation a été investigué. Ces travaux de thèse ont permis de démontrer que : (i) les particules d’alginate et d’HPMC-Si possèdent des propriétés différentes ; (ii) les CSM encapsulées dans les 2 matériaux restent viables et (iii) l’encapsulation des CSM n’altère pas leurs capacités immunomodulatrices. Ces travaux de thèse sont ainsi encourageants pour une potentielle application des CSM encapsulées dans le traitement de l’arthrose. / Existing drug therapies for osteoarthritis (OA) provide, at best, symptomatic relief from pain and fail to prevent cartilage damage. Mesenchymal Stromal Cells (MSC) have generated interest since they secrete immunomodulatory factors. Direct injection of MSCs in OA suffers major limitations. To overcome these limitations, several studies have proposed to entrap MSC within biomaterials. However, their immunomodulatory properties after encapsulation have not been investigated. In this context, this work aimed to entrap MSC within spherical particles derived from alginate or from silanized hydroxypropyl methylcellulose (Si-HPMC) and to investigate their biofunctionality. First, a protocol of Si-HPMC particles generation was assessed. Alginate and Si-HPMC particles were characterized. Size, diffusion and mechanical properties of generated particles were studied and compared. Secondly, MSC were entrapped within alginate or Si- HPMC. Their viability and their ability to proliferate were evaluated for up to one month after encapsulation. Lastly, the biofunctionality of encapsulated MSC was investigated in order to harness their therapeutic properties for the treatment of OA. In summary, we have shown that: (i) alginate and Si-HPMC particles exhibit different properties; (ii) both alginate and Si-HPMC particles support MSC survival and (iii) MSC encapsulated in alginate or Si-HPMC are sensitive to pro-inflammatory cytokines and respond to this stimulation by increasing their secretion of bioactive factors. These findings are promising for a potential application of encapsulated MSC to OA treatment.

No description available.

Master of Science / Department of Animal Sciences and Industry / Robert Goodband / Five experiments were conducted to determine the effects of sorghum dried distiller’s grains with solubles (DDGS) on nursery pig performance, finishing pig performance and meat quality, and lactating sow and litter performance. Experiment 1 established a nutrient database and evaluated the quality and consistency of five ethanol plants in the Western Plains region. Each sample was analyzed for AA, DM, CP, crude fiber, crude fat, ash, NDF, ADF, Ca, P, trace minerals, GE, and starch. In addition, DE, ME, and NE were calculated. Experiments 2 and 3 evaluated the effects of sorghum DDGS on nursery pig performance. In Exp. 2, pigs fed sorghum- or corn-based diets performed similarly; however, as sorghum DDGS increased ADG decreased (linear, P < 0.01). A DDGS×grain source interaction (quadratic, P = 0.03) was observed for G:F. In corn-based diets, G:F was identical for pigs fed 0 to 30% DDGS, but worsened in pigs fed 45% DDGS. In sorghum-based diets, G:F was best for those fed 0% DDGS, but worsened at higher levels. In Exp. 3, pigs fed corn- or sorghum-based diets performed similarly; however, pigs fed DDGS gained less (P < 0.03) than those fed basal diets. Experiment 4 evaluated the effects of sorghum DDGS in sorghum- or corn-based diets on finishing pig growth performance, carcass characteristics, and fat quality. Increasing sorghum DDGS reduced (linear; P < 0.01) ADG and increased (linear; P < 0.01) backfat IV. Pigs fed the sorghum-based diet with 30% sorghum DDGS had decreased backfat IV (P < 0.01) than those fed the sorghum-based diet with corn DDGS. Experiment 5 evaluated the effects of sorghum DDGS on lactating sow and litter performance. Overall, ADFI increased in corn-based diets when DDGS were added, but decreased in sorghum-based diets resulting in a tendency (P < 0.08) for a DDGS × grain source interaction. Pig weaning weights were lower (P < 0.06) for sows fed diets containing DDGS compared with those fed the basal diets. Adding sorghum DDGS reduced pig performance, and sow lactation performance; therefore, its inclusion in swine diets needs to be evaluated on an income over feed cost basis.

Bio fuels are made from an extensive selection of fuels derived from biomass, including wood waste, agricultural wastes, and alcohol fuels. As a result of increased energy requirements, raised oil prices, and concern over greenhouse gas emissions from fossil fuels, bio fuels are acquiring increased public and scientific attention. The ethanol industry is booming and during the past several years, there has been an increase in demand for fuel ethanol and use of its co-products. To increase potential revenues from ethanol processing and its utilization, extensive research is proceeding in this field. In Western Canada, wheat is the primary raw material used in the production of ethanol by fermentation and distillers dried grains with solubles (DDGS) are one of the major co-products produced during this process. At present, the DDGS are generally sold as animal feed stock but with some alteration they could be used in other useful areas.<p> Densification of biomass and use of it for fuel like wood pellets, hay briquettes, etc. have been studied for many years and have also been commercialized. In this thesis, pellets made from distillers dried grains have been investigated. DDGS were obtained from Noramera Bioenergy Corp. and Terra Grain Fuels Ltd. Before transforming them into pellets, they were characterized on the basis of physical and chemical properties. A California pilot-scale mill (with and without steam conditioning) was used for pelleting the distillers grains with solubles.<p> A full factorial design with two levels of moisture content (i.e., 14 and 15.5% (w.b.)), hammer mill screen size (i.e., 3.2 and 4.8 mm) and temperature (i.e., 90 and 100°C) was used to determine the effects of these three factors on the pellet properties made from Noramera Bioenergy Corp., without steam conditioning. Different levels of moisture content were used for the pellets made from Terra Grain Fuels Ltd. (i.e., 11.5 and 13.09% (w.b.)), with steam conditioning. The initial moisture contents of the DDGS were 12.5 and 13.75% (w.b.) from Noramera and Terra Grain, respectively. The moisture content of DDGS grinds ranged from 11.6 to 12.03% (w.b.) for the Noramera samples, and from 11.5 to 13.09% (w.b.) for Terra Grain DDGS. The moisture content decreased with a decrease in the hammer mill screen size.<p> The use of a smaller screen size achieved an increase in both the bulk and particle densities of the DDGS. The coefficient of internal friction was almost the same for both samples but cohesion was higher in Noramera samples (8.534 kPa). The DDGS obtained from Noramera Bioenergy Corp. contained dry matter (91.40%), crude fibre (4.98%), crude protein (37.41%), cellulose (10.75%), hemi-cellulose (21.04%), lignin (10.50%), starch (3.84%), fat (4.52%) and ash (5.16%); whereas the samples obtained from Terra Grain Fuels contained dry matter (87.69%), crude fibre (7.33%), crude protein (32.43%), cellulose (10.81%), hemi-cellulose (27.45%), lignin (4.37%), starch (4.18%), fat (6.37%) and ash (4.50%).<p> The combustion energy of the Noramera samples was 19.45 MJ/kg at a moisture content of 8.6% (w.b.) whereas the combustion energy of Terra Grain samples was 18.54 MJ/kg at 12.31% (w.b.) moisture content.<p> The durability of the pellets increased as the screen size decreased which is likely due to the fact that a smaller screen size produces more fine particles. This fill voids in the pellets and, hence, makes them more durable.<p> The length of the pellets produced from Noramera DDGS increased with a decrease in moisture content possibly because pellets formed at higher moisture content absorb less moisture. Therefore, the length does not increase as much. Lateral expansion occurred most with higher temperature and lower moisture content and with lower temperature and higher moisture content. The length to diameter ratio of the pellets followed the same trend as the change in pellet length. The length of the pellets produced from Terra Grain also increased with a decrease in moisture content. The lateral expansion increased with increase in screen size and moisture content and also, with decrease in moisture content and increase in temperature. The length to diameter ratio increased with decrease in screen size and moisture content, similar to the change in pellet length. The highest bulk density of Noramera pellets resulted from smaller screen size and lower moisture. The particle density increased with a decrease in screen size and an increase in moisture content. The highest bulk density of Terra Grain pellets occurred with an increase in temperature and decrease in moisture content. The highest particle density occurred with an increase in temperature and decrease in screen size.<p> The pellet hardness increased with a decrease in moisture content and screen size did not have any significant effect. The Terra Grain pellets were harder because they were subjected to steam conditioning. Steam conditioning helps to increase the hardness.<p> The pellet durability increased with a decrease in screen size and increase in moisture content. The steam conditioning also caused the higher durability in the Terra Grain pellets. In terms of moisture absorption, the only significant factor was moisture content. Pellets with lower moisture content absorbed more moisture.<p> The ash content values of pellets were higher in Noramera samples than in Terra Grain samples because of high moisture content in Noramera samples. The combustion energy of the Noramera pellets was higher than the Terra Grain pellets because of the high percentage of dry matter and lignin present in Noramera samples. The emission results for both the sample pellets were similar. When the DDGS pellets were compared to wood pellets, emission of nitrous oxide was lower for wood whereas, carbon dioxide was higher.

The main objective of this project was to investigate the effects of the type of dried distillers grains with solubles (wheat DDGS, corn DDGS, and blend DDGS (eg. wheat:corn = 70:30)) and bio-ethanol plant origin on the nutrient variation and availability in ruminants. In addition, DDGS products were studied as opposed to their parental grains. The project was divided into the several following studies. In Study 1, we studied the nutritive value of DDGS products in terms of (1) chemical profiles, (2) protein and carbohydrate sub-fractions associated with different degradation rates, and (3) digestible component nutrients and energy values using the NRC 2001-chemical approach and the in situ assay-biological approach. Also, we tested the validity of acid detergent insoluble crude protein (ADICP) and acid detergent lignin (ADL) to predict the potential degradability of DDGS. Due to starch fermentation in the ethanol process, the chemical components in DDGS became approximately threefold more concentrated than in feedstock grains. Slowly degraded protein (PB3) and unavailable protein (PC) increased in DDGS, indicating a decrease in the overall protein degradability in the rumen. Intermediately degraded protein (PB2) was higher for corn DDGS than for wheat DDGS and blend DDGS (54.2 vs. 27.7 vs. 30.8 %CP), while PB3 was higher for wheat DDGS and blend DDGS (29.9 vs. 51.2 vs. 53.2 %CP). Mainly as a result of differing heat conditions, PC differed significantly between wheat DDGS originated at different bio-ethanol plants (0.7 vs. 7.6 %CP). The prediction of truly digestible CP (tdCP) and NDF (tdNDF) differed between the NRC 2001-chemical approach and the in situ assay-biological approach; however, both approaches reported similar energy values. These values were the highest for corn DDGS (DE3X: 3.9 Mcal kg-1), followed by blend DDGS (DE3X: 3.6 Mcal kg-1), and wheat DDGS (DE3X: 3.4 Mcal kg-1). Corn DDGS was superior to corn, wheat DDGS was similar to wheat and corn, and blend DDGS was similar to corn. No significant differences in energy values were reported between bio-ethanol plants. ADICP was not an accurate indicator of the potential degradability of protein in DDGS samples, while ADL seemed to be an acceptable indicator of the potential degradability of DM (r = -0.87; P<0.01), CP (r = -0.89; P<0.01), and NDF (r = -0.82; P<0.01) in wheat DDGS samples incubated in rumen during 48 h.<p> In Study 2, we studied the ruminal and intestinal digestion profiles and the hourly effective rumen degradation ratios between nitrogen (N) and energy. The results showed a reduction in the effective degradability of DM (EDDM), OM (EDOM) and CP (EDCP) of wheat DDGS relative to wheat; however, corn DDGS remained the same as corn. The effective degradability of NDF (EDNDF) did not vary between the DDGS samples and feedstock grains. Among DDGS types, EDDM ranged from 52.4 to 57.7 %, EDOM from 46.4 to 53.5 %DM, and EDCP from 34.0 to 45.6 %CP, being higher as the proportion of wheat in feedstock increased. No significant differences in EDDM, EDOM, EDCP and EDNDF for wheat DDGS were detected between the different bio-ethanol plants. The hourly effective degradability ratios between N and energy indicated a potential excess of N in rumen when DDGS samples were evaluated as single ingredient. This excess increased as the proportion of wheat in feedstock increased. Estimated intestinal digestibility of rumen bypass protein (IDP) was similar between wheat and wheat DDGS, but higher in corn DDGS than in corn. Blend DDGS had the highest IDP (93.9 %RUP). Due to the significantly different PC sub-fraction found in wheat DDGS originated at the different bio-ethanol plants, a large but numerical difference was detected in IDP (89.4 vs. 75.9 %RUP).<p> In Study 3, we used both the DVE/OEB System and the NRC 2001 Model to reveal the metabolic characteristics of DDGS protein and predict the protein supply to dairy cattle. The two models showed higher protein values (DVE or MP) for DDGS samples than for feedstock grains. The higher IDP for blend DDGS largely contributed to the higher protein value relative to wheat DDGS and corn DDGS (MP: 277 vs. 242 vs. 250 g kg-1 DM). Similarly, protein values differed significantly between the bio-ethanol plants mainly as a result of the numerical but large difference in IDP (MP: 272 vs. 223 g kg-1 DM). According to the two models, the degraded protein balance for DDGS products was higher than in the parental grains. Wheat DDGS showed the highest potential N excess (DBPNRC: 78 g kg-1 DM). For corn DDGS, however, the DVE/OEB System suggested a potential N excess (11 g kg-1 DM) while the NRC 2001 Model exhibited a potential N deficiency (-12 g kg-1 DM). The degraded protein balance for wheat DDGS was similar between the different bio-ethanol plants.<p> In conclusion, the chemical and biological characteristics of DDGS varied among types and between wheat DDGS samples manufactured at the different bio-ethanol plants. Thus, it is inappropriate to assume fixed values for the nutritive value of DDGS without considering factors such as type of grain used and bio-ethanol plant origin. Further research with higher number of samples will help to clarify the use of the chemical profile to predict energy values and the potential degradability of DDGS.

Bio fuels are made from an extensive selection of fuels derived from biomass, including wood waste, agricultural wastes, and alcohol fuels. As a result of increased energy requirements, raised oil prices, and concern over greenhouse gas emissions from fossil fuels, bio fuels are acquiring increased public and scientific attention. The ethanol industry is booming and during the past several years, there has been an increase in demand for fuel ethanol and use of its co-products. To increase potential revenues from ethanol processing and its utilization, extensive research is proceeding in this field. In Western Canada, wheat is the primary raw material used in the production of ethanol by fermentation and distillers dried grains with solubles (DDGS) are one of the major co-products produced during this process. At present, the DDGS are generally sold as animal feed stock but with some alteration they could be used in other useful areas.<p> Densification of biomass and use of it for fuel like wood pellets, hay briquettes, etc. have been studied for many years and have also been commercialized. In this thesis, pellets made from distillers dried grains have been investigated. DDGS were obtained from Noramera Bioenergy Corp. and Terra Grain Fuels Ltd. Before transforming them into pellets, they were characterized on the basis of physical and chemical properties. A California pilot-scale mill (with and without steam conditioning) was used for pelleting the distillers grains with solubles.<p> A full factorial design with two levels of moisture content (i.e., 14 and 15.5% (w.b.)), hammer mill screen size (i.e., 3.2 and 4.8 mm) and temperature (i.e., 90 and 100°C) was used to determine the effects of these three factors on the pellet properties made from Noramera Bioenergy Corp., without steam conditioning. Different levels of moisture content were used for the pellets made from Terra Grain Fuels Ltd. (i.e., 11.5 and 13.09% (w.b.)), with steam conditioning. The initial moisture contents of the DDGS were 12.5 and 13.75% (w.b.) from Noramera and Terra Grain, respectively. The moisture content of DDGS grinds ranged from 11.6 to 12.03% (w.b.) for the Noramera samples, and from 11.5 to 13.09% (w.b.) for Terra Grain DDGS. The moisture content decreased with a decrease in the hammer mill screen size.<p> The use of a smaller screen size achieved an increase in both the bulk and particle densities of the DDGS. The coefficient of internal friction was almost the same for both samples but cohesion was higher in Noramera samples (8.534 kPa). The DDGS obtained from Noramera Bioenergy Corp. contained dry matter (91.40%), crude fibre (4.98%), crude protein (37.41%), cellulose (10.75%), hemi-cellulose (21.04%), lignin (10.50%), starch (3.84%), fat (4.52%) and ash (5.16%); whereas the samples obtained from Terra Grain Fuels contained dry matter (87.69%), crude fibre (7.33%), crude protein (32.43%), cellulose (10.81%), hemi-cellulose (27.45%), lignin (4.37%), starch (4.18%), fat (6.37%) and ash (4.50%).<p> The combustion energy of the Noramera samples was 19.45 MJ/kg at a moisture content of 8.6% (w.b.) whereas the combustion energy of Terra Grain samples was 18.54 MJ/kg at 12.31% (w.b.) moisture content.<p> The durability of the pellets increased as the screen size decreased which is likely due to the fact that a smaller screen size produces more fine particles. This fill voids in the pellets and, hence, makes them more durable.<p> The length of the pellets produced from Noramera DDGS increased with a decrease in moisture content possibly because pellets formed at higher moisture content absorb less moisture. Therefore, the length does not increase as much. Lateral expansion occurred most with higher temperature and lower moisture content and with lower temperature and higher moisture content. The length to diameter ratio of the pellets followed the same trend as the change in pellet length. The length of the pellets produced from Terra Grain also increased with a decrease in moisture content. The lateral expansion increased with increase in screen size and moisture content and also, with decrease in moisture content and increase in temperature. The length to diameter ratio increased with decrease in screen size and moisture content, similar to the change in pellet length. The highest bulk density of Noramera pellets resulted from smaller screen size and lower moisture. The particle density increased with a decrease in screen size and an increase in moisture content. The highest bulk density of Terra Grain pellets occurred with an increase in temperature and decrease in moisture content. The highest particle density occurred with an increase in temperature and decrease in screen size.<p> The pellet hardness increased with a decrease in moisture content and screen size did not have any significant effect. The Terra Grain pellets were harder because they were subjected to steam conditioning. Steam conditioning helps to increase the hardness.<p> The pellet durability increased with a decrease in screen size and increase in moisture content. The steam conditioning also caused the higher durability in the Terra Grain pellets. In terms of moisture absorption, the only significant factor was moisture content. Pellets with lower moisture content absorbed more moisture.<p> The ash content values of pellets were higher in Noramera samples than in Terra Grain samples because of high moisture content in Noramera samples. The combustion energy of the Noramera pellets was higher than the Terra Grain pellets because of the high percentage of dry matter and lignin present in Noramera samples. The emission results for both the sample pellets were similar. When the DDGS pellets were compared to wood pellets, emission of nitrous oxide was lower for wood whereas, carbon dioxide was higher.

The main objective of this project was to investigate the effects of the type of dried distillers grains with solubles (wheat DDGS, corn DDGS, and blend DDGS (eg. wheat:corn = 70:30)) and bio-ethanol plant origin on the nutrient variation and availability in ruminants. In addition, DDGS products were studied as opposed to their parental grains. The project was divided into the several following studies. In Study 1, we studied the nutritive value of DDGS products in terms of (1) chemical profiles, (2) protein and carbohydrate sub-fractions associated with different degradation rates, and (3) digestible component nutrients and energy values using the NRC 2001-chemical approach and the in situ assay-biological approach. Also, we tested the validity of acid detergent insoluble crude protein (ADICP) and acid detergent lignin (ADL) to predict the potential degradability of DDGS. Due to starch fermentation in the ethanol process, the chemical components in DDGS became approximately threefold more concentrated than in feedstock grains. Slowly degraded protein (PB3) and unavailable protein (PC) increased in DDGS, indicating a decrease in the overall protein degradability in the rumen. Intermediately degraded protein (PB2) was higher for corn DDGS than for wheat DDGS and blend DDGS (54.2 vs. 27.7 vs. 30.8 %CP), while PB3 was higher for wheat DDGS and blend DDGS (29.9 vs. 51.2 vs. 53.2 %CP). Mainly as a result of differing heat conditions, PC differed significantly between wheat DDGS originated at different bio-ethanol plants (0.7 vs. 7.6 %CP). The prediction of truly digestible CP (tdCP) and NDF (tdNDF) differed between the NRC 2001-chemical approach and the in situ assay-biological approach; however, both approaches reported similar energy values. These values were the highest for corn DDGS (DE3X: 3.9 Mcal kg-1), followed by blend DDGS (DE3X: 3.6 Mcal kg-1), and wheat DDGS (DE3X: 3.4 Mcal kg-1). Corn DDGS was superior to corn, wheat DDGS was similar to wheat and corn, and blend DDGS was similar to corn. No significant differences in energy values were reported between bio-ethanol plants. ADICP was not an accurate indicator of the potential degradability of protein in DDGS samples, while ADL seemed to be an acceptable indicator of the potential degradability of DM (r = -0.87; P<0.01), CP (r = -0.89; P<0.01), and NDF (r = -0.82; P<0.01) in wheat DDGS samples incubated in rumen during 48 h.<p> In Study 2, we studied the ruminal and intestinal digestion profiles and the hourly effective rumen degradation ratios between nitrogen (N) and energy. The results showed a reduction in the effective degradability of DM (EDDM), OM (EDOM) and CP (EDCP) of wheat DDGS relative to wheat; however, corn DDGS remained the same as corn. The effective degradability of NDF (EDNDF) did not vary between the DDGS samples and feedstock grains. Among DDGS types, EDDM ranged from 52.4 to 57.7 %, EDOM from 46.4 to 53.5 %DM, and EDCP from 34.0 to 45.6 %CP, being higher as the proportion of wheat in feedstock increased. No significant differences in EDDM, EDOM, EDCP and EDNDF for wheat DDGS were detected between the different bio-ethanol plants. The hourly effective degradability ratios between N and energy indicated a potential excess of N in rumen when DDGS samples were evaluated as single ingredient. This excess increased as the proportion of wheat in feedstock increased. Estimated intestinal digestibility of rumen bypass protein (IDP) was similar between wheat and wheat DDGS, but higher in corn DDGS than in corn. Blend DDGS had the highest IDP (93.9 %RUP). Due to the significantly different PC sub-fraction found in wheat DDGS originated at the different bio-ethanol plants, a large but numerical difference was detected in IDP (89.4 vs. 75.9 %RUP).<p> In Study 3, we used both the DVE/OEB System and the NRC 2001 Model to reveal the metabolic characteristics of DDGS protein and predict the protein supply to dairy cattle. The two models showed higher protein values (DVE or MP) for DDGS samples than for feedstock grains. The higher IDP for blend DDGS largely contributed to the higher protein value relative to wheat DDGS and corn DDGS (MP: 277 vs. 242 vs. 250 g kg-1 DM). Similarly, protein values differed significantly between the bio-ethanol plants mainly as a result of the numerical but large difference in IDP (MP: 272 vs. 223 g kg-1 DM). According to the two models, the degraded protein balance for DDGS products was higher than in the parental grains. Wheat DDGS showed the highest potential N excess (DBPNRC: 78 g kg-1 DM). For corn DDGS, however, the DVE/OEB System suggested a potential N excess (11 g kg-1 DM) while the NRC 2001 Model exhibited a potential N deficiency (-12 g kg-1 DM). The degraded protein balance for wheat DDGS was similar between the different bio-ethanol plants.<p> In conclusion, the chemical and biological characteristics of DDGS varied among types and between wheat DDGS samples manufactured at the different bio-ethanol plants. Thus, it is inappropriate to assume fixed values for the nutritive value of DDGS without considering factors such as type of grain used and bio-ethanol plant origin. Further research with higher number of samples will help to clarify the use of the chemical profile to predict energy values and the potential degradability of DDGS.

No description available.

ABSTRACT Two experiments were conducted to determine the net energy (NE) of wheat-corn distillers dried grains with solubles (wcDDGS) using the comparative slaughter (CS), the indirect calorimetry (IC) and the chemical composition (CH) methods. Based on the CS method, NE values of 2,407, and 2,424 kcal/kg DM were obtained for wcDDGS included at 15% and 30%, respectively. For the IC method, the NE values of 2,407, and 2,403 kcal/kg DM were obtained for wcDDGS included at 15% and 30%, respectively; corresponding values for the CH method were 2,536 and 2,197 kcal/kg DM, respectively. It is concluded that NE value of wcDDGS ranges from 2,367 kcal/kg DM to 2,416 kcal/kg DM depending on the method used. As the values obtained from the various methods were not different, the average NE value for the wcDDGS evaluated was 2,396 ± 25.71 kcal/kg DM.

ABSTRACT Two experiments were conducted to determine the net energy (NE) of wheat-corn distillers dried grains with solubles (wcDDGS) using the comparative slaughter (CS), the indirect calorimetry (IC) and the chemical composition (CH) methods. Based on the CS method, NE values of 2,407, and 2,424 kcal/kg DM were obtained for wcDDGS included at 15% and 30%, respectively. For the IC method, the NE values of 2,407, and 2,403 kcal/kg DM were obtained for wcDDGS included at 15% and 30%, respectively; corresponding values for the CH method were 2,536 and 2,197 kcal/kg DM, respectively. It is concluded that NE value of wcDDGS ranges from 2,367 kcal/kg DM to 2,416 kcal/kg DM depending on the method used. As the values obtained from the various methods were not different, the average NE value for the wcDDGS evaluated was 2,396 ± 25.71 kcal/kg DM.