Defatted soybean was subjected to isolation procedures as follows: NaOH extraction/acid precipitation (NaOH-IE); water extraction/acid precipitation (H2O-IE); NaOH extraction/cryoprecipitation (NaOH-CP) and citric acid extraction/cryoprecipitation (CA-CP). NaOH-CP and CA-CP each resulted in precipitation of one protein mainly consisting of glycinin and beta-conglycinin, respectively. The proteins were subjected to polyacrylamide gel electrophoresis (PAGE), SDS-PAGE, size exclusion-chromatography (SEC) and differential scanning calorimetry (DSC) for characterization. PAGE, SDS-PAGE, SEC and DSC confirmed the homogeneity of NaOH-CP. The gelation properties and structural characteristics of NaOH-CP were investigated; the minimum protein concentration required for formation of a thermally-induced gel from NaOH-CP was 8%. With cold-set gelation, in the presence of 30 mM CaCl2, the minimum protein concentration required for formation of a self-supporting gel was 4%. At 100 mM CaCl 2, cold-set gelation of 10% protein concentration gave gel of strength 7.4 N compared to the 7.8 N gel strength obtained with thermally-induced gel at 16% protein concentration. The effects of heating on the NaOH-CP structure, in the presence of varying concentrations of NaCl and reducing agents, were examined by Fourier transform infrared (FTIR) spectroscopy and DSC. FTIR spectroscopy revealed that at 25°C the NaOH-CP protein showed four bands at 1633, 1650, 1667, and 1689 cm-1. At 85°C, the temperature associated with gelation, the four bands decreased in intensity with the simultaneous increase in the intensity of a band at 1644 cm-1 as result of the protein unfolding, furthermore two new bands at 1616 and 1685 cm -1 appeared. The latter two bands are attributed to intermolecular mu-sheet formed during gelation. The NaOH-CP did not exhibit any aggregation band between 25--100°C in the presence of NaCl. The DSC studies revealed that the protein denatured at approximately 102°

[Truncated abstract] Some plants, such as legumes, are able to use atmospheric nitrogen as a nitrogen source due to the nitrogen-fixing bacteria residing in specialised root structures called nodules. The exchange of carbon and nitrogen between the host plant (legume) and the nitrogen- fixing micro-symbiont is vital for biological nitrogen fixation. In particular, transport of C4-dicarboxylates, mainly malate, from the plant to the micro-symbiont, and the reverse transport of fixed nitrogen in the form of ammonium are essential for symbiotic nitrogen fixation. In the legume nodule, the symbiosome membrane (SM) surrounds the bacteroid and all exchanges of metabolites and nutrients that occur between the plant and the micro-symbiont must cross this membrane. Recently it has been established that cycling of amino acids across the SM is also critical for optimal symbiotic nitrogen fixation. Therefore to fully understand this agriculturally significant phenomenon, the mechanisms facilitating these exchanges need to be investigated. The major aim of this study was to increase the understanding of nutrient exchange within the nodule at the molecular level by isolating and characterising genes encoding transporters responsible for malate and amino acids transport in soybean (Glycine max, L.), with particular interest in genes significantly or highly expressed in nodules. A combination of molecular and biochemical techniques was used to achieve this. ... Southern blot analysis showed that a small gene family of up to five members encodes these proteins in soybean. A full-length cDNA, designated GmAAP5, was isolated that encodes a novel, putative amino acid transporter. Molecular characterisation of this cDNA and that of GmAAP1 (GenBank Accession no: AY029352), a previously identified putative amino acid transporter gene, was done. Expression analyses showed relatively high expression of GmAAP5 in soybean nodules compared to that in leaf and root tissues, while GmAAP1 showed uniformly high expression in root, leaf and nodule tissues. Phylogenetic analysis of the deduced amino acid sequences of known functional AAPs from dicotyledonous plants revealed that GmAAP1 is most closely related to AAP2 from V. faba, while GmAAP5 is more closely related to AAPs from non-leguminous plants than from leguminous plants. Based on the functional characterisation of the AAPs with which GmAAP1 and GmAAP5 cluster, it is likely that both transporters are neutral and acidic amino acid transporters within the AAP subfamily.

Thesis (M.S.)--Mississippi State University. Department of Plant and Soil Sciences. / Title from title screen. Includes bibliographical references.

Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 116-121). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 116-121). Also available on the Internet.

The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on September 10, 2009). Thesis advisor: Joseph Parcell. Includes bibliographical references.

Thesis (doctoral)--Rijksuniversiteit te Utrecht, 1983. / Summary also in Dutch. Vita and acknowledgements in Dutch. Additional references and errata slip inserted. Includes bibliographical references (p. 75-79).

Thesis (Ph.D.) - University of Queensland, 2005. / Includes bibliography.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Includes bibliographical references (p. 161-180).

Made available in DSpace on 2014-06-11T19:25:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-06-27Bitstream added on 2014-06-13T19:52:54Z : No. of bitstreams: 1 corbo_e_me_jabo.pdf: 183600 bytes, checksum: 92b9b2f8b4062ac8366cd8b8a5c3a3ad (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / A soja transgênica MON 87701 × MON 89788 com Cry1Ac foi comparada com a soja isogênica não transgênica (M 8329) e soja RR (M 8360 RR) resistente ao herbicida glifosato no controle destes insetos, sob condições de casa-de-vegetação. Adotou-se o delineamento de blocos casualizados em parcelas subdividas com 4 repetições, sendo que os níveis de infestação corresponderam aos tratamentos primários, enquanto os genótipos de soja corresponderam aos tratamentos secundários. A soja foi cultivada em vasos plásticos sob gaiola telada. Os tratamentos primários para o ensaio com A. gemmatalis foram a) sem infestação; b) baixa infestação com liberação de 64,75+66,75 (fêmea+macho) adultos/parcela e c) alta infestação com liberação de 317,25+302,5 (fêmea+macho) adultos/parcela; enquanto para P. includens foram a) baixa infestação com liberação de 55,25+58,55 (fêmea+macho) adultos/parcela e b) alta infestação com liberação de 213,25+196,5 (fêmea+macho) adultos/parcela. Os tratamentos secundários foram soja MON 87701 × MON 89788; soja isogênica não transgênica; soja RR. Os resultados indicaram que a soja MON 87701 × MON 89788 foi altamente eficiente no controle da lagarta-da-soja e da falsa-medideira. A soja isogênica não transgênica (M 8329) e soja RR (M 8360 RR) não tiveram qualquer efeito nas populações de ambas as lagartas. Os adultos não discriminaram a soja geneticamente modificada e portanto não tiveram preferêncica para oviposição / The transgenic soybean MON 87701 × MON 89788 with Cry1Ac was compared to the isogenic non-transgenic soybeans (M 8329) and RR soybean (M 8360 RR) resistant to glyphosate in the control of the these pests, under greenhouse conditions. Split plot design with four randomized blocks (replications) and infestation levels corresponded to the primary treatment, together with the soybean genotypes corresponded to the secondary treatments was adopted in this study. Soybeans were planted in plastic pots under screened cage. For A. gemmatalis, the primary treatments were a) non-infested plants b) low infestation in which 64.75 +66.75 (female + male) adult/plot were releases c) high infestation in which 317.25 +302.5 (female + male) adult/plot were released; whereas, for P. includens the treatments were a) low infestation with 55.25 +58.55 (female + male) adult/plot and b) high infestation with 213.25 +196.5 (female + male) adult/plot released. The secondary treatments were soybean MON 87701 × MON 89788; isogenic non-transgenic soybean and RR soybean. The results indicated that soybean MON 87701 × MON 89788 was highly effective in controlling both velvetbean caterpillar and soybean looper. Adults of both insects were not able to discriminate the genetically modified soybean, therefore they did not show oviposition preference