Understanding the processes responsible for gradients in biodiversity is a central goal of ecological research. In order to elucidate the processes responsible for community assembly and structure, it is useful to adopt a functional trait approach to community ecology. This is because species names provide little information regarding how constituent species interact. In addition, assembly rules based on species names are likely to become intractably complex with increasing species richness but rules based on traits can provide simple, broadly applicable. In turn, generality is gained by emphasizing functional traits. Here I first build from a previously published model that merged metabolic theory with a model of community evolution and assembly to derive a general assembly rule based on a continuous functional trait and compare this rule with a broad suite of empirical data (Chapter 1). However, linking metabolism to macroevolutionary rates and patterns has thus far been limited to non-ecological, static models. These models are not inconsistent with empirical data, but are relatively limited in their predictive ability (Chapter 2). I thus next develop a fully dynamic `metabolic theory of biodiversity' (MTB) that explicitly implements the qualitative framework proposed in Allen et al. (2007). With this model I examine the influence of temperature dependent mutation rate on speciation rate, extinction rate and species richness (Chapter 2). The model predicts a variable influence of temperature, but the processes responsible for this variation are not immediately clear. I subsequently conduct a detailed analysis elucidating the key processes that allow/constrain a strong influence of temperature dependent mutation rate on species richness (Chapter 3). In addition to mutation rate, temperature-dependent metabolism can influence ecological (feeding and mortality) and ecosystem (e.g. decomposition and in turn nutrient supply) rates. As such, I extend the model developed in chapters 1-3 to incorporate these additional temperature dependencies and derive predictions for the influence of temperature over species richness (Chapter 4).

Mechanisms of cell death extend beyond the simple apoptosis/necrosis relationship to include regulated modes of cell death that do not readily fit either of the classic descriptors. One such mechanism of cell death involves poly(ADP-ribose)polymerase-1 (PARP-1)-mediated cell death. 2,3,5-Tris(Glutathion-S-yl)-hydroquinone (TGHQ), a reactive oxygen species (ROS) generating nephrotoxic and nephrocarcinogenic metabolite of hydroquinone, causes necrotic renal cell death, the basis for which is unclear. We therefore investigated TGHQ-mediated cell death in human renal proximal tubule epithelial HK-2 cells. TGHQ induced ROS generation, DNA strand breaks, hyperactivation of PARP-1, rapid depletion of nicotinamide adenine dinucleotide (NAD), elevations in intracellular Ca2+ concentrations, loss of mitochondrial membrane potential, and subsequent necrotic cell death. Interestingly, PARP-1 hyperactivation was not accompanied by the translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus, a process usually associated with PARP-dependent cell death. Inhibition of PARP-1 with PJ34 blocked TGHQ-mediated accumulation of poly(ADP-ribose) polymers, NAD consumption, and the consequent necrotic cell death. However, HK-2 cell death was only delayed by PJ34, and cell death remained necrotic in nature. In contrast, chelation of intracellular Ca2+ with BAPTA-AM completely abrogated TGHQ-induced necrotic cell death. Ca2+ chelation not only prevented the collapse in the mitochondrial potential but also attenuated PARP-1 hyperactivation. Conversely, inhibition of PARP-1 modulated TGHQ-mediated changes in Ca2+ homeostasis. Moreover, TGHQ caused a sequential oxidation of peroxiredoxin III (PrxIII), a protein considered the primary antioxidant defense within mitochondria. Thus, TGHQ induced two acidic shifts in PrxIII, with both pI shifted spots representing oxidized forms of PrxIII. Transient expression of a dominant negative version of PrxIII resulted in a significant increase in TGHQ-induced cytotoxicity, whereas overexpression of wild-type PrxIII significantly attenuated cytotoxicity. Our studies provide new insights into PARP-1-mediated necrotic cell death. Changes in intracellular Ca2+ concentrations appear to couple PARP-1-hyperactivation to subsequent cell death, but in the absence of AIF release from mitochondria. NAD depletion, mitochondrial membrane depolarization, Ca2+-mediated calpain activation, and PrxIII oxidation, all contribute to TGHQ-driven ROS-mediated necrotic cell death.

The goal of this inductive study was to identify factors that facilitate and inhibit binational collaboration in the recovery of endangered species in the northern Mexico borderlands, focusing on the Mexican wolf (Canis lupus baileyi). A conceptual model was developed using qualitative techniques, providing the basis for design of a mail survey. The target population included participants with experience in recovery efforts for over a dozen species at risk in the region. Long interviews were recorded with 44 participants from Mexico and the United States. Thematic hierarchical analysis was used to develop a conceptual model of how interviewees talked about factors influencing binational collaboration. Issues were classified in five thematic clusters: project, organization, people, resources, culture/history. The survey was used to conduct a needs assessment, measuring respondents' attitudes about the relative priority of issues identified in the conceptual model. High priority needs were identified from each thematic cluster: (a) equitable participation in project design and implementation, (b) continuity of personnel, (c) coordination of federal, state and local efforts, (d) increased funding, managed with accountability, and (e) exchange visits to facilitate understanding of diverse perspectives. Responses to almost half the survey items indicated accord among the sample of respondents, providing a basis for shared common ground. The nature of discord was within the range of "manageable", with no clear polarization of attitudes measured. This exploratory data analysis suggested that the structure of the conceptual model developed from the Mexican wolf case study was generally a valid basis for future deductive analysis and reflection by practitioners. For 82% of 22 statements of need, priorities of participants in the Mexican wolf recovery efforts did not differ significantly from other respondents. Nationality (of respondents) significantly affected priority rankings for only 18% of the need statements. Significant effects of five demographic variables indicated that interactive effects should be examined in future multivariate analyses to determine how respondents' attitudes on issues related to priority rankings. Recommendations were provided for a more efficient and effective approach to collaborative problem-solving, engaging reflective practitioners from the private and public sectors in principled negotiation processes to better understand diverse perspectives.

Environmental heterogeneity has often been suggested as a general explanation for patterns of diversity at scales ranging from individuals within populations to communities within landscapes. I evaluate this proposition using laboratory experiments with two microbial species, the unicellular chlorophyte Chlamydomonas reinhardtii and the common bacterium Pseudomonas fluorescens. These experiments contrast the fate of diversity following selection in heterogeneous and homogeneous environments. Specifically, I show that (1) an individual's breadth of adaptation evolves to match the amount of environmental variation, specialists evolving in environments that remain constant through time and generalists evolving in environments that vary through time irrespective of the scale at which environmental variation occurs relative to the lifetime of an individual; (2) the maintenance of diversity in a spatially heterogeneous environment is context-dependent, diversity being more readily maintained when environmental conditions are very different and genotypes are widely divergent; (3) selection in heterogeneous environments represents a plausible mechanism for two well-known patterns of diversity at large spatial scales, namely that between species diversity and both productivity and disturbance. This thesis thus demonstrates that environmental heterogeneity is a plausible, and perhaps very general, factor responsible for the diversity of natural communities.

This thesis attempts to uncover the reasons why Canada, unlike the United States and Australia, does not have stand alone federal endangered species legislation. In particular, I will focus upon the history of Bill C-65, the proposed federal endangered species statute which died on the Order Table in 1997. Using the "policy regime" framework developed by George Hoberg, I examine the ideas, actors, and institutions that have combined within a given set of background conditions to produce this distinctive lack of a policy outcome, assessing the relative causal importance of each variable in terms Bill C-65's failure. Using Peter Haas' epistemic community approach, the causal knowledge of conservation scientists' regarding habitat loss is found not to have influenced the policy substance of Bill C-65. However, it is argued that scientists did play an important role in the legislative failure insofar as they joined forces with environmentalists to discredit the weak scope and substance of the bill. These pro-environment actors, however, were matched throughout the interest group competition by the parallel forces of industry and private landowner groups, who criticized Bill C-65 as a litigious, punitive and "American" style of legislation. The provinces, for their part, sided with the landowners and industry groups, arguing that the federal government had overstepped its wildlife management jurisdiction. Given a context of low public concern for environmental issues, and the institutional trend towards regulatory decentralization, the federal government had very few incentives to introduce a strong bill. However, the provinces, landowners, and industry groups, all felt it was too strong, while environmentalists and scientists felt just the opposite. Bill C-65's failure, therefore, was the result of the federal government's inability to satisfy anyone on this issue. Determining who "won" this first endangered species battle, however, is quite difficult without knowing whether Cabinet felt the bill was too strong or too weak, and without knowing what the next legislative proposal will entail. In conclusion, it is found that all three regime components of ideas, actors, and institutions were equally important factors in bringing about the failure of Bill C-65, and the current policy delay that continues to this day.

Kaunas sea contains more than 500 mln. m3 of Nemunas water. The Kaunas' sea Regional park was established at 1992. The purpose - to retain ecosystem, landscape, and inheritance of culture. Water molluscs - invertebrate organisms. They are very important in such ecosystem. Outspread of water molluscs is interconnected with climatic and edafical factors and is not influenced by such factors as competition.

Two projects were undertaken to study the non-energy conserving alternative pathway present in the plant mitochondrial ETC. In the first project, a tobacco AOX2 gene was cloned and characterized. AOX2 showed tissue specificity in expression and could not be induced by common stresses. In the second project I carried out a physiological characterization of transgenic tobacco plants with increased or decreased expression of AOX1 subjected to cold stress. Under non-stress condition, a strong inverse relationship between levels of AOX1 and levels of oxidative damage was observed, while after cold treatment AOX1 transgenic lines and WT showed more complicated and differential responses in aspects of oxidative damage and the capacity of antioxidant system. I also discovered that the pool sizes of monosaccharides after temperature shift were proportional to AOX1 levels. These results indicated that AOX1 might have crucial but complex impacts on ROS balance and carbon metabolism during cold stress.

Two projects were undertaken to study the non-energy conserving alternative pathway present in the plant mitochondrial ETC. In the first project, a tobacco AOX2 gene was cloned and characterized. AOX2 showed tissue specificity in expression and could not be induced by common stresses. In the second project I carried out a physiological characterization of transgenic tobacco plants with increased or decreased expression of AOX1 subjected to cold stress. Under non-stress condition, a strong inverse relationship between levels of AOX1 and levels of oxidative damage was observed, while after cold treatment AOX1 transgenic lines and WT showed more complicated and differential responses in aspects of oxidative damage and the capacity of antioxidant system. I also discovered that the pool sizes of monosaccharides after temperature shift were proportional to AOX1 levels. These results indicated that AOX1 might have crucial but complex impacts on ROS balance and carbon metabolism during cold stress.

Detrital subsidy from highly productive kelp beds and forests to adjacent habitats represents a major form of connectivity between coastal ecosystems that regulates regional patterns of community organization and production. In this thesis, I investigated environmental and biological factors that influence the rate of detrital production in Nova Scotian kelp beds, with emphasis on the role of invasive species in altering these dynamics. The rate of blade erosion of the dominant kelp species (Saccharina latissima and Laminaria digitata) increased significantly with the level of encrustation by the invasive bryozoan Membranipora membranacea and the extent of grazing damage by the native snail Lacuna vincta, and by increased water temperature and site exposure. The rate of detrital production (as dry mass) ranged from 0.5 to 1.71 kg m-2 y-1 across 5 sites, and increased linearly with kelp bed biomass. Spatial variation in the total level of grazing damage on kelp blades by L. vincta was explained in part by a negative relationship with site exposure, and the distribution of grazing along blades was regulated by kelp growth rate and the associated production of grazing-deterrent polyphenolics. Grazing damage by L. vincta that exceeded 0.5 to 1.0% of blade area caused increased rates of erosion during heavy wave action associated with a passing hurricane. The maximum stress before breakage, toughness, and extensibility of blade tissues decreased with the degree of encrustation by M. membranacea or grazing damage by L. vincta, which cause degradation and removal of the outer cell layers of kelp tissues, resulting in stress concentration and breakage at lower force applications than required to break undamaged tissues. The invasive green alga Codium fragile and S. latissima differed with respect to nutritional quality and changes in biochemical composition that occurred over the course of degradation on a sandy bottom adjacent to a kelp bed. Macrofaunal communities colonizing detrital deposits responded to these differences. These findings demonstrate that invasive species can alter the quantity and quality of detritus produced from subtidal kelp beds, and that their community-level effects can extend well beyond the invaded habitats via the export of detritus.

Protecting existing habitat for species-at-risk is an important conservation measure; however, many populations occupy highly fragmented habitat patches to the extent that population persistence is unlikely without the creation of new habitat patches. This research examines the potential for clusters of rehabilitated aggregate extraction sites to be used as reintroduction sites for species-at-risk. Using a method combining GIS and metapopulation modeling, I evaluated the success of establishing metapopulations of the Five-Lined Skink, Eumeces fasciatus, in 137 clusters of aggregate extraction sites using three hypothetical recovery scenarios. Patch abundance and patch clustering had a significant negative effect on metapopulation extinction risk. Increasing the quality of a single patch relative to other patches of suitable quality had little effect on extinction risk and patch occupancy. Introducing all individuals into a single patch decreased extinction risk and patch occupancy. Rehabilitated aggregate sites could play a role in the protection of species-at-risk in Ontario.