OBJECTIVES: Standard blood flow rates for cardiopulmonary bypass have been assumed to be the same for awake cardiac surgery with thoracic epidural anesthesia as for general anesthesia. However, compared to general anesthesia, awake cardiac surgery with epidural anesthesia may be associated with higher oxygen consumption due to missing effect of general anesthetics. This may result in insufficient oxygen delivery and lactic acidosis when standard blood flow rates were used. The primary aim of our study was to investigate if standard blood flow rates are adequate in awake cardiac surgery. The secondary aim was to evaluate postoperative clinical outcomes of patients undergoing awake cardiac surgery. METHODS: Forty-seven patients undergoing elective on-pump cardiac surgery were assigned to receive either epidural (Group TEA, n=17), combined (Group TEA-GA, n=15) or general (Group GA, n=15) anesthesia. To monitor adequacy of standard blood flow rates, arterial lactate, acid base parameters, central venous and jugular bulb saturation were measured at six time points during in all groups. Blood flow rates were adjusted when needed. Subsequently, early and late postoperative outcome data including hospital and 3-year mortality was recorded and compared among the study groups RESULTS: No lactic acidosis has...

Introducción: La prevención significa reducir factores de riesgo, detener el avance de la enfermedad y atenuar sus consecuencias (OMS). En el 2006, no se encontró asociación entre los determinantes sociales en los estudiantes de medicina de una universidad peruana, y la promoción y prevención de la salud. Como objetivo se planteó determinar si existe asociación entre tener familiares profesionales de la salud y haberse realizado una radiografía de tórax, en el último año, en estudiantes de medicina de una universidad peruana. Material y métodos: Transversal analítico; que utilizó una ficha de recolección de datos, probada en piloto para calcular tamaño muestral. La población comprendió estudiantes de medicina de una universidad peruana. Se realizó un muestreo aleatorio simple y se empleó Stata12 para análisis. Resultados: El 26,38% de encuestados se realizó una radiografía de tórax, principalmente por chequeo general (57,89%); aquellos que no fue predominantemente por falta de interés (60,38%). No se encontró asociación significativa entre las variables estudiadas (RP: 1,3 IC 95%: 0,6-2,9). Conclusión: No existe asociación significativa entre tener familiares profesionales de la salud y realizarse una radiografía preventiva. Más del 70% de casos con ausencia de placa son por falta de interés. / Introduction: Prevention is defined as the reduction of risk factors, detention in the progression of the disease and minimizing its consequences (WHO). In 2006, no association was found between social determinants among medical students in a Peruvian university, and health promotion and prevention. The main of the study is to determine if there is an association in having healthcare professional relatives and getting chest x-ray in the last year, in medical students from a Peruvian university. Materials and method: analytical transversal, in which a data collection form was used, tested in a pilot in order to calculate the sample size. The study population was medical students from a Peruvian University. We performed a simple random sampling and used Stata12 for the analysis. Results: 26,38% students got a chest x-ray, mainly because of general medical examination (57,89%), the ones who did not were mostly because of lack of interest (60,38%). We didn`t found a significant association between the studied variables (PR:1,3 IC95%:0,6-2,9). 31,58% of the interviewed with healthcare professional relatives and the 24,53% without healthcare professional relatives got the test. Conclusions: There is no significant association between having healthcare professional relatives and getting a preventive chest x-ray. More than 70% of the cases that did not get a chest x-ray are due to the lack of interest. / Tesis

Cette thèse avait deux buts principaux : a) l'implémentation et l'amélioration d'une méthode de calcul de densité volumique du sein (VBD), et b) la proposition d'une mesure d'irradiation utilisable pour l'évaluation du risque individuel en mammographie avec une méthode pour l'estimer. La densité du sein est connue comme indicateur de risque du cancer. Une méthode de quantification objective de la VBD a été développée, à partir d'approches existantes, et améliorée. La méthode a été implémentée pour deux systèmes de mammographie. Elle repose sur l'étalonnage du système de mammographie et la chaîne d'acquisition avec des fantômes équivalents aux tissus mammaires. Une carte de densité est calculée.La contribution majeure de la thèse consiste en une nouvelle méthode de validation, applicable à tout calcul de VBD d'image de mammographie. Elle consiste à comparer les résultats aux valeurs de densité obtenues par des scanners thoraciques pour la même patiente. Cette validation a été appliquée à notre méthode de calcul et nous avons trouvé 10% d'écart moyen entre les deux méthodes, ce qui est comparable aux résultats de l'état de l'art. Pour le risque d'irradiation individuel, nous proposons de remplacer la dose glandulaire moyenne par l'énergie déposée, qui dépend de la quantité et de la distribution du tissu glandulaire, qui est le tissu à risque. L'énergie volumique déposée est calculée par simulation de Monte Carlo. Le VBD, calculé pour l'image de projection à 0° en tomosynthèse, aide à localiser le tissu glandulaire et à attribuer l'énergie déposée dans les tissus différents. Une proposition a été faite pour des fantômes géométriques, un fantôme texturé et un cas de patiente / In this research project the main goals were a) to implement a method for the computation of the volumetric breast density (VBD), and b) to propose an improved quantity for the assessment of individual radiation-induced risk, in particular during mammography, together with a method to quantify it. The breast density is known as a breast cancer risk factor. The objective quantification of the volumetric breast density was developed, based on already published methods, and improved. The method was implemented for two mammography systems. It is based on the calibration of the mammography system acquisition chain with breast equivalent phantoms and computes a breast density map. Our most important contribution resides in a new validation method applicable to any VBD computation, consisting in comparing its results with the VBD obtained from a thorax CT examination for the same patient. This validation method was applied to our VBD computation. We found an average deviation between mammography and CT of less than 10%. Our results are comparable to the state-of-the-art results for other validation methods. For the individual radiation risk, we proposed to replace the average glandular dose by the imparted energy, which depends on the quantity and distribution of the glandular tissue, which is the tissue at risk. The volumetric imparted energy is computed from Monte Carlo simulations. The VBD, computed for the 0° projection of tomosynthesis exams, helps us to localize the glandular tissue and to attribute the imparted energy to the different tissues. A proposition was implemented for geometric phantoms, a textured phantom and a patient case.

Risk assessment models developed from administrative and clinical databases are used for clinical decision making. Since these models are derived from a database, they have an inherent limitation of being as good as the data they are derived from. Many of these models under or overestimate certain clinical outcomes particularly mortality in certain group of patients. Undeniably, there is significant variability in all these models on account of patient population studied, the statistical analysis used to develop the model and the period during which these models were developed. This review aims to shed light on development and application of risk assessment models for cardiac surgery with special emphasis on risk stratification in severe aortic stenosis to select patients for transcatheter aortic valve replacement.

Electrical bioimpedance spectroscopy (EBIS) has been used to assess the status or composition of various types of tissue, and examples of EBIS include body composition analysis (BCA) and tissue characterisation for skin cancer detection. EBIS is a non-invasive method that has the potential to provide a large amount of information for diagnosis or monitoring purposes, such as the monitoring of pulmonary oedema, i.e., fluid accumulation in the lungs. However, in many cases, systems based on EBIS have not become generally accepted in clinical practice. Possible reasons behind the low acceptance of EBIS could involve inaccurate models; artefacts, such as those from movements; measurement errors; and estimation errors. Previous thoracic EBIS measurements aimed at pulmonary oedema have shown some uncertainties in their results, making it difficult to produce trustworthy monitoring methods. The current research hypothesis was that these uncertainties mostly originate from estimation errors. In particular, time-varying behaviours of the thorax, e.g., respiratory and cardiac activity, can cause estimation errors, which make it tricky to detect the slowly varying behaviour of this system, i.e., pulmonary oedema. The aim of this thesis is to investigate potential sources of estimation error in transthoracic impedance spectroscopy (TIS) for pulmonary oedema detection and to propose methods to prevent or compensate for these errors.   This work is mainly focused on two aspects of impedance spectrum estimation: first, the problems associated with the delay between estimations of spectrum samples in the frequency-sweep technique and second, the influence of undersampling (a result of impedance estimation times) when estimating an EBIS spectrum. The delay between frequency sweeps can produce huge errors when analysing EBIS spectra, but its effect decreases with averaging or low-pass filtering, which is a common and simple method for monitoring the time-invariant behaviour of a system. The results show the importance of the undersampling effect as the main estimation error that can cause uncertainty in TIS measurements.  The best time for dealing with this error is during the design process, when the system can be designed to avoid this error or with the possibility to compensate for the error during analysis. A case study of monitoring pulmonary oedema is used to assess the effect of these two estimation errors. However, the results can be generalised to any case for identifying the slowly varying behaviour of physiological systems that also display higher frequency variations.  Finally, some suggestions for designing an EBIS measurement system and analysis methods to avoid or compensate for these estimation errors are discussed. / <p>QC 20140604</p>

The purpose of this study is to evaluate the value of systematic thoracic ultrasonography (TUS) for detecting bovine respiratory disease (BRD) related lung damage in Holstein x Angus crossbred calves. Because the dairy industry is known to operate on small profit margins, it is important to assess the potential of this technology to help prevent the main source of financial loss related to calf production that dairy producers face. Studies have shown that BRD may impact nearly a fourth of all dairy calves before weaning. In an industry that is currently growing and evolving, it is important that producers have all the necessary resources to operate efficiently. TUS is known to be a quick and accurate predictor of BRD related lung damage, but this study focuses on the financial implications of BRD related lung damage on calf growth and efficiency—average daily gain (ADG) and milk-to-gain (M:G)—and the value of implementing TUS information to make sound management decisions. TUS along with BRD diagnosis information give producers a unique perspective on future growth and development of calves and could be part of the solution to promote larger profit margins for dairy producers. We find that the value associated with TUS and BRD diagnosis information is between $0.88/head and $13.44/head and depends on BRD incidence rate, feed price, and feeder price. Depending on the cost to the farm, it may be beneficial to implement this as a way to manage BRD damage, which we know to influence calf growth and efficiency.

<p>Uvod - Etiologija sindroma gornjeg otvora grudnog ko&scaron;a (thoracic outlet syndrome- TOS) je udruženost vi&scaron;e faktora koji su posledica naru&scaron;avanja anatomsko-topografskih odnosa u nivou gornjeg otvora grudnog ko&scaron;a: kostoklavikularnog prostora, prednjeg skalenskog otvora, kao i same mehanike rebarnih zglobova i hrskavica, koji dovode do suženja prostora kroz koje prolaze neurovaskularne strukture, njihove kompresije, a samim tim i iritacije neurovaskularnih struktura. Sindrom gornjeg otvora grudnog ko&scaron;a je kompleks simptoma uzrokovanih kompresijom brahijalnog spleta, vene subklavije, arterije subklavije i simpatičkih vlakana, koji karakteri&scaron;u bol, parestezije, mi&scaron;ićna slabost i osećaj nelagodnosti u ruci/rukama koji se pojačava podizanjem ruke/ruku ili prekomernim pokretima glave i vrata, te dovode do smanjenja funkcionalne sposobnosti ruke/ruku i pote&scaron;koća u obavljanju svakodnevnih aktivnosti. Pored toga imaju često izraženu tahikardiju, osećaj stezanja u grudima, glavobolju, vrtoglavicu, zujanje u u&scaron;ima. Navedene tegobe su izraženije ponekad noću i dovode do poremećaja spavanja (problemi usnivanja, hrkanje, ka&scaron;ljanje, osećaj toplo/hladno, apnea, poremećaj dnevno/noćnog ritma- hronotipizacija, itd). Kao posledica lo&scaron;eg spavanja moguća je pojava depresivnih simptoma. U raspoloživoj literaturi, spavanje i kvalitet spavanja se uglavnom posmatraju kroz prizmu drugih bolesti i stanja. Materijal i metode - Istraživanjem je obuhvaćeno ukupno 181 (sto osam deset jedna) osoba. Od tog broja 53 mu&scaron;karca i 128 žena. Test grupu sačinjavalo je 82 ispitanika sa dijagnostikovanim TOS, a 99 zdravih osoba/osoba oba pola koji nemaju simptomatologiju TOS, je predstavljalo kontrolnu grupu. Grupe su bile ujednačene po polu i starosti (od 18 do 65). Tokom studije ispitanici su podvrgnuti kliničkom pregledu &ndash; fizikalnom i neurolo&scaron;kom pregledu. Pregled je podrazumevao pregled posture obolelog (posmatranje mi&scaron;ića regije vrata, ramena i ruku- trofika, tonus, konzistencija, mobilnost i kontraktilnost), posmatranje promena na koži (sa posebnim osvrtom na promene boje kože, trofičkih promena kože i noktiju i temperature kože ruku - &scaron;aka), testiranje refleksa, ispitivanje senzibiliteta u regijama inervacije odgovarajućih spinalnih korenova brahijalnog spleta. Takođe vr&scaron;ena je analiza stanja uhranjenosti (telesna visina, telesna masa, indeks telesne mase) i izvođenje provokativnih testova (Adson manevar, Rus test, Halsted test, Elvi test i Kostoklavikularni test). Kao deo ispitivanja obavljeno je i radiolo&scaron;ko snimanje vratne kičme kao i pregled oscilografom. Ispitanici su imali za zadatak da ispune sledeće upitnike: Upitnik o nesposobnosti ruke, ramena i &scaron;ake (DASH), Pitsbur&scaron;ki indeks kvaliteta spavanja (PSQI), Upitnik o hronotipizaciji (MEQ), Bekov upitnik o depresiji (BDI II). Na kraju ispitanici su davali odgovore na pitanja iz vodiča za istraživača sastavljenog sa ciljem evaluacije različitih aspekata spavanja. Rezultati - Rezultati su pokazali da su upotrebljene skale dale zadovoljavajuću pouzdanost. Pokazalo se da osobe sa dijagnozom TOS pored bogate simptomatologije imaju jo&scaron; i probleme sa spavanjem. Analizom rezultata do&scaron;lo se do zaključka da osobe sa dijagnozom TOS su starije, imaju vi&scaron;e problema sa spavanjem i veću nesposobnost ruke, ramena i &scaron;ake. Nije se pokazala razlika u hronotipizaciji kod osoba sa TOS u odnosu na kontrolnu grupu. Takođe može se zaključiti da osobe koje imaju izraženu nesposobnost ruku, ramena i &scaron;ake imaju značajno veću &scaron;ansu da imaju sindrom gornjeg otvora grudnog ko&scaron;a. Ove osobe pri tom imaju i veću &scaron;ansu da razviju depresiju i poremećaj spavanja, te se zaključuje da ova dva parametra svoj uticaj na TOS ostvaruju preko problema sa funkcionisanjem ruke, ramena i &scaron;ake. Dobar prediktor za postavljanje dijagnoze TOS može biti testiranje osoba DASH skalom. Zaključak - Rezultati studije mogu biti putokaz daljim istraživanjima, koja bi otvorila vrata formiranju protokola i opservaciji kvaliteta života osoba sa sindroma gornjeg otvora grudnog ko&scaron;a, sa posebnim osvrtom na poremećaje spavanja.</p> / <p>Etiology of thoracic outlet syndrome (TOS) is an association of several factors which are the result of disruption in the anatomical-topographical relations, in the level of superior thoracic aperture: costoclavicular space, anterior scalene aperture, as well as the mechanics of rib joints and cartilage, causing narrowing of space through which the neurovascular structures pass, their compression, and thus the irritation of neurovascular structures. Thoracic outlet syndrome is a complex of symptoms caused by the compression of the brachial plexus, subclavian vein, subclavian artery and sympathetic fibres, which is characterized by pain, parasthesias, muscle weakness and a feeling of discomfort in the arm / arms, which increases with raising the arm/arms or by excessive head and neck movements leading to a reduction of functional capabilities of the hands / arms and difficulties in everyday activities. In addition, patients often have expressed tachycardia, feeling of tightness in the chest, headache, dizziness, tinnitus. These problems are more pronounced at night and sometimes lead to sleep disorders (difficulty in falling asleep, snoring, coughing, feeling hot/cold, apnea, day/night rhythm disorder - Morningness/Eveningness disorder, etc.). As a result of poor sleeping depressive symptoms may occur. In the available bibliography, sleep and quality of sleep are mainly viewed through other diseases and conditions. The study included a total number of 181 (one hundred eighty one) people. Out of that 53 men and 128 women. The test group consisted of 82 patients diagnosed with TOS, and 99 healthy persons of both genders who had no symptoms of TOS, and constitute the control group. Groups were equalled by gender and age (18 to 65). Throughout the study subjects underwent clinical examination - both physical and neurological. This included an assessment of posture of the patient (observation of muscles in region of the neck, shoulders and arms - trophic, muscle tone, consistency, mobility and contractility), observation of changes on the skin (with a special emphasis on skin colour changes, trophic changes of the skin, nails and skin temperature of arms - hands), reflex testing, sensitivity testing in regions of innervations which correspond to spinal roots of the brachial plexus. Moreover, an analysis was conducted on the body composition analysis (body height, body weight, body mass index) as well as provocative tests (Adson manoeuvre, the Roos test, Halstead test, Elvy test and Costoclavicular test). Radiology scan of the cervical spine as well as oscillograph testing was conducted as a part of the test. The subjects were asked to fulfil the following questionnaires: Questionnaire on Disabilities of the Arm, Shoulder and Hand (DASH), Pittsburgh Sleep Quality Index (PSQI), The Morningness / Eveningness Questionnaire (MEQ), Beck Questionnaire on Depression (BDI II). Finally the subjects answered questions that researcher had drawn up in order to evaluate different aspects of sleep. The results showed that the scales used were reliable. It proved that persons diagnosed with TOS in addition to numerous symptoms also had problems with sleep. By analyzing the results it was concluded that people diagnosed with TOS are older, have more problems with sleep and greater disability of arms, shoulders and hands. There was no difference in Morningness/Eveningness type in patients with TOS in comparison to the control group. It can also be concluded that people who have pronounced disability of arms, shoulders and hands have a significantly greater chance of having thoracic outlet syndrome. These persons are more likely to develop depression and sleep disorders therefore those two entities impact TOS through the problems with the functioning of the arms, shoulders and hands. A good predictor in diagnosis of TOS may be testing people with DASH scale. The study findings can serve as a guideline for further research, opening the door into forming protocols and observation of the quality of life of people with the thoracic outlet syndrome, with special emphasis on sleep disorders.</p>

<p>Karcinomi pluća su najče&scaron;ći uzrok oboljevanja i umiranja od malignih tumora u Svetu. Neodjuvantna terapija kod bolesnika sa lokalno uznapredovalim (IIIA-IIIB) karcinomom pluća i zahvaćenim N2 limfnim čvorovima jedan je od modusa multimodalnog lečenja bolesnika sa nemikrocelularnim karcinomima pluća (NSCLC) u cilju pobolj&scaron;anja ishoda njihovog lečenja. Ovakav pristup podrazumeva prevođenje bolesnika iz vi&scaron;eg u niži stadijum bolesti - &bdquo;downstaging&rdquo;. Do danas nije utvrđena povezanost između pojedinih obrazaca tumorskog odgovora i vrste terapije. S obzirom na značaj kompletnog patolo&scaron;kog odgovora i tumorske regresije u prognozi ishoda lečenja, iznalaženje ove povezanosti je od značaja za dizajniranje budućih neoadjuvantnih trajala. Prilikom utvrđivnja histolo&scaron;ke slike tumorske regresije veoma je važno i merenje areje rezidualnog tumora (ART). Kako je veličina tumora jedan od prognostičkih faktora za bolesnike sa NSCLC koji nisu primali neoadjuvantnu terapiju tako je i merenje ART, za razliku od makroskopske veličine tumora, jedan od prognostičkih faktora za bolesnike sa NSCLC koji su primali neoadjuvantnu terapiju. Krajnji cilj neoadjuvantne terapije trebalo bi da bude resektabilnost i &bdquo;downstaging&rdquo; koji bi mogao da obezbedi u specifičnim kliničkim situacijama i sveukupni onkolo&scaron;ki benefit. Osnovni ciljevi ove doktorske disertacije su bili: da se objektivizira procena veličine ART u tumorskom tkivu pluća i limfnih čvorova; da se proceni povezanost povr&scaron;ine ART sa veličinom tumora na postoperativnom hirur&scaron;kom materijalu posle neoadjuvantne terapije; da se analizira i proceni povezanost histomorfolo&scaron;kih parametara kod tumorske regresije indukovane neoadjuvantnom terapijom i spontane tumorske regresije u tumorima pluća i limfnih čvorova na&nbsp; postoperativnom hirur&scaron;kom materijalu i u zavisnosti od histolo&scaron;kog tipa karcinoma; da se proceni povezanost kliničkog odgovora na neoadjuvantnu terapiju prema kriterijumima Svetske Zdravstvene Organizacije i histolo&scaron;kih parametara u tumorima pluća i limfnim čvorovima na postoperativnom hirur&scaron;kom materijalu nakon neoadjuvantne terapije; da se proceni povezanost patolo&scaron;kog ypTN sa kliničkim ycTN stadijumom bolesti i stepena tumorske regresije indukovane neoadjuvantnom terapijom i patolo&scaron;kog ypTN i da se proceni povezanosti između kliničke i patolo&scaron;ke zahvaćenosti N2 limfnih čvorova posle neoadjuvantne terapije. Merenje ukupne veličine očuvanih ART je najznačajniji objektivni parametar u proceni stepena tumorske regresije. Veličina rezidualnog tumora nije u korelaciji sa veličinom tumora posle neoadjuvantne terapije. Postoji signifikantna razlika u patohistolo&scaron;koj slici tumorske regresije indukovane neoadjuvantnom terapijom i spontane tumorske regresije. Ne postoji signifikantna razlika između histolo&scaron;kog tipa tumora i histolo&scaron;ke slike tumorske regresije. Ne postoji signifikantna povezanost između kliničkog odgovora i stepena tumorske regresije nakon neoadjuvantne terapije. Ne postoji korelacija između kliničkog i patolo&scaron;kog stadijuma bolesti posle neoadjuvantne terapije. Ne postoji korelacija između stepena tumorske regresije indukovane neoadjuvantnom terapijom i ypTN stadijuma bolesti. Ne postoji korelacija između kliničke i patolo&scaron;ke zahvaćenosti N2 limfnih čvorova posle neoadjuvantne terapije. Stepen regresije tumora i merenje ART posle neoadjuvantne terapije određen histopatolo&scaron;kom analizom reseciranog tumora je najobjektivniji kriterijum za procenu hemioterapijskog odgovora i predviđanja ishoda lečenja pacijenata.</p> / <p>Lung cancers are the most common cause of morbidity and mortality from malignant tumors in the World. The neodjuvant therapy in patients with locally advanced (IIIA-IIIB) lung cancer and affected N2 lymph nodes is one of the modes of multimodal treatment of patients with non-small cell lung cancer (NSCLC) in order to improve the outcome of their treatment. This involves converting patients from a higher to a lower stage of the disease - &quot;downstaging&quot;. There has been no significant connection between some forms of tumor response and types of therapy. Given the importance of complete pathological responses and tumor regression in the prediction of treatment outcomes, finding this relationship is of importance for the design of future neoadjuvant trails. In determining the histological tumor regression is very important measurement of area of residual tumor (ART). As the size of the tumor is one of the prognostic factors in patients with NSCLC who did not receive neoadjuvant therapy so the measurement of ART, as opposed to the macroscopic size of the tumor, one of the prognostic factors in patients with NSCLC, who had received neoadjuvant therapy. The ultimate goal of neoadjuvant therapy should be resectability and &quot;downstaging&quot; that could provide overall oncology benefit in specific clinical situations. The main objectives of this thesis were: to objectively estimate the size of ART in tumor tissue of lung and lymph nodes; to estimate the relation between the surface of ART with the size of the tumor on postoperative surgical material after neoadjuvant therapy; to analyze and estimate the relation between histomorphological parameters in tumor regression induced by neoadjuvant therapy and spontaneous tumor regression in tumors of the lung and lymph nodes in the postoperative surgical material and depending on the histological type of cancer; to estimate the relation between clinical response to neoadjuvant therapy according to criteria of the World Health Organization and histological parameters in lung tumors and lymph nodes in the postoperative surgical material after neoadjuvant therapy; to estimate the correlation of the pathological ypTN with clinical ycTN stage of the disease and the degree of tumor&nbsp; regression induced by neoadjuvant therapy and pathological ypTN and estimation of the relation between clinical and pathological involvement of N2 lymph nodes after neoadjuvant therapy. Measurement of the total size of the preserved ART is the most important objective parameter in the assessment of the grade of tumor regression. Size of residual tumor did not correlate with the size of the tumor after neoadjuvant therapy. There was a significant difference in the histological picture of tumor regression induced by neoadjuvant therapy and spontaneous tumor regression. There was no significant difference between the histologic type of tumor and histological tumor regression. There is no significant correlation between clinical response and the grade of tumor regression after neoadjuvant therapy. There is no correlation between clinical and pathological staging of the disease after neoadjuvant therapy. There is no correlation between the grade of tumor regression induced by neoadjuvant therapy and ypTN stage of the disease. There is no correlation between the clinical and the pathological involvement of the N2 lymph nodes to neoadjuvant therapy. The grade of tumor regression and measurement ART after neoadjuvant therapy determined by histopathological analysis of the resected tumor is the most objective criterion for evaluation of chemotherapeutic response and prediction of treatment outcome in patients.</p>

<p>Cilj: Proveriti da li je moguće promeniti način CT verifikacije Hallerovog indeksa u okviru preoperativne pripreme pacijenata sa deformitetom grudnog ko&scaron;a po tipu pectus excavatum-a, uz utvrđivanje fiziolo&scaron;kog opsega vrednosti indeksa, njegove zavisnosti od uzrasta i pola, kao i respiratorne faze u kojoj se CT pregled izvodi. Utvrditi značaj vrednosti indeksa korekcije, indeksa asimetrije i indeksa torzije sternuma. Proveriti i mogućnost smanjivanja efektivne doze jonizujućeg zračenja kod primene CT tehnike jednog skena (single slice). Materijal i metod rada: Ispitivanje se sastojalo iz prospektivne studije koja je obuhvatila 30 pacijenata sa pektus ekskavatumom uz CT snimanje (single slice) u respiratornim fazama: ekspirijum i inspirijum. Na dobijenim snimcima su izračunati pored Halerovog indeksa i indeksi asimetrije, korekcije i sternalne torzije. Prema dobijenim rezultatima merenja i izračunavanja, dono&scaron;ena je odluka o načinu terapijskog tretmana. U retrospektivnoj studiji, urađena je analiza 100 CT pregleda grudnog ko&scaron;a kod dece gde nije verifikovan pectus excavatum, uz kalibriranje vrednosti indeksa u odnosu na uzrast i pol pacijenata. Retrospektivno istraživanje je obuhvatilo i analizu dodatnih 30 CT pregleda grudnog ko&scaron;a pacijenata sa deformitetom grudnog ko&scaron;a po tipu pectus excavatum-a, koji su dijagnostikovani i/ili operisani. Kod svih operisanih pacijenata je primenjenja Nusova operativna tehnika. U ovoj grupi pacijenata je izračunata srednja vrednost efektivne doze jonizujućeg zračenja, a dobijene vrednosti su potom komparirane sa efektivnim dozama koje su dobijene niskodoznim CT pregledima grudnog ko&scaron;a uz primenu novog protokola (&quot;single-slice&quot; tehnika u respiratornim fazama inspirijuma i ekspirijuma). Rezultati: Fiziolo&scaron;ki opseg vrednosti Haller-ovog indeksa u populaciji zdravih pacijenata se kretao od 1,47 do 3,17 i u proseku je iznosio 2,23&plusmn;0,32 (znatna pozitivna korelacija uzrasta i vrednosti HI). Na osnovu rezultata Man-Vitnijevog testa nije postojala polna razlika u posmatranoj grupi. Haller-ov indeks u grupi dijagnostikovane/operisane dece je u proseku iznosio 3,39 i kretao se u intervalu od 2,23 do 5,72 (korelacija između uzrasta ovih pacijenata i Haller-ovog indeksa je bila neznatna i negativna). Utvrđena je zavisnost veličine Haller-ovog indeksa od respiratorne faze u kojoj se CT pregled izvodi. Tako su prosečne vrednosti Haller-ovog indeksa u inspirijumu dece sa dijagnostikovanim deformitetom iznosile 2,69&plusmn;0,76. Kod dece ove grupe u ekspirijumu vrednosti Haller-ovog indeksa su iznosile 3,49&plusmn;1,19. U inspirijumu su samo 3/32 (9%) ispitanika imali vrednost HI preko 3,25 (granična vrednost za operativni tretman), dok ih je u ekspirijumu bilo znatno vi&scaron;e 13/32 (41%), &scaron;to je statistički značajna razlika (&chi;2=6,250; df =1; p=0,012). &quot;Single-slice&quot; tehnika CT pregleda u inspirijumu i ekspirijumu 20-25 puta smanjuje efektivnu dozu jonizujućeg zračenja. Zaključak: Vrednost Haller-ovog indeksa raste sa uzrastom deteta, dok nije utvrđena zavisnost Haller-ovog indeksa od pola. Moguće je promeniti načini CT verifikacije Haller-ovog indeksa u preoperativnoj pripremi za Nuss-ovu operaciju primenom &quot;single-slice&quot; tehnike u ekspiratornoj fazi. Osim Hallerovog indeksa, korisno je određivati i indeks korekcije, indeks asimetrije i indeks sternalne rotacije. Predlaže se protokol standardne preoperativne pripreme i lečenja pacijenata dečjeg uzrasta sa deformitetom grudnog ko&scaron;a po tipu pektus ekskavatuma, sa ciljem njegove primene u svakodnevnom radu u institucijama koje se bave ovim problemom, a u cilju pobolj&scaron;anja kvaliteta dijagnostikovanja i krajnjeg ishoda lečenja.</p> / <p>Aim: The aim of this study was to verify whether it is possible to change the way of CT verification of Haller index (HI), as part of preoperative preparation for patients with pectus excavatum, with the determination of the physiological range of the index value, its dependence on the age and sex, as well as the respiratory phase during which the scan is performed. Also, the aim was to determine the significance of correction, asymmetry and sternal torsion indices values. Evaluate the possibility of reduction the effective dose of ionizing radiation using a single slice CT scan technique. Materials and methods: The study consisted of prospective study that included evaluation of CT scans (single slice technique) of 30 patients with pectus excavatum in both respiratory phases: expirium and inspirium. Haller index and indices of asymmetry, correction and sternal torsion were measured. The decision for the treatment was made according to the results of measurements and calculations of these indices. In retrospective study, 100 CT scans of the chest in children without the deformity (pectus excavatum) were analyzed, and the index value was calibrated depending on the age and gender. The retrospective study also included the analysis of another 30 CT scans in patients who were operated or diagnosed with pectus excavatum. Nuss procedure was used in all operated patients. In this group of patients the median value of effective dose of ionizing radiation was calculated, and the values were compared with the effective dose obtained using low-dose CT examinations applied in the new protocol (single-slice technique in inspiratory and expiratory respiratory phases). Results: The physiological range of Haller index value in healthy patients was from 1.47 to 3.17 and average value was 2.23&plusmn;0.32 (significant positive correlation between age and the value of HI). Results of Mann- Whitney test did not demonstrate any difference between gender in the observed group. In the group of patients who were operated/diagnosed with pectus excavatum the average value of Haller index was 3.39 within the range of 2.23 to 5.72 (correlation between the age of these patients and Haller index was negative, but not significant). The dependence of the Haller index value and certain respiratory phase during which the CT scan was performed also was determined. Thus, the average value of Haller index in inspirium in children with diagnosed deformity was up to 2.69&plusmn;0.76. In the same group of patients the value of Haller index in expirium was up to 3.49&plusmn;1.19. Only 3/32 (9%) patients had HI value over 3.25 (a boundary value for surgical treatment) during inspirium, while 13/32 (41%) patients had it in expirium, this data showed statistically significant difference (&chi;2=6.250; df=1; p=0.012). Single-slice CT technique during the inspiratory and expiratory phase reduces 20-25 times the effective dose of ionizing radiation. Conclusion: The value of Haller index increases with the age, but its dependence on the gender was not determined. It is possible to change the way of CT verification of Haller index in preoperative preparation for the Nuss operation using the &quot;single-slice&quot; technique in exspiratory phase. In addition to Haller index it is useful to determine correction index, the index of asymmetry and the index of sternal rotation as well. This protocol is proposed for standard preoperative preparation and treatment of pediatric population with pectus excavatum with the aim of its application in daily work in institutions that deal with this problem, but also to improve the quality of diagnosis and treatment outcomes.</p>

INTRODUÇÃO: Para os pacientes portadores de transposição das grandes artérias que perderam a chance da cirurgia de Jatene nas primeiras semanas de vida, indica-se realizar o preparo ventricular através da bandagem do tronco pulmonar (BTP), objetivando causar aumento na massa miocárdica. Entretanto, com o tempo, a câmara hipertrofiada pode apresentar disfunção contrátil; portanto, é importante conhecer a qualidade do tecido preparado, uma vez que já se sabe que tanto os miocardiócitos (MCD) quanto as células do interstício e vasos (I/V) são capazes de proliferar após o período neonatal. Baseando-se no condicionamento físico de atletas e considerando-se que os músculos cardíaco e esquelético são ambos estriados, postula-se a hipótese de que o tipo de preparo ventricular possa influenciar nas características do miocárdio treinado. OBJETIVOS: Identificar o tipo de mecanismo adaptativo (hipertrofia/hiperplasia) envolvido no preparo rápido do ventrículo pulmonar submetido à sobrecarga de pressão por meio de BTP, através da análise dos MCD e células do I/V, verificando se existem diferenças em relação ao tipo de treinamento (contínuo x intermitente) em comparação com os controles. MÉTODOS: Foram estudados experimentalmente 21 cabritos após o período neonatal, divididos em três grupos (C = grupo controle, n = 7, sem procedimento cirúrgico; EC = grupo de estimulação contínua, n = 7, com bandagem progressiva e permanente do tronco pulmonar durante 96 horas; EI = grupo de estimulação intermitente, n = 7, com bandagem progressiva, 12 horas ao dia, totalizando 48 horas). Todos foram submetidos a estudo ecocardiográfico basal e aqueles dos grupos EC e EI a ecocardiogramas diários para verificar a aquisição de massa muscular do ventrículo direito (VD). Após o estudo, os animais foram sacrificados, os corações retirados e cortes histológicos do VD, ventrículo esquerdo (VE) e septo interventricular (S) fixados em formalina e processados para análise. Foram estudados a porcentagem de área de colágeno através do Picro-sirius, o diâmetro dos MCD e seus respectivos núcleos e o número de MCD e células do I/V marcadas com Ki-67. As células marcadas foram avaliadas por campo microscópico e por índice (número de células Ki-67+/2000 células). O nível de significância considerado foi de 0,05. RESULTADOS: Ambos os grupos estimulados apresentaram ganho significativo de massa muscular do VD (p < 0,05). Não houve aumento na porcentagem de colágeno do VD nos grupos treinados (p = 0,403). Considerando-se o VD, os grupos EC e EI apresentaram diâmetro dos MCD maior que o grupo controle (p < 0,001), ocorrendo o mesmo com os respectivos núcleos (EI x C: p < 0,001 e EC x C: p = 0,005). O número de MCD marcados com Ki-67 foi maior no VD dos grupos estimulados comparado com o VE (p = 0,009, índice de proliferação; p = 0,001, contagem por campo), bem como para as células do I/V (p < 0,001, contagem por campo e índice). CONCLUSÕES: Tanto hipertrofia quanto hiperplasia celular estão envolvidas na adaptação do ventrículo pulmonar submetido à sobrecarga sistólica através da BTP. Ambos os tipos de condicionamento (contínuo e intermitente) provocaram hipertrofia e hiperplasia dos MCD, induziram também à mitose das células do I/V, sem deposição de colágeno intersticial ao final do experimento. / INTRODUCTION: Rapid ventricular conditioning induced by pulmonary artery banding (PAB) has been indicated to those patients with transposition of the great arteries (TGA) who have lost the chance for arterial switch operation ? Jatene?s procedure ? aiming at induce myocardial mass increase. However, with time, hypertrophied chamber may exhibit contractile dysfunction, so that, it is important to assess quality of the prepared tissue, once it is of knowledge that both cardiomyocytes (CMC) and interstitial/vessel (I/V) cells are capable of proliferating after neonatal period. Based on fitness of athletes and considering that cardiac and skeletal muscles are both striated, there is the hypothesis that the type of ventricular prepare may influence the characteristics of the training myocardium. OBJECTIVES: Through CMC and I/V cells analysis, identifies the type of adaptive mechanism (hypertrophy/ hyperplasia) involved in rapid prepare of subpulmonary ventricle submitted to pressure overload by PAB, and verifies if there are differences in relation to the kind of training (continuous x intermittent), comparing them to the controls. METHODS: Twenty-one goats, beyond neonatal period, were experimentally studied. They were divided in three groups: C (control group, n = 7, with no surgical procedure); CS (continuous stimulation group, n = 7, with progressive and permanent PAB, during 96 hours); IS (intermittent stimulation group, n = 7, with progressive PAB, 12 hours/day, totalizing 48 hours). All the animals were submitted to basal echocardiograms and those from CS and IS groups to diary echocardiograms to verify right ventricular (RV) muscular mass acquisition. After the study, goats were killed, hearts excised and histological sections from RV, left ventricle (LV) and ventricular septum (VS) were formalin fixed and histologically processed. Collagen area fraction (through Picro-sirius red staining), CMC and respective nuclei diameter, and number of CMC and I/V cells Ki-67 positive were studied. Marked cells were analysed per high-power fields and by index (Ki-67 positive cells/2000 cells). The statistical significant level was set at 5%. RESULTS: Both stimulated groups presented significant RV muscular mass increase (p < 0.05). There were no augmentation in RV collagen area fraction in training groups (p = 0.403). Considering the RV, CS and IS groups showed an increase in CMC diameter compared to the control group (p < 0.01), occurring the same to respective nuclei (EI x C: p < 0.001 e EC x C: p = 0.005). Number of CMC marked with Ki-67 was greater in RV from stimulated groups in relation to LV (p = 0.009, proliferation index; p = 0.001, number/high-power fields); the same occurred to I/V cells (proliferation index and number/high-power fields: p < 0.001). CONCLUSIONS: Both cell hypertrophy and hyperplasia are involved in adaptation of the pulmonary ventricle submitted to pressure overload through PAB. Both types of conditioning (continuous and intermittent) caused CMC hypertrophy and hyperplasia, besides induced I/V cells mitosis, without interstitial collagen deposition at the end of experiment.