A compost consisting 95% of the sugar mill byproducts: bagasse, filter press mud, and fly ash, applied at 5 t ha$ sp{-1}$, increased vesicular-arbuscular mycorrhizal infection of sugarcane roots in one of three experimental fields in Barbados. In a plant cane field, compost stimulated formation of intracellular hyphal coils and arbuscules, but not vesicles or hyphae. Infection was greater in roots 35 cm and 65 cm than 5 cm distant from the plant stem, and compost effects were not significant at 5 cm. Two other sources of phosphorus, filter press mud and triple superphosphate, did not affect and suppressed mycorrhizal infection, respectively. Two ratoon crops showed no residual effect of compost on mycorrhizal infection. Compost also stimulated tillering, phosphorus content, and perhaps yield of cane, but did not differentially effect high versus low tillering or sloped versus flat areas. Root length, weight, and specific root length were unaffected by compost addition, but root branching was decreased.

The biochemical basis for the regulation of sucrose accumulation is not fully understood. The present study was thus aimed at investigating aspects of 'coarse' (enzyme activity) and 'fine' (metabolite) control of glycolytic enzyme activity in relation to carbon partitioning in the developing stalk (internodes 3 to 10), and between varieties with significant differences in sucrose content. Particular emphasis was placed on studying pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90), since this enzyme has been implicated in sucrose metabolism in other plant species. Within the developing stalk, internodal maturation was associated with a redirection carbon from the insoluble matter and total respiration (C02 production and biosynthesis) to sucrose storage. Between varieties, with significant variation in sucrose content, there was an inverse relationship between hexose monophosphate partitioning into respiration and sucrose. The reduction in carbon flux to respiration was not associated with a decline in the extractable specific activity of PK, PFK and PFP. There was also no alteration in the regulation of PK, PFK and FBPase by change in the mass action ratios. Hexose monophosphate concentration declined approximately two to three-fold from internodes 3 to 9 and Fru-6-P concentration was within the lower Km or 80.5 range (Fru-6-P) of PFP and PFK, respectively (as reported from the literature) . Within the developing stalk, substrate limitation might have contributed to the decline in carbon partitioning to respiration. In sugarcane, the levels of PFP activity were controlled in part by PFP protein expression. 8ugarcane PFP polypeptide(s) are resolved as a single protein with a molecular mass of approximately 72 kO. PFP catalysed a reaction close to equilibrium in all intemodes investigated, and the concentration of Fru-2,6-P2 was shown to be in excess of the requirement to stimulate PFP activity. Carbon flux from the triose-P to hexose monophosphate pool was apparent in sugarcane, suggesting that PFP activity was functional in vivo. The developmental profile of specific PFP activity was not positively correlated to the increasing rate of sucrose accumulation in the top ten internodes of the developing stalk. Between different sugarcane varieties, specific PFP activity was shown to be inversely correlated to sucrose content. / Thesis (Ph.D.)-University of Natal, Durban, 1997.

As is the case in many other regions in the world, sugarcane production in southern Africa is affected by a wide range of climatic conditions, which can vary considerably from location to location and from year to year. As a result, the season length and growth cycles of sugarcane in southern Africa differ greatly. Such conditions include the hot and dry regions of northern KwaZulu-Natal, Swaziland and Mpumalanga, where sugarcane is mostly irrigated, to the humid sub-tropical coastal belt extending from the far north coast of KwaZulu-Natal to areas in the Eastern Cape, as well as the cool frost prone midlands regions of KwaZulu-Natal. Owing to the wide range of climatic conditions in which sugarcane is grown in southern Africa, there are many different external factors that affect sugarcane production, including a range of pests and diseases, frost occurrences and variations in soil water. The objective of this research was to (1) identify a number of important variables that affect cane production in southern Africa, (2) employ suitable models to reflect these variables, and (3) simulate and map the extent and severity of these variables at a high spatial resolution over southern Africa. Such variables include the Eldana saccharina and Chilo sacchariphagus stalk borers, sugarcane rust fungus, heat units with selected base temperatures, frost, soil water content, soil compaction, irrigation water demand, conducive and non-conducive growing conditions, flowering proficiencies for sugarcane, sugarcane yields and yield increments per unit of irrigation. The distribution patterns of the above-mentioned variables relied greatly upon the various models employed to represent them, as well as the accuracy of the temperature and rainfall databases to which the various models were applied. Although not definitive, the models used to reflect the variables which had been identified were considered to be generally satisfactory. The resolution at which the variables which had been identified in this study were mapped, was also found to be adequate. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

The South African sugar industry ranks eleventh in size out of 200 sugar-producing countries; and continuous advancement is essential to ensure that this industry remains competitive. The transfer system from field to mill, includes sugarcane being moved, loaded, transloaded and off-loaded and amounts to more than 25 % of the total production cost of sugarcane, hence small adjustments can have significant economic benefits. Payload variability is a current problem making the loading operation a leverage point for improvement. As a consequence of poor management and the under-utilisation of equipment, loading has been identified as an inefficient and costly operation. Studies have shown that technology and management can contribute to improved loading accuracy. This study aimed to evaluate loading characteristics in an attempt to develop guidelines for loading. Whole-stick loose sugarcane, which is loaded with grabloaders, is common practice in South Africa and this study aims to improve this loading system. This was achieved by reviewing transfer systems worldwide and synthesising the sugarcane characteristics that drive the designs and the management of equipment and systems within the transfer system. The sugarcane characteristics include the sugarcane bulk density, the length, the diameter and other variety characteristics e.g. the degree of lodging. Other factors, such as the preparation method for loading, the harvesting method and the topography, also have a marked influence on the efficiency of the transfer system. An assessment of typical South African loading practices was undertaken to establish beneficial practices and current operating rules. These factors included the way in which the sugarcane was presented prior to loading, as well as the techniques adopted for loading. The results were used to generate practical recommendations for the improvement of the loading component in order to make the transfer system more efficient. An efficient system comprises a balance of high quality operations with respect to safety, accurate loading, reduced cycle time, optimal fuel usage, reduced roadside losses, reduced sugarcane damage and increased off-loading efficiencies. An investigation into consignment characteristics was also carried out to identify the factors that need to be considered during the loading operation. These factors included the sugarcane bulk density, the degree of sugarcane alignment and the design characteristics of various vehicles. A set of practical guidelines were created from this study. / Thesis (M.Sc.)-University of KwaZulu-Natal, Durban, 2011.

The purpose of this study was to develop a framework to assess irrigation design and operating strategies. This objective was achieved successfully and the framework was applied to formulate guidelines to increase farm profitability whilst using scarce resources, such as water and electricity, effectively. The study was targeted at sugarcane irrigated with semi-permanent irrigation systems. “ZIMsched 2.0”, a water balance and crop yield prediction model and the “Irriecon V2” economic assessment model were available at the start of the study. The missing link, however, was a relatively cost effective and efficient method to design and cost irrigation hardware alternatives. Irrigation hardware impacts on both the agronomic and economic performance of systems, for example, through different peak design capacities and associated operating limitations. Thus, a novel, spreadsheet-based irrigation design tool, with an automated costing component, was developed to complete the framework. The framework was used to investigate the costs and benefits of potential design and operating solutions to a selection of irrigation issues, including: over-irrigation on shallow soils, the opportunity to shift electricity use out of expensive peak periods and, the opportunity to demonstrate the benefits of deficit irrigation strategies. For shallow soils, the increase in system hardware costs, needed to better match water application to soils, increased margins due to more effective water use. Innovative deficit designs and operating strategies allowed for reductions in water and electricity costs. The reduced costs, however, did not always offset yield penalties and revenue loss resulting from water stress. The financial benefits of deficit irrigation strategies were shown when water savings were used to convert dry land cane into irrigated cane. This highlighted the differences between the direct and opportunity costs of water. Finally, a field work component, relating to the precise monitoring of irrigation strategies and corresponding crop responses was included in this study. Systems which enabled soil water potential and stalk extension to be monitored remotely via the internet were considered useful for the successful implementation of an optimum irrigation strategy. The easily accessible data allows for effective decision making and more importantly, reassures famers of the current state of their crop. / Thesis (M.Sc.)-University of KwaZulu-Natal, Durban, 2009.

The rising cost of energy coupled with an increasing awareness of Greenhouse Gas (GHG) emissions has led to a concerted effort to reduce fossil fuel Energy Use (EU) in all sectors. Sugarcane production in South Africa is dependent on fossil fuel to provide a source of energy for production. To remain commercially and environmentally sustainable, measures need to be taken to reduce EU and increase EU efficiencies of on-farm operations. The first step toward realising this is to identify and quantify energy inputs. Following on from this, total GHG emissions, also known as carbon footprint, can be estimated. The primary objective of this research is to develop an energy calculator to estimate EU in sugarcane production in South Africa. The results generated by the calculator highlight areas of high energy intensity and low energy efficiencies at three different levels of detail. Based on these results, changes in management practices and technological improvements can be made to reduce EU and carbon footprint. Case studies were used to test the functionality of the calculator. Results from the case studies show that, in irrigated sugarcane production, the harvest and transport process together with irrigation account for a majority of the total on-farm EU. For one of the case studies, an estimated 20 % saving in the total on-farm EU was identified and can be achieved if appropriate technology is adopted in irrigation practices. Less significant energy savings were realised when in-field tractor operations were optimised for best tractor-implement matching. It is envisaged that the energy calculator will help farmers minimise on-farm EU and subsequently reduce input costs and carbon footprint. It will also provide a valuable tool for researchers to benchmark and profile EU in sugarcane production in South Africa. Research focussed on the sustainable production of sugar, from the agricultural to milling phase is of high priority at present. The quantification of on-farm EU in sugarcane production will form a critical component of such research. / M.Sc.Eng. University of KwaZulu-Natal, Pietermaritzburg 2014.

Post-harvest cane deterioration in the South African sugar industry results in significant revenue loss that is estimated to be in the region of ZAR 60 million per annum. Despite these large losses, precise biological data relating to the process of cane deterioration under South African conditions is limited. Severity of deterioration is influenced by a number of factors, including the length of the harvest-to-crush delay (HTCD), ambient temperature and harvesting practices. For example, burning of cane prior to harvest may result in rind splitting, which provides entry for microbes, particularly Leuconostoc mesenteroides that may exacerbate deterioration. The effect of these factors on deterioration was examined by quantifying the biochemical and microbiological changes that occur in sugarcane stalks after harvest, with the influence of length of HTCD, degree of L. mesenteroides infection and ambient temperature receiving attention. The primary novelty of the work resides in the analysis of deterioration under tightly regulated temperatures, which were designed to reflect diurnal variations typically experienced during summer and winter in the South African sugar belt. In addition, inoculation of mature internodes with a consistent titre of L. mesenteroides was used as a means to mimic a consistent level of infection of harvested stalks by the bacterium. Metabolites selected for analysis were those both native to the stalk and produced as by-products of microbial metabolism, viz. sucrose, glucose, fructose, ethanol, lactic acid, dextran and mannitol. Simulated HTCDs under summer temperatures resulted in increasing glucose and fructose levels with time, which contrasted to the approximately constant levels of these hexose sugars under winter conditions. Commonly referred to as ‘purity’ in an industrial context, precise determination of the concentration of these hexoses in cane consignments could potentially indicate the extent of deterioration. Despite the detection of a basal concentration of lactic acid in unspoiled cane, the observed increase in concentration of this organic acid over the simulated summer HTCD suggests that this metabolite could also potentially serve as an indicator for postharvest deterioration. In contrast, the investigation indicated that ethanol was an unsuitable biochemical marker for deterioration of L. mesenteroides infected cane. An inability to detect dextran and mannitol in the samples, combined with consistent sucrose levels and variable mill room data, suggest that extreme proliferation of L. mesenteroides is facilitated primarily by in-field practices, particularly the manner in which cane is prepared prior to harvest and transport to the mill. Bacterial proliferation and infection by L. mesenteroides of inoculated stalks were monitored by standard selective culturing techniques. Despite the limited detection of L. mesenteroides-associated metabolites, culture-based analyses revealed that the bacterium was the dominant bacterial species within the samples. A number of other bacterial species were isolated and identified, however the extent to which the total number of microorganisms proliferated was limited to a maximum of 1 x 105 colony forming units per gram of fresh tissue. In conjunction with these analyses, a molecular approach known as Polymerase Chain Reaction-Mediated Denaturing Gradient Gel Electrophoresis (PCR-DGGE) was undertaken to investigate the bacterial diversity patterns associated with deteriorating sugarcane stalks throughout the delay period. In contrast to the results obtained by means of the culture-based assays, PCR-DGGE revealed that L. mesenteroides was not the dominant bacterial population, and showed that the level of bacterial diversity was relatively consistent across the differing treatments and with time. The use of complimentary culture-dependent and cultureindependent analyses thus permitted the detection of this discrepancy and indicated the utility of PCR-DGGE in the determination of bacterial community structure of postharvest sugarcane tissue. The biology of post-harvest deterioration of green sugarcane stalks is highly complex, even under rigorously controlled temperature and infection regimens. The results of this study emphasize the important effects that harvest method and environmental conditions have on post-harvest sugarcane deterioration. Towards the formulation of industry-relevant recommendations for combating post-harvest deterioration, future work will strive to mimic the effects that harsh harvesting and transport practices have on the severity of the problem. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1979. / Bibliography: leaves 145-157. / Microfiche. / xii, 157 leaves ill. 29 cm

Thesis (Ph.D.)--University of Hawaii at Manoa, 1994. / Includes bibliographical references (leaves 97-114). / Microfiche. / xiii, 114 leaves, bound ill. 29 cm

In 1931, Mungomery stated "whitegrubs have been for years past, and still are, the worst insect problem confronting the sugar industry". This statement remains true to this day, with canegrubs costing the Australian sugar industry A$7.22 million in lost production and in use of insecticides. The development of a sugarcane cultivar with resistance to canegrub attack would be a valuable addition to the recently implemented canegrub management program. This thesis examined the possibility that natural inhibitors derived from canegrubs could be incorporated in sugarcane to reduce or prevent its destruction by canegrubs. The research described here demonstrated that canegrub haemolymph contains inhibitors with activity against commercially purified enzymes and serine proteases found in crude midgut extracts. A cDNA encoding a potential canegrub protease inhibitor (DA10 12) belonging to the Ascaris family was cloned, but it did not have activity against the major canegrub midgut proteases. This protein does, however, still have potential for modification into a serine protease inhibitor suitable for use as a novel insect resistance transgene. The possibility of using haemolymph derived inhibitors as novel antimetabolites in a canegrub management strategy based on transgenic plants was also explored. The findings suggest that proteins with properties similar to those of DA10 12 will require the presence of a signal peptide and/or codon optimisation for successful expression in sugarcane. The research outlined in this thesis is the first investigation of protease inhibitors in the haemolymph of scarab larvae, and is the first report of an Ascaris family inhibitor that does not inhibit a serine protease.