Former researches provide evidence that invasive species could alter ecosystem’s components, threaten native species and cause economic losses in southern forest lands. The objective of the project is to explore significant driving factors and develop geospatial models for monitoring, predicting and mapping the extent and conditions of major invasive species. In the study area, 16 invasive species were classified into four groups: regionally spreading species, regionally establishing species, locally spreading species and regionally colonizing species by population size and spatial characteristics. According to local Moran’s I, spatial autocorrelation existed in 16 invasive species. Autologistic model and simultaneous autoregressive model were employed to explore the relationships between spatial distribution and a set of indentified variables for Chinese privet, kudzu, Nepalese browntop and tallow tree at plot and county levels. The project showed that human-caused disturbances and forest types were significantly related to the spatial distribution of four invasive species in different scales.

Aquatic plants (macrophytes) are important components of freshwater ecosystems and serve numerous purposes, physical and biological, that help to structure aquatic communities. Although macrophytes represent an essential component of stable aquatic communities, invasive macrophytes may negatively alter ecosystem properties. Non-native, invasive species have been identified as a major cause of biodiversity loss and the increasing prevalence of invasive species has prompted studies to help understand their impacts and to conserve biodiversity. Studying mechanisms of invasion also gives insight into how communities are structured and assembled. This study examined mechanisms that contribute to macrophyte invasion. First, I reviewed literature concerning mechanisms of macrophyte invasion. Mechanisms identified with this review were then placed within the context of the invasion process and potential taxonomic biases were discussed. Second, a set of classic invasion hypotheses were tested, including biotic resistance, disturbance, and stress, using mixed-effects models on survey data collected from twenty-nine lakes across the United States. Finally, using the same survey data, I performed an observational test of Darwin’s Naturalization Hypothesis at a small (point) and large (lake) scale for two highly invasive macrophytes, Potamogeton crispus and Myriophyllum spicatum. Results of the first study indicated that many invasion mechanisms have been tested with fully aquatic macrophytes with varied levels of support. In addition, there is likely a taxonomic bias depending on geographic location of the invaded area. The second study indicated that biotic interaction, disturbance, and stress interact, often in non-linear ways to influence probability of an invasive species occurring at a location. However, models containing these variables explained a relatively low percentage of variation in probabilities. Finally, there was no support for Darwin’s naturalization hypothesis at either a point or lake scale. Future research should continue the search for mechanisms that allow introduced species to establish. It is likely that general principles do not exist, at least among comparisons across ecosystem types. However, ecologists should continue to search for general patterns within definable ecosystem units to increase understanding about factors contributing to invasibility.

Biological invasions are a significant area of research due to perceived negative environmental and economic impacts. The study of biological invasions has identified three broad components involved in successful invasions: propagule pressure, abiotic and biotic conditions. Propagule pressure is the product of introduced propagules and the frequency of those introductions, and is considered a driver in all stages of invasion. Data to quantify propagule pressure is often unavailable, and therefore, this research approached genetic information to produce estimates of propagule pressure in the successful invasion of cogongrass (Imperata cylindrica) in the United States. The following research utilized molecular methodologies to estimate genetic diversity and to infer historical introductions. Population genetic analyses were conducted for the purpose of estimating extant population-level genetic diversity at multiple-scales to first address documented cogongrass introduction(s) into this country, followed by an exploration of substantial range expansion into seven states across the region. Lastly, this research explicitly seeks evidence to support interspecific hybridization between cogongrass and a co-occurring congeneric (Imperata brasiliensis) having occurred. Aggressive range expansion by cogongrass has been attributed to such a hybridization event, but has not yet been specifically tested. Historical accounts of foreign introduction of cogongrass propagules identify two separate introductions of distinct source material made into Mississippi and Alabama. Localized population genetic analysis of these two states found substantial genetic variability within and among cogongrass populations, and supported a two-introduction scenario of distinct genetic source propagules establishing and subsequently intermixing. Enlarging the geographic scale of study incorporated five additional U.S. states currently experiencing and managing cogongrass invasion. Considerable genetic variability was found within and among the seven states surveyed. It was found that range expansion was unequal across the range, and that the most distant states tested were not genetically isolated from source populations, suggesting a possible anthropogenic role. Focusing solely on Florida where two congeners overlap ranges, morphology, and ecology, this population genetic analysis failed to detect significant evidence to support interspecific hybridization. Collectively, these investigations explored genome-level dynamics during invasion by a noteworthy invasive grass in an effort to better understand the process of biological invasions.

No description available.

161 miospore species and 122 microplankton species are described from 106 samples in six sections of the Lower Colorado Group and its equivalents in central and west-central Alberta. Two miospore species, and two genera and twenty-five species of microplankton, are newly described. The miospore and microplankton assemblages are used as a basis for division of the Lower Colorado Group into several time-stratigraphic units. The age of the Group is determined as Upper Albian (latest Lower Cretaceous). A method for defining recurrent microplankton species groups is developed and applied to assemblages from two of the sections. The distribution of the recurrent groups formulated is shown to be correlated in part with palynological, lithological and other data. Application and extension of the results and conclusions of this investigation should prove useful in understanding many aspects of western Canadian Cretaceous stratigraphy and in the investigation of microplankton occurrence patterns from other deposits. / Thesis / Doctor of Philosophy (PhD)

ABSTRACT GENETIC VARIABILITY IN HYDRASTIS CANADENSIS L. USING RAPD ANALYSIS FEBRUARY 2009 KERRY J. KELLEY, B.A. MOUNT HOLYOKE COLLEGE M.A. UNIVERSITY OF MASSACHUSETTS AMHERST Directed by: Professor Lyle Craker Hydrastis canadensis L. (goldenseal) is an endangered perennial wildflower species native to eastern North America. In this study, several populations of goldenseal, (both cultivated and wild type) were analyzed for genetic variability. The samples were collected from plant populations in North Carolina, Ohio, Pennsylvania and West Virginia and preserved using silica gel during collection. Random amplified polymorphic DNA (RAPD) analysis technique was used to generate DNA profiles from individual plants and to estimate genetic variability between groups (cultivated and wild type), among populations within groups and within populations using analysis of molecular variance (AMOVA) and a UPGMA clustering phenogram. Our results demonstrate that the bulk of genetic diversity may be within and among populations, but not between groups. This indicates the need for preservation and conservation efforts at the population level. The next step would be to study goldenseal populations more in depth for underlying causes of the genetic variability observed in this study. Further study of genetic variability with different molecular markers may be needed to clarify the level of diversity for the species at the group level. Increased knowledge of genetic variability and the identification of accessions of goldenseal would prove useful for reintroduction and cultivation strategies.

The American chestnut tree (Castanea dentata) is a deciduous tree that largely exists in the eastern United States along the Appalachian Mountain range. Approximately 100 years ago, a fungal pathogen (Cryphonectria parasitica) decimated chestnut populations, resulting in the loss of billions of trees. Disease-resistant American chestnut populations have been developed, but the introgression of wild adaptive diversity into these breeding populations will be necessary to develop locally adapted and disease resistant chestnut trees for reintroduction. In this dissertation, I presented our findings which addressed previous gaps in knowledge regarding the population genomics of wild and backcross American chestnut populations. I 1) estimated the genomic diversity, population structure, and demographic history of remnant wild American chestnut populations; 2) revealed the genomic basis of local climate adaptation in American chestnut, developed a novel method to make tree sampling estimates for germplasm conservation, and defined unique seed zones for American chestnut based on climate and genotype, and 3) determined the amount of wild adaptive diversity captured by the backcross breeding program and made recommendations for their replanting region. These results will inform the development of a breeding plan for the introgression of adaptive diversity into backcross and transgenic chestnut populations. / Doctor of Philosophy / The American chestnut tree (Castanea dentata) is a deciduous tree that largely exists in the eastern United States along the Appalachian Mountain range. Approximately 100 years, a fungal disease (Cryphonectria parasitica) decimated chestnut populations, resulting in the loss of billions of trees. The American Chestnut Foundation developed disease-resistant American chestnut backcross trees by breeding American chestnut trees with Chinese chestnut trees (Castanea mollissima). These trees will need additional breeding with wild American chestnut trees so that their offspring will have both the disease-resistant traits and the adaptations to the local environment where they will be replanted. This is important, because trees that are both disease-resistant and locally adapted will be most likely to survive and thrive in their replanting location. However, a comprehensive evaluation of the genomic basis for local adaptation in American chestnut populations is lacking. In this dissertation, I presented our findings which addressed previous gaps in knowledge regarding the population genomics of wild and backcross American chestnut populations. I 1) estimated the genomic diversity, number of unique populations, and population size changes over time in wild American chestnut; 2) revealed the genes related to local adaptation in American chestnut, developed a novel method to make tree sampling estimates for conserving wild American chestnut diversity, and defined unique seed zones (areas within the species range that have unique adaptations to environment) for American chestnut based on climate (ie, precipitation and temperature values) and genotype (DNA), and 3) determined the amount of wild genomic diversity related to local adaption captured by the backcross breeding program and made recommendations for their replanting region. These results will inform the development of a breeding plan of wild American chestnut with backcross and transgenic chestnut populations to create locally adapted and disease-resistant chestnut populations for reintroduction.

京都大学 / 新制・課程博士 / 博士(理学) / 甲第24456号 / 理博第4955号 / 新制||理||1707(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)准教授 渡辺 勝敏, 教授 曽田 貞滋, 教授 中務 真人 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

京都大学 / 新制・課程博士 / 博士(理学) / 甲第24457号 / 理博第4956号 / 新制||理||1707(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)准教授 中野 隆文, 教授 本川 雅治, 教授 森 哲 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

京都大学 / 新制・課程博士 / 博士(理学) / 甲第24459号 / 理博第4958号 / 新制||理||1708(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)教授 本川 雅治, 准教授 中野 隆文, 教授 中務 真人 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM