Thesis (M.S.)--University of Missouri-Columbia, 2008. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Includes bibliographical references.

Made available in DSpace on 2015-12-10T14:22:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-18. Added 1 bitstream(s) on 2015-12-10T14:28:38Z : No. of bitstreams: 1 000853170.pdf: 1637274 bytes, checksum: 8870536067ed0c31cebcec62103500bc (MD5) / O objetivo do presente trabalho foi de avaliar a uniformidade de aplicação de água utilizando o coeficiente de uniformidade de distribuição (CUD), coeficiente de uniformidade de Christiansen (CUC) e o coeficiente de uniformidade estatístico (CUE), além de determinar o coeficiente de variação de vazão (CVQ) utilizando o tubo gotejador da marca Netafim modelo Streamline 16060, após seu uso com água residuária oriunda da suinocultura. O experimento foi conduzido no Laboratório de Irrigação da UNESP-FCA, Campus de Botucatu-SP. Foi executado em uma bancada de ensaio de gotejadores, utilizando quatro segmentos do tubo gotejador com 6 metros, e escolhidos 32 emissores, onde se verificou a vazão destes emissores com as seguintes pressões: 45, 55, 65, 78 kPa, com quatro repetições para cada gotejador. Após os ensaios, o tubo gotejador foi submetido a 600 horas de trabalho com água residuária oriunda da suinocultura. Foram realizados os mesmos ensaios com o tubo gotejador novo com, 100, 200, 300, 400 e 500 horas de trabalho com água residuária. Os valores obtidos com o tubo novo e após seu uso durante 400 horas com água residuária da suinocultura para o CVQ foram classificados como bons, pela tabela da ABNT (1987). Já quando se atingiu 500 horas de trabalho as pressões 45 e 65 kPa apresentaram um CVQ de 0,5 considerado médio pela ABNT (1987) e com 600 horas de trabalho as avaliações em todas pressões foram classificadas como médio. O CUD, CUC, e o CUE, desde o ensaio inicial e até 600 horas de trabalho na bancada, foram considerados como excelentes, de acordo com Merriam & Keller (1978), apesar de alguns gotejadores ao longo do tempo apresentarem obstruções parciais. Porem com o passar do tempo o CUD e o CUE apesar de serem classificados como excelentes apresentaram um leve declínio em seus valores. Com base nos resultados obtidos, foi possível concluir que, após 600 horas... / The aim of the present work was to evaluate the application uniformity of water using the distribution uniformity coefficient (DU), Christiansen uniformity coefficient (UC) and statistical uniformity coefficient (SUC), besides determine the flow variation coefficient (FVC) using a Netafim brand 16060 Streamline model dripper tube, after its use with swine wastewater. The The experiment was done in Irrigation Laboratory of UNESP-FCA, Botucatu-SP campus. It have done in a dripper test bench using four sections of 6 meters dripper tubes, 32 emitters chosen, where the flow verified in the emitters was the following pressure: 45, 55, 65, 78kPa, with four repetitions for each dripper. After the test, the dripper tube was subordinated to 600 hours work with swine wastewater. It have done the same tests with the new dripper tube with 100, 200, 300, 400 and 500 hours of work with residual water. The obtained values with the new tube and after its use through 400 hours with swine wastewater for FVC were classified as good by ABNT chart (1987). Nevertheless, when reached 500 hours of work, the pressures 45 and 65 kPa show a FVC of 0.5 considerate medium by ABNT (1987) and with 600 hours of work, the evaluation in all pressures were classified as medium. The DU, UC and SUC, since initial test to 600 hours of work in bench were classified as excellent, according to Merriam & Keller (1978), despite of some drippers show partial obstruction through time. However, through the time, the DU and UC besides classified as excellent show a small decline in their values. Based in the obtained results, it is possible to conclude that after 600 dripper tube hours of use, the dripper keep satisfactory water application uniformity, the test showed the possibility of swine wastewater for dripper irrigation, without dripper obstruction problems in periods until 600 hours of use. For swine wastewater use in irrigation projects ...

Orientador: Carlos Ducatti / Coorientador: Juliana Célia Denadai / Banca: Dirlei Antonio Berto / Banca: Maria Márcia Pereira Sartori / Banca: Léa Silvia Sant'Ana / Banca: José Albertino Bendassolli / Resumo: Objetivou-se com este trabalho avaliar o turnover e o comportamento da incorporação do carbono nos músculos reto do abdome e semitendinoso de suínos em diferentes períodos de desenvolvimento. Foram utilizados 132 leitões (machos castrados e fêmeas) híbridos comerciais, desmamados com idade média de 21 dias, distribuídos em delineamento experimental inteiramente casualizado em quatro tratamentos com dias distintos de substituição do milho (grão de planta do ciclo fotossintético C4) das dietas por quirera de arroz (grão produzido por planta do ciclo fotossintético C3) aos 21; 42; 63 e 110 dias de idade, para mudança do sinal isotópico de carbono-13. As curvas de diluição isotópica dos músculos analisados demonstraram que os animais que tiveram a dieta C4 substituída pela dieta C3 com 42 dias de idade, atingiram o patamar de equilíbrio isotópico de forma mais evidente que os suínos que receberam dieta C3 a partir dos 21 ou 63 dias de idade. Amostras de músculos são boas escolhas para refletir mudanças na dieta de suínos por um período mais longo, apresentando grande potencial de aplicação prática para a rastreabilidade de subprodutos de origem animal na alimentação de suínos / Abstract: The objective of this study was to evaluate the behavior of the turnover and incorporation of carbon in the rectus abdomen and semitendinous muscles of swines at different periods of development. We used 132 commercial hybrids (barrows and females), weaned at an average age of 21 days, distributed in a completely randomized design with four treatments on different days of substitution of corn (grain plant the C4 photosynthetic cycle) diets by broken rice (grain produced per plant photosynthetic cycle C3) at 21, 42, 63 and 110 days of age to change the signal isotope carbon-13. The isotopic dilution curves of the muscles analyzed showed that animals that had the C4 diet replaced by C3 with 42 days of age, reached the new level of equilibrium isotopic more evident that swines fed the diet C3 from 21 or 63 days of age.Samples of muscles are good choicesto reflect changes in the diet of pigs for a longer period, showing great potential for practical application for the traceability of animal by-products in feed for pigs / Doutor

Made available in DSpace on 2014-06-11T19:33:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-11-13Bitstream added on 2014-06-13T19:23:41Z : No. of bitstreams: 1 augusto_rmn_dr_botfmvz.pdf: 421343 bytes, checksum: f2a561d21945609a75e626fe0b807148 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Universidade Estadual Paulista (UNESP) / Objetivou-se com este trabalho avaliar o turnover e o comportamento da incorporação do carbono nos músculos reto do abdome e semitendinoso de suínos em diferentes períodos de desenvolvimento. Foram utilizados 132 leitões (machos castrados e fêmeas) híbridos comerciais, desmamados com idade média de 21 dias, distribuídos em delineamento experimental inteiramente casualizado em quatro tratamentos com dias distintos de substituição do milho (grão de planta do ciclo fotossintético C4) das dietas por quirera de arroz (grão produzido por planta do ciclo fotossintético C3) aos 21; 42; 63 e 110 dias de idade, para mudança do sinal isotópico de carbono-13. As curvas de diluição isotópica dos músculos analisados demonstraram que os animais que tiveram a dieta C4 substituída pela dieta C3 com 42 dias de idade, atingiram o patamar de equilíbrio isotópico de forma mais evidente que os suínos que receberam dieta C3 a partir dos 21 ou 63 dias de idade. Amostras de músculos são boas escolhas para refletir mudanças na dieta de suínos por um período mais longo, apresentando grande potencial de aplicação prática para a rastreabilidade de subprodutos de origem animal na alimentação de suínos / The objective of this study was to evaluate the behavior of the turnover and incorporation of carbon in the rectus abdomen and semitendinous muscles of swines at different periods of development. We used 132 commercial hybrids (barrows and females), weaned at an average age of 21 days, distributed in a completely randomized design with four treatments on different days of substitution of corn (grain plant the C4 photosynthetic cycle) diets by broken rice (grain produced per plant photosynthetic cycle C3) at 21, 42, 63 and 110 days of age to change the signal isotope carbon-13. The isotopic dilution curves of the muscles analyzed showed that animals that had the C4 diet replaced by C3 with 42 days of age, reached the new level of equilibrium isotopic more evident that swines fed the diet C3 from 21 or 63 days of age.Samples of muscles are good choicesto reflect changes in the diet of pigs for a longer period, showing great potential for practical application for the traceability of animal by-products in feed for pigs

By observing these warning criteria, surgery can be safely carried out if changes of signal amplitudes are within the threshold boundary. Future studies should aim to validate and refine the "warning criteria" for intraoperative neurophysiologic monitoring in different surgery. / During stable anesthesia, experiments were completed in 31 pigs. A decrease in SEP amplitude > 25% and / or TceMEP amplitude > 65% was associated with substantial risk of postoperative motor deficit. In addition, rapid deterioration of signal within 5 min of an event, and / or a lack of signal recovery within 30 min after the initial deterioration were also predictors of postoperative paraplegia or weakness. These findings also correlated well with radiological changes in the spinal cord. The sensitivity and specificity for TceMEP to predict adverse neurologic outcome were 100% and 90.5%, respectively. / In a porcine model of direct compression and distraction of the exposed spinal cord, we measured the perioperative changes in SEP and TceMEP. This was correlated with postoperative motor function using the modified Tarlov scale. Magnetic resonance diffusion tensor imaging of the spinal cord was also performed to assess the anatomical extent of injury three days after surgery. / The spinal cord is at risk of injury during complex operations of the spine or aorta, and may result in catastrophic long term disability. Intraoperative monitoring with somatosensory evoked potential (SEP) and transcranial electric motor evoked potential (TceMEP) are commonly performed to assess the integrity of the sensory and motor pathways, respectively. The purpose of this study was to identify the minimum changes in signal amplitudes, beyond which postoperative neurologic deficit may occur. / Liu, Quanmeng. / Adviser: Matthew Tu Chan. / Source: Dissertation Abstracts International, Volume: 73-02, Section: B, page: . / Thesis (Ph.D.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 87-103). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [201-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

Kang, Ning. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references. / Abstract also in Chinese. / Abstracts --- p.i / Abbreviations --- p.v / List of Figures and Tables --- p.vii / Contents --- p.viii / Chapter Chapter 1: --- Introduction --- p.1 / Chapter 1. --- Saphenous vein graft patency after coronary artery bypass grafting --- p.1 / Chapter 2. --- Mechanism of vein graft failure and therapeutic strategies --- p.8 / Chapter 2.1 --- Mechanism --- p.15 / Chapter 2.2 --- Therapeutic strategies --- p.18 / Chapter 3. --- Summary --- p.22 / Chapter 4. --- References --- p.23 / Chapter Chapter 2: --- Animal model and laboratory investigations --- p.34 / Chapter 1. --- Surgical procedure --- p.35 / Chapter 2. --- Postoperative management --- p.37 / Chapter 3. --- Adenoviral-mediated gene transfer ex vivo for gene therapy study --- p.38 / Chapter 4. --- Laboratory investigations --- p.39 / Chapter 5. --- Statistical analysis --- p.40 / Chapter 6. --- Summary --- p.41 / Chapter 7. --- References --- p.41 / Chapter Chapter 3: --- "Impact of osteopontin expression in vein grafts on VSMC migration, proliferation, and neointimal formation" --- p.42 / Chapter 1. --- Introduction --- p.42 / Chapter 2. --- Methods and materials --- p.43 / Chapter 3. --- Results --- p.43 / Chapter 4. --- Discussion --- p.49 / Chapter 5. --- Summary --- p.52 / Chapter 6. --- References --- p.53 / Chapter Chapter 4: --- Potential Role of gene therapy in prevention of vein graft failure --- p.56 / Chapter 1. --- Vectors --- p.56 / Chapter 2. --- "Reporter gene, timing and titer" --- p.57 / Chapter 3. --- Candidate genes --- p.58 / Chapter 4. --- Summary --- p.64 / Chapter 5. --- References --- p.66 / Chapter Chapter 5: --- Conclusions --- p.70 / Acknowledgements --- p.72

Indiana University-Purdue University Indianapolis (IUPUI) / For years, the leading killer of Americans has been coronary artery disease (CAD), which has a strong correlation to the U.S. obesity epidemic. Obesity, along with the presence of other risk factors including hyperglycemia, hypercholesterolemia, dyslipidemia, and high blood pressure, comprise of the diagnosis of metabolic syndrome (MetS). The presentation of multiple MetS risk factors increases a patients risk for adverse cardiovascular events. CAD is a complex progressive disease. We utilized the superb model of CAD and MetS, the Ossabaw miniature swine, to investigate underlying mechanisms of CAD progression. We studied the influence of coronary epicardial adipose tissue (cEAT) and coronary smooth muscle cell (CSM) intracellular Ca2+ regulation on CAD progression. By surgical excision of cEAT from MetS Ossabaw, we observed an attenuation of CAD progression. This finding provides evidence for a link between local cEAT and CAD progression. Intracellular Ca2+ is a tightly regulated messenger in CSM that initiates contraction, translation, proliferation and migration. When regulation is lost, CSM dedifferentiate from their mature, contractile phenotype found in the healthy vascular wall to a synthetic, proliferative phenotype. Synthetic CSM are found in intimal plaque of CAD patients. We investigated the changes in intracellular Ca2+ signaling in enzymatically isolated CSM from Ossabaw swine with varying stages of CAD using the fluorescent Ca2+ indicator, fura-2. This time course study revealed heightened Ca2+ signaling in early CAD followed by a significant drop off in late stage calcified plaque. Coronary artery calcification (CAC) is a result of dedifferentiation into an osteogenic CSM that secretes hydroxyapatite in the extracellular matrix. CAC is clinically detected by computed tomography (CT). Microcalcifications have been linked to plaque instability/rupture and cannot be detected by CT. We used 18F-NaF positron emission tomography (PET) to detect CAC in Ossabaw swine with early stage CAD shown by mild neointimal thickening. This study validated 18F-NaF PET as a diagnostic tool for early, molecular CAC at a stage prior to lesions detectable by CT. This is the first report showing non-invasive PET resolution of CAC and CSMC Ca2+ dysfunction at an early stage previously only characterized by invasive cellular Ca2+ imaging.