The spindle assembly checkpoint (SAC) is an evolutionarily conserved mechanism that delays the initiation of anaphase by inhibiting the Anaphase Promoting Complex (APC) until all kinetochores have achieved bipolar attachment on the mitotic spindle. Mad1-3, Bub1, and Bub3, components of the SAC, are conserved from yeast to humans. These proteins localize to unattached kinetochores, though it is unknown with which kinetochore proteins they interact and how these interactions transduce information about microtubule attachement. Here, purification of the checkpoint proteins from Saccharomyces cerevisiae suggests that Mad1 interacts with the outer kinetochore protein Ndc80 in a SAC, cell cycle, and DNA dependent manner. Ndc80 is thought to mediate attachment of kinetochores to microtubules so the interaction between Mad1 and Ndc80 suggests a mechanism by which cells sense kinetochore-microtubule attachment. The SAC is of special importance in some types of cancer where genetic damage and aneuploidy is correlated with mutated SAC genes. A better understanding of the SAC mechanism will aid in the development of targetted cancer therpeutics.

This semestral work deals with the design and realization of multipurpose milling machine for production of prototype small components made from soft materials and PCBs. Thesis contains design of mechanical construction, individual parts of driving machine and graphic user interface.

The master´s thesis deals with the design of the headstock for the horizontal boring center and design of the milling head. In the first part of the thesis, a search is made in the field of boring machines, main parts of boring machine headstock and milling heads. Based on this theoretical analysis, the input parameters for the calculation of the tension, power and torque characteristics of the headstock are selected. Subsequently, the design of the headstock and milling head is done using a 3D model.

This master’s thesis deals with online vibration diagnostics of the milling machine spindle. It is aimed at the implementation of the complex online vibration diagnostics system to chosen milling machine, the suggestion of the vibration measurement system and the suggestion of the evaluation of changes of the technical condition of this milling machine spindle. The description of the vibration diagnostics, the description of the milling machine spindle construction and function and the suggestions for the practical application of vibration diagnostics are also parts of this thesis.

The thesis contains of draft, calculations, CAD model and drawing documentation of the moltifunctional machining center with horizontal axis of spindle. This thesis is one of the four parts with which connection ensue the machine-tool. In the first part of my work, there is a constructional draft of the headstock which will enable milling, turning and drilling. In the introduction of this part is headstock research which is solving the most important parts of the headstock of machine . Consequently, technical calculations are acoomplished. Based on these calculations, a specific constructional draft of the headstock is crafted. In the next part of my work, I do research in the area of removable paring milling heads. After this part, there are technical calculations and the specific constructional draft. In the entire work, there is emphasis put on simplicity and functionality.

This master´s thesis deals with the design of the headstock of a turning center. The aim of the work is the design solution of turning headstock with output of about 5 kW with the possibility of positioning in the C axis. The introductory part of this thesis is focused on the descripti-on of the basic parameters of the headstock and turning centers on the market. The next part deals with choice of cutting conditions and calculation of spindle parameters. These calculations are verified using the FEM method. Based on the calculations and design requirements, a headstock model with drawing documentation is created.

The subject of this diploma thesis is the design solution of the axial headstock, its gear box and main drive. This headstock is used for heavy duty gantry type machines from the production of TOS Kurim company. The thesis contains technical solution of the headstock, an analysis of the construction of the headstock drive, overview of the possible propulsion units, choice of the best drive variant, control calculations, technical proposal of drive design and economical evaluation of the whole reconstruction.

The goal of this work is to propose a suitable methodology for measuring and evaluating the geometric precision of spindle rotation. Identification and classification of errors while moving spindle. Part of this work should be also a program in LabVIEW that evaluate the errors in the motion of the spindle. Practical functionality of the proposed measurement system should be tested at selected MCV754 QUICK machines, lathes SV 18 and milling machine FNG 32. For processing of the results should be used basic statistical procedures. This thesis includes research in the field of machine tool spindles and different ways of measuring spindle motion deviations from the ideal path. There is also included the effect of sensors to measuring spindle running accuracy and identification of suitable sensors applicable for this application. There is a proposal of measurement of selected machinery, and defines all the components required for measurements that were used during spindle running precision measurement. Part of this work is a basic description of the program developed for evaluating errors in the motion of the spindle.

The aim of this thesis called Design of bar-feeder for CNC lathe is research about workpiece handling and bar feeders. But the most important aim is design of bar feeder by chosen parameters. The first part of thesis includes description of different types of workpiece handling. The second part is formed by bar feeders description and distribution and possibly construction solution. In the last part is performed choice of parameters for designed bar feeder and design of short bar feeder construction itself.

This thesis deals with design of right-angled elongated milling head for horizontal boring machine of FERMAT production. The thesis includes a detailed research of milling heads, a description of the head´s basic components and certain commercially available head´s comparison. Design part is describe components description and the design. Calculations are focused on determinating of forces generated during machining, setting of loading spectrum and the power load calculating individual gears during machining. In the next part is calculation of the reactions in the spindle bearings included, the equivalent load and durability of spindle bearings, spindle deformation and spindle bearings optimum distance. The thesis also includes spindle strength calculation, horizontal and vertical shafts analysis, gears design with helical teeth and gear strength. The thesis describes the design of extended milling head and particular design nodes which is supplemented by the 3D model. In this thesis is also included a drawing of assembly and components.