Thesis (Ph. D.)--Virginia Polytechnic Institute and State University, 1991. / Vita. Abstract. Includes bibliographical references (leaves 19-30). Also available via the Internet.

Thesis (M.Sc.). / Written for the Dept. of Animal Science. Title from title page of PDF (viewed 2008/01/16). Includes bibliographical references.

Thesis (M.S.)--University of Missouri-Columbia, 2008. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on August 12, 2009) Includes bibliographical references.

Thesis (Ph.D.) - University of Queensland, 2004. / Includes bibliography.

Submitted in partial fulfillment of the requirements for the Degree of Master of Technology: Biotechnology, Durban Institute of Technology, 2003. / South Africa produces 190 000 tonnes of soybean per annum. Seed producing companies require knowledge of the diversity of the germplasm to produce hybrids that will be competitive in local and overseas markets. Furthermore, they need to ascertain the presence/absence of the anti-nutritional factor, Kunitz trypsin inhibitor protein. Currently, seed producing companies plant the seed and wait for the grow-out in order to select desirable traits. This process is time-consuming, tedious and does not necessarily ensure the selection of the best genetic stability as it is based on phenotypic expression alone. This study was undertaken to evaluate a molecular method to determine the genetic diversity among soybean parent lines and optimize a method which can be used to evaluate seeds for the Kunitz trypsin inhibitor protein / M

Five soymilk quality-related characteristics were investigated as affected by different grinding, heating, extraction methods and varieties. The five characteristics are (1) protein and solid recovery, (2) trypsin inhibitor activity, (3) antioxidant compounds and antioxidant capacity, (4) soy odor, and (5) isoflavone content and profile. The two varieties were Prosoy and black soybeans. The results show that significant differences existed among the three grinding methods (ambient grinding, cold grinding, and hot grinding). Ambient grinding gave the best protein and solid recoveries. Hot grinding showed the best results for the other four parameters. Cold grinding gave the poorest performance, with the exception of the odor profile. The three heating methods (traditional stove cooking, one-phase UHT, two-phase UHT) also resulted in significant differences in the chemical compounds and properties of the soymilk. In many cases, the effects of heating methods were closely related to grinding methods and varieties. Our results clearly demonstrated that a UHT processor equipped with a vacuum chamber was a very efficient way to reduce or eliminate some undesirable soy odors, especially in conjunction with hot grinding. Our results also demonstrated that many complex reactions occurred during thermal treatment. Because of different seed characteristics, the two different varieties behaved differently during processing. For both varieties, extraction with okara washing water from last batch (Method #2) gave the highest solid and protein recoveries.

The initial interaction of vesicle and the target membrane prior to their fusion is called vesicle tethering, a process mediated by an octameric protein complex called the exocyst. The exocyst connects vesicles and binds them to phosphatidylinositol 4, 5-biphosphate (PI (4,5) P2), located on the plasma membrane. The exocyst complex is located at the target site, helping to prepare the soluble N-ethylmaleimide-sensitive fusion protein attachment protein (SNAP) receptor (SNARE) for docking and subsequent release of vesicular contents after fusion. The importance of the exocyst in cellular processes is inevitable since it performs central roles in exocytosis thereby inducing SNARE-mediated membrane fusion. The study presented here is concentrated on the role of exocyst genes during the defense response in Glycine max (soybean) against the plant- parasitic nematode Heterodera glycines known as the soybean cyst nematode (SCN). Using developmental genomics analysis, G. max root cells that have been induced by H. glycines through their pathogenic activities to develop into nurse cells known as a syncytium have been isolated by laser capture microdissection (LCM). RNA isolated from these cells undergoing resistant reactions in two different G. max genotypes have been used in gene expression profiling experiments that have led to the identification of the genes employed in this analysis. The results demonstrate the involvement of exocyst components in the defense process that G. max has toward H. glycines. Related studies also show the involvement of RPM1-INTERACTING PROTEIN 4 (RIN4) functioning in this defense process.

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