The first observations of Florida burrowing owls (Athene cunicularia floridana) occurred in the 19th century on historical dry prairie habitat in south central Florida. These early observations documented the ecology of burrowing owls in rural environments. Since then the vast majority of research on this subspecies has been undertaken in suburban and urban environments during the breeding period. The research undertaken on burrowing owls in suburban and urban environments includes determining natal dispersal distance, assessing female fecundity, mate fidelity, territory fidelity, date of juvenile and adult dispersal from breeding habitat, date of clutch initiation, nesting success, density of breeding pairs, causes of mortality, prey preference, and minimum annual survival of fledglings, juveniles, and adults. Very little research has been undertaken on burrowing owls in rural environments. The purpose of this thesis was to elucidate the behavior and ecology of burrowing owls in a rural environment. The topics researched in this thesis include home range in breeding habitat, dispersal distance to post-breeding habitat, location of post-breeding habitat, behavior during the breeding period, diet of rural versus urban owls, and the evaluation of three methods to trap burrowing owls. The results of this thesis indicate that, during the daytime, juvenile burrowing owls utilized habitat very close to the main and satellite burrows during the breeding period. At night juvenile owls foraged in an extensive saw palmetto patch surrounding the breeding habitat. The predominant prey of both rural and urban burrowing owls during the breeding period was insects. Dispersal of juvenile burrowing owls from breeding habitat coincided with the flooding of the breeding habitat during the rainy season.

The full geographic distribution and habitat use of the Florida Burrowing Owl, a state "Species of Special Concern," is not well-understood, particularly in remote, non-urban areas. This thesis aimed to expand and improve knowledge about non-urban burrowing owls. We first compiled databases of historic sighting observations. Fieldwork verified and updated existing breeding observation point records and also yielded new breeding locations. Using a GIS, we characterized observed land use, landcover, relevant soil attributes, projected future land use and managed area status for selected points. We quantified landcover within biologically-determined buffer distances around burrows from our own field-verified records. Using standard resource selection methods, we compared observed and available proportions, calculated selection indices, and determined selection/avoidance for each landcover class. These empirical results were used in combination with expert opinion and literature review to finalize criteria for and map "suitable" landcover. Suitability of relevant soil attributes were also empirically-determined and used to further reduce the overall "suitable" area. The final suitable habitat maps appear to relate well to the overall distribution of known non-urban burrowing owl records and demonstrate that a great deal of potentially-suitable breeding habitat exists throughout Florida's central interior. Improved pasture, the most prevalent landcover class, also appears to be the most strongly selected in this study and may be of high importance to non-urban, breeding burrowing owls. Our results could be useful to wildlife officials managing this species. Recommendations include improving surveys and conservation efforts in non-urban areas and enhancing cooperation with landowners, particularly ranchers, as success on private lands seems critical to the long-term persistence of this species.