Global ETD Search

11	Modulation of Hypothalamic-pituitary-Adrenal Axis Parameters by Teneurin C-terminal Associated Peptide (TCAP)-1 De Almeida, Reuben Ricardo Joaquim 21 November 2012 (has links) Teneurin C-terminal associated peptides (TCAP) are a family of bioactive peptides found on the terminal exon of the four teneurin genes. TCAP-1 is found within brain regions that modulate the activity of corticotropin-releasing factor (CRF), which is the principal neuropeptide regulator of the hypothalamic-pituitary-adrenal (HPA) axis. TCAP-1 has suppressive effects on CRF-induced anxiety behaviours in rats. However, previous studies determined that TCAP-1 does not act directly on the CRF receptors (CRFR). Thus, I postulate that TCAP-1 may act centrally to modify elements of the HPA axis. Using an immortalized mouse hippocampal cell line, I tested the hypothesis that TCAP acts either downstream of CRFR activation, or on the regulation of the glucocorticoid receptors (GCR), which modulate CRF actions. These studies indicate that TCAP-1 represents a novel peptide in the regulation of stress related systems, which acts independently of either CRF-, or glucocorticoid- mediated signal transduction and transcription. teneurin c-terminal associated peptides corticotropin-releasing factor glucocorticoid receptor mineralocorticoid receptor hippocampus neuroendocrinology hypothalamic-pituitary-adrenal axis stress 0317 0379 0307
12	A Novel Pathway for Enhanced Metabolic Capacities Underlies the Neuroprotective Actions of Teneurin C-Terminal Associated Peptide (TCAP)-1 Xu, Mei 27 November 2012 (has links) Teneurin C-terminal Associated Peptide (TCAP)-1 is postulated to play a critical role in cellular defense mechanisms as it is highly neuroprotective against alkalotic and hypoxic stress. Optimization of metabolic pathways is recognized as an essential survival tactic by alleviating energy deficits and meeting the demands to cope with the stressors. The aim of this research was to delineate the mechanism through which TCAP-1 confers protection. My findings show that TCAP-1 increases the overall expression of GLUT1 and enhances overall expression and membrane localization of GLUT3. With respect to metabolic parameters, chronic TCAP-1 application led to increased intracellular [ATP] with decreased intracellular [lactate], both in a dose-dependent manner, but did not alter tumourgenic glycolytic enzyme expression or mitochondrially associated apoptotic protein expression. Contrastingly, acute TCAP-1 led to decreased intracellular [ATP]. Indicative of increased cellular ATP production and physiological energy expenditure, TCAP-1 reduced serum insulin levels and subcutaneous adipocyte size in vivo. Glucose Apoptosis Stress Neuroendocrinology Protection Metabolism ATP Lactate GLUT Adipose Leptin Insulin Glycolysis Oxidation TCAP-1 TCAP Teneurin C-terminal Associated Peptide 0379 0719 0317
13	Modulation of Hypothalamic-pituitary-Adrenal Axis Parameters by Teneurin C-terminal Associated Peptide (TCAP)-1 De Almeida, Reuben Ricardo Joaquim 21 November 2012 (has links) Teneurin C-terminal associated peptides (TCAP) are a family of bioactive peptides found on the terminal exon of the four teneurin genes. TCAP-1 is found within brain regions that modulate the activity of corticotropin-releasing factor (CRF), which is the principal neuropeptide regulator of the hypothalamic-pituitary-adrenal (HPA) axis. TCAP-1 has suppressive effects on CRF-induced anxiety behaviours in rats. However, previous studies determined that TCAP-1 does not act directly on the CRF receptors (CRFR). Thus, I postulate that TCAP-1 may act centrally to modify elements of the HPA axis. Using an immortalized mouse hippocampal cell line, I tested the hypothesis that TCAP acts either downstream of CRFR activation, or on the regulation of the glucocorticoid receptors (GCR), which modulate CRF actions. These studies indicate that TCAP-1 represents a novel peptide in the regulation of stress related systems, which acts independently of either CRF-, or glucocorticoid- mediated signal transduction and transcription. teneurin c-terminal associated peptides corticotropin-releasing factor glucocorticoid receptor mineralocorticoid receptor hippocampus neuroendocrinology hypothalamic-pituitary-adrenal axis stress 0317 0379 0307
14	A Novel Pathway for Enhanced Metabolic Capacities Underlies the Neuroprotective Actions of Teneurin C-Terminal Associated Peptide (TCAP)-1 Xu, Mei 27 November 2012 (has links) Teneurin C-terminal Associated Peptide (TCAP)-1 is postulated to play a critical role in cellular defense mechanisms as it is highly neuroprotective against alkalotic and hypoxic stress. Optimization of metabolic pathways is recognized as an essential survival tactic by alleviating energy deficits and meeting the demands to cope with the stressors. The aim of this research was to delineate the mechanism through which TCAP-1 confers protection. My findings show that TCAP-1 increases the overall expression of GLUT1 and enhances overall expression and membrane localization of GLUT3. With respect to metabolic parameters, chronic TCAP-1 application led to increased intracellular [ATP] with decreased intracellular [lactate], both in a dose-dependent manner, but did not alter tumourgenic glycolytic enzyme expression or mitochondrially associated apoptotic protein expression. Contrastingly, acute TCAP-1 led to decreased intracellular [ATP]. Indicative of increased cellular ATP production and physiological energy expenditure, TCAP-1 reduced serum insulin levels and subcutaneous adipocyte size in vivo. Glucose Apoptosis Stress Neuroendocrinology Protection Metabolism ATP Lactate GLUT Adipose Leptin Insulin Glycolysis Oxidation TCAP-1 TCAP Teneurin C-terminal Associated Peptide 0379 0719 0317

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