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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

O emprego da metodologia de superfície de resposta no desenvolvimento de um novo produto simbiótico, fermentado com Enterococcus faecium CRL 183 e Lactobacillus helveticus ssp jugurti 416, à base de extratos aquosos de soja e de Yacon (Smallanthus sonchifolius) /

Silveira, Nadiége Dourado Pauly. January 2009 (has links)
Resumo: O desenvolvimento de alimentos funcionais tem aumentado mundialmente devido a seus compostos bioativos que diminuem o risco de doenças e promovem a saúde. Baseado nisso, a indústria e a comunidade científica estão, cada vez mais, buscando ingredientes que possam conferir tais características aos alimentos. A soja, além de ser importante fonte protéica, possui fibras, isoflavonas, oligossacarídeos com potencial prebiótico (rafinose e estaquinose), vitaminas e minerais. As raízes tuberosas de yacon (Smallanthus sonchifolius) possuem grande quantidade de frutooligossacarídeos do tipo inulina, um componente não digerível pelo nosso organismo, com função de auxiliar na seleção de determinadas bactérias da microbiota intestinal, principalmente as bifidobactérias, possuindo, portanto, efeito prebiótico. O objetivo do presente estudo foi o de verificar, através da metodologia da superfície de resposta, as melhores condições para a utilização simultânea de extratos aquosos de soja e de yacon no desenvolvimento de um novo produto fermentado por um cultivo probiótico constituído por Enterococcus faecium CRL 183 e Lactobacillus helveticus ssp jugurti 416. Para tanto, as variáveis independentes foram representadas pelo teor protéico fornecido pelo extrato hidrossolúvel de soja (g/L) e pelo extrato aquoso de yacon (%). As variáveis dependentes (respostas) foram obtidas através do teste de aceitação sensorial ("sabor" e "impressão global"). A otimização conjunta das variáveis apontou a formulação 7 (1,74 g/L de teor protéico fornecido pelo extrato de soja e 25,86% de extrato de yacon) do planejamento experimental como sendo a mais adequada. No entanto, as formulações 9 e 10 (ambas contendo 1,74 g/L de teor protéico fornecido pelo extrato de soja e 40% de extrato de yacon) apresentaram valores de aceitação que não diferiram significativamente... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: World-wide the development of functional food has been increased, due to its bioactive components which decrease the risk of getting some illnesses and promote health. Based on that, the industry and the scientific population are more and more searching for ingredients that may confer such characteristics to food. The soy, besides being an important proteic source, has fibers and isoflavones, oligosaccharides with potential prebiotic (raffinose and stachyose), vitamins and minerals. The yacon tuber (Smallanthus sonchifolius) is a source of fructooligosaccharides, type inulin, undigestible components by our organism that helps in the selection and development of some bacteria of intestinal microbiota, mainly the bifidobacteria, which means that yacon has prebiotic effect. The aim of this study was to check, through Response Surface Methodology, the optimum conditions to use, simultaneously, the soy and yacon watery extracts in the development of a new fermented product with the probiotic cultive constituted by Enterococcus faecium CRL 183 and Lactobacillus helveticus ssp jugurti 416. Therefore, the independent variables were represented by the protein of soymilk (g/L) and the yacon extract (%). The dependent variables (responses) were obtained through the acceptance test ("taste" and "overall acceptability"). The joint optimization of the variables pointed out the 7th formulation (1.74 g/L of soymilk protein and 25.86% of yacon extract) of the factorial design as the most adequate. However, the 9th and 10th formulations (both containing 1.74 g/L of soymilk protein and 40% of yacon extract) presented acceptance scores significantly equal to the 7th treatment. The optimized product was submitted to the shelf-life determination, and it was presented in conditions of consuming in the course of 21 days at 5 ºC and with viability of the probiotic cultive among... (Complete abstract click electronic access below) / Orientador: Elizeu Antonio Rossi / Coorientador: Dionisio Borsato / Banca: Daniela Cardoso Umbelino Cavallini / Banca: Eliane Maria Ravasi Stéfano Simionato / Mestre

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