Thesis (Ph. D.)--City University of New York, 1978. / "Published articles and letters by Paul Strand: 1917-1931": leaves 254-255. Includes bibliographical references (leaves 367-390).

Thesis (M.A.)--Ohio State University, 2005. / Includes bibliographical references (leaves 84-86). Available online via OhioLINK's ETD Center

Thesis (Ph. D.)--City University of New York, 1978. / "Published articles and letters by Paul Strand: 1917-1931": leaves 254-255. Includes bibliographical references (leaves 367-390).

Submitted by Anderson Montagner Martins (anderson.montagner@gmail.com) on 2018-11-14T02:37:15Z No. of bitstreams: 1 Anderson Montagner Martins - Dissertação Mestrado (2018).pdf: 10796603 bytes, checksum: 697d340d41d25e2369055c39794cbbdb (MD5) / Approved for entry into archive by Maria Luiza Carpi Semeghini (luiza@assis.unesp.br) on 2018-11-14T22:39:43Z (GMT) No. of bitstreams: 1 martins_am_me_assis_int.pdf: 10796603 bytes, checksum: 697d340d41d25e2369055c39794cbbdb (MD5) / Made available in DSpace on 2018-11-14T22:39:43Z (GMT). No. of bitstreams: 1 martins_am_me_assis_int.pdf: 10796603 bytes, checksum: 697d340d41d25e2369055c39794cbbdb (MD5) Previous issue date: 2018-09-26 / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Em 1933, a Secretaría de Educación Pública (SEP) do México criou o seu Plan para la filmación de películas educativas, um projeto cinematográfico financiado pelo Estado que tinha como objetivo produzir uma série de filmes com o intuito de se forjar uma consciência socioeconômica na população. O projeto foi criado no mesmo período em que o Partido Nacional Revolucionario (PNR) elaborou o seu Plan Sexenal, documento que continha as diretrizes governamentais do mandato de seu candidato oficial, Lázaro Cárdenas, vitorioso nas eleições de 1934. Entre as medidas propostas pelo PNR estava a polêmica reforma educacional que implantou a “educación socialista” no país. O foco central da nossa pesquisa é o único filme concluído do projeto cinematográfico da SEP, Redes, produzido entre os anos 1934 e 1936. O filme narra a luta de um grupo de pescadores contra as injustiças impostas por um comerciante e um candidato político. A mensagem de Redes é bem clara: somente através da união entre os trabalhadores é possível superar a exploração da qual são vítimas. Os principais envolvidos na criação de Redes foram o compositor e maestro mexicano Carlos Chávez (1899-1978), então chefe do Departamento de Bellas Artes da SEP e o fotógrafo nova-iorquino Paul Strand (1890-1976), que estava no México desde 1932 realizando um projeto fotográfico. Nossa hipótese é que Redes pode ser considerado tanto resultado de uma política cultural revolucionária da SEP, que se radicalizou após a criação do PNR, quanto uma obra pessoal de Paul Strand. O nosso objetivo é refletir como Redes se insere em tal contexto político e cultural e, através da análise fílmica, apontar as tensões e ambiguidades presentes no discurso cinematográfico. Com isso, pretendemos identificar as aproximações e as rejeições entre o filme e o projeto ideológico-estético, tanto da SEP quanto de Paul Strand. / In 1933, Mexico's Secretaría de Educación Pública created its Plan para la filmación de películas educativas, a state-funded cinematographic project that aimed to produce a series of films promoting socioeconomic awareness in the population. The project was created in the same period in which Partido Nacional Revolucionario (PNR) drew up its Plan Sexenal, which contained the government guidelines of the mandate of its official candidate, Lázaro Cárdenas, victorious in the 1934 elections. Among the measures proposed by PNR was a controversial education reform that implanted the “educación socialista” in the country. The focus of our research is the only completed film from SEP’s cinematographic project, Redes, produced between 1934 and 1936. The film focuses on the struggle of a group of fishermen against the injustices imposed by a merchant and a political candidate. The message of Redes is very clear: only through their union, the workers can overcome the exploitation of which they are victims. The main people involved in the creation of Redes were the Mexican composer and conductor Carlos Chávez (1899-1978), then head of SEP's Departamento de Bellas Artes, and the New Yorker photographer Paul Strand (1890-1976), who was in Mexico since 1932 doing a photographic project. Our hypothesis is that Redes can be considered as both a result of a revolutionary cultural policy of SEP, which became radicalized after the creation of PNR, and a personal work of Paul Strand. Our objective is to reflect how Redes is inserted into such a political and cultural context and, through film analysis, to point out the tensions and ambiguities seen in the cinematographic discourse. Thereby, we intend to identify the approximations and rejections between the film and the ideological-aesthetic project of both SEP and Paul Strand. / CNPq: 130160/2017-2

Nucleic acid amplification is responsible for pushing the limit-of-detection of molecular diagnostic assays to unprecedented levels. We developed an assay based on protein-responsive programmable dynamic DNA assembly (PRPDA) to detect proteins via an intermediate process involving nucleic acids for taking advantage of nucleic acid amplification strategies. PRPDA has previously been designed for sensitive protein analysis in fluorescent assay formats. To further push the detection limit and to achieve assay miniaturization and multiplexing, we sought to combine PRPDA with electrochemical readout. We were able to achieve LOD of 1 pM by employing wrinkled gold electrode for the PRDA protein detection scheme. Which is 2800 times improvement compare to the 2.8 nM demonstrated by fluorescent transduction. / Thesis / Master of Applied Science (MASc)

The first objective of this thesis was to determine the material properties of grade 270 and grade 300 prestressing strand of various sizes. Tension tests were performed on each type of strand. The data from these tests was used to determine modulus of elasticity, yield stress, ultimate stress, and ultimate elongation for each strand. The yield stresses and ultimate stresses for many of the strands did not meet the requirements found in ASTM A416. The ultimate elongation results far exceeded the requirements and the measured elastic moduli were near the modulus recommended by AASHTO LRFD. A secondary objective from the tension tests was to evaluate a gripping method which used aluminum tubing to cushion the strands against notching. The grips performed very well. Most of the strand breaks did not occur in the grips and when a strand did break in the grips, the failure occurred after significant post-yield elongation. The second objective was to evaluate the bond properties of grade 270 and grade 300 prestressing strands. The North American Strand Producers (NASP) Bond Test and Large Block Pullout Test (LBPT) were performed on six different strand grade and strand size combinations. Both of the tests are simple pullout tests on untensioned strand. The results for each strand type were compared to one another as well as to measured transfer and development lengths from beams using the strand from the same reel. All of the strands showed sufficient bond in the beams, but one strand type did fail both the NASP Test and the LBPT. Both pullout tests were acceptable methods to evaluate strand surface condition and the benchmarks set for 0.5 in. diameter regular strand were conservative for the strands used in this thesis. Little difference was evident in the bond performance of grade 270 and grade 300 prestressing strand. / Master of Science

The tumor suppressor Breast Cancer Susceptibility Protein 1 (BRCA1) protects our cells from genomic instability in part by facilitating the efficient repair of DNA double-strand breaks. Other functions of BRCA1 include transcriptional regulation, apoptosis, DNA damage signaling, chromatin remodeling and protein ubiquitination. The major contribution of BRCA1 to maintaining genomic stability is thought to be through its role in DNA repair. BRCA1 promotes the error-free repair of double-strand breaks by homologous recombination, and is also implicated in the regulation of non-homologous end joining repair. Here we investigated the role of BRCA1 in maintaining the fidelity of non-homologous end joining repair following a double-strand break. We also examined the frequency of microhomology-mediated end joining (MMEJ) and the fidelity of double-strand break repair relative to BRCA1 protein levels in both control and tumorigenic breast epithelial cells. In addition to altered BRCA1 protein levels, we tested the effects of cellular exposure to mirin, an inhibitor of Meiotic recombination enzyme 11 (Mre11) 3' to 5' exonuclease activity. Knockdown or loss of BRCA1 protein resulted in an increased frequency of overall plasmid DNA repair mutagenesis and MMEJ following a double-strand break. Inhibition of Mre11 exonuclease activity with mirin significantly decreased the occurrence of MMEJ, but did not considerably affect the overall mutagenic frequency of plasmid double-strand break repair, although some of our data indicate that the size of sequence deletions may be reduced by mirin inhibition. The results suggest that BRCA1 protects DNA from mutagenesis during non-homologous double strand break repair in plasmid-based assays. The increased frequency of double-strand break mutagenesis and MMEJ repair in the absence of BRCA1 suggests a potential mechanism for carcinogenesis.

No description available.

No description available.

DNA polymerases play a fundamental role in the transmission and maintenance of genetic information and have become an important in vitro diagnostic and analytical tool. The Loop-mediated isothermal DNA amplification (LAMP) method has major applications for disease and pathogen detection and utilises the unique strand-displacement activity of a small group of thermostable DNA polymerases. The Large (Klenow-like) Fragment of Geobacillus stearothermophilus DNA polymerase I (B.st LF Pol I) currently serves as the enzyme of choice for the majority of these isothermal reactions, with few alternatives commercially available. An increasing need for point-of-care nucleic acid diagnostics is now shifting detection methods away from traditional laboratory based chemistries, such as the polymerase chain reaction (PCR), in favour of faster, and often simpler, isothermal methods. It was recognised that in order to facilitate these rapid isothermal reactions there was a requirement for alternative thermostable, strand-displacing DNA polymerases and this was the basis of this thesis. This thesis reports the successful identification of polymerases from Family A, chosen for their inherent strand-displacement activity, which is essential for the removal of RNA primers of Okazaki fragments during lagging-strand DNA synthesis in vivo. Twelve thermophilic organisms, with growth temperature ranges between 50oC and 80oC, were identified and the genomic DNA extracted. Where DNA sequences were unavailable, a gene-walking technique revealed the polA sequences, enabling the Large Fragment Pol I to be cloned and the recombinant protein over-expressed in Escherichia coli. A three-stage column chromatography purification permitted the characterisation of ten newly identified Pol I enzymes suitable for use in LAMP. Thermodesulfatator indicus (T.in) Pol I proved to be the most interesting enzyme isolated. Demonstrating strong strand-displacement activity and thermostability to 98oC, T.in Pol I is uniquely suitable to a newly termed heat-denaturing LAMP (HD-LAMP) reaction offering many potential advantages over the existing LAMP protocol. The current understanding of strand-displacement activity of Pol I is poorly understood. This thesis recognised the need to identify the exact regions and motifs responsible for this activity of the enzyme, enabling potential enhancements to be made. Enzyme engineering using site-directed mutagenesis and the formation of chimeras confirmed the importance of specific subdomains in strand-separation activity. With this knowledge, a unique Thermus aquaticus (T.aq) Pol I mutant demonstrated sufficient strand-displacement activity to permit its use in LAMP for the first time. The fusion of Cren7, a double-stranded DNA binding protein, to Pol I for use in LAMP is also reported. Although the fusion construct was found to reduce amplification speed, enhancements were observed in the presence of increased salt concentrations and it is suggested here as a means for future enzyme development.