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Evaluation of substrates for surface-enhanced Raman scatteringZhong, Muyang 15 August 2016 (has links)
Surface-enhanced Raman scattering (SERS) has long been the interest of researchers in chemistry, physics and engineering, especially since the discovery that SERS can probe into the system down to the single molecule (SM) level. Despite the large number of publications regarding the fabrication of SERS substrates, it has been a challenge in the field to quantify the SERS signal and universally compare substrates. Traditionally, enhancement factor (EF) is used as an indicator of substrate quality, but the EF calculation is hugely dependent on the estimation of the surface coverage and other factors that are determined largely subjectively. Therefore, this thesis aims at discussing other parameters that can also be used to evaluate different substrates.
Six different SERS substrates of Ag or Au nanoparticles of different sizes were fabricated by nanosphere lithography (NSL) and characterized by electron microscopy and UV-vis spectroscopy. SERS substrates were mapped for different concentrations of a probe molecule. Through subsequent baseline correction and principle component analysis (PCA), the "intensity" of individual spectrum was obtained and the shapes of intensity histograms of each substrate were acquired.
Instead of calculating EF, five criteria (six quantification methods in total) were employed to comprehensively evaluate the six substrates. These were density of hot spots (characterized by the number of zero-intensity events), enhancement (represented by mean intensity), spatial variation (calculated by RSD of intensity), repeatability (realized by cross correlation) and histogram shape (quantified by skewness and kurtosis). These new methods provide insights to the understanding of the properties of SERS substrates in terms of hot spots. Different substrates may exhibit better performance in terms of one criterion but worse in terms of others. Those variations in performance can be explained by their surface morphology.
These more elaborated methods are believed to provide a more comprehensive approach to evaluate and compare substrates than the traditional EF values. The thesis also paves the way for future study on SM-SERS and fabricating better SERS substrates. / Graduate
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Surface-enhanced raman scattering and surface-enhanced hyper raman scattering : a systematic study of various probing molecules on novel substrates /Huang, Qunjian. January 2003 (has links)
Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2003. / Includes bibliographical references. Also available in electronic version. Access restricted to campus users.
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Surface-enhanced hyper raman and surface-enhanced raman scattering : novel substrates, surface probing molecules and chemical applications /Xie, Yu-Tao. January 2007 (has links)
Thesis (Ph.D.)--Hong Kong University of Science and Technology, 2007. / Includes bibliographical references. Also available in electronic version.
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Metallic nanoparticles: analytical properties of the acoustic vibrations and applicationsWu, Jian 16 August 2017 (has links)
This thesis focuses on the analytical properties of the acoustic vibrations and applications of metallic nanoparticles. With regard to the analytical properties of the acoustic vibrations, we focus on nanoparticle acoustic resonance enhanced four-wave mixing (FWM) as an in situ characterization technique for characterizing nanoparticles’ shape, size, and size distribution. The nonlinear optical response of metallic nanoparticles is resonantly driven by the electrostriction force which couples to the acoustic vibrations of nanoparticles. Information about nanoparticles’ shape, size, and size distribution can be obtained by analyzing the resonant peak position and linewidth in the FWM signal which carries the information about the vibrational modes. We characterize different nanoparticle solutions of different materials, shapes, and sizes using this FWM technique. Information obtained from the FWM characterization agrees well with the scanning electron microscopic examination, indicating the FWM technique can serve as an in situ nanoparticle characterization tool. We also demonstrate the FWM technique can be used for monitoring nanoparticle growth in situ.
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With regard to the applications of metallic nanoparticles, we focus on quantification of an exogenous cancer biomarker Acetyl Amantadine using surface-enhanced Raman scattering (SERS). Raman spectroscopy can provide unique fingerprint information of molecules, which can be used as a chemical detection and identification technique. The intrinsically weak Raman signal caused by the small scattering cross section presents a barrier for trace chemical detection. Localized surface plasmon resonance of metallic nanoparticles can provide large local field enhancement, which can be utilized to enhance the intrinsically weak Raman signal. In order to achieve higher local field enhancement, we focus on using the gap structures formed between nanoparticles instead of using discrete nanoparticles. Molecules should locate within the hot spots of the gap structures to experience the largest enhancement. This requires that molecules should be extracted from volume onto the metallic surface. Based on these guidelines, two SERS platforms are designed using gold nanoparticles (nanorods and nanospheres) combined with different surface functionalization techniques. The performance of these two platforms are characterized by investigating the sensitivity and limit of detection (LOD). 16 ng/mL and 0.4 ng/mL LODs are achieved for nanorod and nanosphere platforms, respectively. / Graduate
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Optimisation of solid-state and solution-based SERS systems for use in the detection of analytes of chemical and biological significanceMabbott, Samuel January 2013 (has links)
Surface enhanced Raman scattering (SERS) has achieved much attention since its conception in 1974. The analytical technique overcomes many difficulties associated with conventional Raman whilst also increasing sensitivity. However, the increased interest and work in the field has also identified flaws, many of which are centred on the irreproducibility of the SERS enhancement effect. The majority of the work described in this thesis focusses on the ‘optimisation’ of solid-state and solution based SERS systems. Optimisation plays a crucial role in maximising both enhancement effects and reproducibility. Here criteria are outlined for the synthesis of high performance solid-state SERS substrates and the synthesis of a range of substrates is assessed, each with associated pros and cons. The most successful substrate was synthesised by exploiting redox potentials which allow for the direct deposition of silver onto copper foil. The deposition times and temperatures were optimised sequentially to generate a high performance substrate capable of detecting Rhodamine 6G at trace levels. Reproducibility comparisons of the silver on copper (SoC) substrate were carried out against commercial substrates: Klarite and QSERS, multiple univariate and multivariate methods were used to assess the substrates performance. The results confirmed that the SoC substrate performed better than both the commercial substrates. The work also highlights the importance of using multiple data analysis methods in order to assess the performance of a solid-state SERS substrate. Deposition of the silver surface was also successful on British 2p coins allowing the for the detection and discrimination of illegal and legal drugs when coupled with multivariate data analysis methods such as PCA and PLS. Solution based SERS analyses were also carried out successfully using different optimisation strategies. The initial investigation involved careful control of the individual components of a SERS system (nanoparticles, aggregating agents and analyte) in order to establish a low limit of detection for the increasingly abused ‘legal high’ MDAI. The use of a reduced factorial design was then successfully employed to explore a greater number of SERS variables and define a low limit of detection for the class B drug mephedrone. The robust experimental design also allowed an insight into the importance of each of the individual components within a solution based SERS system. The final piece of work carried out was the SERS discrimination of antibiotics: ampicillin, ticarcillin and carbenicillin. Optimisation of the solution based experiment allowed the in-situ hydrolysis of the β-lactam moiety present in ampicillin rendering it pharmacologically inactive to be followed under acidic conditions at concentrations of 10 ppm.
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Rationally designed substrates for SERS biosensingYan, Bo January 2013 (has links)
Thesis (Ph.D.)--Boston University / The large electromagnetic field enhancement provided by nanostructured noble metal surfaces forms the foundation for a series of enabling optical analytical techniques, such as surface enhanced Raman spectroscopy (SERS), surface enhanced IR absorption spectroscopy (SEIRA), surface enhanced fluorescent microscopy (SEF), to name only a few. Critical sensing applications have, however, other substrate requirements than mere peak signal enhancement. The substrate needs to be reliable, provide reproducible signal enhancements, and be amenable to a combination with microfluidic chips or other integrated sensor platforms. These needs motivate the development of engineerable SERS substrate "chips" with defined near- and far-field responses. In this dissertation, two types of rationally designed SERS substrates - nanoparticle cluster arrays (NCAs) and SERS stamp - will be introduced and characterized. NCAs were fabricated through a newly developed template guided self-assembly fabrication approach, in which chemically synthesized nanoparticles are integrated into predefined patterns using a hybrid top-down/bottom-up approach. Since this method relies on chemically defined building blocks, it can overcome the resolution limit of conventional lithographical methods and facilitates higher structural complexity. NCAs sustain near-field interactions within individual clusters as well as between entire neighboring clusters and create a multi-scale cascaded E-field enhancement throughout the entire array. SERS stamps were generated using an oblique angle metal deposition on a lithographically defined piston. When mounted on a nanopositioning stage, the SERS stamps were enabled to contact biological surfaces with pristine nanostructured metal surfaces for a label-free spectroscopic characterization. The developed engineered substrates were applied and tested in critical sensing applications, including the ultratrace detection of explosive vapors, the rapid discrimination of bacterial pathogens, and the label-free monitoring of the enzymatic degradation of pericellular matrices of cancer cells.
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Nanolaminated Plasmonics: from Passive to Active Nanophotonics DevicesSong, Junyeob 09 June 2020 (has links)
Plasmonics can achieve the tight optical confinement and localization in the subwavelength domain. Surface plasmon polaritons (SPPs) are closely related to coupling to emitters in excitation and emission, waveguiding, and active modulating on the nanoscale. Due to these phenomenon, plasmonic nanostructures can be used for applications, such as light emission, photodetection, optical sensing, and spectroscopy. Conventional plasmonic nanostructures can support plasmonic modes, and it is typically optimized for a single wavelength window with planar plasmonic structures.
Recent studies have reported some in-plane composite nanostructures and core-shell geometries can induce multiple plasmonic responses. However, it is challenging to achieve the control of individual plasmonic response due to the interdependent spectral tunability with changes in their in-plane geometries. In this dissertation, the concept of out-of-plane engineered nanoantenna structures is introduced, numerically calculated, and experimentally demonstrated. The nanolaminated MIM plasmonic structures show multiresonant plasmonic responses in the same antenna and each wavelength band can be tunable individually with different thicknesses of dielectric layers. The nanolaminated plasmonic structures has been reported for a scalable Surface-enhanced Raman spectroscopy (SERS) substrate for single-molecule sensitive and label-free chemical analysis. Due to the strong optical field confinement, the nanolaminated SERS substrates achieve increased SERS enhancement factor (EF) up to 1.6 x 108 with proper partial etching of dielectric layers. Furthermore, the nanolaminated MIM plasmonic structures have been successfully integrated with micro-scale pillar arrays to control the surface wettability for ultrasensitive SERS measurements. The hierarchical micro/nano plasmonic surface has densely packed intrinsic SERS-active hot spots that give rise to SERS EFs exceeding 107. This platform can take full advantage of low surface energy to control and measure the analyte in water droplets. Leidenfrost evaporation-assisted SERS sensing on the hierarchical substrates provides the way for ultrafast and ultrasensitive biochemical detections without a need for additional surface modifications and chemical treatments. / Doctor of Philosophy / The life in the 21th century has benefited from the technical revolutions of computational power that is based on the manipulation/storage of electrons. As predicted in Moore's law, the size of electronic microchip would go down, and the computational power has been enhanced due to the increase of transistor integration density. However, the two major factors, such as energy dissipation of electrons and signal delay of electronic circuit, limit the communication speed of electronics. These barriers have caused slowdown in the performance of computational power.
Photonic solutions have been offered to solve the limitations based on the larger bandwidth and a rare energy dissipation, compared to electronic counterparts. Moreover, optical communications typically demand much lighter channel to deliver similar power/information than practical electrical cables do. Thus, light manipulation/enhancement techniques are envisioned to overcome the limitations and guide to the methodology of interconnections between the electronic circuits and optical platforms.
Plasmonics can achieve the nanoscale light confinement and localization in the subwavelength domain. This strong confinement is originated from the coupling between the photons and the electron gas on the metal that results in surface plasmon polariton (SPP). SPPs are closely related to coupling to emitters in excitation and emission, waveguiding, and active modulating on the nanoscale. Due to these phenomenon, plasmonic nanostructures can be used for applications, such as light emission, photodetection, optical sensing, and spectroscopy.
In this dissertation, the concept of out-of-plane engineered nanoantenna structures is introduced, numerically calculated, and experimentally demonstrated. This vertically stacked nanoantenna structure is composed of metal-insulator-metal (MIM) laminates fabricated by physical vapor deposition techniques. Although conventional plasmonic nanostructures can support plasmonic modes, it is typically optimized for a single wavelength window. The nanolaminated MIM nanostructures, by contrast, can induce multiresonant plasmonic response in the same antenna with several advantages: (1) reduced individual footprint size and volume of nanoantenna, (2) accurate control of layer thicknesses by thin film deposition technique for resonance tuning, (3) easier integration with other functional materials as gap layers, and (4) efficient transport of charge carriers or heat in nanolaminated layers.
As a result of the tight optical field confinement, the nanolaminated plasmonic structures can be used for sensing application called Surface-enhanced Raman spectroscopy (SERS), which is a promising sensing platform for label-free biochemical analysis at the single-molecule level. Partial oxide etching process enables the analyte molecules to accommodate in strong enhancement region of the nanolaminated structures, resulting in amplified unique Raman features of molecular compounds as a finger print. The SERS enhancement factor is increased by one order of magnitude achieving 1.6x108. Furthermore, the nanolaminated plasmonic structures have been integrated with micro-scale pillar arrays to control the surface wettability for ultrasensitive SERS measurements.
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Quantification of a lung cancer biomarker using surface enhanced Raman spectroscopyCao, Guangyi 24 December 2014 (has links)
Detecting lung cancer is di cult as it is hidden in the body, and current clinical methods are not elective at an early stage; the one-year survival rate after diagnosis in the World is just 29-33%. Acetyl amantadine (AcAm) is recognised as an exogeneous cancer biomarker because it is the product of a metabolic process known to be significantly up-regulated in cancerous cells. After ingestion, the an-tiparkinson and antiviral drug amantadine is acetylated in the body by the enzyme spermidine/spermine N1 acetyltransferase to give AcAm, which can be detected in patient’s urine. However, techniques previously used to quantify AcAm in urine, such as liquid chromatography-mass spectrometry (LC-MS), are undesirable for clin- ical adoption due to high costs and long run times. Further costs and delays result from the requirement for solid phase extraction (SPE). Therefore, it is highly desired to lower the costs and delays in processing by exploring different quantification approaches, ideally without the need for SPE processing.
In this thesis, I investigate the use of surface enhanced Raman spectroscopy (SERS) to quantify AcAm in urinalysis. I prepare two kinds of Raman substrates with hydrophobic pocket surface capture agents beta -cyclodextrin (beta -CD) that work to extract the AcAm from the urine, followed by the surface enhanced Raman measurement using two kinds of Raman systems. The detection strategy is more economical than the currently used LC-MS approach, and enables development of an easy-to-use point-of-care tool that should provide a more rapid turnaround to the health care provider. The next step will be to use real samples. If it is achieved, it will be a promising step in early cancer diagnostics. / Graduate
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Metal nanostructures for enhanced optical functionalities: surface enhanced Raman spectroscopy and photonic integration.Qiao, Min 01 September 2011 (has links)
As the developments in nanoscale fabrication and characterization technology, the investigation and applications of light in metal nanostructures have been becoming one of the most focused research areas. Metal materials allow to couple the incident light energy into electromagnetic waves propagating on the metal surface under certain configurations, which is called surface plasmon (SP). This feature tremendously expanded the application possibility of metals in optical regime, such as extraordinary transmission (EOT), near-field optics and surface enhanced spectroscopies. In this talk, various metal structures will be demonstrated which could control SP’s propagation, resonance andlocal field enhancement. A number of SP applications are benefited – the plasmonic bragg reflector (PBR), the frequency sensitive plasmonic microcavity, the subwavelength metallic taper, the long range surface plasmon (LRSP) waveguide and surface enhanced Raman spectroscopy (SERS). Especially for SERS, long-term effort was devoted into it to achieve the single molecule detection limit. / Graduate
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Surface-enhanced Raman spectroscopic studies of organonitriles on copper colloidsCoyle, Candace Mikki, January 1999 (has links)
Thesis (Ph. D.)--West Virginia University, 1999. / Title from document title page. Document formatted into pages; contains xvii, 169 p. : ill. Vita. Includes abstract. Includes bibliographical references.
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