京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第19045号 / 農博第2123号 / 新制||農||1032(附属図書館) / 学位論文||H27||N4927(農学部図書室) / 31996 / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 縄田 栄治, 教授 舟川 晋也, 准教授 赤松 美紀 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(医科学) / 甲第21689号 / 医科博第93号 / 新制||医||1036(附属図書館) / 京都大学大学院医学研究科医科学専攻 / (主査)教授 斎藤 通紀, 教授 中川 一路, 教授 竹内 理 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

No description available.

No description available.

In recent years, there has been a rise in the use of New Psychoactive Substances (NPS) that mimic the effects of controlled drugs and licensed medicines, which have become a significant public health concern globally. Synthetic cannabinoids, a rapidly expanding category of NPS, with much higher potency and binding affinity to the cannabinoid receptors than ∆9-tetrahydrocannabinol (THC), have led to serious psychiatric complications and other adverse effects. This project aims to synthesize and analyze four potential metabolites of a synthetic cannabinoid, ADB-5´Br-BUTINACA, to determine if the synthesized metabolites correspond to those produced in human hepatocytes. The metabolites were synthesized by alkylation, amide coupling, and hydrolysis/TFA treatment and analyzed using high-performance liquid chromatography-mass spectrometry (HPLC-MS) and nuclear magnetic resonance (NMR). The reaction strategy combined all three steps into a single process, making it significantly easier to carry out and not requiring much expertise. The results suggest that the synthetic approach used in this project was successful in generating the desired potential metabolites, with overall yields varying from 27.5 % to 57.6 % and high purities ranging from 95.6 % to 99.3 %. However, the overall yields were lower than expected due to product loss during the transfer of the solution mixture, the possibility of side reactions, and incomplete conversion. Further optimization of reaction conditions may be necessary to improve the yield of the synthesized metabolites.

The objectives of this research are to support the development of state-of-the-art methods using remotely sensed data to detect slides or anomalies in an efficient and cost-effective manner based on the use of SAR technology. Slough or slump slides are slope failures along a levee, which leave areas of the levee vulnerable to seepage and failure during high water events. This work investigates the facility of detecting the slough slides on an earthen levee with different types of polarimetric Synthetic Aperture Radar (polSAR) imagery. The source SAR imagery is fully quad-polarimetric L-band data from the NASA Jet Propulsion Laboratory’s (JPL’s) Uninhabited Aerial Vehicle Synthetic Aperture Radar (UAVSAR). The study area encompasses a portion of the levees of the lower Mississippi river, located in Mississippi, United States. The obtained classification results reveal that the polSAR data unsupervised classification with features extraction produces more appropriate results than the unsupervised classification with no features extraction. Obviously, supervised classification methods provide better classification results compared to the unsupervised methods. The anomaly identification is good with these results and was improved with the use of a majority filter. The classification accuracy is further improved with a morphology filter. The classification accuracy is significantly improved with the use of GLCM features. The classification results obtained for all three cases (magnitude, phase, and complex data), with classification accuracies for the complex data being higher, indicate that the use of synthetic aperture radar in combination with remote sensing imagery can effectively detect anomalies or slides on an earthen levee. For all the three samples it consistently shows that the accuracies for the complex data are higher when compared to those from the magnitude and phase data alone. The tests comparing complex data features to magnitude and phase data alone, and full complex data, and use of post-processing filter, all had very high accuracy. Hence we included more test samples to validate and distinguish results.

京都大学 / 新制・課程博士 / 博士(理学) / 甲第23458号 / 理博第4752号 / 新制||理||1681(附属図書館) / 京都大学大学院理学研究科化学専攻 / (主査)教授 北川 宏, 教授 吉村 一良, 教授 竹腰 清乃理 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DGAM

No description available.

Communities are defined as the ensemble of populations that interact with each other and with the environment in a specific time and location. Community ecology studies how communities assemble, what are the patterns of diversity, abundance, and composition of species, and the processes driving these patterns. It includes four basic mechanisms for the assembly of communities: dispersal, drift, selection, and speciation, with each mechanism influencing how the communities change in a different way. Dispersal, the movement of species from one geographical location to another, plays a major role in the recolonization of barren environments and the introduction of new species to established environments. Drift (i.e., random birth and death events within a community) could, theoretically, be negligible in bacterial communities where the high population densities are expected to buffer its effect. Conversely, horizontal gene transfer can be a strong selective force, as horizontally transferred genetic material is a source of functional traits that may provide selective advantages to the recipient cells, especially in environments where strong selection pressure occurs. In my Ph.D. thesis, I aim to examine these three contrasting mechanisms in controlled, simplified bacterial communities that are designed and studied through a synthetic ecology approach. I found that even at low dispersal rates, the species abundance of planktonic bacterial communities can be homogenized by migration. This homogenization can occur even when there are strong variable selection forces interacting in each environment. I also found strong evidence on the importance of stochasticity in communities. Drift can decrease the community similarity by up to 6.3%, and increases the probabilities that species become extinct, especially in the case of rare taxa. In contrast, I found that naturally competent bacteria are favored to uptake more DNA in communities that are highly productive and phylogenetically diverse. This pattern is explained by a potential higher availability of naked DNA for naturally competent bacteria, presumably because there are more cells and the predation systems are more effective. Altogether, our findings support the theory on the importance of stochastic forces and their interaction with deterministic forces on the shaping of microbial community assembly.

Spider silk is known for its extraordinary material properties, being both very strong and extensible. Even though the fibers outperform many synthetic and natural materials, it is impractical to collect industrial amounts of silk from spiders due to their cannibalistic and venomous nature; they cannot be farmed like the commercial silk worm Bombyx mori. Thus, scientists have turned to molecular and engineering techniques to replicate the spider's silk and spinning apparatus. In the current literature there is no detailed protocol on the production of consistent synthetic fibers. To accomplish this, the fibroins and natural spinning apparatus were taken apart and analyzed in order to develop a protocol that biomimics the spider's system. The laboratory procedure, using the natural process as an example, was simplified to: protein production, purification, concentration, fiber spinning, and lastly post spin draw. Large quantities of truncated MaSp I spidroin (spider fibroin) was purified from E. coli and successfully spun into fibers using customized spinning, spooling, and stretching apparatuses. The final fiber products displayed mechanical properties that were comparable to other reported synthetic fibers, but more importantly also displayed low experimental variability between samples. The protocol developed in this study can be further used to characterize other spidroins and silk proteins, and can be further advanced to produce even better fibers with enhanced properties.