This thesis discusses progress made towards assembling molecular building blocks in the presence of our molecular host of choice, cucurbit[8]uril (CB[8]). Our studies on the self-assembly of synthetic and biological components in water bridge overlapping conceptsand techniques drawn from the fields of synthetic organic chemistry, supramolecular self-assembly, and applied NMR techniques. Chapter 1 introduces the reader to chemical complexity, and how supramolecular chemistshave advanced in their capability of assembling more complex molecular architectures. The discussion focusses particularly on self-assembly carried out in the aqueousphase, and how, like in biology, molecular design of the building blocks become criticalin enabling non-covalent assembly to occur in this dynamic, and relatively competitiveenvironment. The cucurbit[n]uril family of molecular hosts are then introduced with anoverview of their modes of binding, and affinities towards typical guests. Finally, a practicalintroduction to NMR methods gaining prominence in supramolecular chemistry ispresented. In particular, the use of diffusion NMR, a key tool for probing the solutiondynamics of molecular assemblies, is highlighted. Chapter 2 details work carried out on the CB[8]-mediated self-assembly of supramolecularblock copolymers from polymeric, and small molecule building blocks. Here, endgroup-functionalised polymer guests were shown to assemble with small molecule ditopicguests in the presence of CB[8] to form block copolymers. Copolymers of various molecularweights were assembled, and the supramolecular complexes were studied usingsolution viscometry and diffusion NMR. This study represented the first use of diffusionNMR for probing the assembly of polymeric guests with CB[8].Chapter 3 describes the self-assembly of CB[8] with complementary ditopic guests. Highmolecular weight supramolecular polymers are known to form through the step-growthassembly of complementary ditopic building blocks. Here we sought to probe CB[8]?sability to drive supramolecular polymerisation. Solution viscometry, ESI-MS, and diffusionNMR were used to investigate the self-assembly process, which indicated that cyclicoligomers had formed. The relatively low solubility of CB[8] in water was thought to bea major limitation to polymer formation in this instance. Important observations relating to the effect of salts on the solution viscosities and stabilitiesof the complexes, are also discussed. Chapter 4 places emphasis on the synthetic methods employed towards preparing multivalentguests for CB[8] binding studies. Our synthetic guests were based on watersolubleoligomers of ethylene glycol. A bidirectional elongation route is presented foraccessing higher molecular weight, and monodisperse ethylene glycol oligomers (n = 12)in suitable purity. Chapter 5 describes the assembly of protein-polymer conjugates, and the versatility ofdiffusion NMR as a means to probe the assembly process. Here, end group-functionalisedpoly(ethylene glycol) guests were appended to bovine serum albumin (BSA) through amixed chemical ligation-self assembly protocol. The NMR studies conducted are emphasisedhere, which served to complement other characterisation methods used thatare reported elsewhere. Chapter 6 discusses ongoing work on lipid-based guests, and the resulting liposome assembliesformed. Head group-functionalised phospholipid guests, and cholesterol-basedguests were synthesised. Phospholipid guests were obtained through an enzymatic route,a novelty in our group. Dye-encapsulated liposomes were then assembled, purified, andcharacterised by fluorescence microscopy. Finally, we sought to optimise lipid formulationsto enhance liposome stability, towards conducting molecular recognition studies inthe presence of CB[8].Chapter 7 then closes the thesis with concluding remarks that summarise the describedresearch, while highlighting points of note.

This thesis is devoted to service quality management in the field of hotel services. It focuses mainly on an analysis of service quality and an evaluation of guest satisfaction in a selected five-star hotel in Prague. The purpose of this thesis is to find orientation in a system through which hotel investigates guest satisfaction and find its opportunities while generating useful data that will serve the hotel management. The theoretical part provides an outline of the basic methods of quality management and focuses on building a customer relationship. The practical part contains evaluation of standards and tools used at Boscolo Prague hotel along with an analysis of service quality and guest satisfaction. Attention is also paid to the effectiveness of newly introduced measures that should increase guest satisfaction. The final section is engaged in evaluating the outcomes of the analysis and in recommending possible improvements.

Cette recherche exploratoire s’articule autour du concept d’expérience client et de sa mémorisation dans le contexte de la restauration gastronomique, spécifiquement dans les restaurants des Grands Chefs Relais & Châteaux. Cette thèse a pour objectif de comprendre ce qui constitue une expérience de repas gastronomique via l’étude des éléments mémorisés par les clients. Un dispositif méthodologique en trois temps est mis en place : deux phases d’entretiens qualitatifs (n=65) et une phase quantitative (questionnaire administré en ligne, n=1855). Les échantillons sont structurés autour du critère de langue (francophone, anglophone et germanophone) et de la fréquence de consommation. L’analyse des données collectées permet de dégager d’une part les rubriques qui composent l’expérience mémorisée par les clients, d’autre part de mesurer l’influence de la familiarité individuelle sur l’interprétation des composantes de l’expérience de repas. Ce travail aboutit à l’élaboration d’un outil opérationnel à destination des Grands Chefs et des managers de restaurants. / This research is integrated into the guest experience concept and its memorization in the area of Grands Chefs Relais & Châteaux’ restaurants. This thesis aims to understand the constitution of a gourmet meal experience according to guests’ memories. A three-step methodological approach is set up: a double qualitative phase (interviews, n=65) and a quantitative phase (on-line questionnaire, n=1855). Samples are structured regarding languages (French, English and German) and consumption frequency. Data analysis permits to identify categories describing a meal experience as memorized by guests, and to measure the influence of individual familiarity on the interpretation of experiential components. This work leads to the elaboration of an operational tool dedicated to Grands Chefs and restaurant managers.

京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第22661号 / 工博第4745号 / 新制||工||1741(附属図書館) / 京都大学大学院工学研究科高分子化学専攻 / (主査)教授 山子 茂, 教授 田中 一生, 教授 村田 靖次郎 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM

This dissertation investigates non-covalent macromolecular chemistry using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) based techniques. The included studies reveal the impact of molecular structure on conformation and binding energetics. Supramolecules that might be too heavy to be dissociated in single collision-induced dissociation (CID) were dissociated using sustained off-resonance collision induced dissociation (SORI-CID) techniques. Relative binding energies and thresholds were evaluated for various macromolecular host-guest systems. Besides the non-covalent binding energies, conformation characterization was undertaken by a novel method to determine collision cross sectional areas using FTICR (CRAFTI, and multi-CRAFTI), initially developed by the Dearden lab. The systems chosen for further understanding of macromolecular interactions include calixarene-alkali metal complexes, cucurbit[5]uril-alkali halide complexes and cryptand-alkali metal complexes. The results were found to be consistent with expected behavior, and strongly correlated with predictions from computations. Size- and shape selectivity, as well as host-guest polarizability, are the main factors that govern the non-covalent macromolecular interactions that control complex conformation and dissociation. The results demonstrate the ability of FTICR to simultaneously determine binding energy, structure and conformation, which are the most important aspects for determination of comprehensive molecular characterization.

The subject of the diploma thesis is the design and process of the construction-technical part of the project documentation for realization of the new building of the mountain guest-house house in Karolina with an accommodation capacity of 36 people. The building is located on a slope, it is a basement, it has 2 underground floors and 3 aboveground floors. The building is roofed with a saddle roof with a concrete roofing. Vertical support structures are made from the Porotherm system. In addition to the project, an architectural study, a fire report and a thermal-technical assessment of the house are also included.

This master´s thesis deals with the design of architectural study of new facility for Sanatorium with speleotherapy for children Ostrov u Macochy. The construction area is located in the protected landscape area of Moravsky kras, near small town Ostrov u Macochy in Blansko county. The main ambition of this study is to fully use the potential of target area. Children sanatorium mainly focuses on the treatment of respiratory diseases by using speleotherapy. They use nearby cave system for these purposes. This project consists of three main buildings – accommodation for patients, sanatorium and guest house. These objects are designed as well-functioning and cooperative complex which along with pleasant surroundings will satisfy all requirements needed for operating such a facility. The urban solution allows easy access to the centre of city Ostrov u Machochy and also into therapeutic caves. The final form of this study respects all important aspects of affected area and tries to naturally integrate into surrounding nature.

The subject of this thesis is the elaboration of a complete business plan for a new guest house. The work is divided into two parts. The first part deals with theoretical solutions needed for a new business in the hotel industry. The theoretical part is followed by a practical part, which is processed in several types of analyzes designed to identify the company itself and its competition. Analysis also serves as a source of information to create a business plan of the new guest house, which will be able to succeed in competitive challenges and become a thriving enterprise.

Project four-storey guest house for 58 persons. The first floor is adapted for persons with reduced mobility, including one barrier-free room. The building has a rectangular plan, flat roof and will be built in the suburb of Mladá Boleslav.

This thesis deals with the project documentation of the accommodation facility. Guest house kapacity is 45 beds and 52 seated restaurant.The object is designed as a fourfloor with a partial basement. Basement floor will consist of storage, utility room. In the first floor is located main entrance, reception desk and restaurant with facilities. In the sekond and thirt floor are rooms for guests. On second floor has been situated room for invalids also. The building is walled with saddle roof.