The mechanisms that allow invasive species to flourish in novel habitats are poorly understood. A. petiolata (Alliaria petiolata) is an invader of North American forest edge and understory communities. A. petiolata’s success may result from high competitive ability due to its suppression of mycorrhizal fungi used by other species. In a greenhouse experiment, I grew 27 target species in the presence and absence of A.petiolata in soil with and without a history of A. petiolata. I found that soil with a history of A. petiolata had depleted plant available nitrogen and reduced mycorrhizal fungal colonization of target species. A. petiolata competition suppressed the growth of target species less in soils with than without A. petiolata history. Furthermore, I found that tall plants with finer roots more effectively suppressed the growth of A. petiolata. My results suggest that soil feedback effects do not improve A. petiolata’s competitive ability and are unlikely to contribute to A. petiolata invasion.

The endemic landbirds of the Seychelles granitic islands have suffered considerable losses due to predation by introduced rats and cats and extensive habitat destruction. With less than 100 individuals, the Critically Endangered Magpie Robin Copsychus sechellarum Newton, faces the greatest risk of extinction. Translocations to three predator-free islands, Aride, Cousin and Cousine, have provided valuable opportunities for gaining insights into the ecology of the species. Of particular interest are links between the Magpie Robin, endemic skinks Mabvya spp., ground-living invertebrates and seabird colonies. Magpie Robin faecal pellet and skink gut content analysis demonstrated a high degree of dietary overlap between the species. A widespread exotic cockroach Pycnoscelus indicus was the favourite prey item for each species. However, behavioural observations and a dietary choice experiment indicated that there is no significant competition for food during the main seabird breeding season. Invertebrate sampling on Cousine identified 52 species which were available in all habitat types currently in use or those considered suitable for the Magpie Robin. Seabird and skink density counts on Cousine demonstrated the considerable magnitude of vertebrate organic food also available. Invertebrate sampling results on Cousin and Cousine were used to determine territory quality and the carrying capacity of each island for the Magpie Robin. While most seabirds are not breeding, skink survival depends on invertebrate abundance. M. wrightii weight declined throughout this period but that of M. sechellensis remained fairly stable. The data were insufficient to conclude that inter-specific competition for food exists between M. sechellensis and the Magpie Robin during this period. Further expansion of the Magpie Robin population depends on eradicating mammalian predators from other islands and maximising the potential carrying capacities of those already supporting the species. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 1998.

Submitted by Claudia Rocha (claudia.rocha@udesc.br) on 2017-12-11T12:58:02Z No. of bitstreams: 1 PGEF16MA068.pdf: 201689 bytes, checksum: e9497705cc6674fb160253fc595a4dbb (MD5) / Made available in DSpace on 2017-12-11T12:58:02Z (GMT). No. of bitstreams: 1 PGEF16MA068.pdf: 201689 bytes, checksum: e9497705cc6674fb160253fc595a4dbb (MD5) Previous issue date: 2016-07-08 / FAPESC / This dissertation aimed to understand the process of biological invasion by Ligustrum spp, in a peri-urban forest remnant. For this, a temporal analysis of the organization of floristic-structural and diversity patterns of the tree component was conducted in an Araucaria Forest fragment, in the municipality of Lages, SC, in 2012, 2014 and 2015. In 2012, five transects were allocated, each on divided into 20 x 20 m plots, covering a gradient of 0-100m from edge to forest interior, with each plot presenting sub-plots to evaluate the regenerative component. For the adult component all trees with cbh (circumference at breast height) greater than or equal to 15,7 cm were measured (cbh). For the regenerative component, the inclusion level varied according to size class: Class 1, plants with height between 10 cm and 1 m, in sub-plots of 1 x 5 m; Class 2, plants with height between 1 and 3 m, in sub-plots of 1 x 10 m; and Class 3, plants with a height greater than 3 m and cbh smaller than 15,7 cm, in sub-plots of 1 x 20 m. In 2014 (for regeneratives) and 2015 (for adults), dead and recruit individuals were counted, adult survivors were re-measured, and the height class of regenerative individuals were determined. The rates of mortality, recruitment, basal area gain, basal area loss, turnover and size class changes of regenerative component were determined. The dynamics data of regenerative components were analyzed through Principal Component Analysis (PCA) and contingency table; for the adult component, plots were classified in contaminated (C) and not contaminated (NC) by invasive species, with the demographic rates between these two conditions compared. The c-score index was used for the evaluation of species coexistence pattern in contamined and non-contamined plots. We conclude that the studied forest is under a process of post-disturbance recovery, with Ligustrum spp. showing a high population increment in the area. Whereas in the adult component the results suggest the invase species as an opportunist, in the regenerative component it is inferred that the species occurs as an important competitive in relation to native species / A presente dissertação teve como principal objetivo compreender o processo de invasão biológica por Ligustrum spp. em um remanescente florestal periurbano. Para isto, foi realizada uma análise temporal da organização florística-estrutural e dos padrões de diversidade do componente arbóreo em um fragmento de Floresta Ombrófila Mista localizado em Lages, SC, nos anos de 2012, 2014 e 2015. Em 2012, foram alocadas cinco transecções, sendo cada transecção dividida em parcelas, 20 x 20 m, cobrindo um gradiente de 0-100m entre a borda e o interior da floresta, sendo que em cada parcela constavam sub-parcelas para avaliação do componente regenerativo. Para o componente adulto, foram avaliados todos os indivíduos arbóreos com CAP (circunferência medida a altura do peito) maior ou igual a 15,7 cm. Para o componente regenerante, o nível de inclusão variou em função da classe de tamanho: Classe 1, plantas com altura entre 10 cm e 1 m, em sub-parcelas de 1 x 5 m; Classe 2, plantas com altura entre 1 e 3 m, em sub-parcelas de 1 x 10 m; e Classe 3, plantas com altura maior que 3 m e CAP menor que 15,7 cm, em sub-parcelas de 1 x 20 m. Em 2014 (regenerantes) e 2015 (adultos), esses indivíduos foram reavaliados, contabilizando a mortalidade e recrutamento, remedindo os CAP`s dos adultos sobreviventes e determinando a classe de altura dos regenerantes. Foram calculadas as taxas de mortalidade, recrutamento, ganho e perda em área basal, rotatividade e mudanças de classes de altura dos regenerantes. Os dados para componente regenerante foram analisados por meio de uma Análise de Componentes Principais (PCA) e tabela de contingência; para o componente adulto, as parcelas foram classificadas em contaminadas (C) e não contaminadas (NC) pela espécie invasora, sendo as taxas de demográficas comparadas entre essas duas condições. Para avaliar o padrão de coexistência das espécies nas parcelas contaminadas e não-contaminadas, foi utilizado o índice de c-score. Conclui-se que a floresta estudada encontra-se em fase de recuperação pós-distúrbio, com Ligustrum spp. demonstrando elevado incremento populacional na área. Enquanto no componente adulto os resultados observados sugerem um comportamento oportunista da espécie invasora, no regenerante infere-se que a espécie ocorre com uma importante competidora com as espécies nativas

ABSTRACT Assessing the impact of herbivory on plant growth and reproduction is important to predict the success of biocontrol of invasive plants. Leaf area production is most important, as photosynthesis provides the foundation for all plant growth and fitness. High levels of defoliation generally reduce the productivity of plants. However, leaf area production fluctuates during the season and compensational growth may occur, which both complicate accurate estimations of defoliation impacts. Under field conditions the interaction with neighbouring species and the availability of soil nutrients need to be assessed in order to gauge long term effects of weed invasions on natural environments. In this thesis I have investigated seasonal leaf area dynamics in Buddleia davidii following repeated artificial defoliation, to quantify compensational leaf production and to understand the regulatory mechanisms involved. The impact of defoliation on photosynthesis, seed production, germination and nitrogen translocation patterns were analysed. Finally, possible facilitation between B. davidii and a native nitrogen fixer, Coriaria arborea, and the impact of B. davidii on soil nutrient availability were investigated. In defoliated B. davidii, increased node production (34%), leaf size (35%) and leaf longevity (12%) resulted in 52% greater total emergent leaf area in the short term. However, with time and diminishing tissue resources the compensation declined. No upregulation of photosynthesis was observed in pre-existing leaves. Compensational leaf area production occurred at the expense of reproduction but the germination capacity of individual seeds was unaffected. In B. davidii, nitrogen reserves are stored in old leaves. Thus, the defoliation-induced decline in tissue reserves led to changes in the remobilisation pattern and increased the importance of soil uptake but biomass production especially that of roots had declined significantly (39%). Slight facilitation effects from the neighbouring nitrogen fixer and VA-mycorrhizae were observed on B. davidii in the field, while its impact on soil chemistry during spring was negligible. Defoliation of B. davidii resulted in priority allocation of resources to compensational leaf growth and a concomitant reduction in flower and seed production. The compensational leaf production greatly increased the demand for nitrogen, while continued leaf removal decreased the pool of stored nitrogen. This led to changes in nitrogen remobilisation and an increased importance of root uptake. However, the significant decline in root growth will likely impair adequate nutrient uptake from the soil, which is especially important where B. davidii invades nutrient poor habitats and will increase the success of biocontrol of the species. While mycorrhizae increase nutrient accessibility for B. davidii, it is likely that the additional stress of defoliation will negate the small facilitative effects from nitrogen-fixing species like C. arborea. This research provides new insights into the mechanisms regulating leaf area dynamics at the shoot level and systemic physiological responses to defoliation in plants, such as nitrogen translocation. The compensation in leaf area production was considerable but only transitory and thus, the opportunity to alleviate effects of leaf loss though adjustment of light capture limited. However, to ascertain that photosynthesis at whole plant level does not increase after defoliation, more detailed measurements especially on new grown leaves are necessary. In general, defoliation had greatly reduced plant growth and performance so that an optimistic outlook for controlling this species can be given. Conclusions about the wider impacts of B. davidii on soil chemistry and community function will require further research.

ABSTRACT Assessing the impact of herbivory on plant growth and reproduction is important to predict the success of biocontrol of invasive plants. Leaf area production is most important, as photosynthesis provides the foundation for all plant growth and fitness. High levels of defoliation generally reduce the productivity of plants. However, leaf area production fluctuates during the season and compensational growth may occur, which both complicate accurate estimations of defoliation impacts. Under field conditions the interaction with neighbouring species and the availability of soil nutrients need to be assessed in order to gauge long term effects of weed invasions on natural environments. In this thesis I have investigated seasonal leaf area dynamics in Buddleia davidii following repeated artificial defoliation, to quantify compensational leaf production and to understand the regulatory mechanisms involved. The impact of defoliation on photosynthesis, seed production, germination and nitrogen translocation patterns were analysed. Finally, possible facilitation between B. davidii and a native nitrogen fixer, Coriaria arborea, and the impact of B. davidii on soil nutrient availability were investigated. In defoliated B. davidii, increased node production (34%), leaf size (35%) and leaf longevity (12%) resulted in 52% greater total emergent leaf area in the short term. However, with time and diminishing tissue resources the compensation declined. No upregulation of photosynthesis was observed in pre-existing leaves. Compensational leaf area production occurred at the expense of reproduction but the germination capacity of individual seeds was unaffected. In B. davidii, nitrogen reserves are stored in old leaves. Thus, the defoliation-induced decline in tissue reserves led to changes in the remobilisation pattern and increased the importance of soil uptake but biomass production especially that of roots had declined significantly (39%). Slight facilitation effects from the neighbouring nitrogen fixer and VA-mycorrhizae were observed on B. davidii in the field, while its impact on soil chemistry during spring was negligible. Defoliation of B. davidii resulted in priority allocation of resources to compensational leaf growth and a concomitant reduction in flower and seed production. The compensational leaf production greatly increased the demand for nitrogen, while continued leaf removal decreased the pool of stored nitrogen. This led to changes in nitrogen remobilisation and an increased importance of root uptake. However, the significant decline in root growth will likely impair adequate nutrient uptake from the soil, which is especially important where B. davidii invades nutrient poor habitats and will increase the success of biocontrol of the species. While mycorrhizae increase nutrient accessibility for B. davidii, it is likely that the additional stress of defoliation will negate the small facilitative effects from nitrogen-fixing species like C. arborea. This research provides new insights into the mechanisms regulating leaf area dynamics at the shoot level and systemic physiological responses to defoliation in plants, such as nitrogen translocation. The compensation in leaf area production was considerable but only transitory and thus, the opportunity to alleviate effects of leaf loss though adjustment of light capture limited. However, to ascertain that photosynthesis at whole plant level does not increase after defoliation, more detailed measurements especially on new grown leaves are necessary. In general, defoliation had greatly reduced plant growth and performance so that an optimistic outlook for controlling this species can be given. Conclusions about the wider impacts of B. davidii on soil chemistry and community function will require further research.

The Swedish Transport Administration works continuously with biological diversity. The project, “Species-rich road verges”, begun in 1995. A species-rich road verge is a road verge area that houses objects worthy of protection. It is common for a species-rich road verge to contain meadow species but on the other hand, road verges also function as new habitat for invasive species. Invasive species often tend to show faster growth and higher reproductive potential than non-invasive species and are considered to homogenize ecosystems because many native species often have difficulty competing with them. The invasive Garden lupine (Lupinus polyphyllus) can be particularly problematic at species-rich road verges, where it competes with the native flora. The purpose of the study was to investigate whether the presence of L. polyphyllus in species-rich road verges decreases plant diversity and species richness, if it changes the species composition and if it affects the vegetation height. The study also wanted to investigate if the presence of L. polyphyllus in species-rich road verges presence affects the thickness of the litter, the pH-value in the soil and the ecological indicator values. This study was conducted at 12 species rich road verges in Värmland and Örebro counties in Sweden, where vascular plants in a box of 1 m2 were inventoried along the road verges in pairs, each pair containing a plot where L. polyphyllus was present (“lupine plot”) and a plot where L. polyphyllus was absent (“control plot”). At each species-rich road verge as many pairs as the length of the road verge allowed was inventoried. Vegetation height, litter thickness and pH- value were also measured and ecological indicator values was calculated for each plot. The results suggest that the presence of L. polyphyllus decreases species richness and diversity and changes the species composition in species-rich road verges so that it becomes homogenized. Vegetation height was affected when L. polyphyllus was present, even when the species itself was not taken into the calculation. The results of this study indicate that it is important to come up with effective strategies to control and stop the spread of the invasive L. polyphyllus in species-rich road verges. / Trafikverket arbetar kontinuerligt med biologisk mångfald. Projektet Artrika vägkanter började 1995. En artrik vägkant är ett vägkantsområde som hyser skyddsvärda arter. Det är vanligt att en artrik vägkant innehåller ängsarter. Vägkanter fungerar också som en ny livsmiljö för invasiva arter. Invasiva arter tenderar ofta att visa snabbare tillväxt och högre reproduktionspotential än icke-invasiva arter och anses homogenisera ekosystem då de konkurrerar ut många inhemska arter. Den invasiva blomsterlupinen (Lupinus polyphyllus) kan vara särskilt problematisk vid artrika vägkanter där den konkurrerar med den inhemska floran. Syftet med studien var att undersöka om förekomsten av L. polyphyllus i artrika vägkanter minskar växternas artrikedom och diversitet, om artsamman- sättningen förändras, om vegetationshöjden påverkas samt om abiotiska faktorer påverkas. Studien genomfördes vid 12 artrika vägkanter i Värmland och Örebro län i Sverige, där kärlväxter i en ruta på 1 m2 inventerades längs de artrika vägkanterna i par, med en lupinruta kontra en kontrollruta. Vid varje artrik vägkant inventerades så många par som vägkantens längd tillät. Vegetationshöjd, förnans tjocklek och pH-värde mättes också vid varje ruta och ekologiska indikator värden beräknades för varje ruta. Resultaten tyder på att blomsterlupinen minskar artrikedom och mångfald och att artsammansättningen i lupin rutor förändras jämfört med kontroll rutor och blir mer homogen. Vegetationshöjden påverkades av L. polyphyllus och var signifikant högre i lupinrutor jämfört med kontrollrutor även då arten L. polyphyllus inte räknades med i analysen. Resultaten av denna studie indikerar att det är viktigt att arbeta fram effektiva strategier för att kontrollera och stoppa spridningen av den invasiva L. polyphyllus i artrika vägkanter.

In light of increasing harmful algal blooms and the need to protect human health and aquatic resources, proactive management approaches merit further study. For this purpose I conducted field samplings to characterize plankton community composition and laboratory experiments to test some approaches to new management schemes in the lower Rincon Delta. On site measurements and microscopic analysis showed that environmental parameters and plankton community composition varied considerably among sampling stations and sampling dates. A recent modeling study suggested that manipulation of freshwater inflow to estuaries might prevent phytoplankton blooms and enhance secondary productivity. To test this theory I conducted three semi-continuous design and flow-through incubation design experiments using natural plankton assemblages. I investigated the effect of two different pulsing regimes of inflow and nutrient loading on zooplankton densities, and phytoplankton biomass and diversity. Despite differences in zooplankton structure and phytoplankton community composition between the two experiment designs, the results confirmed that pulsed inflows might alter plankton dynamics. My findings showed that 3-day pulse treatments consistently supported greater zooplankton densities and higher phytoplankton species diversity when compared to 1-day pulse treatments. In addition, accumulation of phytoplankton biovolume remained low during 3-day pulse treatments. Differences in zooplankton performance between 3-day pulse and 1-day pulse inflow treatments were likely due to the ability of phytoplankton to uptake and store greater amounts of nutrients under conditions of 3-day pulse inflow. This resulted in food of higher quality for zooplankton, and might have supported greater zooplankton population growth rates. Additionally, in an attempt to understand the mechanisms leading to high biodiversity in aquatic ecosystems, I built a resource-storage model and studied the effects of resource-storage on competition of multiple phytoplankton species on multiple abiotic resources. I compared this model with a well-established multi-species competition model. My results showed that for certain species combinations a resource-storage-based model can generate dissimilar outcomes when compared to a model without resource-storage.

In light of increasing harmful algal blooms and the need to protect human health and aquatic resources, proactive management approaches merit further study. For this purpose I conducted field samplings to characterize plankton community composition and laboratory experiments to test some approaches to new management schemes in the lower Rincon Delta. On site measurements and microscopic analysis showed that environmental parameters and plankton community composition varied considerably among sampling stations and sampling dates. A recent modeling study suggested that manipulation of freshwater inflow to estuaries might prevent phytoplankton blooms and enhance secondary productivity. To test this theory I conducted three semi-continuous design and flow-through incubation design experiments using natural plankton assemblages. I investigated the effect of two different pulsing regimes of inflow and nutrient loading on zooplankton densities, and phytoplankton biomass and diversity. Despite differences in zooplankton structure and phytoplankton community composition between the two experiment designs, the results confirmed that pulsed inflows might alter plankton dynamics. My findings showed that 3-day pulse treatments consistently supported greater zooplankton densities and higher phytoplankton species diversity when compared to 1-day pulse treatments. In addition, accumulation of phytoplankton biovolume remained low during 3-day pulse treatments. Differences in zooplankton performance between 3-day pulse and 1-day pulse inflow treatments were likely due to the ability of phytoplankton to uptake and store greater amounts of nutrients under conditions of 3-day pulse inflow. This resulted in food of higher quality for zooplankton, and might have supported greater zooplankton population growth rates. Additionally, in an attempt to understand the mechanisms leading to high biodiversity in aquatic ecosystems, I built a resource-storage model and studied the effects of resource-storage on competition of multiple phytoplankton species on multiple abiotic resources. I compared this model with a well-established multi-species competition model. My results showed that for certain species combinations a resource-storage-based model can generate dissimilar outcomes when compared to a model without resource-storage.