Barley yellow dwarf virus (BYDV), an aphid transmitted luteovirus, is the most widespread and economically damaging virus of cereal crops. The work in this thesis aims to characterise the basis of the naturally occurring resistance to BYDV in cereals in three ways: Firstly, by facilitating the isolation of the Yd2 gene for BYDV resistance from barley by a map-based approach. Secondly, by determining if a BYDV resistance gene in rice is orthologous to Yd2. Thirdly, by establishing if other BYDV resistance genes in non- Ethiopian barleys are allelic to Yd2. It is hoped that the information generated in this study will ultimately assist in the production of BYDV resistant cereal cultivars. A detailed genetic map of the Yd2 region of barley chromosome 3 was constructed, containing 19 RFLP loci, the centromere and the Yd2 gene. Yd2 mapped on the long arm, 0.5 cM from the centromere, and in the mapping population of 106 F2 individuals, perfectly cosegregated with the RFLP loci XYlp, and Xwg889. This map represents the first stage in a project to isolate the Yd2 gene by a map-based approach. The isolation of Yd2 could help to elucidate the molecular mechanism of the Yd2-mediated BYDV resistance, and may allow the production of BYDV resistant cereals by genetic transformation. The RFLP markers mapped closest to Yd2 could also be useful in barley breeding, by enabling selection for both the presence of Yd2 and the absence of agronomically undesirable traits known to be closely linked to Yd2. Genetically Directed Representational Difference Analysis (GDRDA) is a technique based on subtractive hybridisation, which can be used to identify RFLP markers closely linked to a gene of interest. Two GDRDA experiments were performed with the intention of generating additional RFLP markers close to Yd2. However, the first experiment yielded RFLP probes that were not derived from the barley genome, while the second experiment yielded probes that detected repetitive sequences. It was concluded that GDRDA is of limited use in generating further markers close to Yd2. To isolate the Yd2 gene by a map-based approach, a much larger mapping population will need to be analysed to genetically resolve markers tightly linked to Yd2. If the two morphological markers uzu dwarf and white stripe,,j flank Yd2, then they could assist in this task by enabling the visual identification of F2 seedlings resulting from recombination close to Yd2. However, in this study, both morphological markers were found to be located distal to Yd2. Therefore, these two morphological markers can not be used together to facilitate high resolution genetic mapping of the Yd2 locus. It may be possible to use large-insert genomic DNA clones from the relatively small genome of rice to generate further RFLP markers close to the Yd2 gene in barley, provided that the order of orthologous sequences in barley and rice is conserved close to the Yd2 locus. To assess the feasibility of this approach, RFLP probes used to identify loci close to Yd2 were mapped in rice using a segregating rice F2 population. Five of the RFLP loci mapped together and in the same order as RFLP loci mapped close to Yd2 in barley using the same probes. By comparing the location of RFLPs mapped by other researchers in rice using probes mapped close to Yd2, the region of conserved linkage between rice and the Yd2 region was tentatively identified as the central portion of rice chromosome 1. The collinearity shown by orthologous sequences in barley and rice indicated that it may indeed be possible to use rice to assist in generating RFLP markers close to Yd2. Of all the cereals, rice is the most amenable to map-based gene isolation, due to its small genome, well developed physical and genetic maps, and its ability to be genetically transformed with high efficiency. If a BYDV resistance gene that is orthologous to Yd2 could be identified in rice, this gene could be isolated with relative ease, and then used to identify barley cDNA clones corresponding to Yd2 gene by virtue of the sequence homology expected between these genes. To test if a BYDV resistance gene from an Italian rice line is orthologous to Yd2, recombinant-inbred rice lines previously characterised for this gene were analysed using probes mapped close to Yd2 in barley. No genetic linkage was detected between the RFLP loci and the BYDV resistance gene, indicating that the gene is unlikely to be orthologous to Yd2. BYDV resistance alleles at the Yd2 locus which are of a non-Ethiopian origin may show interesting differences to Ethiopian Yd2 resistance alleles. To identify barleys which may contain resistance alleles of Yd2, ten BYDV resistant barleys not known to contain Yd2 were assessed for their resistance to the PAVadel isolate of BYDV in the glasshouse. CI 1179, Rojo, Perry, Hannchen, Post and CI 4228 were found to be the most resistant under these conditions, and were analysed further. If the resistance from these barleys is controlled by alleles of Yd2, RFLP markers close to Yd2 will be expected to cosegregate with the resistance in F2 families derived from crosses between these resistant barleys and the BYDV susceptible barleys Atlas and Proctor. RFLPs suitable for use in these allelism tests were identified using probes mapped close to Yd2. However, time did not permit the analysis of these F2 populations. / Thesis (Ph.D.) -- University of Adelaide, Dept. of Plant Science, 1996

Errata attached to inside front cover. / Bibliography: leaves [156-188] / vi, 155, [33] leaves, [48] leaves of plates : ill. (some col.) ; 30cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 2001

Includes bibliographical references. The thesis aims to characterise the basis of naturally occuring resistance to BYDV in cereals in three ways: i. A map-based approach to the isolation of the Yd2 gene for BYDV resistance from barley. -- ii. Determining if a BYDV resistance gene in rice is orthologous to Yd2. -- iii. Establishing if other BYDV resistance genes in non-Ethiopian barleys are allelic to Yd2.

The kinematics and white dwarf distribution have been studied for the Globular Cluster NGC 6397. The data was obtained from NASA’s Hubble Space Telescope in 2005. In particular, we used the images of a field 5’ Southeast of the core of NGC 6397 from Advanced Camera for Surveys to conduct our analyses. The first part of the study is about the kinematics of the globular cluster. Isotropy of velocity distribution and cluster rotation have been considered. As anticipated, this relaxed cluster exhibited no strong signs of anisotropy. However, there appears to be some level of rotation. The rotational motion turns out to be mu sub alpha cos(delta) = 3.88 ± 1.41 mas yr −1 and mu sub delta = −14.83 ± 0.58 mas yr −1. This result is not entirely expected and deserves further investigation in future studies. The second of the thesis is based on white dwarf populations in the globular cluster and the Galactic Bulge. As a first glance, there appears to be a lacking of white dwarfs at the age of approximately 0.6 Gyr. Further investigation reveals this to be statistically insignificant. Through this analysis, another pattern of white dwarf abundance is discovered. There appeared to be much more stars at the age between 0.9 − 2.0 Gyr. This could be a manifestation of modeling error. As the final consideration of this thesis, white dwarf candidates in the Galactic Bulge are illustrated. Approximately 10 candidates are found at the most probable location of stars in the Bulge. The analyses conducted in this thesis set stage for further development in understanding of globular clusters. In particular, the rotation analysis raises curiosity about the dynamics of NGC 6397 in the plane of the sky. Moreover, the velocity distribution analysis confirms properties and theories pertaining to globular clusters.

The short-term responses of Cassiope tetragona, a high arctic evergreen shrub, to variations in growing season climate were examined using experimental manipulations of temperature and growing season length at Alexandra Fiord, Ellesmere Island. Surface temperatures in the field were increased an average of 1-2 °C in two communities using open-top greenhouses. Growing season length was altered in a snowbed community by using manual snow manipulations to change the date of snowmelt. Growth and reproductive responses of Cassiope tetragona to these manipulations were observed over two field seasons following treatment establishment. Natural variations in vegetative and reproductive characteristics of Cassiope tetragona were also monitored in unmanipulated communities selected to represent a range of environmental conditions at the study site. Retrospective analysis of past Cassiope growth and reproduction was used to provide a record of variations in productivity spanning 25-35 years which could be related to climate records from Ellesmere Island. For the retrospective analysis, patterns of internode lengths were used to delimit sections of annual growth and chronologies of annual stem elongation, leaf number and flower number were then analyzed using methods similar to those applied to tree-ring studies. In general, the reproductive parameters of Cassiope tetragona were observed to be highly responsive to short-term variations in growing season climate, while vegetative production exhibited a much more conservative response. Flower production and rates of reproductive development were significantly stimulated by experimental warming. Retrospective analysis of flower production support field observations indicating that flower production is highly sensitive to annual variations in growing season temperatures. In contrast, shoot growth showed moderate responses to experimental warming. Records of past growth indicate that although vegetative production appears to be sensitive to annual variations in summer temperatures, the degree of responsiveness is much lower than for reproductive parameters. Net growth and reproduction were not stronly affected by natural or experimental variations in snowmelt timing, although phenology timing was significantly altered. The conservative growth response of Cassiope tetragona to short-term variations in climate is suggested to be related to constraints on plant phenology which may restrict flexibility in the period utilized by plants for aboveground growth. Preferential allocation of within-plant resources to reproductive structures during periods of ameliorated growing season climate may account for the observed strong reproductive responses to climate variations. Trade-offs betweeen growth and reproduction have important implications for predicting the long-term response of Cassiope tetragona to climate change. An understanding of within-plant allocation strategies is also important to the interpretation of past variations in growth and reproduction. Retrospective analysis of past Cassiope production is likely to be a very useful tool for investigating ecological relationships and past climate change.

This study focuses on the spatial distribution of the M31 satellite system. A new Bayesian technique for determining object distances from the Tip of their Red Giant Branch is developed and used to obtain distance probability distributions for M31and 27 of its satellite galaxies. These distances are then used to calculate the satellite positions in three dimensions. Subsequent analysis of the resulting spatial distribution reveals striking inhomogeneity, with roughly half of the satellites confined to a curiously oriented thin disk. The distribution is also markedly asymmetric, with the majority of satellites lying on the Milky Way side of M31.

The study of dwarf galaxies is important in order to better understand the physics of the young universe and how larger galaxies form and evolve. In this work we focus on Blue Compact Galaxies (BCGs) which havemuch enhanced star formation (starbursts), causing blue colours and strong emission line spectra. Investigating of the inner motions of BCGs provides a means for determining masses and understanding what triggered the current starburst. We have used the Very Large Telescope to perform challenging observations of the stellar motions in several BCGs, as seen in the near-infrared Ca-triplet absorption lines. By comparing these to the kinematics of the ionized interstellar medium, we were able to look into the role of feeback from stellar winds and supernova explosions, as well as further strengthen the notion that the merging of galaxies plays an important role. Spatially resolved spectroscopy can yield information about the 3D-structure of galaxies. We have used a Fabry-Perot interferometer to study the kinematics of the interstellar medium in two samples of galaxies, each containing about twenty objects. We find strong indications for ongoing galaxy mergers that correlate well with the strength of the star-formation activity. Furthermore, by estimating dynamical masses, BCGs are shown to be on average not dynamically supported by rotation. In addition, we have used data from the Sloan Digital Sky Survey to study the frequency of starbursts in the local universe and the connection to their descendants. We selected starbursts by the strength of emission in H-alpha, the first Balmer recombination line, and post-starbursts by the strength of absorption in H-delta. These are indicators of currently ongoing and recent, on the order of 100 Myr, star-formation, respectively. By modelling the stellar populations we derive ages and masses and can establish a link between starbursts and postbursts in a time sequence. We find that starbursts are active on a 100 Myr timescale but are rare objects in the local universe.

Errata sheet pasted on front end-paper. / Includes bibliographical references. / v, 125, [99] leaves, [3] leaves of plates : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, (1995?)

Thesis (Ph. D.)--University of Washington, 2006. / Vita. Includes bibliographical references (p. 139-145).

x, 62 leaves : ill. ; 29 cm Notes Typescript Thesis (M.S.)--University of Oregon, 1987 Includes vita and abstract Bibliography: leaves 54-62 Another copy on microfilm is located in Archives