O câncer de mama é importante problema de saúde pública. O microambiente onde as células cancerígenas se encontram possui moléculas como a laminina e seus peptídeos bioativos, que influenciam a biologia tumoral. Estudo anterior realizado no laboratório demonstrou que o peptídeo C16, derivado da laminina, aumenta a expressão gênica de GPNMB e SPOCK1. Nesse trabalho, demonstramos que o peptídeo C16 aumentou níveis moleculares de GPNMB e SPOCK em células malignas MDA-MB-231e MCF-7, comparado com células normais MCF-10A. C16 estimulou significantemente a invasão de células MDA-MB-231. Silenciamento de GPNMB diminuiu a invasão celular desencadeada por C16. Contextualizando in vivo nossos resultados in vitro, imunohistoquímica em tissue microarrays mostrou que a presença de GPNMB e SPOCK é significantemente maior em câncer de mama. Assim, C16 regula os níveis de GPNMB e SPOCK em células mamárias malignas. C16 e GPNMB cooperam regulando a invasão de células MDA-MB-231. GPNMB e SPOCK foram mais detectados em câncer de mama comparado com mama normal. / Breast cancer is an important public health. The microenvironment in which cancer cells are found contains molecules such as laminin and its bioactive peptides that influence tumor biology. Previous study conducted in the laboratory showed that the C16 peptide derived from laminin, increases the gene expression of GPNMB and SPOCK1. In this work, we demonstrate that the C16 peptide increased molecular levels of GPNMB and SPOCK in malignant cells MDA-MB-231e MCF-7 cells compared with normal cells MCF-10A. C16 significantly stimulated invasion of MDA-MB-231 cells. GPNMB silencing decreased cell invasion triggered by C16. Contextualizing in vivo our in vitro results, tissue microarrays immunohistochemistry showed that the presence of GPNMB and SPOCK are significantly higher in breast cancer. Thus, C16 regulates the levels of GPNMB and SPOCK in malignant breast cells. C16 and cooperate GPNMB regulating the invasion of MDA-MB-231 cells. SPOCK and GPNMB were more detected in breast cancer compared to normal breast.

O câncer de mama é importante problema de saúde pública. O microambiente onde as células cancerígenas se encontram possui moléculas como a laminina e seus peptídeos bioativos, que influenciam a biologia tumoral. Estudo anterior realizado no laboratório demonstrou que o peptídeo C16, derivado da laminina, aumenta a expressão gênica de GPNMB e SPOCK1. Nesse trabalho, demonstramos que o peptídeo C16 aumentou níveis moleculares de GPNMB e SPOCK em células malignas MDA-MB-231e MCF-7, comparado com células normais MCF-10A. C16 estimulou significantemente a invasão de células MDA-MB-231. Silenciamento de GPNMB diminuiu a invasão celular desencadeada por C16. Contextualizando in vivo nossos resultados in vitro, imunohistoquímica em tissue microarrays mostrou que a presença de GPNMB e SPOCK é significantemente maior em câncer de mama. Assim, C16 regula os níveis de GPNMB e SPOCK em células mamárias malignas. C16 e GPNMB cooperam regulando a invasão de células MDA-MB-231. GPNMB e SPOCK foram mais detectados em câncer de mama comparado com mama normal. / Breast cancer is an important public health. The microenvironment in which cancer cells are found contains molecules such as laminin and its bioactive peptides that influence tumor biology. Previous study conducted in the laboratory showed that the C16 peptide derived from laminin, increases the gene expression of GPNMB and SPOCK1. In this work, we demonstrate that the C16 peptide increased molecular levels of GPNMB and SPOCK in malignant cells MDA-MB-231e MCF-7 cells compared with normal cells MCF-10A. C16 significantly stimulated invasion of MDA-MB-231 cells. GPNMB silencing decreased cell invasion triggered by C16. Contextualizing in vivo our in vitro results, tissue microarrays immunohistochemistry showed that the presence of GPNMB and SPOCK are significantly higher in breast cancer. Thus, C16 regulates the levels of GPNMB and SPOCK in malignant breast cells. C16 and cooperate GPNMB regulating the invasion of MDA-MB-231 cells. SPOCK and GPNMB were more detected in breast cancer compared to normal breast.

This bachelor thesis presents a comparison of three Java testing frameworks - JUnit 5,TestNG and Spock - with the purpose of evaluating their suitability in testing RESTAPIs built with Spring Boot. As the demand for reliable and high-quality softwaresystems continues to grow, automated testing techniques are crucial in ensuring thecorrect functionality of applications. Our study aims to fill the knowledge gap in thecurrent literature by focusing on unit tests for Java REST APIs running on the Springframework.We developed a single Spring Boot application and applied tests written using thethree selected testing frameworks. We then compared the performance of the frame-works based on execution time, memory usage and code conciseness. Additionally,we conducted a questionnaire to gather developer preferences for the frameworks.Our findings reveal that TestNG outperforms JUnit 5 in terms of performance, whileSpock requires fewer characters, making it more concise. However, JUnit 5 remainsthe most well-known and widely used testing framework among developers. Theresults of our study provide valuable insights into the performance and developerpreferences of the selected testing frameworks.