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Native Mass Spectrometry for Characterization of Protein:Protein and Protein:RNA ComplexesJia, Mengxuan January 2019 (has links)
No description available.
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Characterization of Proteins and Protein Complexes by Online Chromatographic Separations and Direct Infusion Native Mass SpectrometrySahasrabuddhe, Aniruddha 02 August 2018 (has links)
No description available.
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Characterization of Self-Assembled Monolayers by Low Energy Reactive Ion Scattering: Influences of Terminal Group Composition and Structure on Ion-Surface InteractionYang, Xi January 2006 (has links)
Low energy (tens of eV) polyatomic cations were used as probes for characterization of monolayers of spontaneously chemisorbed thiols on gold. Characteristics including chemical composition, surface order and orientation of the self-assembled monolayers (SAMs) can be derived by monitoring the products of projectile ion neutralization, surface-induced dissociation (SID), and ion-surface reactions.To study the influence of the terminal group chemical structures and orientations of the SAMs on ion-surface interactions, a series of semi-fluorinated alkane thiols with difluoromethylenes buried underneath hydrocarbon terminal groups were examined (CH3CF2CH2− and CH3CH2CF2−). Compared to terminally fluorinated SAMs, they showed more projectile ion neutralization and less internal to vibrational energy deposition into precursor ions. Projectile ion-hydrocarbon reactions decreased significantly when difluoromethylenes are one or two bonds away from the terminal group. Furthermore, ion-surface reaction results on surfaces with odd and even chain lengths suggested that they have similar terminal methyl orientations to their hydrocarbon counterparts.Mixed monolayers of CF3CF2(CH2)14SH (F-SAMs) and CH3(CH2)15SH (H-SAMs) with systematically changing electron transfer, energy deposition and ion-surface reaction were prepared using mixed thiols solution and micro-contact printing (μ-CP). The solution mixture system showed linear variations in electron transfer and energy deposition with different F-SAM surface concentrations, while non-linear changes occur for ion-surface reaction suggesting strong lateral interactions between the two components. These interactions are minimized in the μ-CP system containing domains of each thiol. Energy deposition on the patterned surfaces varies non-linearly with changing F-SAM concentration which differs from the homogenously mixed system.To explore SID with a 90 collision angle, eV SID of a series of protonated peptide ions were performed in an in-line sector Time-Of-Flight (TOF) mass spectrometer. The results were compared to keV collision-induced dissociation (CID) data collected with the same instrument. Fragmentation efficiency for SID was higher than CID for those peptides. In addition to the excellent control over laboratory collision energies with SID, different amount of energy deposition can be achieved when varying surface composition, e.g. using mixed F-SAM/H-SAM.Reactive ion scattering spectrometry (RISS) results provided more in-depth knowledge of low energy ion-surface interactions that will promote usage of RISS as a novel surface characterization technique.
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MASS SPECTROMETRY DISSOCIATION STUDIES OF PROTEIN-PROTEIN AND PROTEIN-NUCLEIC ACID COMPLEXES AND 13C FLUX OF AMINO ACIDSMa, Xin 09 September 2014 (has links)
No description available.
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Novel Native Mass Spectrometry-based Fragmentation and Separation Approaches for the Interrogation of Protein ComplexesVanAernum, Zachary L. 29 September 2020 (has links)
No description available.
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Using native mass spectrometry to study the role of homo-oligomeric proteins in gene regulation by using TRAP as a model protein systemHolmquist, Melody L. 06 November 2020 (has links)
No description available.
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Incorporation of Surface Induced Dissociation into a Commercial Ion Mobility - Tandem Mass Spectrometer and Application of Mass Spectrometry Methods for Structural Analysis of Non-covalent Protein ComplexesZhou, Mowei 17 September 2013 (has links)
No description available.
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Protein Primary and Quaternary Structure Elucidation by Mass SpectrometrySong, Yang 18 September 2015 (has links)
No description available.
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