Concern over rising atmospheric CO2 due to fossil fuel combustion has intensified research into carbon-neutral energy and fuel production. Therefore, bioenergy production has expanded during the last decade, increasing demand for forest-based bioenergy feedstocks. Millions of acres of privately and industrially owned pine plantations exist across the southeastern US, representing a vast area of land that could be utilized to produce bioenergy without significant land-use change or diversion of agricultural resources from food production. Furthermore, loblolly pine (Pinus taeda L.) plantations offer the unique opportunity to utilize space between rows of planted trees to grow an herbaceous bioenergy crop, such as switchgrass (Panicum virgatum L.). This novel forest management regime has the potential to provide positive environmental and economic services, but hinges in part on impacts to soil carbon (C) and nitrogen (N) cycling, availability of belowground resources, and potential negative impacts of competition between pine and switchgrass on plant productivity. Three specific objectives were addressed in this study: 1) compare different bioenergy management regimes in regards to temporal dynamics of N cycling and availability following forest establishment (see Chapter 2); 2) determine the impact of loblolly pine and switchgrass intercropping on microbial N cycling processes (see Chapter 3); and 3) evaluate chemical and physical mechanisms of soil organic matter (SOM) stabilization and test their sensitivity to pine-switchgrass intercropping (see Chapter 4). The study site was located in the Lower Coastal Plain physiographic province in Lenoir County, North Carolina, USA (35-12'59'' N; 077-26'13'' W). Soils were mapped as Pantego (fine‐loamy, siliceous, semiactive, thermic Umbric Paleaquults) or Rains (fine‐loamy, siliceous, semiactive, thermic Typic Paleaquults) soil series, both of which are very poorly drained. However, previous site management in the late 1960’s and early 1970’s included installation of ditches to lower the water table and reduce saturation at the soil surface. Additionally, bedding of soil in rows was used to raise root systems of planted loblolly pine seedlings above the water table, increase soil aeration, and reduce competition. Space between bedded rows of pine trees was referred to as the interbed. Results from Chapter 2 showed that switchgrass significantly reduced interbed soil NH4 + and NO3 - concentrations by 39% and 60%, respectively, over the course of the timeframe (30 months) of this study. Surprisingly, in beds of the pine-switchgrass treatment significant increases in NO3 - concentration were measured from July - December 2011. From Chapter 3, gross N mineralization rates ranged from 0.18 - 4.7 µg N g -1 soil d-1 , while gross nitrification rates ranged from 0.02 - 0.47 µg N g-1 soil d-1 . At the 0-5 cm depth in switchgrass interbeds, gross N mineralization was reduced from April to November potentially reflecting microbial C limitations due to reduced soil C concentrations. At the 0-5 cm depth in beds of the pine-switchgrass treatment, gross N mineralization rates were elevated by 1.29 µg N iii g -1 soil d-1 in November and 1.02 µg N g-1 soil d-1 in February on average corresponding to a 305% and 193% increase, respectively. From Chapter 4, total C content in beds and interbeds ranged from 15 to 88 Mg C ha-1 and was reduced by 27% in beds of the pine-switchgrass treatment. Average C concentration for aggregate fractions was significantly lower in beds of the pine-switchgrass treatment at 0-5, 15- 30, and 30-45 cm depths, amounting to ~23%, ~28%, and ~34% reduction, respectively. Values of δ 13C for the >2000 µm aggregate size fraction at the 0-5 cm depth were diluted, corresponding to estimates of 13 - 25% of the >2000 µm C pool comprised of new pine-derived C. For SOM fractionated by density, elevated C concentrations were found in the occluded light fractions in both beds and interbeds of the pine-switchgrass treatment. Enriched δ13C in occluded light fractions led to estimates of 2.5 - 12.5% of this C fraction comprised of new switchgrass-derived C. In the free light fraction, new pine-derived C accounted for 15% and 9% of C at the 5-15 and 15-30 cm depth, respectively. Three overarching conclusions were generated from my research: 1) switchgrass grown between loblolly pine trees effectively utilized excess soil NH4 + and NO3 - when N availability was high following harvesting of a mature plantation proceeded by establishment of a second rotation of loblolly pine (see Chapter 2); 2) gross N mineralization rates were reduced under switchgrass during the growing season when soil C availability was low, but were elevated under switchgrass and adjacent loblolly pines when switchgrass was dormant and C availability was likely higher (see Chapter 3); and 3) SOM stabilized by physical or chemical mechanisms responded differently to pine-switchgrass intercropping, with losses in aggregate-stabilized C and gains in occluded, mineral-stabilized C. Furthermore, losses of aggregate C was associated with a significant reduction in total soil C in beds of the pine-switchgrass treatment. Results from 13C mass balance suggested incorporation of switchgrass-derived C into occluded light fractions of beds and interbeds. Finally, incorporation of new pine-derived C into the >2000 µm aggregate size fraction and free light fraction indicate pine inputs of particulate organic matter into these SOM fractions in beds of the pine-switchgrass treatment (see Chapter 4). I hypothesize that loblolly pines have increased root growth in beds in response to competition with switchgrass for N in the interbed, thereby alleviating seasonal microbial C limitations and stimulating microbial N cycling processes and increasing plant-available N. Overall, this research suggests that soil C and N cycling in pine plantations is altered by intercropping of pine and switchgrass. Through a mechanistic understanding of how C and N are cycled in forests and the impact of various forest management regimes on soil C and N cycling, effective management strategies can be implemented to utilize forests for intensive biomass production while limiting loss of soil C and N, and in some cases even enhancing soil C and N retention. Future research initiatives should seek to unravel the complex belowground interactions between roots of different plant species and soil microbial communities competing for limiting resources. Understanding how these interactions drive soil C storage, N cycling and availability, and forest productivity will ultimately improve resource utilization in these managed ecosystems as well as our basic understanding of how natural and managed ecosystems function. / Ph. D.

Secondary generation biofuels such as cellulosic biofuels rely on large portions of cellulosic bioresources, which may include forests, perennial grasses, wood and agricultural residues. Switchgrass is one promising feedstock for biofuel production. In the present study, thesis work focused on the chemical and structural profiles and hydrothermal pretreatment of switchgrass. Four populations of switchgrass were investigated for their chemical properties among populations and morphological portions, including the compositions of lignin and carbohydrates, extractives content, higher heating value (HHV), and syringyl:guaiacyl (S:G) ratio. The results demonstrate similar chemical profiles and lignin structure among the four populations of switchgrass. Morphological fractions of switchgrass including leaves, internodes, and nodes differ significantly in chemical profiles and S:G ratios of lignin. The structure of isolated cellulose from switchgrass SW9 is similar between leaves and internodes. The structure of isolated lignin from leaves and internodes of switchgrass SW9 differs in S:G ratio and molecular weight. Hydrothermal pretreatment of leaves and internodes indicates that a similar chemical composition and chemical structure for pretreated leaves and internodes. The degree of polymerization (DP) for cellulose of the pretreated internodes is 23.4% greater than that of the pretreated leaves. The accessibility of pretreated leaves measured by Simons' Staining technique is greater than that of pretreated internodes. Pretreated leaves have a 32.5-33.8% greater cellulose-to-glucose conversion yield than do pretreated internodes.

Switchgrass is a C4 perennial grass that is currently being developed for use as a second generation lignocellulosic biofuel crop. For switchgrass to be fully utilized as a bioenergy crop, large-scale plantings of elite switchgrass germplasm, possibly in monoculture, are likely to occur. This practice may increase the selection pressure on plant pathogens, such as switchgrass rust, which could result in devastating disease epidemics. The identification and deployment of quantitative trait loci (QTLs) and major plant disease resistance genes (R) in switchgrass breeding programs could offer broad spectrum and durable disease resistance in commercial switchgrass cultivars. 'Alamo', a lowland cultivar, is generally resistant to switchgrass rust whereas 'Dacotah', an upland cultivar, is highly susceptible. I hypothesized that major R genes and/or QTLs were contributing to the differences in disease phenotypes of these two cultivars. In this dissertation, bioinformatics and molecular biology approaches were employed to dissect the genetic mechanisms underlying switchgrass rust disease resistance. Novel pseudo-F2 mapping populations were created from a cross derived from 'Alamo' and 'Dacotah'. RNA-sequencing of the pseudo-F2 progenies of 'Alamo' x 'Dacotah' was used to construct a genetic linkage map and to identify potential QTLs correlating with disease resistance. In addition, a homology-based computational method was used to identify 1,011 potential NB-LRR R genes in the switchgrass genome (v 1.1). These potential R genes were characterized for polymorphism and expression differences between 'Alamo' and 'Dacotah'. Moreover, I found that some NB-LRR genes are developmentally regulated in switchgrass. One of the major objectives of switchgrass breeding programs is to develop cultivars with improved feedstock quality; however, changes in the components of the plant cell wall may affect disease resistance. I hypothesized that genetically modified switchgrass plants with altered cell wall components will respond differently than the wild-type to switchgrass rust. Transgenic switchgrass plants overexpressing AtSHN3, a transcription factor with known functions in epicuticular wax accumulation and cell wall deposition, were created. I found that AtSHN3-overexpressing transgenic switchgrass lines were more susceptible than wild-type plants in their response to switchgrass rust. Overall, the results of this dissertation provide a platform for elucidating the molecular mechanisms underlying resistance of switchgrass to switchgrass rust. These findings will help breeders create switchgrass cultivars with improved disease resistance, and will ultimately allow switchgrass to be used for sustainable biomass production. / Ph. D.

The temperature and moisture content of biomass feedstocks both play a critical role in minimizing storage and transportation costs, achieving effective bioconversion, and developing relevant postharvest quality models. Hence, this study characterizes the heat and mass transfer occurring within baled switchgrass through the development of a mathematical model describing the relevant thermal and physical properties of this specific substrate. This mathematical model accounts for the effect of internal heat generation and temperature-induced free convection within the material in order to improve prediction accuracy. Inclusion of these terms is considered novel in terms of similar biomass models. Two disparate length scales, characterizing both the overall bale structure (global domain) and the individual stems (local domain), are considered with different physical processes occurring on each scale. Material and fluid properties were based on the results of hydraulic conductivity experiments, moisture measurements and thermal analyses that were performed using the constant head method, TDR-based sensors and dual thermal probes, respectively. The unique contributions made by each of these components are also discussed in terms of their particular application within various storage and bioconversion operations. Model validation was performed with rectangular bales of switchgrass (102 x 46 x 36 cm3) stored in an environmental chamber with and without partial insulation to control directional heat transfer. Bale temperatures generally exhibited the same trend as ambient air; although initial periods of microbial growth and heat generation were observed. Moisture content uniformly declined during storage, thereby contributing to minimal heat generation in the latter phases of storage. The mathematical model agreed closely with experimental data for low moisture content levels in terms of describing the temperature and moisture distribution within the material. The inclusion of internal heat generation was found to be necessary for improving the prediction accuracy of the model; particularly in the initial stage of storage. However, the effects of natural convection exhibited minimal contribution to the heat transfer as conduction was observed as the predominate mechanism occurring throughout storage. The results of this study and the newly developed model are expected to enable the maintenance of baled biomass quality during storage and/or high-solids bioconversion.

<div>Switchgrass (Panicum virgatum L.) is an important warm-season perennial grass in livestock systems and has been extensively researched as an herbaceous energy crop. Objectives of this series of studies were to compare morphological development, compositional quality, crop growth, and yield of a recently developed biofuel cultivar ‘Liberty’ to an improved forage cultivar ‘Shawnee’ in multiple Indiana environments. Pure stands of each cultivar were sampled in the field at Trafalgar and Roann, Indiana in 2016. In 2017, samples were collected at Trafalgar, Roann, and Lafayette, Indiana. Samples were collected weekly during the early season and every other week in the late season with development determined by use of the Mean Stage Count (MSC) and Mean Stage Weight (MSW) system.</div><div>In the morphological development study, MSC and MSW were linearly related to both GDD and DOY for both years. ‘Liberty’ growth lagged behind ‘Shawnee’ throughout the whole growing season by approximately seven days. Prediction equations for MSC and MSW were developed based on accumulated GDD and DOY for Trafalgar and Roann in 2017. The prediction equations for MSC as predicted by GDD explained from 84 to 93 percent of the variation in MSC across locations for ‘Shawnee’ and between 90 to 94 percent of the variation for ‘Liberty’. For MSW, ‘Shawnee’ and ‘Liberty’ prediction equations explained from 84 to 93 percent and 90 to 95 percent of the variation as predicted by GDD across locations, respectively.</div><div><br></div><div>In the compositional quality study, samples from every other sampling date were ground and analyzed using near-infrared reflectance spectroscopy (NIRS). Increasing nitrogen fertilizer caused a higher nitrogen concentration at a given MSC. The 0 kg N ha-1 fertilizer rate dropped below 10 mg g-1 nitrogen by MSC 2.2, whereas the 134 kg N ha-1 fertilizer rate had greater than 10 mg g-1 until MSC 2.7. ‘Liberty’ had increased Neutral Detergent Fiber (NDF) concentration as compared to ‘Shawnee’. For whole-plant samples, ‘Liberty’ averaged 727 mg g-1 NDF as compared to ‘Shawnee’ which averaged 718 mg g-1. ‘Liberty’ had 18 mg g-1 higher acid detergent fiber (ADF), on average, as compared to ‘Shawnee’. Acid Detergent Lignin (ADL) was not different among nitrogen fertilizer treatments. Stem-plus-sheath material accounted for a higher percentage of NDF, ADF, and ADL, in whole-plants as MSC increased, as compared to leaf blades. ‘Shawnee’ had higher IVDMD as compared to ‘Liberty’ and the biggest differences occurred around MSC 2.9. At MSC 2.9, ‘Shawnee’ whole-plant IVDMD was 448 mg g-1 and ‘Liberty’ whole-plant IVDMD was 430 mg g-1. Whole-plant ash concentration decreased as MSC increased.<br></div><div><br></div><div>For the study that evaluated crop growth and yield, differences in grams m-2, mass tiller-1, and tiller number per unit area were analyzed in response to growing degree days (GDD) and day of year (DOY). Number of tillers had a negative linear response to GDD and DOY for both years, whereas, mass tiller-1 had a positive linear response to GDD and DOY for both years. Grams m-2 responded quadratically to GDD and DOY. Generally, ‘Liberty’ had 20 percent higher mass tiller-1 and lower number of tillers per m-2 at the end of the season as compared to ‘Shawnee.’ Addition of nitrogen fertilizer generally increased mass tiller-1 and grams m-2. Roann, the northern most site, also had highest tiller numbers at the beginning of the season and decreased faster than at the central Indiana sites. ‘Liberty’ yielded 8.8 percent higher than ‘Shawnee’ across locations, nitrogen rates, and sampling years. Addition of nitrogen fertilizer did not conclusively increase yield. Grams m-2, mass tiller-1, and tillers per sample area helped explain some yield differences. For example, ‘Liberty’ had increased yield as compared to ‘Shawnee’, and ‘Liberty’ also had higher mass tiller-1 with no differences in tiller number between cultivars. While additions of nitrogen fertilizer increased grams per tiller, yield was not significantly increased with added nitrogen fertilizer. Therefore, these measures should not stand alone as a predictor of yield differences between cultivars. Switchgrass is a bunchgrass and has inherent difference in numbers of plant and tillers per plant within a plot, which may not be truly represented by one crop growth parameter alone.</div><div><br></div><div>This study confirms that switchgrass has great potential as a forage and biofuel crop in Indiana with low nitrogen fertilizer requirements and high yield. Understanding how switchgrass morphological development, compositional quality, growth, and yield responds in Indiana environments across locations, years, and nitrogen rates will help guide the future switchgrass management decisions of producers and researchers.</div>

Polychlorinated biphenyls (PCBs) are carcinogenic, persistent, and bioaccumulative contaminants that pose risks to human and environmental health. PCB biodegradation by indigenous microbial communities could be a cost-effective and an environmental-friendly bioremediation strategy for in situ PCB removal. A comprehensive understanding of the microbial PCB degradation at the contaminated site is required for the acceptance and optimization of using microbial PCB degradation as the site clean-up strategy. This thesis describes investigations of the aerobic and anaerobic microbial degradation of PCBs under both field and laboratory conditions. The microbial PCB degradation potential in sediments from Indiana Harbor and Ship Canal (IHSC), a site that was historically contaminated by PCBs, was explored by analyzing the PCB congener distributions and microbial communities in two core sediment samples. PCB congener analysis suggested the possibility of in situ dechlorination in deep sediments. Molecular analysis of biomarker genes revealed the potential of both aerobic and anaerobic PCB degradation in sediments. Microbial communities were characterized by the combination use of terminal restriction fragment length polymorphism (T-RFLP), clone library, and pyrosequencing. These methods elucidated the dominant role of Proteobacteria, especially Acidovorax and Acinetobacter in sediments. To improve the microbial PCB degradation, phytoremediation with switchgrass (Panicum vigratum) was employed under laboratory conditions. Congener analysis showed that both phytoextraction and microbial PCB degradation contributed to the enhanced PCB removal in the presence of switchgrass. Bioaugmentation with Burkholderia xenovorans LB400 was performed to further promote aerobic PCB degradation. The presence of LB400 was associated with improved degradation of PCB 52, but not PCB77 or PCB 153. Increased abundance of the biphenyl dioxygenase gene, which is indicative of aerobic PCB degradation, and its transcript were observed after bioaugmentation, suggesting active aerobic PCB degradation. To promote the anaerobic PCB degradation, redox cycling (alternating flooding and non-flooding) was performed. Redox cycling was found to improve the removal of PCB 153 in unplanted soils and to increase the dechlorinating Chloroflexi population. Characterization of the microbial community by T-RFLP and clone library revealed that Proteobacteria and Acidobacteria were dominant. Species that contain dechlorination potential were identified, including Geobacter and Clostridium, suggesting that their possible role in PCB dechlorination. The research described in this thesis provides scientific knowledge and evidence for the feasibility of employing bioremediation including natural attenuation, phytoremediation, and bioaugmentation to clean up PCB contamination. Such information will be critical in selecting and optimizing remediation strategies for PCB contaminated sites.

Concerns regarding energy security, resource sustainability, and environmental protection have heightened interests in renewable fuels and sparked the research and development of ethanol as a transportation fuel. This study examines consumers’ willingness to pay for ethanol from various potential feedstocks; corn, switchgrass and wood wastes. Data was collected via a survey of fuel consumers across the United States in 2009. Results show that consumers have a preference for E85 (a fuel blend with 85 percent ethanol and 15 percent gasoline) from corn, switchgrass and wood wastes compared to E0 (gasoline) and a preference for E85 from switchgrass and wood wastes, but not corn when compared to E10 (10 percent ethanol and 90 percent gasoline). Also, consumers have a preference for E85 compared to E10 but not compared to E0. Mean WTP for E85 was insignificant across all models, but significant for all other product attributes; percentage of fuel imported, percentage of greenhouse gas emissions reduced, and the proximity of fuel in driving distance. This suggests a WTP for a combination of fuel attributes associated with ethanol rather than just for E85. Results suggest that price and proximity of the fuel have a greater impact on fuel selection than percentage of the fuel imported and reductions in greenhouse gas emissions. Republicans had a positive WTP for E85 compared to E10 and a negative WTP for E85 compared to E0 regardless of feedstock, which may suggest that Republicans actually have no preference for E85; however, these findings may also suggest that Republicans view E85 as a voluntary “policy” whereas E10 is an example of government intrusion in the free market. Thus, they may ultimately have preferences over the manner in which the blend is being introduced to the market. Across all models, those undecided in political affiliation, those previously familiar with ethanol, and those who prefer to devote U.S. farmland to food instead of fuel generally exhibited a lower WTP for E85 while Westerners, those worried about the environment, and those believe that reducing dependence on foreign oil is more important than environmental protection generally had a greater WTP for E85.

Biofuels have been widely recognized as a potential renewable energy source, and the United States’ government has been interested in producing ethanol from lignocellulosic biomass such as switchgrass. To evaluate whether lignocellulosic biomass based biofuels production is economically feasible, this paper estimated the capital investment outlays, operation costs, and net present value for investment in alternative switchgrass feedstock supply chain configurations in East Tennessee a 25 million gallon per year ethanol biorefinery. Two scenarios are analyzed in the study. The conventional hay harvest scenario includes the production, harvest, storage and transportation of biomass feedstocks from the fields to the biorefinery. The preprocessing scenario added preprocessing facilities into the biomass supply chain. According to various harvest, storage, preprocessing, and harvest equipment options, analysis and comparisons were made among different systems. The capital budgeting model developed in this study generated the optimal feedstock supply chain configurations to determine the largest net present value of cash flow from investment. Results of this study shown that with the Biomass Crop Assistance Program (BCAP) incentives, a round bale system using feedstock stored without tarp on pallets using custom hired equipment had the largest positive net present value. By comparison, if all the harvest equipment is purchased rather than custom hired, the stretch wrap baler preprocessing systems, using switchgrass harvested by a chopper with rotary cutter-header, was found to have a cost advantage over conventional hay harvest logistic systems (large round bale and large square bale systems) and pellet preprocessing systems. Assuming most likely values for switchgrass price and production costs, none of the feed stock supply chain configurations evaluated in this study produced a positive net present value when BCAP subsidies were assumed to not be available. However, without the BCAP incentives and based on combination of optimistic assumption, the round bale system using feedstock stored without tarp on pallets using custom hired equipment still has the largest positive net present value. Without the BCAP incentives, no feedstock supply chain configuration using purchased rather than custom hired equipment generated a positive net present value.

The goal of this study is to evaluate the introduction of cellulosic ethanol conversion plants using switchgrass as the feedstock and how it impacts the economies of two Tennessee regions. Switchgrass feedstock production, storage, and transportation costs are estimated for one plant in West Tennessee and one plant in East Tennessee. In each region, the location for a cellulosic ethanol conversion plant and the acreage required to meet a 61.8 million-gallon/year capacity are specified. The costs associated with switchgrass production and cellulosic ethanol conversion are then entered into IMPLAN to estimate the economic impacts of one cellulosic ethanol plant in each region. The key findings of the study for West Tennessee are as follows. The investment impacts of switchgrass farming are $100.4 million more in total industry output, an increase of 914 jobs, and $46.9 million more in total valued added. Investment impacts of a cellulosic ethanol conversion plant include $121.3 million increase in total industry output, an additional 653 jobs, and a $47.7 million increase in total valued added. Year-to-year operations of switchgrass farming and cellulosic ethanol conversion increase the region’s total industry output by $57.1 million, increase jobs by 296, and increase total value added by $26 million. In East Tennessee the investment impacts of switchgrass farming are $118.3 million increase in total industry output, 949 jobs created, and a $66.4 million increase in total valued added. Investment impacts of a cellulosic ethanol conversion plant include a $116.9 million increase in total industry output, an additional 765 jobs, and a $48.3 million increase in total valued added. Year-to-year operations of switchgrass farming and cellulosic ethanol conversion increase the region’s total industry output by $80.4 million, jobs by 396, and the total value added by $39.2 million.

Switchgrass, Panicum virgatum, is being developed as an economically and ecologically sustainable biomass crop. Nitrogen is considered one of the most limiting inputs of switchgrass. Alternatives to synthetic nitrogen fertilization may be nitrogen-fixing legumes interseeded into switchgrass. The objectives of this research were: (1) develop efficient legume management strategies for switchgrass production systems, (2) evaluate and identify cool and warm-season legumes that can be grown compatibly with switchgrass, (3) determine whether switchgrass yields are increased by legume N-fixation, and (4) determine N-fixation of common (Vicia sativa) and hairy vetch (Vicia villosa). This study examined the establishment and persistence of ten different legume species in ‘Alamo’, a lowland variety of switchgrass in two switchgrass production systems: a one-cut biomass harvest and a two-cut forage/biomass harvest. Cool-season legumes were alfalfa (Medicago sativa), arrowleaf clover (Trifolium vesiculosum), common vetch, crown vetch (Securigera varia), red clover (Trifolium pretense), hairy vetch, and crimson clover (Trifolium incarnatum). Warm-season legumes were Illinois bundle flower (Desmanthus illinoensis), trailing wild bean (Strophostyles helvula), and partridge pea (Chamaechrista fasciculata). Red clover showed the highest plant densities and increase in switchgrass yields when interseeded into existing switchgrass stands in both harvest systems. Crude protein levels were highest in the 135 kg N ha-1 treatment in the forage cut of the two-cut harvest system. Arrowleaf clover, crimson clover, and red clover had high stand densities with annual reseeding. A combination of cool-season legumes, crimson clover and common vetch, in combination with warm-season partridge pea, were established in existing switchgrass stands after one year. Common vetch was evaluated for its nitrogen fixing capacity, seed germination, establishment, and effects on yield of switchgrass. Scarification by sulfuric acid had higher seed germination than other scarification treatments, except 100 grit sandpaper treatment for one minute at 0.7 kg of pressure. Common and hairy vetch nitrogen contributions were 59.3 and 43.3 kg N ha-1 respectively at seeding rates of 6.7 kg PLS ha-1. Switchgrass yields can increase with common and hairy vetch seeding rates of 7.6 and 10.4 kg PLS ha-1 to achieve 67 kg N ha-1, the recommended rate of N-fertilization for switchgrass stands.