In this thesis entitled ,,Analysis of motion of the bicycle when riding in a straight line”, I deal with the history of the bicycle, classifications and descriptions of the various parts of the bicycle and the analysis of accidents. The main objective is to analyze the movement of cyclists (bicycle) in a straight line in the implementation of tasks, typical for him - pedaling, showing a change in direction, or looking back for him. In conclusion evaluation, which can then be used in the processing of judicial opinions by experts.

This thesis named Analysis of the impact of fixed obstacles near roads on the course of traffic accidents deals with the diversification of fixed obstacles near the roads, brief theory of road accidents, possible consequences and shows statistics of road accidents with a fixed obstacle. The aim is to perform analysis of real traffic accidents with a fixed obstacle with serious consequences for health, life and property and with the help of a computer simulation software to analyze the same accident again, but for the condition if the fixed obstacle was removed. Finally, the consequences of accidents are compared, the analysis are evaluated and the proposed measures to eliminate the consequences are assessed.

The diploma thesis titled Interpretation of traffic accident analysis results in relation to related legal decision deals with the interpretation of legal decisions accidents with not giving right of way. The main goal of this thesis is to find an established line for assessing culpability for comparable accident situations. Before the analysis of the 230 court rulings measurement is performed to estimate the distance an speed, because it is closely connected with right of way. In the context of theoretical part is characterized by a right of way, an expert in criminal proceedings, the road traffic regulations, penalties and the factors that influence us while driving. The analytical part draws on 230 court rulings and 5 expert opinions provided by the Institute of Forensic Engineering of the Technical University in Brno. Conclusion of the analytical part an established threshold for assessing culpability for comparable accident situations is found.

In the era of the intensive operation on the communication, a question of the safety of the operation becomes one of the most important topics. This thesis deals with an analysis of the various factors, which just participate in the safety of operation on road in the Czech Republic. The thesis results from the comparative analysis of the traffic accident frequency in the allotted period and it takes an interest in how different factors (especially technical parameters of vehicles and human behaviour) influence the safety on highways.

The diploma thesis deals with the cost assessment of the rescue of vehicles over 18 tonnes of weight after a traffic accident on a motorway. It analyzes the factors which influence the cost of vehicle rescue and on their basis it sets out the methodology for calculating the unit price for one hour of work of emergency rescue vehicles.

The focus of this diploma thesis is to create a safety study which can be used as a tool to improve the safety of civil aviation. In the theoretical part of this thesis consideration is given to important organizations that are involved in creating legislation for civil aviation. Further, organizations which investigate aviation events were carefully examined and subsequently compared (ÚZPLN and BEA). The second part of this thesis consists of an analysis of events from 2014 using the ECCAIRS database from which were obtained suitable topics for the draft safety study. Finally a draft outline safety study was created and applied to study a specific event associated with aircraft L13 SE/SW Vivat.

The focus of this diploma thesis is on human as increasing factor in source of aviation events and effect of digital avionic systems on safety. Theoretical part discusses human factor and in detail describes human factor analysis HFACS and HRA. Further are discussed pros and cons of rise of digital instruments. Second part of thesis is focused on analysis of aviation events in Czech republic in category general aviation of maximum takeoff weight 2250kg in years 2004-2014 and analysis of accidents comparing aeroplanes with analog and digital instruments. Thesis also includes short analysis of video record using both types of instruments with focus on distribution of attention.

The Master´s thesis concerns with the choice of sources of risks and their evaluation with storage of explosives. It is divided into four parts. The first two parts are research ones and they deal with accesses to the choice of sources of risks with storage of explosives and statutory requirements for explosives, their storage, manipulation and transport and then with the buildings, which the explosives are stored in. In the next part the accesses to selection and evaluation of sources of risks in real storage units are chosen and compared. In the final part there is the chosen methodology used in case studies.

Current status of the environmental damage amount determination is mainly focused on the long-term consequences of negative factors, such as transport and industry emissions. Significant damages however, could be caused as a result of short-term effects, such as events with leakage of the hazardous chemicals. These situations are caused by accidents during the hazardous substances transportation, when the material is leaked as a result of faults on the vehicle or during a traffic accident. Damages that are subsequently being dealt with, in most cases reflect only the costs associated with the liquidation and remediation of the affected area. However it is important to note that since the inception of the damage until the return of the environment to its original state, if at all possible, there are many changes increasing other related costs associated with their solving. An example is the inability to use the functions that the damaged area provided. The aim of the thesis was therefore thoroughly analyze the current state of determining the amount of damage to the environment, to analyze appropriate methods for the unification process in the framework of expert activities and propose a systematic approach to improve the determination. The proposed procedure was subsequently implemented in a software tool that should be available for the needs of experts.

L’activateur tissulaire du plasminogène (tPA) est une sérine protéase initialement découverte dans le sang pour sa capacité à convertir le plasminogène en plasmine, une enzyme capable de dégrader les chaînes de fibrine des caillots sanguins. Pour cette fonction pro-fibrinolytique, le tPA est le seul traitement pharmacologique aujourd’hui utilisé dans la phase aiguë de l’accident vasculaire cérébral (AVC) de type ischémique, même s’il présente plusieurs limites. Outre son rôle dans la fibrinolyse, le tPA est aussi capable de moduler différents phénomènes physiologiques et pathologiques au sein du système nerveux central et de l’unité neurovasculaire, tels que la mémoire, l’excitotoxicité ou encore le couplage neurovasculaire comme décrit plus récemment. Plusieurs fonctions du tPA impliquent son interaction avec les récepteurs N-Methyl-D-Aspartate (NMDA), qui permet de potentialiser leur signalisation. Sur le plan structurel, deux formes du tPA ont été identifiées : une forme simple chaîne (sc-tPA) et une forme double chaîne (tc-tPA). Ces deux formes, dont les proportions peuvent varier dans la solution administrée aux patients pour la thrombolyse post-AVC ischémique, partagent certaines fonctions communes mais peuvent aussi avoir des actions différentes. Le premier objectif de nos travaux visait à mieux comprendre l’implication du tPA dans le couplage neurovasculaire, un phénomène essentiel au fonctionnement cérébral permettant aux régions en activité de bénéficier d’un apport accru en sang afin de subvenir à la demande énergétique des neurones. Dans une seconde partie, nous nous sommes intéressés aux effets des formes sc-tPA et tc-tPA utilisées lors de la thrombolyse dans un modèle murin d’AVC ischémique thromboembolique.Premièrement, nos résultats mettent en évidence la capacité du tPA vasculaire à augmenter l’hyperhémie fonctionnelle dans le cadre du couplage neurovasculaire. En effet, nous montrons chez la souris que le tPA vasculaire peut interagir avec les récepteurs NMDA présents à la surface des cellules endothéliales des artères et artérioles, et augmenter leur dilatation lors d’une activité neuronale. D’autre part, dans le cadre de l’ischémie cérébrale, nos résultats indiquent que lorsqu’ils sont utilisés pour la thrombolyse précoce, le sc-tPA et le tc-tPA ont des effets différents et parfois opposés. Le sc-tPA permet de réduire les volumes de lésion et d’améliorer la récupération fonctionnelle, alors que le tc-tPA est moins efficace pour réduire la lésion et ne diminue pas les déficits fonctionnels. De fait, nos données montrent que le tc-tPA aggrave l’altération de l’intégrité de la barrière hématoencéphalique par rapport au sc-tPA. Dans l’ensemble, ces données permettent d’améliorer les connaissances sur les mécanismes d’actions du tPA dans des phénomènes physiologiques et pathologiques importants. Nos travaux soulignent également la nécessité de prendre en compte les différences entre les formes de tPA dans l’amélioration du traitement actuel des AVC et dans l’élaboration de futures stratégies thérapeutiques impliquant cette molécule. / The tissue-type plasminogen activator (tPA) is a serine protease initially discovered in the blood for its ability to convert plasminogen into plasmin, an enzyme capable of degrading fibrin chains of blood clots. tPA is the only pharmacological treatment currently used for the acute phase of ischemic stroke, although it has several limitations. Besides its role in fibrinolysis, tPA also modulates various physiological and pathological phenomena within the central nervous system and neurovascular unit, such as memory, excitotoxicity and neurovascular coupling, which has been described recently. Several functions of tPA involve its interaction with N-Methyl-D-Aspartate (NMDA) receptors, which leads to an increase in NMDA signaling. Structurally, two forms of tPA have been identified: a single chain form (sc-tPA) and a double chain form (tc-tPA). These two forms, whose proportions vary in the solution administered for thrombolysis during ischemic stroke, share some common functions but may also differ in their therapeutic action. The first objective of our work was to better understand the implication of tPA in neurovascular coupling, which is an essential phenomenon for cerebral functioning that allows active brain regions to benefit from increased blood supply in order to meet local energy demands. In the second part of our work, we investigated the effects of sc-tPA and tc-tPA in a murine model of ischemic thromboembolic stroke.Our results establish a role for vascular tPA in increasing functional hyperemia in neurovascular coupling. We show that vascular tPA interacts with NMDA receptors present at the surface of endothelial cells of arteries and arterioles to increase their dilation during neuronal activity. In the context of cerebral ischemia, our results indicate that when administered during early thrombolysis, sc-tPA and tc-tPA have different and sometimes opposite effects. tc-tPA is less effective than sc-tPA in reducing lesion volume and protecting against functional impairment. In fact, our data show that tc-tPA worsens the integrity of the blood-brain barrier compared to sc-tPA. Overall, these data improve our knowledge of the mechanisms of action of tPA in important physiological and pathological phenomena. Our work underlines the need to take into account differences between sc-tPA and tc-tPA when trying to improve the current treatment for stroke and in the development of future therapeutic strategies involving this molecule.